primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16_p1 rps16 1 TGGCACGACATAAATCCAGT TCCCTATACCACTTTGCAATGA 20 22 45.0 40.9 57.2 57.1 0.0 18.3 3.633223 4521 5607 1247-1248 1.000000 51.4 rps16_p2 rps16 2 TGGCACGACATAAATCCAGT TCAATTCCCTATACCACTTTGCA 20 23 45.0 39.1 57.2 57.8 0.0 0.0 3.966011 4521 5607 1252-1253 1.000000 48.8 rps16_p3 rps16 3 TGGCACGACATAAATCCAGT TCCCTATACCACTTTGCAATGAA 20 23 45.0 39.1 57.2 57.8 0.0 18.3 3.966011 4521 5607 1247-1248 1.000000 48.8 rps16_p4 rps16 4 TGGCACGACATAAATCCAGT TTCCCTATACCACTTTGCAATGA 20 23 45.0 39.1 57.2 57.8 0.0 18.3 3.966011 4521 5607 1248-1249 1.000000 48.8 rps16_p5 rps16 5 TGGCACGACATAAATCCAGT CCCTATACCACTTTGCAATGA 20 21 45.0 42.9 57.2 55.6 0.0 18.3 4.140464 4521 5607 1246-1247 1.000000 47.4 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 5608 7140 1603-1609 1.000000 78.9 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 0.0 16.9 0.280333 5608 7140 1614-1620 1.000000 78.4 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 20 20 45.0 55.0 58.1 57.8 8.1 16.9 0.350677 5608 7140 1607-1613 1.000000 77.8 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 20 20 50.0 55.0 58.2 57.8 0.0 16.9 0.415585 5608 7140 1618-1624 1.000000 77.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 AAGTCGCACGTTGCTTTCTA TAAAACCAGAAATGGGGCGT 20 20 45.0 45.0 58.1 57.7 8.1 0.0 0.435397 5608 7140 1663-1669 1.000000 77.1 trnS-GCU-psbD_p1 trnS-GCU-psbD 1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 20 20 45.0 50.0 58.0 58.2 0.0 5.0 0.209227 8475 9457 1126-1129 1.000000 79.4 trnS-GCU-psbD_p2 trnS-GCU-psbD 2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 20 20 45.0 45.0 58.0 58.2 0.0 0.0 0.284276 8475 9457 1113-1116 1.000000 78.8 trnS-GCU-psbD_p3 trnS-GCU-psbD 3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 20 20 50.0 50.0 57.6 58.2 0.9 5.0 0.580969 8475 9457 1112-1115 1.000000 76.4 trnS-GCU-psbD_p4 trnS-GCU-psbD 4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 20 20 45.0 55.0 58.0 58.6 0.0 0.0 0.605692 8475 9457 1105-1108 1.000000 76.2 trnS-GCU-psbD_p5 trnS-GCU-psbD 5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.6 58.2 0.9 0.0 0.656018 8475 9457 1099-1102 1.000000 75.8 trnG-UCC_p1 trnG-UCC 1 CAAACTGCTCTACTCCGCTC CTCGAATGGCTACCCTTGAC 20 20 55.0 55.0 58.4 58.1 0.0 0.0 0.423973 13607 14350 906-906 1.000000 76.7 trnG-UCC_p2 trnG-UCC 2 CAAACTGCTCTACTCCGCTC CGAATGGCTACCCTTGACAA 20 20 55.0 50.0 58.4 57.9 0.0 0.0 0.473248 13607 14350 904-904 1.000000 76.3 trnG-UCC_p3 trnG-UCC 3 TGGTGGACGAAAAAGGTTGA CTCGAATGGCTACCCTTGAC 20 20 45.0 55.0 57.6 58.1 0.0 0.0 0.480426 13607 14350 871-871 1.000000 76.2 trnG-UCC_p4 trnG-UCC 4 AACTGGTGGACGAAAAAGGT CTCGAATGGCTACCCTTGAC 20 20 45.0 55.0 57.6 58.1 0.0 0.0 0.488923 13607 14350 874-874 1.000000 76.2 trnG-UCC_p5 trnG-UCC 5 ACTGGTGGACGAAAAAGGTT CTCGAATGGCTACCCTTGAC 20 20 45.0 55.0 57.6 58.1 0.0 0.0 0.488923 13607 14350 873-873 1.000000 76.2 trnG-UCC-trnT-GGU_p1 trnG-UCC-trnT-GGU 1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 21 21 38.1 52.4 55.5 58.1 0.0 0.0 4.684647 14351 16313 1475-2143 1.000000 47.1 trnG-UCC-trnT-GGU_p2 trnG-UCC-trnT-GGU 2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 21 21 38.1 47.6 55.5 57.8 0.0 13.0 4.776909 14351 16313 1488-2156 1.000000 46.3 trnG-UCC-trnT-GGU_p3 trnG-UCC-trnT-GGU 3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 21 21 38.1 47.6 55.5 57.8 0.0 13.0 4.776909 14351 16313 1489-2157 1.000000 46.3 trnG-UCC-trnT-GGU_p4 trnG-UCC-trnT-GGU 4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 23 21 39.1 52.4 57.3 58.1 0.0 0.0 4.817363 14351 16313 1478-2146 1.000000 46.0 trnG-UCC-trnT-GGU_p5 trnG-UCC-trnT-GGU 5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 23 21 39.1 52.4 57.3 58.1 0.0 0.0 4.817363 14351 16313 1477-2145 1.000000 46.0 rpoB_p1 rpoB 1 AGAAGGATTTGGTGTTGCTCA TTGTTGAGGGGAAACGAGTC 21 20 42.9 50.0 57.8 57.8 0.0 0.0 1.478032 24748 24867 322-322 1.000000 68.7 rpoB_p2 rpoB 2 AATGCTACGATCTGGGGAAA TTGTTGAGGGGAAACGAGTC 20 20 45.0 50.0 56.6 57.8 0.0 0.0 1.644877 24748 24867 240-240 1.000000 67.5 rpoB_p3 rpoB 3 AGAAGGATTTGGTGTTGCTCA GAGGGGAAACGAGTCCAATT 21 20 42.9 50.0 57.8 57.5 0.0 10.6 1.719863 24748 24867 317-317 1.000000 66.7 rpoB_p4 rpoB 4 AATGCTACGATCTGGGGAAA GAGGGGAAACGAGTCCAATT 20 20 45.0 50.0 56.6 57.5 0.0 10.6 1.886708 24748 24867 235-235 1.000000 65.6 rpoB_p5 rpoB 5 AGAAGGATTTGGTGTTGCTCA TGAGGGGAAACGAGTCCAAT 21 20 42.9 50.0 57.8 58.6 0.0 10.6 1.870036 24748 24867 318-318 1.000000 65.5 rpoC2_p1 rpoC2 1 ACCCTCCAAGTACTTCAAGG TGTCCAAGGTGAATTATTGCC 20 21 50.0 42.9 56.5 56.5 19.6 0.0 4.063924 30618 30737 161-161 1.000000 49.0 rpoC2_p2 rpoC2 2 AACCCTCCAAGTACTTCAAGG TGTCCAAGGTGAATTATTGCC 21 21 47.6 42.9 57.2 56.5 19.6 0.0 4.304936 30618 30737 162-162 1.000000 47.0 rpoC2_p3 rpoC2 3 ACCCTCCAAGTACTTCAAGG TTGTCCAAGGTGAATTATTGCC 20 22 50.0 40.9 56.5 57.2 19.6 0.0 4.348641 30618 30737 162-162 1.000000 46.7 rpoC2_p4 rpoC2 4 AACCCTCCAAGTACTTCAAGG TTGTCCAAGGTGAATTATTGCC 21 22 47.6 40.9 57.2 57.2 19.6 0.0 4.589653 30618 30737 163-163 1.000000 44.8 rpoC2_p5 rpoC2 5 AAACCCTCCAAGTACTTCAAGG TGTCCAAGGTGAATTATTGCC 22 21 45.5 42.9 57.9 56.5 19.6 0.0 4.615126 30618 30737 163-163 1.000000 44.6 ycf3_p1 ycf3 1 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGA 20 20 45.0 45.0 57.8 55.7 0.0 21.9 2.453412 44391 46385 2137-2144 0.909091 57.0 ycf3_p2 ycf3 2 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGA 20 20 45.0 45.0 57.8 55.7 0.0 21.9 2.453412 44391 46385 2136-2143 0.909091 57.0 ycf3_p3 ycf3 3 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGAT 20 21 45.0 42.9 57.8 56.3 0.0 29.8 2.890713 44391 46385 2137-2144 0.909091 53.5 ycf3_p4 ycf3 4 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGAT 20 21 45.0 42.9 57.8 56.3 0.0 29.8 2.890713 44391 46385 2136-2143 0.909091 53.5 ycf3_p5 ycf3 5 GATCAACAACCAAACCACCC AGAGCATATGGATCGATCGA 20 20 50.0 45.0 57.2 55.7 0.0 21.9 3.098864 44391 46385 2138-2145 0.909091 51.8 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.032006 48333 49151 918-937 1.000000 80.4 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073242 48333 49151 910-929 1.000000 80.1 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 48333 49151 888-907 1.000000 79.6 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 20 20 50.0 55.0 58.0 58.2 0.0 10.2 0.272624 48333 49151 908-927 1.000000 78.5 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 20 20 50.0 50.0 58.0 57.7 0.0 0.0 0.303758 48333 49151 917-936 1.000000 78.3 rbcL-psaI_p1 rbcL-psaI 1 AAATTTGAGTTCGAGCCGGT CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 58.1 58.0 1.9 26.9 0.134794 58367 59652 1394-1395 1.000000 79.6 rbcL-psaI_p2 rbcL-psaI 2 GCCGCAGCTTGTGAAATATG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.2 58.0 0.0 26.9 0.195661 58367 59652 1424-1425 1.000000 79.1 rbcL-psaI_p3 rbcL-psaI 3 AAATTTGAGTTCGAGCCGGT AAGAAGCCATTGCAATTGCC 20 20 45.0 45.0 58.1 57.9 1.9 26.8 0.211328 58367 59652 1400-1401 1.000000 79.0 rbcL-psaI_p4 rbcL-psaI 4 GCCGCAGCTTGTGAAATATG AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.2 57.9 0.0 26.8 0.272195 58367 59652 1430-1431 1.000000 78.5 rbcL-psaI_p5 rbcL-psaI 5 CAAATTTGAGTTCGAGCCGG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.8 58.0 13.1 26.9 0.274314 58367 59652 1395-1396 1.000000 78.5 rps18_p1 rps18 1 AGAATCGCCACAATACACCC CGTTTGTGGATCACTCGGAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.048532 68167 68679 1231-1273 1.000000 83.8 rps18_p2 rps18 2 CAAGCTAAGTCAGGCCTCAG CGTTTGTGGATCACTCGGAT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.031500 68167 68679 1604-1646 1.000000 83.0 rps18_p3 rps18 3 CATAGAAAGAGGCTGGGCTG CGTTTGTGGATCACTCGGAT 20 20 55.0 50.0 58.0 58.0 0.3 0.0 0.045593 68167 68679 1575-1617 1.000000 82.9 rps18_p4 rps18 4 CAAGCTAAGTCAGGCCTCAG GACGACGAACAAAAATGCGT 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.034641 68167 68679 1744-1786 1.000000 82.7 rps18_p5 rps18 5 CATAGAAAGAGGCTGGGCTG GACGACGAACAAAAATGCGT 20 20 55.0 45.0 58.0 58.0 0.3 0.0 0.048733 68167 68679 1715-1757 1.000000 82.7 petB_p1 petB 1 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.6 58.2 0.0 0.0 1.620707 73798 75263 1571-1575 1.000000 67.4 petB_p2 petB 2 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATG 20 20 50.0 50.0 57.6 56.7 0.0 0.0 1.668360 73798 75263 1571-1575 1.000000 67.0 petB_p3 petB 3 GGTAGTTCGACCGTGGAATT TGAAATACCACAACCTACCCG 20 21 50.0 47.6 57.6 57.7 0.0 0.0 1.712867 73798 75263 1577-1581 1.000000 66.7 petB_p4 petB 4 ATTCTGGTAGTTCGACCGTG ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.4 58.2 0.0 0.0 1.894865 73798 75263 1576-1580 1.000000 65.2 petB_p5 petB 5 CATTCTGGTAGTTCGACCGT ACCACAACCTACCCGATATGA 20 21 50.0 47.6 57.4 58.2 0.0 0.0 1.894865 73798 75263 1577-1581 1.000000 65.2 rpl14_p1 rpl14 1 TCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGA 21 21 38.1 33.3 53.4 52.8 0.0 0.0 11.801385 79804 80175 447-447 1.000000 41.1 rpl14_p2 rpl14 2 TCTTTGATCTATATCTCGCCTACT TCTTTCATCCTTTTGCATAGA 24 21 37.5 33.3 56.4 52.8 0.0 0.0 11.828114 79804 80175 447-447 1.000000 41.1 rpl14_p3 rpl14 3 TCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGAA 21 22 38.1 31.8 53.4 53.7 0.0 0.0 11.910981 79804 80175 447-447 1.000000 41.1 rpl14_p4 rpl14 4 TCTTTGATCTATATCTCGCCT TTCTTTCATCCTTTTGCATAGA 21 22 38.1 31.8 53.4 53.7 0.0 0.0 11.910981 79804 80175 448-448 1.000000 41.1 rpl14_p5 rpl14 5 TTCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGA 22 21 36.4 33.3 54.2 52.8 0.0 0.0 11.936627 79804 80175 448-448 1.000000 41.1 rpl14-rpl16_p1 rpl14-rpl16 1 AGCAGCTCCTATGACTCGAA TAGCTGCCAGTAAAATGCCC 20 20 50.0 50.0 58.2 58.2 0.0 0.0 0.468267 80176 80284 245-248 1.000000 78.2 rpl14-rpl16_p2 rpl14-rpl16 2 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 20 20 55.0 50.0 58.4 58.2 8.5 0.0 0.671988 80176 80284 211-214 1.000000 76.9 rpl14-rpl16_p3 rpl14-rpl16 3 TAGCAGCTCCTATGACTCGA TAGCTGCCAGTAAAATGCCC 20 20 50.0 50.0 57.4 58.2 0.0 0.0 0.877608 80176 80284 246-249 1.000000 74.9 rpl14-rpl16_p4 rpl14-rpl16 4 AGCAGCTCCTATGACTCGAA TGAAATGGGCGGAGTATCTG 20 20 50.0 50.0 58.2 57.4 0.0 0.0 0.851226 80176 80284 292-295 1.000000 74.8 rpl14-rpl16_p5 rpl14-rpl16 5 AGCAGCTCCTATGACTCGAA GCCAGTAAAATGCCCATACG 20 20 50.0 50.0 58.2 57.2 0.0 0.0 1.005413 80176 80284 240-243 1.000000 73.9 ndhH-ndhF_p1 ndhH-ndhF 1 AAAATCGCGGAAAACCGAAC AAATATGTAGGGGGAGGGCG 20 20 45.0 55.0 58.0 58.9 6.1 0.0 0.975890 105004 105111 228-233 1.000000 79.9 ndhH-ndhF_p2 ndhH-ndhF 2 TCGCGGAAAACCGAACTATT AAATATGTAGGGGGAGGGCG 20 20 45.0 55.0 57.9 58.9 6.1 0.0 1.018342 105004 105111 224-229 1.000000 79.7 ndhH-ndhF_p3 ndhH-ndhF 3 AATCGCGGAAAACCGAACTA AAATATGTAGGGGGAGGGCG 20 20 45.0 55.0 57.9 58.9 6.1 0.0 1.018342 105004 105111 226-231 1.000000 79.6 ndhH-ndhF_p4 ndhH-ndhF 4 AAAATCGCGGAAAACCGAAC GTAGGGGGAGGGCGAATAT 20 19 45.0 57.9 58.0 57.9 6.1 0.0 1.124906 105004 105111 222-227 1.000000 78.9 ndhH-ndhF_p5 ndhH-ndhF 5 AAAATCGCGGAAAACCGAAC GTAGGGGGAGGGCGAATATC 20 20 45.0 60.0 58.0 59.1 6.1 0.0 1.137478 105004 105111 222-227 1.000000 78.8 ndhF_p1 ndhF 1 GGAGAGATGGGGGAAGAAGA TGGTTTGGTGAATCGGAAACA 20 21 55.0 42.9 57.8 58.3 0.0 0.0 1.526513 105112 107331 2363-2363 1.000000 67.9 ndhF_p2 ndhF 2 GGAGAGATGGGGGAAGAAGA GGTTTGGTGAATCGGAAACA 20 20 55.0 45.0 57.8 56.5 0.0 0.0 1.638224 105112 107331 2362-2362 1.000000 67.0 ndhF_p3 ndhF 3 GGAGAGATGGGGGAAGAAGA TGGTTTGGTGAATCGGAAAC 20 20 55.0 45.0 57.8 56.5 0.0 0.0 1.638224 105112 107331 2363-2363 1.000000 67.0 ndhF_p4 ndhF 4 GGAGACGGGGGAATTCCTTA TGGTTTGGTGAATCGGAAACA 20 21 55.0 42.9 58.5 58.3 23.6 0.0 1.840197 105112 107331 2312-2312 1.000000 65.4 ndhF_p5 ndhF 5 AGAAAAGGGGGAGAGATGGG TGGTTTGGTGAATCGGAAACA 20 21 55.0 42.9 58.4 58.3 0.0 0.0 1.742749 105112 107331 2372-2372 0.909091 62.5 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 58.1 57.9 0.0 0.0 0.251505 108401 109102 774-775 1.000000 78.9 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 58.1 57.9 0.0 0.0 0.251505 108401 109102 773-774 1.000000 78.9 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 58.1 57.8 0.0 0.0 0.312231 108401 109102 767-768 1.000000 78.4 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 58.1 57.8 0.0 0.0 0.312231 108401 109102 766-767 1.000000 78.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AGGGTTTTTCTGGGCAACAA GCTTGAGCACTGCTTCCTAA 20 20 45.0 50.0 58.1 58.2 0.0 4.7 0.313714 108401 109102 787-788 1.000000 78.4 rps19_p1 rps19 1 TGACCTCCATACTTCGATCGA TGAATCTTTCTATTCTGAATTCAG 21 24 47.6 29.2 58.1 52.9 15.2 26.6 10.188455 139286 139567 380-380 1.000000 40.0 rps19_p2 rps19 2 ACCTCCATACTTCGATCGAGA TGAATCTTTCTATTCTGAATTCAG 21 24 47.6 29.2 57.8 52.9 18.5 26.6 10.325715 139286 139567 378-378 1.000000 40.0 rps19_p3 rps19 3 CTGACCTCCATACTTCGATCG TGAATCTTTCTATTCTGAATTCAG 21 24 52.4 29.2 57.7 52.9 9.4 26.6 10.390883 139286 139567 381-381 1.000000 40.0 rps19_p4 rps19 4 TGACCTCCATACTTCGATCGA TTGAATCTTTCTATTCTGAATTCAG 21 25 47.6 28.0 58.1 53.7 15.2 26.6 10.413229 139286 139567 381-381 1.000000 40.0 rps19_p5 rps19 5 ACCTCCATACTTCGATCGAGA TTGAATCTTTCTATTCTGAATTCAG 21 25 47.6 28.0 57.8 53.7 18.5 26.6 10.550489 139286 139567 379-379 1.000000 40.0