primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	4585	4815	304-323	1.000000	82.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GGGCAATTTGGCGAAGAAAA	20	20	45.0	45.0	58.1	57.8	8.1	15.8	0.301643	4585	4815	778-821	1.000000	82.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GTGTTTCCTTGTTCCGGGAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.170464	4585	4815	688-688	0.500000	58.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GTGTTTCCTTGTTCCGGGAT	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	58.1	57.8	0.0	15.8	0.256682	4585	4815	1162-1162	0.500000	57.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTGTTTCCTTGTTCCGGGAT	TCGCTTTTTGAATCGAGGGT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.273275	4585	4815	1395-1395	0.500000	57.8
rpoC2_p1	rpoC2	1	GAGCCCTTATTGCTGCTGAT	GCCTGGGTCAGTGATTTGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.111846	13364	14938	2452-2452	0.500000	59.1
rpoC2_p2	rpoC2	2	TGACTTCGAGCCCTTATTGC	GCCTGGGTCAGTGATTTGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.114476	13364	14938	2459-2459	0.500000	59.1
rpoC2_p3	rpoC2	3	GAGCCCTTATTGCTGCTGAT	GATGCATTTCCTCTAGCCCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.118608	13364	14938	2924-2924	0.500000	59.1
rpoC2_p4	rpoC2	4	ACTTCGAGCCCTTATTGCTG	GCCTGGGTCAGTGATTTGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.120138	13364	14938	2457-2457	0.500000	59.0
rpoC2_p5	rpoC2	5	TGACTTCGAGCCCTTATTGC	GATGCATTTCCTCTAGCCCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.121238	13364	14938	2931-2931	0.500000	59.0
rpoC2-trnC-GCA_p1	rpoC2-trnC-GCA	1	GTTTTGGCAGGCCTACTCAT	TTGGTACCTGTTTGAGCCAC	20	20	50.0	50.0	58.2	58.0	4.0	14.4	0.184917	14939	16938	2248-2248	0.500000	58.5
rpoC2-trnC-GCA_p2	rpoC2-trnC-GCA	2	GTTTTGGCAGGCCTACTCAT	CAGAGCCTCTCGTGAGTCTA	20	20	50.0	55.0	58.2	58.0	4.0	13.1	0.191835	14939	16938	2642-2642	0.500000	58.5
rpoC2-trnC-GCA_p3	rpoC2-trnC-GCA	3	GTTTTGGCAGGCCTACTCAT	TACTCAGAGCCTCTCGTGAG	20	20	50.0	55.0	58.2	58.0	4.0	7.5	0.191835	14939	16938	2646-2646	0.500000	58.5
rpoC2-trnC-GCA_p4	rpoC2-trnC-GCA	4	GTTTTGGCAGGCCTACTCAT	TGAGTACTCAGAGCCTCTCG	20	20	50.0	55.0	58.2	58.0	4.0	21.7	0.191835	14939	16938	2650-2650	0.500000	58.5
rpoC2-trnC-GCA_p5	rpoC2-trnC-GCA	5	GTTTTGGCAGGCCTACTCAT	GAGTACTCAGAGCCTCTCGT	20	20	50.0	55.0	58.2	58.0	4.0	18.2	0.193257	14939	16938	2649-2649	0.500000	58.5
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGTGCTCTAACCGATTGAACT	CTGGTTCAAATCCAGCTCGG	21	20	47.6	55.0	58.0	58.9	0.0	0.0	1.936136	18191	18485	335-348	1.000000	70.6
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GGTGCTCTAACCGATTGAACT	GAGATGTCTACGCTGGTTCA	21	20	47.6	50.0	58.0	57.1	0.0	0.0	1.967508	18191	18485	347-360	1.000000	70.1
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGTGCTCTAACCGATTGAACT	TCTACGCTGGTTCAAATCCA	21	20	47.6	45.0	58.0	56.9	0.0	0.0	2.167354	18191	18485	341-354	1.000000	68.6
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GGTGCTCTAACCGATTGAACT	TGGTTCAAATCCAGCTCGG	21	19	47.6	52.6	58.0	57.8	0.0	0.0	2.274099	18191	18485	334-347	1.000000	67.9
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GGTGCTCTAACCGATTGAACT	GAGATGTCTACGCTGGTTCAA	21	21	47.6	47.6	58.0	57.8	0.0	0.0	2.258268	18191	18485	347-360	1.000000	67.8
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	TCTTGCTTGGGCTGCTTTAA	CCATGGCGTTACTCTACCAC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.070111	18570	18628	1004-1248	1.000000	82.8
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	TCTTGCTTGGGCTGCTTTAA	TGGCGTTACTCTACCACTGA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.092266	18570	18628	1001-1245	1.000000	82.7
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	GGACTTGAGTAAGAGTGGCG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.066398	18570	18628	559-559	0.500000	59.5
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	GGACTTGAGTAAGAGTGGCG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.088553	18570	18628	556-556	0.500000	59.3
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GGACTTGAGTAAGAGTGGCG	CCATTGGAACGGAACCAGAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.098044	18570	18628	992-992	0.500000	59.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TCGGAATCGAACCAATGACC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	11.5	0.0	0.023166	29063	29815	837-1076	1.000000	82.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TCGGAATCGAACCAATGACC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	11.5	0.0	0.087975	29063	29815	845-1084	1.000000	82.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	29063	29815	803-1042	1.000000	81.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	29063	29815	811-1050	1.000000	81.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TCGGAATCGAACCAATGACC	ATCCAATCAAGTCCGTAGCG	20	20	50.0	50.0	58.0	57.8	11.5	0.0	0.243610	29063	29815	860-1099	1.000000	80.9
accD-psaI_p1	accD-psaI	1	GACAGTACCCGACGGTTTAC	AATCGGTCATAACCACTGCC	20	20	55.0	50.0	58.0	58.0	13.9	0.0	0.055775	38476	39205	1585-1672	1.000000	89.0
accD-psaI_p2	accD-psaI	2	CTACATTGCGTTTACGGGGA	AATCGGTCATAACCACTGCC	20	20	50.0	50.0	58.0	58.0	4.3	0.0	0.051324	38476	39205	1633-1720	1.000000	88.8
accD-psaI_p3	accD-psaI	3	CCTACAACTGGCGGAGTTAC	AATCGGTCATAACCACTGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.044357	38476	39205	1701-1788	1.000000	88.4
accD-psaI_p4	accD-psaI	4	CCTACAACTGGCGGAGTTAC	AATAGACCCGCGATCCCATA	20	20	55.0	50.0	58.0	58.1	0.0	8.2	0.072949	38476	39205	1641-1728	1.000000	88.4
accD-psaI_p5	accD-psaI	5	CCTACAACTGGCGGAGTTAC	CGGTCATAACCACTGCCTAC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.070038	38476	39205	1698-1785	1.000000	88.3
psaI_p1	psaI	1	ACCGAGAACCCCTTTTTAGC	AATCGGTCATAACCACTGCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234128	39206	39310	1005-1005	0.500000	58.1
psaI_p2	psaI	2	ACCGAGAACCCCTTTTTAGC	CGGTCATAACCACTGCCTAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.259809	39206	39310	1002-1002	0.500000	57.9
psaI_p3	psaI	3	ACCGAGAACCCCTTTTTAGC	AATAGACCCGCGATCCCATA	20	20	50.0	50.0	57.8	58.1	0.0	8.2	0.262720	39206	39310	945-945	0.500000	57.9
psaI_p4	psaI	4	ACCGAGAACCCCTTTTTAGC	TCGGTCATAACCACTGCCTA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.340446	39206	39310	1003-1003	0.500000	57.3
psaI_p5	psaI	5	ACCGAGAACCCCTTTTTAGC	GGCCAATTCAGCAGCTTTTT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.370105	39206	39310	1033-1033	0.500000	57.0
psaI-ycf4_p1	psaI-ycf4	1	TCCGGCAATTACAATGGCTT	AATAGACCCGCGATCCCATA	20	20	45.0	50.0	58.2	58.1	0.0	8.2	0.220972	39311	39620	581-660	1.000000	84.4
psaI-ycf4_p2	psaI-ycf4	2	TCCGGCAATTACAATGGCTT	AATCGGTCATAACCACTGCC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.192380	39311	39620	641-720	1.000000	84.3
psaI-ycf4_p3	psaI-ycf4	3	TTCCGGCAATTACAATGGCT	AATCGGTCATAACCACTGCC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.192380	39311	39620	642-721	1.000000	84.3
psaI-ycf4_p4	psaI-ycf4	4	TCCGGCAATTACAATGGCTT	CGGTCATAACCACTGCCTAC	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.218061	39311	39620	638-717	1.000000	84.2
psaI-ycf4_p5	psaI-ycf4	5	TTCCGGCAATTACAATGGCT	CGGTCATAACCACTGCCTAC	20	20	45.0	55.0	58.2	58.1	0.0	0.0	0.218061	39311	39620	639-718	1.000000	84.2
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCGTGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	41416	41575	221-229	1.000000	84.9
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCGTGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	41416	41575	219-227	1.000000	84.9
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCGTGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	41416	41575	222-230	1.000000	84.9
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCGTGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	41416	41575	232-240	1.000000	84.7
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCGTGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	41416	41575	233-241	1.000000	84.6
psbB_p1	psbB	1	GGAATCATTGTGCTTCACGC	CGTGACCGGACTTCTTGAAT	20	20	50.0	50.0	58.1	57.9	0.0	9.5	0.180049	47369	48449	1776-2780	1.000000	87.4
psbB_p2	psbB	2	GGAATCATTGTGCTTCACGC	GTGCCTAGAATTACCACGGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.150297	47369	48449	2076-2076	0.500000	58.8
psbB_p3	psbB	3	CAGGAAGCCGAAAAGGAACT	GTGCCTAGAATTACCACGGG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.152975	47369	48449	1896-1896	0.500000	58.8
psbB_p4	psbB	4	GGAATCATTGTGCTTCACGC	AAAGAGCTATTGCGGTACCG	20	20	50.0	50.0	58.1	58.1	0.0	30.1	0.163830	47369	48449	2359-2359	0.500000	58.7
psbB_p5	psbB	5	CAGGAAGCCGAAAAGGAACT	AAAGAGCTATTGCGGTACCG	20	20	50.0	50.0	58.1	58.1	0.0	30.1	0.166508	47369	48449	2179-2179	0.500000	58.7
ndhD-rpl32_p1	ndhD-rpl32	1	TAAATGCACATTCCCCGCTT	TCCACCGGACAGTCAAAAAG	20	20	45.0	50.0	58.2	58.0	0.0	9.5	0.192200	83146	83515	1126-1126	0.500000	58.5
ndhD-rpl32_p2	ndhD-rpl32	2	GGCACGAGGATTTGAAGGAA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.2	58.0	0.0	9.5	0.216990	83146	83515	796-796	0.500000	58.3
ndhD-rpl32_p3	ndhD-rpl32	3	AGTGGCACGAGGATTTGAAG	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.2	58.0	12.7	9.5	0.224693	83146	83515	799-799	0.500000	58.2
ndhD-rpl32_p4	ndhD-rpl32	4	GAGTGGCACGAGGATTTGAA	TCCACCGGACAGTCAAAAAG	20	20	50.0	50.0	58.2	58.0	12.7	9.5	0.229796	83146	83515	800-800	0.500000	58.2
ndhD-rpl32_p5	ndhD-rpl32	5	TAAATGCACATTCCCCGCTT	AATCTGTTTCCACCGGACAG	20	20	45.0	50.0	58.2	57.8	0.0	9.5	0.338997	83146	83515	1134-1134	0.500000	57.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CTTTTTGACTGTCCGGTGGA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.162484	83672	84274	776-902	1.000000	85.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CTTTTTGACTGTCCGGTGGA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.223211	83672	84274	769-895	1.000000	84.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CTTTTTGACTGTCCGGTGGA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.234501	83672	84274	774-900	1.000000	84.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CTTTTTGACTGTCCGGTGGA	GCCCATAGAGAAGCCGTATG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.124261	83672	84274	914-2603	1.000000	82.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CTTTTTGACTGTCCGGTGGA	TTTGTGGCCCATAGAGAAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.166977	83672	84274	920-2609	1.000000	81.9