primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK_p1	matK	1	TCCTTACTACATACTACATCAACA	ACCCCCAAAATCCTCTACCT	24	20	33.3	50.0	54.6	57.6	0.0	0.0	7.815402	2064	3584	1681-1681	1.000000	40.0
matK_p2	matK	2	ACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	53.5	57.6	0.0	0.0	7.867308	2064	3584	1673-1673	1.000000	40.0
matK_p3	matK	3	ACATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	24	20	29.2	50.0	54.3	57.6	0.0	0.0	8.080774	2064	3584	1673-1673	1.000000	40.0
matK_p4	matK	4	ACTACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	26	20	30.8	50.0	56.2	57.6	0.0	0.0	8.140388	2064	3584	1676-1676	1.000000	40.0
matK_p5	matK	5	CCTTACTACATACTACATCAACA	ACCCCCAAAATCCTCTACCT	23	20	34.8	50.0	53.1	57.6	0.0	0.0	8.270202	2064	3584	1680-1680	1.000000	40.0
matK-trnK_p1	matK-trnK	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3585	4313	861-861	1.000000	58.8
matK-trnK_p2	matK-trnK	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3585	4313	860-860	1.000000	58.8
matK-trnK_p3	matK-trnK	3	AGAAGTGGTGTTGTTGAGATCT	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.3	58.2	0.0	17.1	2.864899	3585	4313	808-808	1.000000	57.2
matK-trnK_p4	matK-trnK	4	AGAAGTGGTGTTGTTGAGATCT	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.3	58.2	0.0	17.1	2.864899	3585	4313	807-807	1.000000	57.2
matK-trnK_p5	matK-trnK	5	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3585	4313	876-876	1.000000	57.0
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9376	10189	870-870	1.000000	78.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9376	10189	888-888	1.000000	76.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9376	10189	869-869	1.000000	72.4
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9376	10189	878-878	1.000000	72.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9376	10189	927-927	1.000000	71.7
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	0.0	0.0	2.573941	10280	10752	635-635	1.000000	59.7
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	0.0	0.0	2.785752	10280	10752	635-635	1.000000	58.0
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.1	55.6	0.0	0.0	3.315377	10280	10752	636-636	1.000000	53.7
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.9	55.6	0.0	0.0	3.324543	10280	10752	627-627	1.000000	53.7
petN-psbM_p5	petN-psbM	5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	21	20	47.6	40.0	58.0	55.6	0.0	0.0	3.423261	10280	10752	621-621	1.000000	52.9
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	25027	26204	1292-1314	1.000000	79.0
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	25027	26204	1348-1370	1.000000	78.4
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	25027	26204	1308-1330	1.000000	78.1
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	25027	26204	1293-1315	1.000000	77.9
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	25027	26204	1352-1374	1.000000	77.8
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.378951	31119	32356	1369-1378	1.000000	78.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCCATGAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.510157	31119	32356	1425-1434	1.000000	77.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TTCCATGAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.510157	31119	32356	1426-1435	1.000000	77.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.511907	31119	32356	1359-1368	1.000000	77.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.526423	31119	32356	1367-1376	1.000000	77.7
ycf3-trnS-GCU_p1	ycf3-trnS-GCU	1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	43996	44906	1021-1021	1.000000	70.5
ycf3-trnS-GCU_p2	ycf3-trnS-GCU	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	43996	44906	971-971	1.000000	70.2
ycf3-trnS-GCU_p3	ycf3-trnS-GCU	3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	21	20	47.6	55.0	58.0	57.8	8.2	0.0	1.260963	43996	44906	1009-1009	1.000000	70.0
ycf3-trnS-GCU_p4	ycf3-trnS-GCU	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	43996	44906	973-973	1.000000	69.7
ycf3-trnS-GCU_p5	ycf3-trnS-GCU	5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	21	20	47.6	50.0	58.0	57.6	8.2	0.0	1.437074	43996	44906	999-999	1.000000	68.6
accD-psaI_p1	accD-psaI	1	AAAAAGCGTTCTGGGTGAGT	AAAGAAGCCATTGCGATTGC	20	20	45.0	45.0	57.9	58.0	0.0	1.6	0.053212	58362	59088	865-865	1.000000	79.7
accD-psaI_p2	accD-psaI	2	AAAAGCGTTCTGGGTGAGTT	AAAGAAGCCATTGCGATTGC	20	20	45.0	45.0	57.9	58.0	0.0	1.6	0.053212	58362	59088	864-864	1.000000	79.7
accD-psaI_p3	accD-psaI	3	GCGGCCGAGTATTTATTCCA	AAAGAAGCCATTGCGATTGC	20	20	50.0	45.0	58.1	58.0	33.5	1.6	0.126732	58362	59088	924-924	1.000000	79.1
accD-psaI_p4	accD-psaI	4	AAAAAGCGTTCTGGGTGAGT	TTGCGATTGCCGGAAATACT	20	20	45.0	45.0	57.9	58.3	0.0	8.0	0.307550	58362	59088	855-855	1.000000	77.6
accD-psaI_p5	accD-psaI	5	AAAAGCGTTCTGGGTGAGTT	TTGCGATTGCCGGAAATACT	20	20	45.0	45.0	57.9	58.3	0.0	8.0	0.307550	58362	59088	854-854	1.000000	77.6
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.259329	64402	65652	1398-1398	1.000000	78.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.264322	64402	65652	1396-1396	1.000000	78.1
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.364337	64402	65652	1321-1321	1.000000	77.3
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.369331	64402	65652	1319-1319	1.000000	77.2
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.2	0.0	0.0	0.379015	64402	65652	1407-1407	1.000000	77.1
clpP_p1	clpP	1	ATTGATGCGAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	20	20	45.0	45.0	57.9	56.9	0.0	0.0	1.202341	69706	71746	2127-2128	1.000000	70.6
clpP_p2	clpP	2	CTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.262153	69706	71746	2154-2155	1.000000	70.1
clpP_p3	clpP	3	AACTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69706	71746	2156-2157	1.000000	70.1
clpP_p4	clpP	4	GAAACTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69706	71746	2158-2159	1.000000	70.1
clpP_p5	clpP	5	TATGAAACTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.270657	69706	71746	2161-2162	1.000000	70.0
petD_p1	petD	1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	76268	77459	1326-1326	1.000000	77.1
petD_p2	petD	2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	76268	77459	1325-1325	1.000000	77.1
petD_p3	petD	3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	76268	77459	1329-1329	1.000000	76.8
petD_p4	petD	4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	76268	77459	1328-1328	1.000000	76.8
petD_p5	petD	5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	20	20	45.0	50.0	57.7	58.4	0.0	0.0	0.692034	76268	77459	1329-1329	1.000000	74.5
rpl16_p1	rpl16	1	AAAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	19	24	52.6	29.2	59.3	56.2	0.0	0.0	8.071213	80920	82359	1602-1603	1.000000	40.1
rpl16_p2	rpl16	2	CCCGCACCCGCTATTTTT	TCGTTTCTTCTTTTTCTTTTGTGT	18	24	55.6	29.2	58.4	56.2	0.0	0.0	8.210086	80920	82359	1593-1594	1.000000	40.1
rpl16_p3	rpl16	3	AAAAAGAAACCCGCACCCG	CGTTTCTTCTTTTTCTTTTGTGTTCA	19	26	52.6	30.8	59.3	58.0	0.0	0.0	8.265232	80920	82359	1601-1602	1.000000	40.1
rpl16_p4	rpl16	4	AAAAGAAACCCGCACCCG	TCGTTTCTTCTTTTTCTTTTGTGT	18	24	55.6	29.2	58.6	56.2	0.0	0.0	8.369406	80920	82359	1601-1602	1.000000	40.1
rpl16_p5	rpl16	5	CCCGCACCCGCTATTTTT	CGTTTCTTCTTTTTCTTTTGTGTTCA	18	26	55.6	30.8	58.4	58.0	0.0	0.0	8.404105	80920	82359	1592-1593	1.000000	40.1
ycf1_p1	ycf1	1	TGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	20	20	40.0	50.0	55.2	58.1	12.5	21.9	2.908259	109959	110078	254-254	1.000000	57.4
ycf1_p2	ycf1	2	TTGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	21	20	38.1	50.0	56.0	58.1	12.5	21.9	3.100357	109959	110078	255-255	1.000000	55.8
ycf1_p3	ycf1	3	ATTGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	22	20	36.4	50.0	56.5	58.1	12.5	21.9	3.574933	109959	110078	256-256	1.000000	52.0
ycf1_p4	ycf1	4	TGCACAGAAACATCTGAACT	GTATGACCATCGAGGGGGTT	20	20	40.0	55.0	55.2	58.9	12.5	21.9	3.666998	109959	110078	255-255	1.000000	51.3
ycf1_p5	ycf1	5	TGCACAGAAACATCTGAACT	TTGTATGACCATCGAGGGGG	20	20	40.0	55.0	55.2	58.9	12.5	21.9	3.667856	109959	110078	257-257	1.000000	51.3
ndhI_p1	ndhI	1	TGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	22	20	36.4	50.0	55.8	57.6	0.0	0.0	4.577432	117724	118224	695-695	1.000000	43.5
ndhI_p2	ndhI	2	ACTTGTTCCCAACTTATTTCACTG	TAAAACAGGATTCTCGCCCC	24	20	37.5	50.0	57.6	57.6	0.0	0.0	4.804537	117724	118224	698-698	1.000000	41.7
ndhI_p3	ndhI	3	CTTGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	24	20	37.5	50.0	57.6	57.6	0.0	0.0	4.804537	117724	118224	697-697	1.000000	41.7
ndhI_p4	ndhI	4	TGTTCCCAACTTATTTCACTGT	AGGATTCTCGCCCCCTAATC	22	20	36.4	55.0	55.8	58.6	0.0	0.0	4.811031	117724	118224	689-689	1.000000	41.6
ndhI_p5	ndhI	5	TTGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	23	20	34.8	50.0	56.6	57.6	0.0	0.0	4.857112	117724	118224	696-696	1.000000	41.3
ndhD_p1	ndhD	1	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTCT	20	24	45.0	33.3	57.7	56.2	0.0	0.0	6.072487	120188	121708	1635-1635	1.000000	40.1
ndhD_p2	ndhD	2	GAAAACCCTACTTGAGCCCA	TCTTTTCATTAGTCAACCAACTCT	20	24	50.0	33.3	57.4	56.2	0.0	0.0	6.343964	120188	121708	1669-1669	1.000000	40.1
ndhD_p3	ndhD	3	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTCTT	20	25	45.0	32.0	57.7	56.9	0.0	0.0	6.425101	120188	121708	1635-1635	1.000000	40.1
ndhD_p4	ndhD	4	GCCAAAACCTGTGCTCAAAA	TTCTTTTCATTAGTCAACCAACTCT	20	25	45.0	32.0	57.7	56.9	0.0	0.0	6.425101	120188	121708	1636-1636	1.000000	40.1
ndhD_p5	ndhD	5	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTC	20	23	45.0	34.8	57.7	54.8	0.0	0.0	6.458266	120188	121708	1635-1635	1.000000	40.1
ccsA_p1	ccsA	1	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAA	20	20	50.0	45.0	57.4	56.2	0.0	0.0	2.359681	121980	122948	1180-1180	1.000000	61.2
ccsA_p2	ccsA	2	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAAA	20	21	50.0	42.9	57.4	57.0	0.0	0.0	2.613245	121980	122948	1180-1180	1.000000	59.2
ccsA_p3	ccsA	3	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAAAG	20	22	50.0	45.5	57.4	58.1	0.0	0.0	2.632121	121980	122948	1180-1180	1.000000	59.0
ccsA_p4	ccsA	4	TGACTTGATTCACACGGTTGT	GCGGCATGACATCTTCTAAA	21	20	42.9	45.0	58.1	56.2	0.0	0.0	2.869610	121980	122948	1183-1183	1.000000	57.1
ccsA_p5	ccsA	5	ACTTGACTTGATTCACACGGT	GCGGCATGACATCTTCTAAA	21	20	42.9	45.0	57.8	56.2	0.0	0.0	2.976386	121980	122948	1186-1186	1.000000	56.3
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	123143	124041	1031-1031	1.000000	77.1
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	123143	124041	1032-1032	1.000000	77.1
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	123143	124041	1014-1014	1.000000	76.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	123143	124041	1015-1015	1.000000	76.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TTAGAAGATGTCATGCCGCC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.411413	123143	124041	1095-1095	1.000000	76.8
rpl32-ndhF_p1	rpl32-ndhF	1	CCGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	20	20	50.0	50.0	57.6	58.0	0.0	17.5	0.397386	124207	125271	1238-1245	1.000000	77.4
rpl32-ndhF_p2	rpl32-ndhF	2	CCGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	124207	125271	1236-1243	1.000000	77.3
rpl32-ndhF_p3	rpl32-ndhF	3	CCGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	20	20	50.0	45.0	57.6	58.0	0.0	17.5	0.408676	124207	125271	1237-1244	1.000000	77.3
rpl32-ndhF_p4	rpl32-ndhF	4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	20	20	45.0	50.0	57.6	58.0	9.5	17.5	0.425347	124207	125271	1255-1262	1.000000	77.2
rpl32-ndhF_p5	rpl32-ndhF	5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	20	20	45.0	45.0	57.6	58.0	9.5	17.5	0.436636	124207	125271	1253-1260	1.000000	77.1