primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl14_p1	psaJ-rpl14	1	AGCAAAAGCAACAACTGGTG	ATTTTTGGTCCTGTAGCCCG	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.464223	58581	58772	321-568	1.000000	84.2
psaJ-rpl14_p2	psaJ-rpl14	2	AAGCAAAAGCAACAACTGGT	ATTTTTGGTCCTGTAGCCCG	20	20	40.0	50.0	56.9	58.2	0.0	0.0	1.223001	58581	58772	322-569	1.000000	78.1
psaJ-rpl14_p3	psaJ-rpl14	3	ACCAAGCAAAAGCAACAACT	ATTTTTGGTCCTGTAGCCCG	20	20	40.0	50.0	56.9	58.2	0.0	0.0	1.223001	58581	58772	325-572	1.000000	78.1
psaJ-rpl14_p4	psaJ-rpl14	4	AGCAAAAGCAACAACTGGTG	GTCCTGTAGCCCGTGAATTA	20	20	45.0	50.0	57.7	57.0	0.0	0.0	1.272105	58581	58772	314-561	1.000000	77.9
psaJ-rpl14_p5	psaJ-rpl14	5	AGCAAAAGCAACAACTGGTG	TCCTGTAGCCCGTGAATTAC	20	20	45.0	50.0	57.7	57.0	0.0	0.0	1.272105	58581	58772	313-560	1.000000	77.9
rpl14-rpl16_p1	rpl14-rpl16	1	TCTACGAACACCTTTACGCG	TGTATATGGAGGGTTTGCGC	20	20	50.0	50.0	58.0	58.0	3.5	0.0	0.070445	59142	59619	1019-1019	0.500000	59.4
rpl14-rpl16_p2	rpl14-rpl16	2	TCTACGAACACCTTTACGCG	CGTATGGGTTCTGGGAAAGG	20	20	50.0	55.0	58.0	58.0	3.5	0.0	0.070625	59142	59619	856-856	0.500000	59.4
rpl14-rpl16_p3	rpl14-rpl16	3	TCTACGAACACCTTTACGCG	GTTTGCGCTTCAAGCTACAG	20	20	50.0	50.0	58.0	58.0	3.5	9.7	0.076476	59142	59619	1007-1007	0.500000	59.4
rpl14-rpl16_p4	rpl14-rpl16	4	GCCAGTATTATCGGCGACAT	TGTATATGGAGGGTTTGCGC	20	20	50.0	50.0	57.9	58.0	13.8	0.0	0.118493	59142	59619	851-851	0.500000	59.1
rpl14-rpl16_p5	rpl14-rpl16	5	GCCAGTATTATCGGCGACAT	CGTATGGGTTCTGGGAAAGG	20	20	50.0	55.0	57.9	58.0	13.8	0.0	0.118673	59142	59619	688-688	0.500000	59.1
rpl16-atpF_p1	rpl16-atpF	1	CCTTTCATACGACCACGATGA	TCTTTATTGCTCGTTTTACAGA	21	22	47.6	31.8	57.8	53.8	0.0	0.0	7.346061	60028	61813	1877-2081	1.000000	52.0
rpl16-atpF_p2	rpl16-atpF	2	TCCTTTCATACGACCACGATG	TCTTTATTGCTCGTTTTACAGA	21	22	47.6	31.8	57.8	53.8	0.0	0.0	7.346061	60028	61813	1878-2082	1.000000	52.0
rpl16-atpF_p3	rpl16-atpF	3	CCTTTCATACGACCACGATGA	TCTTTATTGCTCGTTTTACAGAA	21	23	47.6	30.4	57.8	54.6	0.0	0.0	7.550105	60028	61813	1877-2081	1.000000	52.0
rpl16-atpF_p4	rpl16-atpF	4	CCTTTCATACGACCACGATGA	TTCTTTATTGCTCGTTTTACAGA	21	23	47.6	30.4	57.8	54.6	0.0	0.0	7.550105	60028	61813	1878-2082	1.000000	52.0
rpl16-atpF_p5	rpl16-atpF	5	TCCTTTCATACGACCACGATG	TCTTTATTGCTCGTTTTACAGAA	21	23	47.6	30.4	57.8	54.6	0.0	0.0	7.550105	60028	61813	1878-2082	1.000000	52.0
atpB-rpl5_p1	atpB-rpl5	1	ACATGCTTTTACCCCTTGCA	AACGTTTTCCCAGAACAGCT	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.061025	66506	67642	2075-2075	0.500000	59.5
atpB-rpl5_p2	atpB-rpl5	2	AGACTGTAGGACTTGCACCT	AACGTTTTCCCAGAACAGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.069687	66506	67642	1971-1971	0.500000	59.4
atpB-rpl5_p3	atpB-rpl5	3	GCATGATCCCACGATTCACT	AACGTTTTCCCAGAACAGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.093741	66506	67642	2199-2199	0.500000	59.3
atpB-rpl5_p4	atpB-rpl5	4	CTGCATGATCCCACGATTCA	AACGTTTTCCCAGAACAGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.095114	66506	67642	2201-2201	0.500000	59.2
atpB-rpl5_p5	atpB-rpl5	5	ACTGAATCTCCCTTCCCGAA	AACGTTTTCCCAGAACAGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.100161	66506	67642	1920-1920	0.500000	59.2
rpl5-rps7_p1	rpl5-rps7	1	GCTTCACCTACACCACGATT	GTGCTAGAGCTATCGCCATC	20	20	50.0	55.0	57.9	58.0	0.0	2.1	0.105332	68186	68784	969-969	0.500000	59.2
rpl5-rps7_p2	rpl5-rps7	2	GCTTCACCTACACCACGATT	TTGAAGTCAAACCACGTCGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.107699	68186	68784	1038-1038	0.500000	59.1
rpl5-rps7_p3	rpl5-rps7	3	GCTTCACCTACACCACGATT	TATGGCTCGTAAAGGTGCAC	20	20	50.0	50.0	57.9	58.0	0.0	8.8	0.113304	68186	68784	1256-1256	0.500000	59.1
rpl5-rps7_p4	rpl5-rps7	4	GCTTCACCTACACCACGATT	TGAATTCACGTCGTCCAGAC	20	20	50.0	50.0	57.9	57.9	0.0	3.4	0.155088	68186	68784	804-804	0.500000	58.8
rpl5-rps7_p5	rpl5-rps7	5	GCTTCACCTACACCACGATT	CGTGTTGAAGTCAAACCACG	20	20	50.0	50.0	57.9	57.9	0.0	17.3	0.180294	68186	68784	1042-1042	0.500000	58.6
rps12-atpH_p1	rps12-atpH	1	ACGTACTCGAGCGACTTTTC	ATTGGTCCTGGTATGGGTCA	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.059112	71792	73988	2554-2554	0.500000	59.5
rps12-atpH_p2	rps12-atpH	2	ACGTACTCGAGCGACTTTTC	GAGGAAAGATGGCAGAGTGG	20	20	50.0	55.0	58.0	58.0	13.6	0.0	0.074451	71792	73988	2803-2803	0.500000	59.4
rps12-atpH_p3	rps12-atpH	3	ACGTACTCGAGCGACTTTTC	TTATGCGGTTGAAGGGATCG	20	20	50.0	50.0	58.0	58.1	13.6	0.0	0.090817	71792	73988	2519-2519	0.500000	59.3
rps12-atpH_p4	rps12-atpH	4	ACGTACTCGAGCGACTTTTC	GGTTATGCGGTTGAAGGGAT	20	20	50.0	50.0	58.0	57.9	13.6	0.0	0.091704	71792	73988	2521-2521	0.500000	59.3
rps12-atpH_p5	rps12-atpH	5	ACTCGACCTCCACGAACTAA	ATTGGTCCTGGTATGGGTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.118600	71792	73988	2646-2646	0.500000	59.1
rps18-ycf4_p1	rps18-ycf4	1	TTTTGGTGGAATTACCGGCA	CAAGCTGCTGAACTTGCAAA	20	20	45.0	45.0	58.0	57.8	0.0	1.4	0.216070	82254	83712	1651-1651	0.500000	58.3
rps18-ycf4_p2	rps18-ycf4	2	TTTTGGTGGAATTACCGGCA	AAACAAGCTGCTGAACTTGC	20	20	45.0	45.0	58.0	57.8	0.0	1.4	0.217589	82254	83712	1654-1654	0.500000	58.3
rps18-ycf4_p3	rps18-ycf4	3	TTTTGGTGGAATTACCGGCA	TTTTTCGCTGGGGTTTTCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.220130	82254	83712	1845-1845	0.500000	58.2
rps18-ycf4_p4	rps18-ycf4	4	TTTTGGTGGAATTACCGGCA	CAACCCTCAACGTACCCTTT	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.268790	82254	83712	1748-1748	0.500000	57.8
rps18-ycf4_p5	rps18-ycf4	5	CGTTAGTTTTCCCAGCCACT	CAAGCTGCTGAACTTGCAAA	20	20	50.0	45.0	58.1	57.8	0.0	1.4	0.320659	82254	83712	2188-2188	0.500000	57.4
ycf4-rpoA_p1	ycf4-rpoA	1	TTTGCAAGTTCAGCAGCTTG	AAGTTGCTTGGTCATGGGAG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.297728	84256	85426	2088-2088	0.500000	57.6
ycf4-rpoA_p2	ycf4-rpoA	2	TTTGCAAGTTCAGCAGCTTG	AGAAGTTGCTTGGTCATGGG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.297728	84256	85426	2090-2090	0.500000	57.6
ycf4-rpoA_p3	ycf4-rpoA	3	GCAAGTTCAGCAGCTTGTTT	AAGTTGCTTGGTCATGGGAG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.299246	84256	85426	2085-2085	0.500000	57.6
ycf4-rpoA_p4	ycf4-rpoA	4	GCAAGTTCAGCAGCTTGTTT	AGAAGTTGCTTGGTCATGGG	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.299246	84256	85426	2087-2087	0.500000	57.6
ycf4-rpoA_p5	ycf4-rpoA	5	AGGAAAACCCCAGCGAAAAA	AAGTTGCTTGGTCATGGGAG	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.301788	84256	85426	1894-1894	0.500000	57.6
rpoA_p1	rpoA	1	AGCTTGTTCTGGGAAAACGT	TTCGAAAAGCGTCGTAACCA	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.202406	85427	87193	2864-2864	0.500000	58.4
rpoA_p2	rpoA	2	TTTCCAAGAAGAGCTGGCTG	TTCGAAAAGCGTCGTAACCA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.248032	85427	87193	2822-2822	0.500000	58.0
rpoA_p3	rpoA	3	GGCTTTCGCGGAAAATCTTC	TTCGAAAAGCGTCGTAACCA	20	20	50.0	45.0	58.1	58.1	6.1	0.0	0.253278	85427	87193	2887-2887	0.500000	58.0
rpoA_p4	rpoA	4	GATGGCTGGGAAAACTACGT	TTCGAAAAGCGTCGTAACCA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.259048	85427	87193	2650-2650	0.500000	57.9
rpoA_p5	rpoA	5	AGCTTGTTCTGGGAAAACGT	AAAAGCGTCGTAACCAGGAG	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.260776	85427	87193	2860-2860	0.500000	57.9
rpl32-rpoBb_p1	rpl32-rpoBb	1	AGACGCACAAGGGCTTAAAA	TATTTGATGGTCGCACTGGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061298	90327	91022	1771-1771	0.500000	59.5
rpl32-rpoBb_p2	rpl32-rpoBb	2	TTTTAGACGCACAAGGGCTT	TATTTGATGGTCGCACTGGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061298	90327	91022	1775-1775	0.500000	59.5
rpl32-rpoBb_p3	rpl32-rpoBb	3	TTTTTAGACGCACAAGGGCT	TATTTGATGGTCGCACTGGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061298	90327	91022	1776-1776	0.500000	59.5
rpl32-rpoBb_p4	rpl32-rpoBb	4	AGACGCACAAGGGCTTAAAA	ATCAACCCATTACGACTGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.062727	90327	91022	1741-1741	0.500000	59.5
rpl32-rpoBb_p5	rpl32-rpoBb	5	TTTTAGACGCACAAGGGCTT	ATCAACCCATTACGACTGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.062727	90327	91022	1745-1745	0.500000	59.5
rpoBb_p1	rpoBb	1	AAAAAGTGTGCTGTCGACCT	GCCAGTAACCGAGTTGTAGG	20	20	45.0	55.0	58.0	58.0	14.1	0.2	0.044357	91023	93254	2686-2686	0.500000	59.6
rpoBb_p2	rpoBb	2	TTGATCCCACCACTTTCTGC	GCCAGTAACCGAGTTGTAGG	20	20	50.0	55.0	58.1	58.0	0.0	0.2	0.096271	91023	93254	2718-2718	0.500000	59.2
rpoBb_p3	rpoBb	3	AAAAAGTGTGCTGTCGACCT	TTTTTAACCAGCCAGCAGGT	20	20	45.0	45.0	58.0	57.9	14.1	0.1	0.119041	91023	93254	2707-2707	0.500000	59.0
rpoBb_p4	rpoBb	4	AAAAAGTGTGCTGTCGACCT	CCGATTTGGCTCTTCTTGGA	20	20	45.0	50.0	58.0	57.9	14.1	0.0	0.152247	91023	93254	2831-2831	0.500000	58.8
rpoBb_p5	rpoBb	5	AAAAAGTGTGCTGTCGACCT	TCCGATTTGGCTCTTCTTGG	20	20	45.0	50.0	58.0	57.9	14.1	0.0	0.152247	91023	93254	2832-2832	0.500000	58.8
rpoBb-atpE_p1	rpoBb-atpE	1	CCCCCAATACTAGAGGCAGA	TTCCCAGAACCAGCCATCTA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137739	93255	93948	1431-1431	0.500000	58.9
rpoBb-atpE_p2	rpoBb-atpE	2	CCCCCAATACTAGAGGCAGA	AGCTTGTTCTGGGAAAACGT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.155936	93255	93948	1605-1605	0.500000	58.8
rpoBb-atpE_p3	rpoBb-atpE	3	CCCCCAATACTAGAGGCAGA	TTTTCCCAGAACCAGCCATC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.179798	93255	93948	1433-1433	0.500000	58.6
rpoBb-atpE_p4	rpoBb-atpE	4	GCTTGAACCCAGTCTCCTTC	TTCCCAGAACCAGCCATCTA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.219793	93255	93948	1392-1392	0.500000	58.2
rpoBb-atpE_p5	rpoBb-atpE	5	GCTTGAACCCAGTCTCCTTC	AGCTTGTTCTGGGAAAACGT	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.237989	93255	93948	1566-1566	0.500000	58.1
atpE-rpoC2_p1	atpE-rpoC2	1	AATCCGCTTCTGCTTGTTCA	CGAGCAGTTACTAGAAGCCC	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.315133	94357	94989	1484-1484	0.500000	57.5
atpE-rpoC2_p2	atpE-rpoC2	2	AATCCGCTTCTGCTTGTTCA	GAGCAGTTACTAGAAGCCCG	20	20	45.0	55.0	58.1	57.8	0.0	0.0	0.315133	94357	94989	1483-1483	0.500000	57.5
atpE-rpoC2_p3	atpE-rpoC2	3	TAATCTCCGCTGACTGTCCA	CGAGCAGTTACTAGAAGCCC	20	20	50.0	55.0	58.2	57.8	5.2	0.0	0.364024	94357	94989	1265-1265	0.500000	57.1
atpE-rpoC2_p4	atpE-rpoC2	4	TAATCTCCGCTGACTGTCCA	GAGCAGTTACTAGAAGCCCG	20	20	50.0	55.0	58.2	57.8	5.2	0.0	0.364024	94357	94989	1264-1264	0.500000	57.1
atpE-rpoC2_p5	atpE-rpoC2	5	GGGAAAACTTACGGACTGGG	GAGCAGTTACTAGAAGCCCG	20	20	55.0	55.0	58.2	57.8	0.0	0.0	0.393995	94357	94989	1912-1912	0.500000	56.8
rpoC2-petL_p1	rpoC2-petL	1	AGAAATGTTTCCTCGAGCCC	GTTGTGTGGGGGCAGAATAA	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.197471	102817	103621	1784-1784	0.500000	58.4
rpoC2-petL_p2	rpoC2-petL	2	AGAAATGTTTCCTCGAGCCC	TGTGTGGGGGCAGAATAAAC	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.197471	102817	103621	1782-1782	0.500000	58.4
rpoC2-petL_p3	rpoC2-petL	3	AACCTCGCATCGAAACAAGT	TGTGTGGGGGCAGAATAAAC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.203175	102817	103621	1813-1813	0.500000	58.4
rpoC2-petL_p4	rpoC2-petL	4	ACCTCGCATCGAAACAAGTT	GTTGTGTGGGGGCAGAATAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.203175	102817	103621	1814-1814	0.500000	58.4
rpoC2-petL_p5	rpoC2-petL	5	ACCTCGCATCGAAACAAGTT	TGTGTGGGGGCAGAATAAAC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.203175	102817	103621	1812-1812	0.500000	58.4