primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS(gcu)-trnD(guc)_p1	trnS(gcu)-trnD(guc)	1	GGGTTCGAATCCCTGTTTCT	AGACTCGAACTCGCAACTTC	20	20	50.0	50.0	57.5	57.9	17.2	0.0	0.544957	3046	3105	145-153	1.000000	87.4
trnS(gcu)-trnD(guc)_p2	trnS(gcu)-trnD(guc)	2	GGGTTCGAATCCCTGTTTCT	GAGACTCGAACTCGCAACTT	20	20	50.0	50.0	57.5	57.9	17.2	4.0	0.544957	3046	3105	146-154	1.000000	87.4
trnS(gcu)-trnD(guc)_p3	trnS(gcu)-trnD(guc)	3	GGGTTCGAATCCCTGTTTCT	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	57.5	58.6	17.2	7.1	1.042691	3046	3105	113-121	1.000000	83.7
trnS(gcu)-trnD(guc)_p4	trnS(gcu)-trnD(guc)	4	GGGTTCGAATCCCTGTTTCT	GACTGACGAGACTCGAACTC	20	20	50.0	55.0	57.5	57.5	17.2	30.6	1.005639	3046	3105	153-161	1.000000	83.5
trnS(gcu)-trnD(guc)_p5	trnS(gcu)-trnD(guc)	5	GGGTTCGAATCCCTGTTTCT	GGACTGACGAGACTCGAACT	20	20	50.0	55.0	57.5	58.8	17.2	30.6	1.333241	3046	3105	154-162	1.000000	80.9
rpl20_p1	rpl20	1	AAAAGCTGCAGCAAAACGTT	TTAATGTTACAGGAGCGGCC	20	20	40.0	50.0	58.0	58.0	38.2	0.0	0.041432	8174	8518	1276-1276	0.111111	44.1
rpl20_p2	rpl20	2	AAAAAGACAGGCGCAGGTAA	TTAATGTTACAGGAGCGGCC	20	20	45.0	50.0	58.0	58.0	10.8	0.0	0.057021	8174	8518	1254-1254	0.111111	44.0
rpl20_p3	rpl20	3	AAAAGCTGCAGCAAAACGTT	AGTCTACCATGGACTGCTGT	20	20	40.0	50.0	58.0	58.1	38.2	21.5	0.064575	8174	8518	1213-1213	0.111111	43.9
rpl20_p4	rpl20	4	AAAAAGACAGGCGCAGGTAA	AGTCTACCATGGACTGCTGT	20	20	45.0	50.0	58.0	58.1	10.8	21.5	0.080163	8174	8518	1191-1191	0.111111	43.8
rpl20_p5	rpl20	5	CAGTTGATCAAGGGGCTGTT	TTAATGTTACAGGAGCGGCC	20	20	50.0	50.0	58.1	58.0	8.8	0.0	0.117540	8174	8518	1689-1689	0.111111	43.5
ycf89_p1	ycf89	1	AAAATGTTGGCCCAACTTGC	CATTGCGGAAAATTCCCCAC	20	20	45.0	50.0	58.0	58.0	26.0	6.6	0.042817	9044	10069	2153-2153	0.111111	44.1
ycf89_p2	ycf89	2	AAAATGTTGGCCCAACTTGC	TCTCGTACGACTTGCATGTG	20	20	45.0	50.0	58.0	58.0	26.0	15.3	0.056877	9044	10069	1877-1877	0.111111	44.0
ycf89_p3	ycf89	3	AAAATGTTGGCCCAACTTGC	TATTGCTCCGTCAGGCTTTC	20	20	45.0	50.0	58.0	58.0	26.0	0.0	0.058241	9044	10069	2176-2176	0.111111	44.0
ycf89_p4	ycf89	4	AAAATGTTGGCCCAACTTGC	AGTTGACTTGTCCAACCACC	20	20	45.0	50.0	58.0	57.9	26.0	0.0	0.086848	9044	10069	2359-2359	0.111111	43.7
ycf89_p5	ycf89	5	AAAATGTTGGCCCAACTTGC	GTTGACTTGTCCAACCACCT	20	20	45.0	50.0	58.0	57.9	26.0	0.0	0.086848	9044	10069	2358-2358	0.111111	43.7
rns-trnI(gau)_p1	rns-trnI(gau)	1	GACTGGGGTGAAGTCGTAAC	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	57.9	57.9	0.0	6.7	0.224425	11741	11805	199-202	1.000000	87.0
rns-trnI(gau)_p2	rns-trnI(gau)	2	GGGGTGAAGTCGTAACAAGG	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.2	57.9	0.0	6.7	0.343845	11741	11805	195-198	1.000000	86.1
rns-trnI(gau)_p3	rns-trnI(gau)	3	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.3	57.9	0.0	6.7	0.430050	11741	11805	176-179	1.000000	85.5
rns-trnI(gau)_p4	rns-trnI(gau)	4	TGACTGGGGTGAAGTCGTAA	CTGGGCCATTCTGGATTTGA	20	20	50.0	50.0	58.3	57.9	0.0	6.7	0.441339	11741	11805	200-203	1.000000	85.3
rns-trnI(gau)_p5	rns-trnI(gau)	5	AGGTAACCGTACTGGAAGGT	CTGGGCCATTCTGGATTTGA	20	20	50.0	50.0	57.7	57.9	10.2	6.7	0.464166	11741	11805	178-181	1.000000	85.2
ycf35_p1	ycf35	1	CCTCCTGGGAAGATAGCTCA	AACCTTCGGAAACAGTTGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.146882	15637	16014	1115-1115	0.111111	43.3
ycf35_p2	ycf35	2	ACCTCCTGGGAAGATAGCTC	AACCTTCGGAAACAGTTGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.148386	15637	16014	1116-1116	0.111111	43.3
ycf35_p3	ycf35	3	GACCTCCTGGGAAGATAGCT	AACCTTCGGAAACAGTTGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.148386	15637	16014	1117-1117	0.111111	43.3
ycf35_p4	ycf35	4	CCTCCTGGGAAGATAGCTCA	TGGAGCCCGTCAACTAGTTA	20	20	55.0	50.0	57.9	58.1	0.0	8.0	0.166613	15637	16014	1655-1655	0.111111	43.1
ycf35_p5	ycf35	5	ACCTCCTGGGAAGATAGCTC	TGGAGCCCGTCAACTAGTTA	20	20	55.0	50.0	57.9	58.1	0.0	8.0	0.168117	15637	16014	1656-1656	0.111111	43.1
rps16_p1	rps16	1	TCGTTCCCATTCCAAGCAAA	TGGAGCCCGTCAACTAGTTA	20	20	45.0	50.0	58.0	58.1	0.0	8.0	0.084219	16155	16391	912-912	0.111111	43.8
rps16_p2	rps16	2	TTCGTTCCCATTCCAAGCAA	TGGAGCCCGTCAACTAGTTA	20	20	45.0	50.0	58.0	58.1	0.0	8.0	0.084219	16155	16391	913-913	0.111111	43.8
rps16_p3	rps16	3	TCGTTCCCATTCCAAGCAAA	AAAAGATCGTCCAGGTCAGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.112311	16155	16391	1155-1155	0.111111	43.5
rps16_p4	rps16	4	TTCGTTCCCATTCCAAGCAA	AAAAGATCGTCCAGGTCAGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.112311	16155	16391	1156-1156	0.111111	43.5
rps16_p5	rps16	5	GGGCATGATTGTTCCCAGAA	TGGAGCCCGTCAACTAGTTA	20	20	50.0	50.0	58.1	58.1	0.0	8.0	0.221209	16155	16391	957-957	0.111111	42.7
syfB_p1	syfB	1	GCTGACCTGGACGATCTTTT	ACTTTGGAAACTCGCGATGT	20	20	50.0	45.0	57.9	58.1	0.0	10.4	0.221652	17531	19645	2866-2866	0.111111	42.7
syfB_p2	syfB	2	AGTGAAGATTTGGGCGAGTG	ACTTTGGAAACTCGCGATGT	20	20	50.0	45.0	58.2	58.1	0.0	10.4	0.307534	17531	19645	2713-2713	0.111111	42.0
syfB_p3	syfB	3	GCTGACCTGGACGATCTTTT	AGGTACTTTGGAAACTCGCG	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.308599	17531	19645	2870-2870	0.111111	42.0
syfB_p4	syfB	4	GTGAAGATTTGGGCGAGTGA	ACTTTGGAAACTCGCGATGT	20	20	50.0	45.0	58.2	58.1	0.0	10.4	0.312636	17531	19645	2712-2712	0.111111	41.9
syfB_p5	syfB	5	TGAAGATTTGGGCGAGTGAC	ACTTTGGAAACTCGCGATGT	20	20	50.0	45.0	58.2	58.1	0.0	10.4	0.312636	17531	19645	2711-2711	0.111111	41.9
ccsA_p1	ccsA	1	CCGATTGCTCTACCACTGAG	CAATGTGTTCGTGCATGTCC	20	20	55.0	50.0	57.8	58.0	0.0	5.7	0.248145	44818	45756	1785-1870	1.000000	89.7
ccsA_p2	ccsA	2	AAGATTGCTTGTACGCAGCT	CAATGTGTTCGTGCATGTCC	20	20	45.0	50.0	58.2	58.0	15.7	5.7	0.218725	44818	45756	1309-1309	0.111111	42.7
ccsA_p3	ccsA	3	AAGATTGCTTGTACGCAGCT	CGGTTGTACGCAATGTGTTC	20	20	45.0	50.0	58.2	58.1	15.7	4.3	0.241279	44818	45756	1319-1319	0.111111	42.5
ccsA_p4	ccsA	4	AAGATTGCTTGTACGCAGCT	GGTTGTACGCAATGTGTTCG	20	20	45.0	50.0	58.2	58.1	15.7	4.3	0.241279	44818	45756	1318-1318	0.111111	42.5
ccsA_p5	ccsA	5	AAGATTGCTTGTACGCAGCT	TGTTCGTGCATGTCCTACTG	20	20	45.0	50.0	58.2	57.9	15.7	5.7	0.265335	44818	45756	1304-1304	0.111111	42.3
ccs1_p1	ccs1	1	CCACTCACGACCCATGTAAG	CAGAATCACGACTACGCCAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.019346	47493	48767	2363-2363	0.111111	44.3
ccs1_p2	ccs1	2	CACGACCCATGTAAGATGCA	CAGAATCACGACTACGCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031965	47493	48767	2358-2358	0.111111	44.2
ccs1_p3	ccs1	3	TACAGGCTCACGGATACCAT	CAGAATCACGACTACGCCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091341	47493	48767	2171-2171	0.111111	43.7
ccs1_p4	ccs1	4	CCATAATGAAACGCCTTCGC	CAGAATCACGACTACGCCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.114524	47493	48767	2012-2012	0.111111	43.5
ccs1_p5	ccs1	5	GAAGTGCATACCGATAGCGT	CAGAATCACGACTACGCCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.144865	47493	48767	2249-2249	0.111111	43.3
trnI(gau)-rns_p1	trnI(gau)-rns	1	CTGGGCCATTCTGGATTTGA	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	57.9	6.7	0.0	0.224425	59013	59077	199-202	1.000000	87.0
trnI(gau)-rns_p2	trnI(gau)-rns	2	CTGGGCCATTCTGGATTTGA	GGGGTGAAGTCGTAACAAGG	20	20	50.0	55.0	57.9	58.2	6.7	0.0	0.343845	59013	59077	195-198	1.000000	86.1
trnI(gau)-rns_p3	trnI(gau)-rns	3	CTGGGCCATTCTGGATTTGA	GTAACCGTACTGGAAGGTGC	20	20	50.0	55.0	57.9	58.3	6.7	0.0	0.430050	59013	59077	176-179	1.000000	85.5
trnI(gau)-rns_p4	trnI(gau)-rns	4	CTGGGCCATTCTGGATTTGA	TGACTGGGGTGAAGTCGTAA	20	20	50.0	50.0	57.9	58.3	6.7	0.0	0.441339	59013	59077	200-203	1.000000	85.3
trnI(gau)-rns_p5	trnI(gau)-rns	5	CTGGGCCATTCTGGATTTGA	AGGTAACCGTACTGGAAGGT	20	20	50.0	50.0	57.9	57.7	6.7	10.2	0.464166	59013	59077	178-181	1.000000	85.2
ycf89_p1	ycf89	1	CATTGCGGAAAATTCCCCAC	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.0	58.1	6.6	12.8	0.060647	60749	61774	2299-2299	0.111111	44.0
ycf89_p2	ycf89	2	TCTCGTACGACTTGCATGTG	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.0	58.1	15.3	12.8	0.074707	60749	61774	2023-2023	0.111111	43.8
ycf89_p3	ycf89	3	TATTGCTCCGTCAGGCTTTC	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.0	58.1	0.0	12.8	0.076071	60749	61774	2322-2322	0.111111	43.8
ycf89_p4	ycf89	4	AGTTGACTTGTCCAACCACC	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	57.9	58.1	0.0	12.8	0.104678	60749	61774	2505-2505	0.111111	43.6
ycf89_p5	ycf89	5	GTTGACTTGTCCAACCACCT	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	57.9	58.1	0.0	12.8	0.104678	60749	61774	2504-2504	0.111111	43.6
ycf89-trnP(ugg)_p1	ycf89-trnP(ugg)	1	TTAATGTTACAGGAGCGGCC	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.0	58.1	0.0	12.8	0.084576	61775	62072	963-963	0.111111	43.8
ycf89-trnP(ugg)_p2	ycf89-trnP(ugg)	2	AGTCTACCATGGACTGCTGT	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.1	58.1	21.5	12.8	0.107719	61775	62072	900-900	0.111111	43.6
ycf89-trnP(ugg)_p3	ycf89-trnP(ugg)	3	TTAATGTTACAGGAGCGGCC	GACGTTGTCCCAACTTCTGA	20	20	50.0	50.0	58.0	57.8	0.0	5.7	0.266980	61775	62072	962-962	0.111111	42.3
ycf89-trnP(ugg)_p4	ycf89-trnP(ugg)	4	AGTCTACCATGGACTGCTGT	GACGTTGTCCCAACTTCTGA	20	20	50.0	50.0	58.1	57.8	21.5	5.7	0.290122	61775	62072	899-899	0.111111	42.1
ycf89-trnP(ugg)_p5	ycf89-trnP(ugg)	5	TTACCAGGCTGTCCTTCACT	TGACGTTGTCCCAACTTCTG	20	20	50.0	50.0	58.3	58.1	14.4	12.8	0.322309	61775	62072	1435-1435	0.111111	41.9
trnP(ugg)-ycf45_p1	trnP(ugg)-ycf45	1	TTAATGTTACAGGAGCGGCC	TTTCGCGTCAAGAGTTTCCA	20	20	50.0	45.0	58.0	58.1	0.0	5.8	0.090003	62147	62629	1350-1350	0.111111	43.7
trnP(ugg)-ycf45_p2	trnP(ugg)-ycf45	2	AGTCTACCATGGACTGCTGT	TTTCGCGTCAAGAGTTTCCA	20	20	50.0	45.0	58.1	58.1	21.5	5.8	0.113145	62147	62629	1287-1287	0.111111	43.5
trnP(ugg)-ycf45_p3	trnP(ugg)-ycf45	3	TTAATGTTACAGGAGCGGCC	CTTGCAGCTCGTACAATTGC	20	20	50.0	50.0	58.0	58.1	0.0	5.1	0.145360	62147	62629	1795-1795	0.111111	43.3
trnP(ugg)-ycf45_p4	trnP(ugg)-ycf45	4	AGTCTACCATGGACTGCTGT	CTTGCAGCTCGTACAATTGC	20	20	50.0	50.0	58.1	58.1	21.5	5.1	0.168503	62147	62629	1732-1732	0.111111	43.1
trnP(ugg)-ycf45_p5	trnP(ugg)-ycf45	5	CCAAGCTGAGCTATATCCCG	TTTCGCGTCAAGAGTTTCCA	20	20	55.0	45.0	57.6	58.1	7.7	5.8	0.434130	62147	62629	775-775	0.111111	41.0
rpoC2_p1	rpoC2	1	ACACAATGATGGCCGTTCAA	GTCGGGCCAAGTATACCAAG	20	20	45.0	55.0	58.4	58.1	11.8	4.4	0.435538	69371	69490	823-823	0.111111	41.0
rpoC2_p2	rpoC2	2	TTGACACAATGATGGCCGTT	GTCGGGCCAAGTATACCAAG	20	20	45.0	55.0	58.4	58.1	0.0	4.4	0.435538	69371	69490	826-826	0.111111	41.0
rpoC2_p3	rpoC2	3	ACACAATGATGGCCGTTCAA	CTATGGATTTTGTCGGGCCA	20	20	45.0	50.0	58.4	57.9	11.8	4.4	0.436338	69371	69490	834-834	0.111111	41.0
rpoC2_p4	rpoC2	4	TTGACACAATGATGGCCGTT	CTATGGATTTTGTCGGGCCA	20	20	45.0	50.0	58.4	57.9	0.0	4.4	0.436338	69371	69490	837-837	0.111111	41.0
rpoC2_p5	rpoC2	5	ACACAATGATGGCCGTTCAA	ACTATGGATTTTGTCGGGCC	20	20	45.0	50.0	58.4	57.9	11.8	0.0	0.437786	69371	69490	835-835	0.111111	40.9
rpoB_p1	rpoB	1	TTAGCTGCGCCAGTTGTTTA	AGAATTGGCCATGTTCTCGC	20	20	45.0	50.0	58.1	58.9	4.2	21.9	1.016390	77059	77178	1163-1163	0.111111	36.3
rpoB_p2	rpoB	2	TTAGCTGCGCCAGTTGTTTA	CCATGTTCTCGCGAATTCATG	20	21	45.0	47.6	58.1	58.0	4.2	25.9	1.136427	77059	77178	1155-1155	0.111111	35.4
rpoB_p3	rpoB	3	CTTTTTAGCTGCGCCAGTTG	AGAATTGGCCATGTTCTCGC	20	20	50.0	50.0	58.3	58.9	4.2	21.9	1.211991	77059	77178	1167-1167	0.111111	34.7
rpoB_p4	rpoB	4	TTTCACTTTTTAGCTGCGCC	AGAATTGGCCATGTTCTCGC	20	20	45.0	50.0	57.6	58.9	4.2	21.9	1.317647	77059	77178	1172-1172	0.111111	33.9
rpoB_p5	rpoB	5	CTTTTTAGCTGCGCCAGTTG	CCATGTTCTCGCGAATTCATG	20	21	50.0	47.6	58.3	58.0	4.2	25.9	1.332028	77059	77178	1159-1159	0.111111	33.8
ycf41_p1	ycf41	1	CGATTTGGATTTCGCGATGG	GGTTTCTTTGAGTGGGGTCA	20	20	50.0	50.0	58.0	57.6	10.9	0.0	0.381130	99813	100133	834-860	0.333333	54.3
ycf41_p2	ycf41	2	CGATTTGGATTTCGCGATGG	TGGTTTCTTTGAGTGGGGTC	20	20	50.0	50.0	58.0	57.6	10.9	0.0	0.381130	99813	100133	835-861	0.333333	54.3
ycf41_p3	ycf41	3	CGATTTGGATTTCGCGATGG	AATTAGCCGGTCAAACTGCT	20	20	50.0	45.0	58.0	57.8	10.9	1.9	0.223181	99813	100133	931-931	0.111111	42.7
ycf41_p4	ycf41	4	CGATTTGGATTTCGCGATGG	CAATTAGCCGGTCAAACTGC	20	20	50.0	50.0	58.0	57.7	10.9	1.9	0.285380	99813	100133	932-932	0.111111	42.2
ycf41_p5	ycf41	5	GGATTTCGCGATGGATCACT	AATTAGCCGGTCAAACTGCT	20	20	50.0	45.0	58.1	57.8	10.9	1.9	0.329293	99813	100133	925-925	0.111111	41.8