primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ycf4	1	ATGAGAGTGACTTTGGGAGT	CGTTTGGGTGTTGGAGAAGA	20	20	45.0	50.0	55.8	58.0	0.0	0.0	2.271319	8222	8767	648-649	1.000000	62.7
ycf4_p2	ycf4	2	ATGAGAGTGACTTTGGGAGT	TCGTTTGGGTGTTGGAGAAG	20	20	45.0	50.0	55.8	58.0	0.0	0.0	2.271319	8222	8767	649-650	1.000000	62.7
ycf4_p3	ycf4	3	ATGAGAGTGACTTTGGGAGT	TTCGTTTGGGTGTTGGAGAA	20	20	45.0	45.0	55.8	57.6	0.0	0.0	2.654864	8222	8767	650-651	1.000000	59.6
ycf4_p4	ycf4	4	ATGAGAGTGACTTTGGGAGT	ATTCGTTTGGGTGTTGGAGA	20	20	45.0	45.0	55.8	57.4	0.0	0.0	2.882644	8222	8767	651-652	1.000000	57.8
ycf4_p5	ycf4	5	GTGTTTGGTCACTCTTTCAACT	CGTTTGGGTGTTGGAGAAGA	22	20	40.9	50.0	57.0	58.0	0.0	0.0	3.003326	8222	8767	617-618	1.000000	56.9
petA-psbJ_p1	petA-psbJ	1	GCATCTGTCATTTTGGCACA	TCCGGATTAGGATCATCCCT	20	20	45.0	50.0	57.3	57.0	0.0	0.0	1.751333	11401	12163	871-871	1.000000	66.9
petA-psbJ_p2	petA-psbJ	2	AGTGCAATTGGTCGAGATGA	TCCGGATTAGGATCATCCCT	20	20	45.0	50.0	57.2	57.0	7.7	0.0	1.790896	11401	12163	815-815	1.000000	66.7
petA-psbJ_p3	petA-psbJ	3	GCATCTGTCATTTTGGCACA	CGGAAGGATCCCTCTTTGGT	20	20	45.0	55.0	57.3	59.1	0.0	8.6	1.816203	11401	12163	956-956	1.000000	66.3
petA-psbJ_p4	petA-psbJ	4	AGTGCAATTGGTCGAGATGA	CGGAAGGATCCCTCTTTGGT	20	20	45.0	55.0	57.2	59.1	7.7	8.6	1.855767	11401	12163	900-900	1.000000	66.0
petA-psbJ_p5	petA-psbJ	5	GCATCTGTCATTTTGGCACA	GGAAGGATCCCTCTTTGGTT	20	20	45.0	50.0	57.3	56.8	0.0	8.6	1.933824	11401	12163	955-955	1.000000	65.4
petG-trnW-CCA_p1	petG-trnW-CCA	1	CGACGTGGTGATCAATTGGA	CTCCAAAACCTGATGCCGTA	20	20	50.0	50.0	58.3	57.9	5.4	0.0	0.387145	14495	14613	188-188	1.000000	81.2
petG-trnW-CCA_p2	petG-trnW-CCA	2	CGACGTGGTGATCAATTGGA	TCCAAAACCTGATGCCGTAG	20	20	50.0	50.0	58.3	57.9	5.4	0.0	0.387145	14495	14613	187-187	1.000000	81.2
petG-trnW-CCA_p3	petG-trnW-CCA	3	CGACGTGGTGATCAATTGGA	AGCGCTCTTAGTTCAGTTGG	20	20	50.0	50.0	58.3	57.9	5.4	2.6	0.365250	14495	14613	221-221	1.000000	80.7
petG-trnW-CCA_p4	petG-trnW-CCA	4	CGACGTGGTGATCAATTGGA	GTTCAGTTGGGTAGAACGCA	20	20	50.0	50.0	58.3	58.1	5.4	0.0	0.405498	14495	14613	211-211	1.000000	80.5
petG-trnW-CCA_p5	petG-trnW-CCA	5	CGACGTGGTGATCAATTGGA	TTCAGTTGGGTAGAACGCAG	20	20	50.0	50.0	58.3	57.8	5.4	0.0	0.443013	14495	14613	210-210	1.000000	80.3
clpP-accD_p1	clpP-accD	1	ATTATAGCGGATACTCGTAAG	CATGGTTCCACGGGCTATTT	21	20	38.1	50.0	52.2	58.2	0.0	0.0	6.995946	18854	19778	1022-1130	1.000000	42.3
clpP-accD_p2	clpP-accD	2	ATTATAGCGGATACTCGTAAG	TTGATACCATGGTTCCACGG	21	20	38.1	50.0	52.2	57.6	0.0	25.4	7.170281	18854	19778	1029-1137	1.000000	42.3
clpP-accD_p3	clpP-accD	3	ATTATAGCGGATACTCGTAAG	ATGGTTCCACGGGCTATTTT	21	20	38.1	45.0	52.2	57.5	0.0	0.0	7.283758	18854	19778	1021-1129	1.000000	42.3
clpP-accD_p4	clpP-accD	4	TATTATAGCGGATACTCGTAAG	CATGGTTCCACGGGCTATTT	22	20	36.4	50.0	52.3	58.2	0.0	0.0	7.893247	18854	19778	1023-1131	1.000000	42.3
clpP-accD_p5	clpP-accD	5	ATTATAGCGGATACTCGTAAG	TGGTTCCACGGGCTATTTTA	21	20	38.1	45.0	52.2	56.8	0.0	0.0	7.934871	18854	19778	1020-1128	1.000000	42.3
accD_p1	accD	1	TTGACCGATGCCTGTTTGAA	TGCAGCACGTCTAAAAGAGA	20	20	45.0	45.0	58.0	57.2	0.0	0.0	0.838093	20745	20864	802-1018	1.000000	82.5
accD_p2	accD	2	ACCTACTACCGAGCCCATAC	TGCAGCACGTCTAAAAGAGA	20	20	55.0	45.0	58.0	57.2	0.0	0.0	0.819821	20745	20864	877-1093	1.000000	82.3
accD_p3	accD	3	CACCTACTACCGAGCCCATA	TGCAGCACGTCTAAAAGAGA	20	20	55.0	45.0	58.0	57.2	0.0	0.0	0.821237	20745	20864	878-1094	1.000000	82.3
accD_p4	accD	4	GCCAGAAGCACATACGAGAA	TGCAGCACGTCTAAAAGAGA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.822503	20745	20864	949-1165	1.000000	82.0
accD_p5	accD	5	ACCTACTACCGAGCCCATAC	ACTTTTTGGGTGGACTTCCA	20	20	55.0	45.0	58.0	57.2	0.0	0.0	0.846099	20745	20864	911-1127	1.000000	82.0
chlB-trnQ-UUG_p1	chlB-trnQ-UUG	1	GAGCAGGACCAGCATACATC	TGGTCCTGTTATTGCGAAGG	20	20	55.0	50.0	58.1	57.9	11.9	0.0	0.231628	26273	26455	260-261	1.000000	80.3
chlB-trnQ-UUG_p2	chlB-trnQ-UUG	2	GAGCAGGACCAGCATACATC	GGTCCTGTTATTGCGAAGGT	20	20	55.0	50.0	58.1	57.9	11.9	0.0	0.233103	26273	26455	259-260	1.000000	80.3
chlB-trnQ-UUG_p3	chlB-trnQ-UUG	3	AGAGGAGCATGCATTATGGC	TGGTCCTGTTATTGCGAAGG	20	20	50.0	50.0	58.1	57.9	22.0	0.0	0.200960	26273	26455	324-325	1.000000	80.1
chlB-trnQ-UUG_p4	chlB-trnQ-UUG	4	AGAGGAGCATGCATTATGGC	GGTCCTGTTATTGCGAAGGT	20	20	50.0	50.0	58.1	57.9	22.0	0.0	0.202435	26273	26455	323-324	1.000000	80.1
chlB-trnQ-UUG_p5	chlB-trnQ-UUG	5	AGCTACTCGTAGGGTTCCAA	TGGTCCTGTTATTGCGAAGG	20	20	50.0	50.0	57.8	57.9	6.3	0.0	0.334912	26273	26455	283-284	1.000000	79.3
psbK-psbI_p1	psbK-psbI	1	CAAGCTGCCGTAAGTTTTCG	TCACTGTTCTTTACGCCCAG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.213346	27047	27669	761-780	1.000000	79.8
psbK-psbI_p2	psbK-psbI	2	AGCTGCCGTAAGTTTTCGAT	TCACTGTTCTTTACGCCCAG	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.258580	27047	27669	759-778	1.000000	79.5
psbK-psbI_p3	psbK-psbI	3	AAGCTGCCGTAAGTTTTCGA	TCACTGTTCTTTACGCCCAG	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.290120	27047	27669	760-779	1.000000	79.2
psbK-psbI_p4	psbK-psbI	4	GAGGCTTATGCGATTTCCGA	TCACTGTTCTTTACGCCCAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.297953	27047	27669	842-861	1.000000	79.0
psbK-psbI_p5	psbK-psbI	5	CAAGCTGCCGTAAGTTTTCG	CTGTTCTTTACGCCCAGGAT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.452780	27047	27669	758-777	1.000000	77.9
trnS-GCU_p1	trnS-GCU	1	CTGGGCGTAAAGAACAGTGA	TCGCAAATCGCAAATGTTGG	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.468005	27821	27908	191-191	1.000000	77.5
trnS-GCU_p2	trnS-GCU	2	CTGGGCGTAAAGAACAGTGA	GGATTCTCGCAAATCGCAAA	20	20	50.0	45.0	57.8	57.5	0.0	4.3	0.706584	27821	27908	197-197	1.000000	75.6
trnS-GCU_p3	trnS-GCU	3	ATCCTGGGCGTAAAGAACAG	TCGCAAATCGCAAATGTTGG	20	20	50.0	45.0	57.6	58.3	0.0	0.0	0.707439	27821	27908	194-194	1.000000	75.6
trnS-GCU_p4	trnS-GCU	4	CTGGGCGTAAAGAACAGTGA	TGGATTCTCGCAAATCGCAA	20	20	50.0	45.0	57.8	58.6	0.0	4.3	0.715873	27821	27908	198-198	1.000000	75.5
trnS-GCU_p5	trnS-GCU	5	CTGGGCGTAAAGAACAGTGA	TTGGATTCTCGCAAATCGCA	20	20	50.0	45.0	57.8	58.6	0.0	4.3	0.715873	27821	27908	199-199	1.000000	75.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	48792	49795	1099-1109	1.000000	79.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	TGAACACCGGGTTTTGTAGG	20	20	50.0	50.0	57.8	58.0	0.0	6.3	0.199741	48792	49795	1148-1158	1.000000	79.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	CCTGAACACCGGGTTTTGTA	20	20	50.0	50.0	57.8	58.0	0.0	6.3	0.199741	48792	49795	1150-1160	1.000000	79.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	48792	49795	1078-1088	1.000000	79.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	48792	49795	1075-1085	1.000000	79.3
psaA-ycf3_p1	psaA-ycf3	1	ATAGGATCCCTCTCCACCAC	TGGTTTGATCAAGCTGCTGA	20	20	55.0	45.0	57.6	57.7	8.6	19.9	0.682474	59602	60199	753-767	1.000000	76.7
psaA-ycf3_p2	psaA-ycf3	2	ATAGGATCCCTCTCCACCAC	CGGAGGCTTGGTTTGATCAA	20	20	55.0	50.0	57.6	58.5	8.6	3.4	0.873715	59602	60199	761-775	1.000000	75.2
psaA-ycf3_p3	psaA-ycf3	3	ATAGGATCCCTCTCCACCAC	TGGAAACAAGCTATAGCGCT	20	20	55.0	45.0	57.6	57.3	8.6	17.3	1.098749	59602	60199	729-743	1.000000	73.5
psaA-ycf3_p4	psaA-ycf3	4	AAATGACCAGGTTTAGCCCA	TGGTTTGATCAAGCTGCTGA	20	20	45.0	45.0	57.0	57.7	0.0	19.9	1.265874	59602	60199	792-806	1.000000	72.0
psaA-ycf3_p5	psaA-ycf3	5	TGACCAGGTTTAGCCCATTT	TGGTTTGATCAAGCTGCTGA	20	20	45.0	45.0	57.0	57.7	0.0	19.9	1.265874	59602	60199	789-803	1.000000	72.0
rps4-trnQ-UUG_p1	rps4-trnQ-UUG	1	TTTTAAACGAGGTCCGCGAT	CCTTCGCAATAACAGGACCA	20	20	45.0	50.0	57.9	57.9	22.6	0.0	0.191116	63592	64142	616-638	1.000000	82.8
rps4-trnQ-UUG_p2	rps4-trnQ-UUG	2	TTTTAAACGAGGTCCGCGAT	ACCTTCGCAATAACAGGACC	20	20	45.0	50.0	57.9	57.9	22.6	0.0	0.192591	63592	64142	617-639	1.000000	82.8
rps4-trnQ-UUG_p3	rps4-trnQ-UUG	3	TTTTTAAACGAGGTCCGCGA	CCTTCGCAATAACAGGACCA	20	20	45.0	50.0	58.1	57.9	22.6	0.0	0.244683	63592	64142	617-639	1.000000	82.4
rps4-trnQ-UUG_p4	rps4-trnQ-UUG	4	TTTCTTGACAAGTCGCTCCC	CCTTCGCAATAACAGGACCA	20	20	50.0	50.0	58.1	57.9	14.7	0.0	0.228997	63592	64142	694-716	1.000000	82.0
rps4-trnQ-UUG_p5	rps4-trnQ-UUG	5	TTTCTTGACAAGTCGCTCCC	ACCTTCGCAATAACAGGACC	20	20	50.0	50.0	58.1	57.9	14.7	0.0	0.230472	63592	64142	695-717	1.000000	82.0
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TCTGGTCGGTTTTTCATCGG	GTAACGCTCACAACTTCCCT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.361985	77005	77291	443-443	1.000000	79.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TCTGGTCGGTTTTTCATCGG	TGGAAGTTATGCACGAACGT	20	20	50.0	45.0	58.2	57.8	0.0	7.5	0.354500	77005	77291	461-461	1.000000	79.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TCTGGTCGGTTTTTCATCGG	GTTATGCACGAACGTAACGC	20	20	50.0	50.0	58.2	58.2	0.0	13.5	0.419156	77005	77291	456-456	1.000000	78.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TCTGGTCGGTTTTTCATCGG	TTATGCACGAACGTAACGCT	20	20	50.0	45.0	58.2	58.3	0.0	13.5	0.496143	77005	77291	455-455	1.000000	78.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTTGGAC	TGGAAGTTATGCACGAACGT	20	20	50.0	45.0	58.4	57.8	0.4	7.5	0.537012	77005	77291	404-404	1.000000	78.1
chlN-ycf1_p1	chlN-ycf1	1	GAAGACTTGGATCGGACCAC	CCAATACGACATCATGGCCA	20	20	55.0	50.0	58.0	58.0	0.0	8.6	0.045605	81407	82513	999-1379	1.000000	86.0
chlN-ycf1_p2	chlN-ycf1	2	ACTTGGATCGGACCACCTTA	CCAATACGACATCATGGCCA	20	20	50.0	50.0	58.1	58.0	1.3	8.6	0.087081	81407	82513	995-1375	1.000000	85.6
chlN-ycf1_p3	chlN-ycf1	3	TTGGATCGGACCACCTTAGT	CCAATACGACATCATGGCCA	20	20	50.0	50.0	58.1	58.0	1.3	8.6	0.087081	81407	82513	993-1373	1.000000	85.6
chlN-ycf1_p4	chlN-ycf1	4	AGAAGACTTGGATCGGACCA	CCAATACGACATCATGGCCA	20	20	50.0	50.0	58.1	58.0	0.0	8.6	0.095481	81407	82513	1000-1380	1.000000	85.6
chlN-ycf1_p5	chlN-ycf1	5	CTTGGATCGGACCACCTTAG	CCAATACGACATCATGGCCA	20	20	55.0	50.0	57.4	58.0	1.3	8.6	0.638111	81407	82513	994-1374	1.000000	81.2
ycf1_p1	ycf1	1	ACCAAGGGACCGAAAATAGC	TTCTTGGAAAACCAGGGAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.128901	88808	88957	748-1294	1.000000	90.5
ycf1_p2	ycf1	2	ACCAAGGGACCGAAAATAGC	TCCCTTTGCCAATATCGGTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.175612	88808	88957	833-1379	1.000000	89.6
ycf1_p3	ycf1	3	ACCAAGGGACCGAAAATAGC	GTCAATGTCTCCCTTTGCCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.214577	88808	88957	842-1388	1.000000	89.2
ycf1_p4	ycf1	4	ACCAAGGGACCGAAAATAGC	CGGTCATTAAAGTACCCCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.180246	88808	88957	997-1543	1.000000	88.8
ycf1_p5	ycf1	5	ACCAAGGGACCGAAAATAGC	ACAGTTTGATTCGATCCGCA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.217583	88808	88957	963-1509	1.000000	88.6
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	ACAACGACGCGATTATCAGG	GTAGCTGTACTGGAAGGTGC	20	20	50.0	55.0	58.4	58.0	15.9	0.0	0.454976	111811	113217	1248-1611	1.000000	85.4
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	ACAACGACGCGATTATCAGG	GACCGGAGTGAAGTCGTAAC	20	20	50.0	55.0	58.4	58.0	15.9	9.5	0.468950	111811	113217	1271-1634	1.000000	85.2
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	CACAACGACGCGATTATCAG	GTAGCTGTACTGGAAGGTGC	20	20	50.0	55.0	57.4	58.0	15.9	0.0	0.622514	111811	113217	1249-1612	1.000000	84.1
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	CACAACGACGCGATTATCAG	GACCGGAGTGAAGTCGTAAC	20	20	50.0	55.0	57.4	58.0	15.9	9.5	0.636487	111811	113217	1272-1635	1.000000	83.9
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	ACAACGACGCGATTATCAGG	TGACCGGAGTGAAGTCGTAA	20	20	50.0	50.0	58.4	58.4	15.9	9.5	0.835382	111811	113217	1272-1635	1.000000	82.3
rrn16-rps12_p1	rrn16-rps12	1	ACTTCCCGTTTGACTTGCAT	TGGCAACTTTACGTAAGGCA	20	20	45.0	45.0	58.0	57.7	0.0	16.5	0.276434	114731	116296	1687-1758	1.000000	79.0
rrn16-rps12_p2	rrn16-rps12	2	AAACACCACTTCCCGTTTGA	TGGCAACTTTACGTAAGGCA	20	20	45.0	45.0	57.9	57.7	0.0	16.5	0.389467	114731	116296	1694-1765	1.000000	78.1
rrn16-rps12_p3	rrn16-rps12	3	ACTTCCCGTTTGACTTGCAT	AATCGCACTCTGGCAACTTT	20	20	45.0	45.0	58.0	58.4	0.0	0.0	0.409536	114731	116296	1697-1768	1.000000	78.0
rrn16-rps12_p4	rrn16-rps12	4	ACTTCCCGTTTGACTTGCAT	ATCGCACTCTGGCAACTTTA	20	20	45.0	45.0	58.0	57.5	0.0	0.0	0.491439	114731	116296	1696-1767	1.000000	77.3
rrn16-rps12_p5	rrn16-rps12	5	ACTTCCCGTTTGACTTGCAT	TAATCGCACTCTGGCAACTT	20	20	45.0	45.0	58.0	57.5	0.0	0.0	0.491439	114731	116296	1698-1769	1.000000	77.3
ycf2_p1	ycf2	1	TCCATCCGGGATCTGATGAA	TTGGGTTTTCTCAAGGTCCG	20	20	50.0	50.0	57.9	58.0	6.5	0.0	0.125980	127292	127411	899-1088	1.000000	84.8
ycf2_p2	ycf2	2	TCCATCCGGGATCTGATGAA	TCTCAGATAACCAAGGCCCA	20	20	50.0	50.0	57.9	58.0	6.5	0.0	0.136225	127292	127411	849-1038	1.000000	84.8
ycf2_p3	ycf2	3	TCCATCCGGGATCTGATGAA	TATCAACGTCGCTTGCTCAA	20	20	50.0	45.0	57.9	57.9	6.5	0.0	0.174756	127292	127411	934-1123	1.000000	84.3
ycf2_p4	ycf2	4	TCCATCCGGGATCTGATGAA	AAGTTGGGGAAGTCATTCGG	20	20	50.0	50.0	57.9	57.8	6.5	0.0	0.302012	127292	127411	827-1016	1.000000	83.5
ycf2_p5	ycf2	5	TCCATCCGGGATCTGATGAA	GGTTTTCTCAAGGTCCGACA	20	20	50.0	50.0	57.9	57.8	6.5	0.0	0.351483	127292	127411	896-1085	1.000000	83.0