primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH (GUG)-psbA_p1	trnH (GUG)-psbA	1	CAATCCACTGCCTTCATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	417	468-473	1.000000	78.5
trnH (GUG)-psbA_p2	trnH (GUG)-psbA	2	AATCCACTGCCTTCATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	417	467-472	1.000000	78.4
trnH (GUG)-psbA_p3	trnH (GUG)-psbA	3	ACAATCCACTGCCTTCATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	417	469-474	1.000000	78.4
trnH (GUG)-psbA_p4	trnH (GUG)-psbA	4	CAATCCACTGCCTTCATCCA	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.529871	78	417	469-474	1.000000	78.0
trnH (GUG)-psbA_p5	trnH (GUG)-psbA	5	AATCCACTGCCTTCATCCAC	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	78	417	468-473	1.000000	78.0
psbA-rbcL_p1	psbA-rbcL	1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.673162	1480	2556	1163-1163	1.000000	75.4
psbA-rbcL_p2	psbA-rbcL	2	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.703856	1480	2556	1192-1192	1.000000	75.1
psbA-rbcL_p3	psbA-rbcL	3	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.711287	1480	2556	1160-1160	1.000000	75.1
psbA-rbcL_p4	psbA-rbcL	4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.718766	1480	2556	1158-1158	1.000000	75.0
psbA-rbcL_p5	psbA-rbcL	5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	20	20	50.0	45.0	57.3	57.9	0.0	6.6	0.757962	1480	2556	1170-1170	1.000000	74.7
matK-trnK (UUU)_p1	matK-trnK (UUU)	1	CCATGATCATGAGCAAGTGC	TATGCTGTGATTCGCTCGTC	20	20	50.0	50.0	57.2	58.2	32.7	0.0	0.936131	9990	10720	938-946	1.000000	73.4
matK-trnK (UUU)_p2	matK-trnK (UUU)	2	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	9990	10720	855-862	1.000000	73.4
matK-trnK (UUU)_p3	matK-trnK (UUU)	3	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.2	58.2	32.7	17.1	0.944732	9990	10720	854-861	1.000000	73.4
matK-trnK (UUU)_p4	matK-trnK (UUU)	4	CCATGATCATGAGCAAGTGC	TTATGCTGTGATTCGCTCGT	20	20	50.0	45.0	57.2	57.7	32.7	0.0	1.069989	9990	10720	939-947	1.000000	72.4
matK-trnK (UUU)_p5	matK-trnK (UUU)	5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.2	57.5	32.7	0.0	1.237069	9990	10720	868-875	1.000000	71.0
ORF189_p1	ORF189	1	TGCTTTCTACCACAGCGTTT	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	11775	12344	993-1325	1.000000	86.3
ORF189_p2	ORF189	2	TTGCTTTCTACCACAGCGTT	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	11775	12344	994-1326	1.000000	86.3
ORF189_p3	ORF189	3	AGCGAACATCAATTGCAACG	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.100332	11775	12344	1031-1363	1.000000	86.1
ORF189_p4	ORF189	4	GCGAACATCAATTGCAACGA	GTCCCGGTAATCGAAGGTTC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.105682	11775	12344	1030-1362	1.000000	86.1
ORF189_p5	ORF189	5	GATCCCTTTATCACGCTGCA	GTCCCGGTAATCGAAGGTTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.114535	11775	12344	1498-1823	1.000000	85.0
ORF189-trnQ (UUG)_p1	ORF189-trnQ (UUG)	1	CTCGGGGCTTATCGTTTTCA	CGGGTCAAATTCCTAGCACA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.126492	12345	12530	1110-1538	1.000000	88.6
ORF189-trnQ (UUG)_p2	ORF189-trnQ (UUG)	2	TTGTGAGTGAGCCACCTTTC	GTCCCGGTAATCGAAGGTTC	20	20	50.0	55.0	58.0	58.1	2.2	0.0	0.100775	12345	12530	618-949	1.000000	88.1
ORF189-trnQ (UUG)_p3	ORF189-trnQ (UUG)	3	GAATGCCCCATGCCAAAATC	GTCCCGGTAATCGAAGGTTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.110426	12345	12530	760-1085	1.000000	87.9
ORF189-trnQ (UUG)_p4	ORF189-trnQ (UUG)	4	CTCGGGGCTTATCGTTTTCA	GTCCCGGTAATCGAAGGTTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.086636	12345	12530	787-1112	1.000000	87.8
ORF189-trnQ (UUG)_p5	ORF189-trnQ (UUG)	5	CTCGGGGCTTATCGTTTTCA	GTTTTGGTCCCGGTAATCGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.116222	12345	12530	793-1118	1.000000	87.5
trnQ (UUG)-psbK_p1	trnQ (UUG)-psbK	1	GAACCTTCGATTACCGGGAC	CGGGCAATTTGGCAAAGAAA	20	20	55.0	45.0	58.1	58.1	6.3	22.6	0.191772	12603	13115	650-753	1.000000	84.3
trnQ (UUG)-psbK_p2	trnQ (UUG)-psbK	2	TCGATTACCGGGACCAAAAC	CGGGCAATTTGGCAAAGAAA	20	20	50.0	45.0	57.9	58.1	6.3	22.6	0.221358	12603	13115	644-747	1.000000	84.1
trnQ (UUG)-psbK_p3	trnQ (UUG)-psbK	3	CTTCGATTACCGGGACCAAA	CGGGCAATTTGGCAAAGAAA	20	20	50.0	45.0	57.6	58.1	6.3	22.6	0.513640	12603	13115	646-749	1.000000	81.7
trnQ (UUG)-psbK_p4	trnQ (UUG)-psbK	4	GATTACCGGGACCAAAACCC	CGGGCAATTTGGCAAAGAAA	20	20	55.0	45.0	58.5	58.1	6.3	22.6	0.656822	12603	13115	642-745	1.000000	80.6
trnQ (UUG)-psbK_p5	trnQ (UUG)-psbK	5	TTCGATTACCGGGACCAAAA	CGGGCAATTTGGCAAAGAAA	20	20	45.0	45.0	57.1	58.1	6.3	22.6	0.978887	12603	13115	645-748	1.000000	78.0
trnS (GCU)-trnG (UCC)_p1	trnS (GCU)-trnG (UCC)	1	ACGGAAAGAGAGGGATTCGA	ACCGAACGACCCCTTAACTA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.227992	14055	14774	880-880	1.000000	79.1
trnS (GCU)-trnG (UCC)_p2	trnS (GCU)-trnG (UCC)	2	ACGAAAACGGAAAGAGAGGG	ACCGAACGACCCCTTAACTA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.241963	14055	14774	886-886	1.000000	79.0
trnS (GCU)-trnG (UCC)_p3	trnS (GCU)-trnG (UCC)	3	ACGGAAAGAGAGGGATTCGA	AAACCGAACGACCCCTTAAC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.268436	14055	14774	882-882	1.000000	78.8
trnS (GCU)-trnG (UCC)_p4	trnS (GCU)-trnG (UCC)	4	ACGAAAACGGAAAGAGAGGG	AAACCGAACGACCCCTTAAC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.282407	14055	14774	888-888	1.000000	78.6
trnS (GCU)-trnG (UCC)_p5	trnS (GCU)-trnG (UCC)	5	AGTCATCGACGAAAACGGAA	ACCGAACGACCCCTTAACTA	20	20	45.0	50.0	57.6	58.1	0.0	0.0	0.503876	14055	14774	894-894	1.000000	76.9
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GGTTGTCGCATTAGCCCTTT	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.944401	18744	19168	537-538	1.000000	73.3
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	ATTTATGGCCTGGTTGTCGC	20	20	50.0	50.0	57.9	58.9	0.0	3.3	1.015335	18744	19168	548-549	1.000000	72.8
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GTTGTCGCATTAGCCCTTTT	20	20	50.0	45.0	57.9	57.0	0.0	0.0	1.127970	18744	19168	536-537	1.000000	71.9
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GCGGAGGGGAAAATACGAG	20	19	50.0	57.9	57.9	58.0	0.0	0.0	1.130561	18744	19168	608-609	1.000000	71.7
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GGTTGTCGCATTAGCCCTT	20	19	50.0	52.6	57.9	58.1	0.0	0.0	1.250060	18744	19168	537-538	1.000000	70.9
rpoC2-rpoC1_p1	rpoC2-rpoC1	1	TGTGAAAGACCAGATTGGCC	GCGAAATCGAAGAGGCCATA	20	20	50.0	50.0	58.1	58.1	0.0	5.0	0.224435	26301	26465	247-252	1.000000	80.8
rpoC2-rpoC1_p2	rpoC2-rpoC1	2	TGTGAAAGACCAGATTGGCC	GAGGCCATACAGGGGTTTTC	20	20	50.0	55.0	58.1	58.2	0.0	5.0	0.326332	26301	26465	236-241	1.000000	80.1
rpoC2-rpoC1_p3	rpoC2-rpoC1	3	TGTGAAAGACCAGATTGGCC	TATCGCGAAATCGAAGAGGC	20	20	50.0	50.0	58.1	58.2	0.0	15.2	0.323012	26301	26465	251-256	1.000000	79.9
rpoC2-rpoC1_p4	rpoC2-rpoC1	4	TGTGAAAGACCAGATTGGCC	TTTCCCAGGCCTGTTCCTAT	20	20	50.0	50.0	58.1	58.3	0.0	30.6	0.409072	26301	26465	220-225	1.000000	79.6
rpoC2-rpoC1_p5	rpoC2-rpoC1	5	TGTGAAAGACCAGATTGGCC	TTTTCCCAGGCCTGTTCCTA	20	20	50.0	50.0	58.1	58.5	0.0	30.6	0.638851	26301	26465	221-226	1.000000	77.8
ORF308_p1	ORF308	1	CCGGTATTGAAACAAACCCG	TCATGGAGAGTCGATCCTGG	20	20	50.0	55.0	57.4	58.3	14.0	5.2	0.925263	33080	33987	1062-1117	1.000000	77.9
ORF308_p2	ORF308	2	CCGGTATTGAAACAAACCCG	AGGCTTAACACATGCACAGA	20	20	50.0	45.0	57.4	57.4	14.0	0.0	1.189379	33080	33987	1021-1076	1.000000	75.9
ORF308_p3	ORF308	3	CCGGTATTGAAACAAACCCG	CAGGCTTAACACATGCACAG	20	20	50.0	50.0	57.4	57.4	14.0	0.0	1.246862	33080	33987	1022-1077	1.000000	75.4
ORF308_p4	ORF308	4	CCGGTATTGAAACAAACCCG	GGCTTAACACATGCACAGAG	20	20	50.0	50.0	57.4	57.1	14.0	0.0	1.530456	33080	33987	1020-1075	1.000000	73.1
ORF308_p5	ORF308	5	TCTTCGTCAAAGTCGGAATCC	TCATGGAGAGTCGATCCTGG	21	20	47.6	55.0	58.0	58.3	0.0	5.2	1.347103	33080	33987	1044-1044	0.500000	49.2
rps14-psaB_p1	rps14-psaB	1	GCCGGAAGCTTGCTTATTTC	ACTTATGCGGCGTTCTTGAT	20	20	50.0	45.0	57.8	58.0	16.4	9.4	0.218975	43504	43626	278-278	1.000000	79.4
rps14-psaB_p2	rps14-psaB	2	GCCGGAAGCTTGCTTATTTC	TTGATTGCCTCGACATCAGG	20	20	50.0	50.0	57.8	58.0	16.4	0.0	0.223220	43504	43626	263-263	1.000000	79.4
rps14-psaB_p3	rps14-psaB	3	GCCGGAAGCTTGCTTATTTC	CCCTTTCCATTGTCCAAGCA	20	20	50.0	50.0	57.8	58.4	16.4	0.0	0.563395	43504	43626	340-340	1.000000	76.4
rps14-psaB_p4	rps14-psaB	4	GCCGGAAGCTTGCTTATTTC	CGTTCTTGATTGCCTCGACA	20	20	50.0	50.0	57.8	58.6	16.4	0.0	0.766351	43504	43626	268-268	1.000000	75.1
rps14-psaB_p5	rps14-psaB	5	GCCGGAAGCTTGCTTATTTC	TACTTATGCGGCGTTCTTGA	20	20	50.0	45.0	57.8	57.3	16.4	9.4	0.853173	43504	43626	279-279	1.000000	74.3
trnT (UGU)-trnL (UAA)_p1	trnT (UGU)-trnL (UAA)	1	GCGATGCTCTACCTTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	52903	53618	728-766	1.000000	84.7
trnT (UGU)-trnL (UAA)_p2	trnT (UGU)-trnL (UAA)	2	GCGATGCTCTACCTTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	52903	53618	736-774	1.000000	84.2
trnT (UGU)-trnL (UAA)_p3	trnT (UGU)-trnL (UAA)	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	52903	53618	760-798	1.000000	83.8
trnT (UGU)-trnL (UAA)_p4	trnT (UGU)-trnL (UAA)	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	52903	53618	768-806	1.000000	83.2
trnT (UGU)-trnL (UAA)_p5	trnT (UGU)-trnL (UAA)	5	GCGATGCTCTACCTTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	52903	53618	742-780	1.000000	82.8
ORF222_p1	ORF222	1	TGATCGAACCGTCCAGTTTC	TCGTACGACCCCACTAAAGA	20	20	50.0	50.0	57.9	57.8	0.0	15.3	0.273374	57339	58007	1420-1430	1.000000	79.3
ORF222_p2	ORF222	2	AAAGCAGAAGCATAGACGCA	TCGTACGACCCCACTAAAGA	20	20	45.0	50.0	57.9	57.8	0.0	15.3	0.297968	57339	58007	1521-1531	1.000000	79.0
ORF222_p3	ORF222	3	TGCTAATGGGGGCTAAGACT	TCGTACGACCCCACTAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	15.3	0.305512	57339	58007	1614-1624	1.000000	78.9
ORF222_p4	ORF222	4	TCGCAGTCTCACCTAGGATT	TCGTACGACCCCACTAAAGA	20	20	50.0	50.0	57.9	57.8	18.0	15.3	0.344224	57339	58007	1267-1277	1.000000	78.9
ORF222_p5	ORF222	5	CTAGAAAGTTTCTCCGGCCC	TCGTACGACCCCACTAAAGA	20	20	55.0	50.0	58.0	57.8	21.2	15.3	0.224415	57339	58007	1636-1636	0.500000	58.2
rpl14-rpl16_p1	rpl14-rpl16	1	TCGACGATTGCTAGCTCCTA	ATGAAATCGGTGGCGTAACA	20	20	50.0	45.0	58.0	57.9	17.2	0.0	0.138898	80302	80470	342-360	1.000000	83.8
rpl14-rpl16_p2	rpl14-rpl16	2	TCGACGATTGCTAGCTCCTA	TCGGTGGCGTAACAGAAAAT	20	20	50.0	45.0	58.0	57.8	17.2	0.0	0.209679	80302	80470	336-354	1.000000	83.3
rpl14-rpl16_p3	rpl14-rpl16	3	TCGACGATTGCTAGCTCCTA	AAATCGGTGGCGTAACAGAA	20	20	50.0	45.0	58.0	57.8	17.2	0.0	0.209679	80302	80470	339-357	1.000000	83.2
rpl14-rpl16_p4	rpl14-rpl16	4	TCGACGATTGCTAGCTCCTA	AATCGGTGGCGTAACAGAAA	20	20	50.0	45.0	58.0	57.8	17.2	0.0	0.209679	80302	80470	338-356	1.000000	83.2
rpl14-rpl16_p5	rpl14-rpl16	5	TCGACGATTGCTAGCTCCTA	ATCGGTGGCGTAACAGAAAA	20	20	50.0	45.0	58.0	57.8	17.2	0.0	0.209679	80302	80470	337-355	1.000000	83.2
ycf1_p1	ycf1	1	CGTGAAAAAGTCCAGAACGC	ACCTCGCGGAGTAATACGTA	20	20	50.0	50.0	58.0	58.0	0.0	6.1	0.065158	109816	109935	488-626	1.000000	89.7
ycf1_p2	ycf1	2	CGTGAAAAAGTCCAGAACGC	GTTCGTCCAGGTCGTATACG	20	20	50.0	55.0	58.0	57.9	0.0	19.2	0.115516	109816	109935	508-646	1.000000	89.2
ycf1_p3	ycf1	3	AGGGCACTTCTTCAATTGGG	ACCTCGCGGAGTAATACGTA	20	20	50.0	50.0	58.1	58.0	5.4	6.1	0.117573	109816	109935	704-782	1.000000	88.0
ycf1_p4	ycf1	4	AAAGGGAAGAAAAGGCACGT	ACCTCGCGGAGTAATACGTA	20	20	45.0	50.0	57.9	58.0	0.0	6.1	0.113807	109816	109935	790-868	1.000000	87.5
ycf1_p5	ycf1	5	CGAGGATCCCGAAGAAAAGG	ACCTCGCGGAGTAATACGTA	20	20	55.0	50.0	58.1	58.0	8.6	6.1	0.105116	109816	109935	960-1038	1.000000	86.9
trnL (UAG)-rpl32_p1	trnL (UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.280977	124854	125325	584-599	1.000000	83.4
trnL (UAG)-rpl32_p2	trnL (UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.282347	124854	125325	585-600	1.000000	83.4
trnL (UAG)-rpl32_p3	trnL (UAG)-rpl32	3	TCCTAAGAGCAGCGTGTCTA	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.397424	124854	125325	594-609	1.000000	82.4
trnL (UAG)-rpl32_p4	trnL (UAG)-rpl32	4	GAAGTAGCAAAGCCTTGGGT	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.362089	124854	125325	1077-1092	1.000000	80.8
trnL (UAG)-rpl32_p5	trnL (UAG)-rpl32	5	TGAAGTAGCAAAGCCTTGGG	GGAAGGGATATTCATCGGCG	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.363447	124854	125325	1078-1093	1.000000	80.8