primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	AGGAGACAGAACACGAAGGA	TTTCGGGGAGAACCAAAAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013495	114323	114442	871-871	0.500000	59.9
ycf1_p2	ycf1	2	AAGGACGTGTACCAGAGGAA	TTTCGGGGAGAACCAAAAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015664	114323	114442	856-856	0.500000	59.9
ycf1_p3	ycf1	3	AAATCGGATTTTCGGCGAGA	TTTCGGGGAGAACCAAAAGG	20	20	45.0	50.0	58.0	58.0	10.5	0.0	0.028836	114323	114442	698-698	0.500000	59.8
ycf1_p4	ycf1	4	AGGAGACAGAACACGAAGGA	AACGTTGTTTTCGGGGAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044453	114323	114442	879-879	0.500000	59.6
ycf1_p5	ycf1	5	AAGGACGTGTACCAGAGGAA	AACGTTGTTTTCGGGGAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.046621	114323	114442	864-864	0.500000	59.6
ycf1-rps15_p1	ycf1-rps15	1	TGGCAGTCGTTTCAGTATGG	ATGAGACGAAGAACACCGTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.159299	117537	117904	902-902	0.500000	58.7
ycf1-rps15_p2	ycf1-rps15	2	GGCAGTCGTTTCAGTATGGT	ATGAGACGAAGAACACCGTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.160755	117537	117904	901-901	0.500000	58.7
ycf1-rps15_p3	ycf1-rps15	3	TGGCAGTCGTTTCAGTATGG	AACCGATCCGCAGTTTTCTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.201097	117537	117904	492-492	0.500000	58.4
ycf1-rps15_p4	ycf1-rps15	4	GGCAGTCGTTTCAGTATGGT	AACCGATCCGCAGTTTTCTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.202553	117537	117904	491-491	0.500000	58.4
ycf1-rps15_p5	ycf1-rps15	5	TGGCAGTCGTTTCAGTATGG	CCAACGGGTTACATAAGGCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.210133	117537	117904	1026-1026	0.500000	58.3
rps15_p1	rps15	1	TGGCAGTCGTTTCAGTATGG	GAAAGGAGTCTGTGCACCAA	20	20	50.0	50.0	57.9	58.0	0.0	15.7	0.125988	117905	118177	1209-1209	0.500000	59.0
rps15_p2	rps15	2	GGCAGTCGTTTCAGTATGGT	GAAAGGAGTCTGTGCACCAA	20	20	50.0	50.0	57.9	58.0	0.0	15.7	0.127444	117905	118177	1208-1208	0.500000	59.0
rps15_p3	rps15	3	TGGCAGTCGTTTCAGTATGG	CATGAGGTAAATCAGCCGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134265	117905	118177	1324-1324	0.500000	58.9
rps15_p4	rps15	4	GGCAGTCGTTTCAGTATGGT	CATGAGGTAAATCAGCCGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.135721	117905	118177	1323-1323	0.500000	58.9
rps15_p5	rps15	5	TGGCAGTCGTTTCAGTATGG	TCTGTGCACCAATATCTGCC	20	20	50.0	50.0	57.9	58.0	0.0	15.7	0.135992	117905	118177	1201-1201	0.500000	58.9
rps15-ndhH_p1	rps15-ndhH	1	TGGCAGTCGTTTCAGTATGG	GAAAGGAGTCTGTGCACCAA	20	20	50.0	50.0	57.9	58.0	0.0	15.7	0.125988	118178	118279	1209-1209	0.500000	59.0
rps15-ndhH_p2	rps15-ndhH	2	GGCAGTCGTTTCAGTATGGT	GAAAGGAGTCTGTGCACCAA	20	20	50.0	50.0	57.9	58.0	0.0	15.7	0.127444	118178	118279	1208-1208	0.500000	59.0
rps15-ndhH_p3	rps15-ndhH	3	TGGCAGTCGTTTCAGTATGG	CTATTCCAGACGCTCGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.132633	118178	118279	1504-1504	0.500000	58.9
rps15-ndhH_p4	rps15-ndhH	4	GGCAGTCGTTTCAGTATGGT	CTATTCCAGACGCTCGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.134089	118178	118279	1503-1503	0.500000	58.9
rps15-ndhH_p5	rps15-ndhH	5	TGGCAGTCGTTTCAGTATGG	CATGAGGTAAATCAGCCGCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134265	118178	118279	1324-1324	0.500000	58.9
ndhH_p1	ndhH	1	AAGAAAACTGCGGATCGGTT	AAGAATTCCCAAAGGGCTGG	20	20	45.0	50.0	58.1	58.1	0.0	0.6	0.165014	118280	119476	1746-1746	0.500000	58.7
ndhH_p2	ndhH	2	AAACTGCGGATCGGTTGAAT	AAGAATTCCCAAAGGGCTGG	20	20	45.0	50.0	58.2	58.1	0.0	0.6	0.236007	118280	119476	1742-1742	0.500000	58.1
ndhH_p3	ndhH	3	AACTGCGGATCGGTTGAATT	AAGAATTCCCAAAGGGCTGG	20	20	45.0	50.0	58.2	58.1	0.0	0.6	0.236007	118280	119476	1741-1741	0.500000	58.1
ndhH_p4	ndhH	4	ACTGCGGATCGGTTGAATTT	AAGAATTCCCAAAGGGCTGG	20	20	45.0	50.0	58.2	58.1	0.0	0.6	0.236007	118280	119476	1740-1740	0.500000	58.1
ndhH_p5	ndhH	5	TGCGGATCGGTTGAATTTCA	AAGAATTCCCAAAGGGCTGG	20	20	45.0	50.0	58.2	58.1	1.9	0.6	0.242431	118280	119476	1738-1738	0.500000	58.1
ndhA_p1	ndhA	1	TCGGTGAAATGACGGAATCC	GACAGACGCGAACACATACT	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.040000	119478	121662	2879-2879	0.500000	59.7
ndhA_p2	ndhA	2	AGCCCCAAAAGGAGAATTGG	GACAGACGCGAACACATACT	20	20	50.0	50.0	58.1	58.0	0.0	15.9	0.078682	119478	121662	2680-2680	0.500000	59.4
ndhA_p3	ndhA	3	AAGAGTCGAAGCCCCAAAAG	GACAGACGCGAACACATACT	20	20	50.0	50.0	58.1	58.0	0.0	15.9	0.123898	119478	121662	2689-2689	0.500000	59.0
ndhA_p4	ndhA	4	TCCGACTTTTGAATTGCCGA	GACAGACGCGAACACATACT	20	20	45.0	50.0	58.1	58.0	0.0	15.9	0.135898	119478	121662	2725-2725	0.500000	58.9
ndhA_p5	ndhA	5	ATGGGGGAAGTTGATCGTTG	GACAGACGCGAACACATACT	20	20	50.0	50.0	57.9	58.0	0.0	15.9	0.150229	119478	121662	2511-2511	0.500000	58.8
ndhI_p1	ndhI	1	GTTGATATAGTTGGGGCGCA	ACCCCATCACCAATAGTCCA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.058397	121744	122247	1686-1686	0.500000	59.5
ndhI_p2	ndhI	2	GTTGATATAGTTGGGGCGCA	TAAGTGGGAATTGATGCGGG	20	20	50.0	50.0	58.0	57.9	10.8	0.0	0.089541	121744	122247	1274-1274	0.500000	59.3
ndhI_p3	ndhI	3	TCTTCCCAACTCCTTTCCCT	ACCCCATCACCAATAGTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.154049	121744	122247	1191-1191	0.500000	58.8
ndhI_p4	ndhI	4	GTTGATATAGTTGGGGCGCA	AATACCACTCCCAGACCTCC	20	20	50.0	55.0	58.0	58.1	10.8	0.0	0.157266	121744	122247	1467-1467	0.500000	58.7
ndhI_p5	ndhI	5	GTTGATATAGTTGGGGCGCA	CCCAAGGAAAAGGCCGAATA	20	20	50.0	50.0	58.0	57.9	10.8	0.0	0.176016	121744	122247	1503-1503	0.500000	58.6
psaC-ndhD_p1	psaC-ndhD	1	CAAATTGCTTCGGCTCCAAG	TTCCCAATCGCCAGTCAAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.025009	124098	124234	543-543	0.500000	59.8
psaC-ndhD_p2	psaC-ndhD	2	AAACAACTCGAAGCATGGGT	TTCCCAATCGCCAGTCAAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.030450	124098	124234	440-440	0.500000	59.8
psaC-ndhD_p3	psaC-ndhD	3	CAAATTGCTTCGGCTCCAAG	AAGACGTTTCTTTCCACCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036246	124098	124234	782-782	0.500000	59.7
psaC-ndhD_p4	psaC-ndhD	4	AAACAACTCGAAGCATGGGT	AAGACGTTTCTTTCCACCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041687	124098	124234	679-679	0.500000	59.7
psaC-ndhD_p5	psaC-ndhD	5	GGCATGAAACAACTCGAAGC	TTCCCAATCGCCAGTCAAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.042603	124098	124234	446-446	0.500000	59.7
ndhD_p1	ndhD	1	CAAATTGCTTCGGCTCCAAG	AAAGGCGTCTGGTCACAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.032046	124235	125761	1969-1969	0.500000	59.7
ndhD_p2	ndhD	2	AAACAACTCGAAGCATGGGT	AAAGGCGTCTGGTCACAATT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.037487	124235	125761	1866-1866	0.500000	59.7
ndhD_p3	ndhD	3	GGCATGAAACAACTCGAAGC	AAAGGCGTCTGGTCACAATT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.049640	124235	125761	1872-1872	0.500000	59.6
ndhD_p4	ndhD	4	ACCGATTACCCACAAAACCC	AAAGGCGTCTGGTCACAATT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.104966	124235	125761	1775-1775	0.500000	59.2
ndhD_p5	ndhD	5	GATTACCCACAAAACCCCGT	AAAGGCGTCTGGTCACAATT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.104966	124235	125761	1772-1772	0.500000	59.2
ccsA_p1	ccsA	1	AATTGTGACCAGACGCCTTT	GTGAAATCGGTAGACACGCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.026574	126072	127031	1293-1293	0.500000	59.8
ccsA_p2	ccsA	2	AATTGTGACCAGACGCCTTT	TGAAATCGGTAGACACGCTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027944	126072	127031	1292-1292	0.500000	59.8
ccsA_p3	ccsA	3	TTATCGATGGCTTCCCTTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	8.5	0.0	0.040512	126072	127031	1803-1803	0.500000	59.7
ccsA_p4	ccsA	4	TTATCGATGGCTTCCCTTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	8.5	0.0	0.041882	126072	127031	1802-1802	0.500000	59.7
ccsA_p5	ccsA	5	TCTGGACCACGGGAGTTATT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.060846	126072	127031	1575-1575	0.500000	59.5
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	CGAAATCCCTTTCTACCGGG	20	20	50.0	55.0	58.0	58.1	0.0	6.3	0.058365	127214	128239	1111-1111	0.500000	59.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	GAGCGGGTACTAATATGCCG	CGAAATCCCTTTCTACCGGG	20	20	55.0	55.0	58.0	58.1	0.0	6.3	0.058365	127214	128239	1655-1655	0.500000	59.5
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CAGCGTGTCTACCGATTTCA	CGAAATCCCTTTCTACCGGG	20	20	50.0	55.0	58.0	58.1	0.0	6.3	0.059735	127214	128239	1112-1112	0.500000	59.5
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	GAAGTAGCAAAGCCTTGGGT	CGAAATCCCTTTCTACCGGG	20	20	50.0	55.0	58.1	58.1	0.0	6.3	0.139477	127214	128239	1608-1608	0.500000	58.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	ATTCATTGGCGGGCTTTTTG	CGAAATCCCTTTCTACCGGG	20	20	45.0	55.0	57.9	58.1	2.4	6.3	0.148214	127214	128239	1203-1203	0.500000	58.8
rpl32_p1	rpl32	1	ATGCAAGTGCGAGATATGGG	AGGTACCACCAATAACTCGGA	20	21	50.0	47.6	58.1	58.2	0.0	15.9	1.287826	128240	128398	899-899	0.500000	49.7
rpl32_p2	rpl32	2	ATTGGAATGCAAGTGCGAGA	AGGTACCACCAATAACTCGGA	20	21	45.0	47.6	58.2	58.2	0.0	15.9	1.350951	128240	128398	905-905	0.500000	49.2
rpl32_p3	rpl32	3	TTGGAATGCAAGTGCGAGAT	AGGTACCACCAATAACTCGGA	20	21	45.0	47.6	58.2	58.2	0.0	15.9	1.350951	128240	128398	904-904	0.500000	49.2
rpl32_p4	rpl32	4	GCAAGTGCGAGATATGGGAT	AGGTACCACCAATAACTCGGA	20	21	50.0	47.6	57.8	58.2	0.0	15.9	1.351919	128240	128398	897-897	0.500000	49.2
rpl32_p5	rpl32	5	TGCCTAAATGGGAAAGGGTG	AGGTACCACCAATAACTCGGA	20	21	50.0	47.6	57.8	58.2	0.0	15.9	1.406170	128240	128398	934-934	0.500000	48.8
rpl32-ndhF_p1	rpl32-ndhF	1	ATGCAAGTGCGAGATATGGG	AACAGAAGCCCCACTCCTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.139198	128399	129385	1698-1698	0.500000	58.9
rpl32-ndhF_p2	rpl32-ndhF	2	ATGCAAGTGCGAGATATGGG	ACAGAAGCCCCACTCCTATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.139198	128399	129385	1697-1697	0.500000	58.9
rpl32-ndhF_p3	rpl32-ndhF	3	ATTGGAATGCAAGTGCGAGA	ACAGAAGCCCCACTCCTATT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.202322	128399	129385	1703-1703	0.500000	58.4
rpl32-ndhF_p4	rpl32-ndhF	4	TTGGAATGCAAGTGCGAGAT	AACAGAAGCCCCACTCCTAT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.202322	128399	129385	1703-1703	0.500000	58.4
rpl32-ndhF_p5	rpl32-ndhF	5	TTGGAATGCAAGTGCGAGAT	ACAGAAGCCCCACTCCTATT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.202322	128399	129385	1702-1702	0.500000	58.4
ndhF_p1	ndhF	1	GGGGAAAGGTATGAGTCATCC	GCAGAATACCGTCACCCATT	21	20	52.4	50.0	57.5	58.0	9.4	0.0	1.532154	129386	131614	2951-2951	0.500000	47.7
ndhF_p2	ndhF	2	GGGGAAAGGTATGAGTCATCC	AGGGGGACTAAACAGGAACA	21	20	52.4	50.0	57.5	57.9	9.4	0.0	1.621418	129386	131614	2856-2856	0.500000	47.0
ndhF_p3	ndhF	3	GGGGAAAGGTATGAGTCATCC	CGAGGAAACGGAAGAGATCC	21	20	52.4	55.0	57.5	57.8	9.4	0.0	1.705120	129386	131614	2767-2767	0.500000	46.4
ndhF_p4	ndhF	4	GGGGAAAGGTATGAGTCATCC	ACAACTTTCGAAAGGAGGGG	21	20	52.4	50.0	57.5	57.7	9.4	34.5	1.765711	129386	131614	2871-2871	0.500000	45.9
ndhF_p5	ndhF	5	GGGGAAAGGTATGAGTCATCC	AAGGAGGGGGACTAAACAGG	21	20	52.4	55.0	57.5	58.3	9.4	0.0	1.823708	129386	131614	2860-2860	0.500000	45.4