primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	CGGAGATACCCCTTCCCTTA	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.398212	8798	9594	883-907	1.000000	80.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	CGGAGATACCCCTTCCCTTA	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.399600	8798	9594	882-906	1.000000	80.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGTATCAAGCGGGTCGAGTA	CGGAGATACCCCTTCCCTTA	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.442074	8798	9594	967-991	1.000000	80.0
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GAGCGGAGAAAGAGGGATTC	CGGAGATACCCCTTCCCTTA	20	20	55.0	55.0	57.8	57.6	0.0	0.0	0.626386	8798	9594	939-963	1.000000	78.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GTCGAGTAATCAGAGCGAGC	CGGAGATACCCCTTCCCTTA	20	20	55.0	55.0	58.3	57.6	0.0	0.0	0.644477	8798	9594	955-979	1.000000	78.4
atpF-atpH_p1	atpF-atpH	1	AGTGGCCCAAGTAAACGAAA	TGGTTGTGGCATTAGCACTT	20	20	45.0	45.0	57.6	58.0	0.0	0.0	0.357750	13647	14158	596-596	1.000000	77.8
atpF-atpH_p2	atpF-atpH	2	CAGTGGCCCAAGTAAACGAA	TGGTTGTGGCATTAGCACTT	20	20	50.0	45.0	58.4	58.0	0.0	0.0	0.406021	13647	14158	597-597	1.000000	77.4
atpF-atpH_p3	atpF-atpH	3	TGGCCCAAGTAAACGAAAGA	TGGTTGTGGCATTAGCACTT	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.648198	13647	14158	594-594	1.000000	75.5
atpF-atpH_p4	atpF-atpH	4	GTGGCCCAAGTAAACGAAAG	TGGTTGTGGCATTAGCACTT	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.705295	13647	14158	595-595	1.000000	75.0
atpF-atpH_p5	atpF-atpH	5	TTAAACCCGAAACTTCCGGC	TGGTTGTGGCATTAGCACTT	20	20	50.0	45.0	58.8	58.0	0.0	0.0	0.768511	13647	14158	624-624	1.000000	74.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.117235	14405	15887	1572-1609	1.000000	80.7
atpH-atpI_p2	atpH-atpI	2	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	20	20	50.0	45.0	58.0	57.9	3.3	0.0	0.126227	14405	15887	1655-1692	1.000000	80.6
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.191128	14405	15887	1628-1665	1.000000	80.1
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.262774	14405	15887	1573-1610	1.000000	79.6
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	20	20	55.0	45.0	58.2	57.9	0.0	0.0	0.269165	14405	15887	1632-1669	1.000000	79.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AATTCTTCCGTCAAGCCCTC	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	57.9	58.1	0.0	14.6	0.186475	28242	29441	1344-1350	1.000000	79.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AATTCTTCCGTCAAGCCCTC	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.591585	28242	29441	1370-1376	1.000000	76.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AATTCTTCCGTCAAGCCCTC	CCTTACCACTTGGCCATGTC	20	20	50.0	55.0	57.9	58.5	0.0	20.8	0.651669	28242	29441	1324-1330	1.000000	75.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AAATTCTTCCGTCAAGCCCT	AAAGGATTTGCAGTCCCCTG	20	20	45.0	50.0	57.4	58.1	0.0	14.6	0.657780	28242	29441	1345-1351	1.000000	75.6
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTTCCGTCAAGCCCTCATTA	AAAGGATTTGCAGTCCCCTG	20	20	50.0	50.0	57.3	58.1	0.0	14.6	0.762541	28242	29441	1340-1346	1.000000	74.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	ATTTGGATCGGATTTGGCGA	AGTCCACTCCTTCCCCATAC	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.170394	29513	30392	1008-1052	1.000000	82.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	ATTTGGATCGGATTTGGCGA	GTCCACTCCTTCCCCATACT	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.170394	29513	30392	1007-1051	1.000000	82.4
trnC-GCA-petN_p3	trnC-GCA-petN	3	ATTTGGATCGGATTTGGCGA	AAAGTCCACTCCTTCCCCAT	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.290929	29513	30392	1010-1054	1.000000	81.4
trnC-GCA-petN_p4	trnC-GCA-petN	4	ATTTGGATCGGATTTGGCGA	CCTCCCTAAAGTCCACTCCT	20	20	45.0	55.0	57.9	57.7	0.0	0.0	0.307105	29513	30392	1017-1061	1.000000	81.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTTGGATCGGATTTGGCGA	TACCCCTCCCTAAAGTCCAC	20	20	45.0	55.0	57.9	57.7	0.0	0.0	0.310348	29513	30392	1021-1065	1.000000	81.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.149342	33769	34629	979-1000	1.000000	80.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.207439	33769	34629	958-979	1.000000	80.2
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.229593	33769	34629	955-976	1.000000	80.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33769	34629	943-964	1.000000	79.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33769	34629	922-943	1.000000	79.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49620	50790	1197-1221	1.000000	81.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	49620	50790	1228-1252	1.000000	81.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49620	50790	1205-1229	1.000000	80.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	49620	50790	1236-1260	1.000000	80.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	49620	50790	1220-1244	1.000000	79.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	68907	70191	1417-1432	1.000000	77.0
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	68907	70191	1415-1430	1.000000	76.9
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.565192	68907	70191	1430-1445	1.000000	76.6
psbE-petL_p4	psbE-petL	4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.569541	68907	70191	1428-1443	1.000000	76.6
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.647263	68907	70191	1426-1441	1.000000	76.0
psbT-psbN_p1	psbT-psbN	1	TTTTCGAGAACCGCCTAAGG	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.113125	78888	78951	133-133	1.000000	83.9
psbT-psbN_p2	psbT-psbN	2	ACCGCCTAAGGTTCCAACTA	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.4	58.0	0.0	3.3	0.376060	78888	78951	124-124	1.000000	82.2
psbT-psbN_p3	psbT-psbN	3	TTTTCGAGAACCGCCTAAGG	TTCGAAGAACACGGGGACTA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.469295	78888	78951	128-128	1.000000	81.2
psbT-psbN_p4	psbT-psbN	4	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.9	57.7	0.0	0.0	0.373212	78888	78951	182-182	1.000000	80.5
psbT-psbN_p5	psbT-psbN	5	TTTTCGAGAACCGCCTAAGG	GCAACCCTCTCGACAACTAA	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.539559	78888	78951	156-156	1.000000	79.8
psbH_p1	psbH	1	TTGCTCTGGTATCTTTCGCC	GCCAAGCTAAACTCAAGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113118	79204	79425	1319-1345	1.000000	80.1
psbH_p2	psbH	2	TCAACTAGTCCCCGTGTTCT	GCCAAGCTAAACTCAAGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094313	79204	79425	983-1003	1.000000	79.9
psbH_p3	psbH	3	CCCGTGTTCTTCGAATGGAT	GCCAAGCTAAACTCAAGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113118	79204	79425	973-993	1.000000	79.7
psbH_p4	psbH	4	TCAACTAGTCCCCGTGTTCT	TTTTGGAGTCCGGTTTCGAG	20	20	50.0	50.0	58.0	58.1	0.0	4.3	0.131672	79204	79425	805-805	0.500000	58.9
psbH_p5	psbH	5	CCCGTGTTCTTCGAATGGAT	TTTTGGAGTCCGGTTTCGAG	20	20	50.0	50.0	58.0	58.1	0.0	4.3	0.150478	79204	79425	795-795	0.500000	58.8
petB_p1	petB	1	CCATTCTGGTAGTTCGACCG	TCTATCGTTCCTCGCCAGTA	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.414375	79619	81077	1629-1662	1.000000	78.3
petB_p2	petB	2	CCATTCTGGTAGTTCGACCG	ATTGTCTATCGTTCCTCGCC	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.588197	79619	81077	1633-1666	1.000000	76.9
petB_p3	petB	3	CCATTCTGGTAGTTCGACCG	ATCGTTCCTCGCCAGTATGA	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.677716	79619	81077	1626-1659	1.000000	76.2
petB_p4	petB	4	CCATTCTGGTAGTTCGACCG	TCGTTCCTCGCCAGTATGAT	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.677716	79619	81077	1625-1658	1.000000	76.2
petB_p5	petB	5	GGTAGTTCGACCGTGGAATT	TCTATCGTTCCTCGCCAGTA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.712118	79619	81077	1622-1655	1.000000	76.0
rpl16_p1	rpl16	1	ACACATCAATTCTCGGGCTC	GGTTTCGAAGGAATTGCACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066091	86034	87538	2320-2332	1.000000	82.1
rpl16_p2	rpl16	2	ACACATCAATTCTCGGGCTC	GTTTCGAAGGAATTGCACGG	20	20	50.0	50.0	58.0	58.0	0.0	14.0	0.066091	86034	87538	2319-2331	1.000000	82.1
rpl16_p3	rpl16	3	ACACATCAATTCTCGGGCTC	GGACGGATCAACGGAAAAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.118788	86034	87538	1990-2002	1.000000	82.1
rpl16_p4	rpl16	4	GTATATGCGCGAGACACCAT	GGTTTCGAAGGAATTGCACG	20	20	50.0	50.0	57.9	58.0	12.1	0.0	0.102246	86034	87538	2741-2753	1.000000	81.4
rpl16_p5	rpl16	5	GTATATGCGCGAGACACCAT	GTTTCGAAGGAATTGCACGG	20	20	50.0	50.0	57.9	58.0	12.1	14.0	0.102246	86034	87538	2740-2752	1.000000	81.4
ndhB_p1	ndhB	1	GTTCATAACCCCAGCCCATT	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	100095	102327	2363-2363	1.000000	74.8
ndhB_p2	ndhB	2	GTTCATAACCCCAGCCCATT	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	100095	102327	2364-2364	1.000000	74.8
ndhB_p3	ndhB	3	TTTGGGCGGAACAGATCTAC	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.6	57.2	0.0	0.0	1.184063	100095	102327	2338-2338	1.000000	72.9
ndhB_p4	ndhB	4	TTTGGGCGGAACAGATCTAC	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	57.6	57.2	0.0	0.0	1.184063	100095	102327	2339-2339	1.000000	72.9
ndhB_p5	ndhB	5	TTGGGCGGAACAGATCTACT	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	58.4	57.2	0.0	0.0	1.218969	100095	102327	2337-2337	1.000000	72.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	AGGAGTAGGATCCATTGTGTCT	20	22	50.0	45.5	57.9	58.0	0.0	15.8	2.125206	122719	122746	113-113	1.000000	63.7
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TAAGCCGCCATGGTGAAATT	AGGAGTAGGATCCATTGTGTCT	20	22	45.0	45.5	58.2	58.0	27.1	15.8	2.154922	122719	122746	135-135	1.000000	63.4
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AAATTGGTAGACACGCTGCT	AGGAGTAGGATCCATTGTGTCT	20	22	45.0	45.5	57.8	58.0	0.0	15.8	2.185933	122719	122746	120-120	1.000000	63.2
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GGTAGACACGCTGCTCTTAG	AGGAGTAGGATCCATTGTGTCT	20	22	55.0	45.5	57.8	58.0	0.0	15.8	2.197223	122719	122746	115-115	1.000000	63.1
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTAGACACGCTGCTCTTAGG	AGGAGTAGGATCCATTGTGTCT	20	22	55.0	45.5	57.8	58.0	0.0	15.8	2.197223	122719	122746	114-114	1.000000	63.1
psaC-ndhE_p1	psaC-ndhE	1	CGTCCCACGGTATCATTTCC	AACGCGTATCAACCAATCGA	20	20	55.0	45.0	58.4	58.0	0.0	14.5	0.422369	125957	126809	896-987	1.000000	81.6
psaC-ndhE_p2	psaC-ndhE	2	CGTCCCACGGTATCATTTCC	ACGCGTATCAACCAATCGAA	20	20	55.0	45.0	58.4	58.0	0.0	11.7	0.422369	125957	126809	895-986	1.000000	81.6
psaC-ndhE_p3	psaC-ndhE	3	CGTCCCACGGTATCATTTCC	CAACGCGTATCAACCAATCG	20	20	55.0	50.0	58.4	57.9	0.0	14.5	0.493022	125957	126809	897-988	1.000000	81.1
psaC-ndhE_p4	psaC-ndhE	4	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	20	20	50.0	45.0	58.6	58.0	0.0	14.5	0.562300	125957	126809	863-954	1.000000	80.6
psaC-ndhE_p5	psaC-ndhE	5	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	20	20	50.0	45.0	58.6	58.0	0.0	11.7	0.562300	125957	126809	862-953	1.000000	80.6
rps15-ycf1_p1	rps15-ycf1	1	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGA	23	20	34.8	45.0	56.3	56.3	4.0	0.0	6.401264	132637	133078	614-639	1.000000	44.3
rps15-ycf1_p2	rps15-ycf1	2	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGAA	23	21	34.8	42.9	56.3	57.1	4.0	0.0	6.633284	132637	133078	614-639	1.000000	44.3
rps15-ycf1_p3	rps15-ycf1	3	ACTTGAAATTCAACAGAACCTCT	GGCCCAATTACCGATTAGAAGA	23	22	34.8	45.5	56.3	57.9	4.0	0.0	6.778787	132637	133078	613-638	1.000000	44.3
rps15-ycf1_p4	rps15-ycf1	4	ACTTGAAATTCAACAGAACCTC	TGGCCCAATTACCGATTAGA	22	20	36.4	45.0	54.9	56.3	4.0	0.0	6.844850	132637	133078	614-639	1.000000	44.3
rps15-ycf1_p5	rps15-ycf1	5	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGAAG	23	22	34.8	45.5	56.3	58.2	4.0	0.0	6.881656	132637	133078	614-639	1.000000	44.3