primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbT-psbN_p1 psbT-psbN 1 TTTCGAGAACCACCTAAGGT ACCCTAGTCGCCATCTTTCT 20 20 45.0 50.0 56.1 58.1 0.0 0.0 2.037345 75118 75191 236-236 1.000000 66.4 psbT-psbN_p2 psbT-psbN 2 TTTCGAGAACCACCTAAGGT GATCCATTCGAGGAACACGG 20 20 45.0 55.0 56.1 58.4 0.0 0.0 2.332789 75118 75191 143-143 1.000000 65.8 psbT-psbN_p3 psbT-psbN 3 TTTTCGAGAACCACCTAAGGT ACCCTAGTCGCCATCTTTCT 21 20 42.9 50.0 56.9 58.1 0.0 0.0 2.263154 75118 75191 237-237 1.000000 64.6 psbT-psbN_p4 psbT-psbN 4 TTTCGAGAACCACCTAAGGT GGCAACCCTCTCAACAACTA 20 20 45.0 50.0 56.1 57.4 0.0 0.0 2.466186 75118 75191 166-166 1.000000 64.1 psbT-psbN_p5 psbT-psbN 5 AGGTTCCAACTAAAAGGGTCA ACCCTAGTCGCCATCTTTCT 21 20 42.9 50.0 56.8 58.1 0.0 0.0 2.381224 75118 75191 220-220 1.000000 63.9 ycf1_p1 ycf1 1 AGTCCTTTCCTCATTTGCGG TTTCGGGGAGAACCAAAAGG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.183909 112524 112643 1068-1068 0.500000 58.5 ycf1_p2 ycf1 2 GTCCTTTCCTCATTTGCGGA TTTCGGGGAGAACCAAAAGG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.189077 112524 112643 1067-1067 0.500000 58.5 ycf1_p3 ycf1 3 AGTCCTTTCCTCATTTGCGG GTGCCAAGGTTTCAGACAGA 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.206297 112524 112643 949-949 0.500000 58.3 ycf1_p4 ycf1 4 AGTCCTTTCCTCATTTGCGG TCTGTGCCAAGGTTTCAGAC 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.206297 112524 112643 952-952 0.500000 58.3 ycf1_p5 ycf1 5 GTCCTTTCCTCATTTGCGGA GTGCCAAGGTTTCAGACAGA 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.211465 112524 112643 948-948 0.500000 58.3 ndhH_p1 ndhH 1 TCAGAGGGGTTTGCGAAAAA CCCGAGACTGAATAAACGCA 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.064633 116875 118056 1749-1749 0.500000 59.5 ndhH_p2 ndhH 2 TCAGAGGGGTTTGCGAAAAA AGTGTCCTAACGAATCGCAC 20 20 45.0 50.0 57.9 58.0 0.0 1.2 0.067880 116875 118056 2019-2019 0.500000 59.5 ndhH_p3 ndhH 3 TCAGAGGGGTTTGCGAAAAA TTAGTTCATCGCCCCGAATG 20 20 45.0 50.0 57.9 58.1 0.0 0.0 0.111946 116875 118056 2121-2121 0.500000 59.1 ndhH_p4 ndhH 4 TCAGAGGGGTTTGCGAAAAA TGAAGAATTCCCAAAGGGCC 20 20 45.0 50.0 57.9 58.1 0.0 0.6 0.122859 116875 118056 1667-1667 0.500000 59.0 ndhH_p5 ndhH 5 TATCTCAGAGGGGTTTGCGA CCCGAGACTGAATAAACGCA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.143942 116875 118056 1753-1753 0.500000 58.8 ndhA_p1 ndhA 1 TGTGAGAGTAGAAGCCCCAA TGACCAATATACCTTGCGGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.062382 118058 120222 2517-2517 0.500000 59.5 ndhA_p2 ndhA 2 TGTGAGAGTAGAAGCCCCAA CTCGGACTGTTTGTTGACCA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.076071 118058 120222 2498-2498 0.500000 59.4 ndhA_p3 ndhA 3 TGTGAGAGTAGAAGCCCCAA GGCATACACGAACACAGACT 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.104462 118058 120222 2669-2669 0.500000 59.2 ndhA_p4 ndhA 4 AAATTGGGGCTTATCCGGAC TGACCAATATACCTTGCGGC 20 20 50.0 50.0 57.9 58.0 6.1 0.0 0.105572 118058 120222 2853-2853 0.500000 59.2 ndhA_p5 ndhA 5 AAATTGGGGCTTATCCGGAC CTCGGACTGTTTGTTGACCA 20 20 50.0 50.0 57.9 58.1 6.1 0.0 0.119261 118058 120222 2834-2834 0.500000 59.0 ndhI_p1 ndhI 1 TTTTGGGGGTGGAATCTGTG GCTGCGATTCTCACAACCTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.018388 120301 120852 1206-1206 0.500000 59.9 ndhI_p2 ndhI 2 GGTTTTTGGGGGTGGAATCT GCTGCGATTCTCACAACCTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.034321 120301 120852 1209-1209 0.500000 59.7 ndhI_p3 ndhI 3 GGAATCTCGCTATTCCGGAC GCTGCGATTCTCACAACCTA 20 20 55.0 50.0 57.9 58.0 1.8 0.0 0.077559 120301 120852 977-977 0.500000 59.4 ndhI_p4 ndhI 4 ACAGTTCTTTACTTGGGCGG GCTGCGATTCTCACAACCTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.117936 120301 120852 999-999 0.500000 59.1 ndhI_p5 ndhI 5 CAGTTCTTTACTTGGGCGGT GCTGCGATTCTCACAACCTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.117936 120301 120852 998-998 0.500000 59.1 ndhI-ndhG_p1 ndhI-ndhG 1 TGGTCAACAAACAGTCCGAG AATCCCAAGTTGTTGACCCC 20 20 50.0 50.0 58.1 58.0 0.0 1.4 0.061577 120853 121400 1506-1506 0.500000 59.5 ndhI-ndhG_p2 ndhI-ndhG 2 AGTCTGTGTTCGTGTATGCC AATCCCAAGTTGTTGACCCC 20 20 50.0 50.0 57.9 58.0 0.0 1.4 0.089967 120853 121400 1335-1335 0.500000 59.3 ndhI-ndhG_p3 ndhI-ndhG 3 TGCCCTATTGATCTACCCGT AATCCCAAGTTGTTGACCCC 20 20 50.0 50.0 57.9 58.0 0.0 1.4 0.106791 120853 121400 1319-1319 0.500000 59.1 ndhI-ndhG_p4 ndhI-ndhG 4 TGGTCAACAAACAGTCCGAG AATCCCGTACCAGGACGTAT 20 20 50.0 50.0 58.1 57.9 0.0 1.1 0.129583 120853 121400 1437-1437 0.500000 59.0 ndhI-ndhG_p5 ndhI-ndhG 5 TGGTCAACAAACAGTCCGAG ATAATCCCGTACCAGGACGT 20 20 50.0 50.0 58.1 57.9 0.0 1.1 0.129583 120853 121400 1439-1439 0.500000 59.0 ndhE_p1 ndhE 1 GGGGTCAACAACTTGGGATT TGCTACGAGTTGTCTCATGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.013390 122172 122477 1163-1163 0.500000 59.9 ndhE_p2 ndhE 2 GGGGTCAACAACTTGGGATT TCCTCTGTTCTTGGAGCAGA 20 20 50.0 50.0 58.0 58.0 0.0 12.1 0.025138 122172 122477 1074-1074 0.500000 59.8 ndhE_p3 ndhE 3 GGGGTCAACAACTTGGGATT CCCATGCTACGAGTTGTCTC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.081786 122172 122477 1167-1167 0.500000 59.3 ndhE_p4 ndhE 4 TTTTGGACCGTTGGAGATGG TGCTACGAGTTGTCTCATGC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.099422 122172 122477 1308-1308 0.500000 59.2 ndhE_p5 ndhE 5 GGGGTCAACAACTTGGGATT GTCCTCTGTTCTTGGAGCAG 20 20 50.0 55.0 58.0 57.9 0.0 5.3 0.101534 122172 122477 1075-1075 0.500000 59.2 psaC_p1 psaC 1 TCGCATGAGCAAATCCAGAA ACAAACGTTTCTTCCCTCCC 20 20 45.0 50.0 57.9 58.0 0.0 11.4 0.135324 122753 122998 1028-1028 0.500000 58.9 psaC_p2 psaC 2 CCGAAGCGGCTATTGGATTA ACAAACGTTTCTTCCCTCCC 20 20 50.0 50.0 57.8 58.0 1.4 11.4 0.186762 122753 122998 1249-1249 0.500000 58.5 psaC_p3 psaC 3 TCGCATGAGCAAATCCAGAA TTTGACCGCTGTACATTGCT 20 20 45.0 45.0 57.9 58.1 0.0 0.0 0.221504 122753 122998 920-920 0.500000 58.2 psaC_p4 psaC 4 TCGCATGAGCAAATCCAGAA CCCCCGCAGTGTACAAAATA 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.229207 122753 122998 1064-1064 0.500000 58.2 psaC_p5 psaC 5 TCGCATGAGCAAATCCAGAA TCAAATGGATGCAGGTGGAG 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.245661 122753 122998 428-428 0.500000 58.0 ndhD_p1 ndhD 1 GCATGAGACAACTCGTAGCA GCAAGGCGTAAATTCTGCAA 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.349808 123151 124683 2400-2400 0.500000 57.2 ndhD_p2 ndhD 2 GCATGAGACAACTCGTAGCA TTGCAAGGCGTAAATTCTGC 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.349808 123151 124683 2402-2402 0.500000 57.2 ndhD_p3 ndhD 3 TCTGCTCCAAGAACAGAGGA GCAAGGCGTAAATTCTGCAA 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.361556 123151 124683 2489-2489 0.500000 57.1 ndhD_p4 ndhD 4 TCTGCTCCAAGAACAGAGGA TTGCAAGGCGTAAATTCTGC 20 20 50.0 45.0 58.0 57.7 0.0 0.0 0.361556 123151 124683 2491-2491 0.500000 57.1 ndhD_p5 ndhD 5 GAGACAACTCGTAGCATGGG GCAAGGCGTAAATTCTGCAA 20 20 55.0 45.0 58.1 57.7 0.0 0.0 0.418204 123151 124683 2396-2396 0.500000 56.7 ndhD-ccsA_p1 ndhD-ccsA 1 TCTGGACCACGAGAACTCTT GCGAGATCCAAGACGTGAAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.075307 124684 125234 1126-1126 0.500000 59.4 ndhD-ccsA_p2 ndhD-ccsA 2 TTCTGGACCACGAGAACTCT GCGAGATCCAAGACGTGAAT 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.075307 124684 125234 1127-1127 0.500000 59.4 ndhD-ccsA_p3 ndhD-ccsA 3 TCTGGACCACGAGAACTCTT ACCCAAGGCTTTGCTACTTC 20 20 50.0 50.0 58.0 58.1 0.0 2.4 0.092144 124684 125234 1364-1364 0.500000 59.3 ndhD-ccsA_p4 ndhD-ccsA 4 TCTGGACCACGAGAACTCTT GACCCAAGGCTTTGCTACTT 20 20 50.0 50.0 58.0 58.1 0.0 2.4 0.092144 124684 125234 1365-1365 0.500000 59.3 ndhD-ccsA_p5 ndhD-ccsA 5 TTCTGGACCACGAGAACTCT ACCCAAGGCTTTGCTACTTC 20 20 50.0 50.0 58.0 58.1 0.0 2.4 0.092144 124684 125234 1365-1365 0.500000 59.3 ccsA_p1 ccsA 1 TCTGGACCACGAGAACTCTT TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.0 58.1 0.0 2.2 0.070180 125235 126206 2124-2124 0.500000 59.4 ccsA_p2 ccsA 2 TTCTGGACCACGAGAACTCT TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.0 58.1 0.0 2.2 0.070180 125235 126206 2125-2125 0.500000 59.4 ccsA_p3 ccsA 3 TCTGGACCACGAGAACTCTT GAACAGAACTCTCCGGTTCC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.084927 125235 126206 2107-2107 0.500000 59.3 ccsA_p4 ccsA 4 TTCTGGACCACGAGAACTCT GAACAGAACTCTCCGGTTCC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.084927 125235 126206 2108-2108 0.500000 59.3 ccsA_p5 ccsA 5 TCTGGACCACGAGAACTCTT TAGACACGCTGCTCTTAGGA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.127479 125235 126206 1848-1848 0.500000 59.0 ccsA-trnL-UAG_p1 ccsA-trnL-UAG 1 ATTCACGTCTTGGATCTCGC TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.1 58.1 0.0 2.2 0.139084 126207 126305 1018-1018 0.500000 58.9 ccsA-trnL-UAG_p2 ccsA-trnL-UAG 2 ATTCACGTCTTGGATCTCGC GAACAGAACTCTCCGGTTCC 20 20 50.0 55.0 58.1 57.9 0.0 0.0 0.153830 126207 126305 1001-1001 0.500000 58.8 ccsA-trnL-UAG_p3 ccsA-trnL-UAG 3 AAGTAGCAAAGCCTTGGGTC TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.1 58.1 0.0 2.2 0.155921 126207 126305 779-779 0.500000 58.8 ccsA-trnL-UAG_p4 ccsA-trnL-UAG 4 GAAGTAGCAAAGCCTTGGGT TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.1 58.1 0.0 2.2 0.155921 126207 126305 780-780 0.500000 58.8 ccsA-trnL-UAG_p5 ccsA-trnL-UAG 5 TGAAGTAGCAAAGCCTTGGG TTCTCTGCCGGGACTAAGAA 20 20 50.0 50.0 58.1 58.1 0.0 2.2 0.157279 126207 126305 781-781 0.500000 58.7 trnL-UAG-rpl32_p1 trnL-UAG-rpl32 1 AAGTAGCAAAGCCTTGGGTC CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.107485 126386 127396 2101-2101 0.500000 59.1 trnL-UAG-rpl32_p2 trnL-UAG-rpl32 2 GAAGTAGCAAAGCCTTGGGT CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.107485 126386 127396 2102-2102 0.500000 59.1 trnL-UAG-rpl32_p3 trnL-UAG-rpl32 3 TGAAGTAGCAAAGCCTTGGG CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.108842 126386 127396 2103-2103 0.500000 59.1 trnL-UAG-rpl32_p4 trnL-UAG-rpl32 4 TCCTAAGAGCAGCGTGTCTA CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.142819 126386 127396 1618-1618 0.500000 58.9 trnL-UAG-rpl32_p5 trnL-UAG-rpl32 5 AAGTAGCAAAGCCTTGGGTC TCATTTTTGCATGGCGGTTC 20 20 50.0 45.0 58.1 57.9 0.0 0.0 0.166355 126386 127396 1708-1708 0.500000 58.7 rpl32_p1 rpl32 1 CCCATCCCGCAAAAACCTAT CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.243157 127397 127561 824-824 0.500000 58.1 rpl32_p2 rpl32 2 ACCCCACTCCCATTTTTGAG CGCGGGTAAGGATTCTGAAA 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.327678 127397 127561 1303-1303 0.500000 57.4 rpl32_p3 rpl32 3 CCCATCCCGCAAAAACCTAT GACGAAAATCGCCGGAGATA 20 20 50.0 50.0 58.2 57.9 0.0 21.7 0.354438 127397 127561 719-719 0.500000 57.2 rpl32_p4 rpl32 4 CCCATCCCGCAAAAACCTAT ACGAAAATCGCCGGAGATAC 20 20 50.0 50.0 58.2 58.2 0.0 21.7 0.375518 127397 127561 718-718 0.500000 57.0 rpl32_p5 rpl32 5 CCCATCCCGCAAAAACCTAT CGAAAATCGCCGGAGATACA 20 20 50.0 50.0 58.2 58.2 0.0 21.7 0.376797 127397 127561 717-717 0.500000 57.0 rpl32-ndhF_p1 rpl32-ndhF 1 AGTACGTTTCTTCGGAACCG ACAATTGGTCGTGTGAACCA 20 20 50.0 45.0 57.9 58.0 6.9 10.8 0.101656 127562 128703 1678-1678 0.500000 59.2 rpl32-ndhF_p2 rpl32-ndhF 2 AGTACGTTTCTTCGGAACCG CGAATAAGAGGGAGGCACAC 20 20 50.0 55.0 57.9 58.1 6.9 0.0 0.114769 127562 128703 1869-1869 0.500000 59.1 rpl32-ndhF_p3 rpl32-ndhF 3 AGTACGTTTCTTCGGAACCG AGGTACGAGCACATTCCAAC 20 20 50.0 50.0 57.9 57.9 6.9 0.0 0.150856 127562 128703 1645-1645 0.500000 58.8 rpl32-ndhF_p4 rpl32-ndhF 4 AGTACGTTTCTTCGGAACCG GGTACGAGCACATTCCAACT 20 20 50.0 50.0 57.9 57.9 6.9 0.0 0.150856 127562 128703 1644-1644 0.500000 58.8 rpl32-ndhF_p5 rpl32-ndhF 5 AGTACGTTTCTTCGGAACCG AAGCCCCACTCCTATTGCTA 20 20 50.0 50.0 57.9 58.1 6.9 0.0 0.162779 127562 128703 1244-1244 0.500000 58.7 ndhF_p1 ndhF 1 CTCGTAGCGTTTCCCGTAAA CCGGGTGAATGGAAAGGAAA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.100874 128704 130932 2977-2977 0.500000 59.2 ndhF_p2 ndhF 2 ATTATTGGCGATTGGCGGAT ACGAAGGGGACTAAACAGGA 20 20 45.0 50.0 58.1 58.0 1.5 0.0 0.110108 128704 130932 2942-2942 0.500000 59.1 ndhF_p3 ndhF 3 CTCGTAGCGTTTCCCGTAAA AAGAGATCCGGGTGAATGGA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.139171 128704 130932 2984-2984 0.500000 58.9 ndhF_p4 ndhF 4 CTCGTAGCGTTTCCCGTAAA AGAGATCCGGGTGAATGGAA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.139171 128704 130932 2983-2983 0.500000 58.9 ndhF_p5 ndhF 5 ATTATTGGCGATTGGCGGAT CCTTCCCCTTCCCTTTGTTC 20 20 45.0 55.0 58.1 58.1 1.5 0.0 0.165569 128704 130932 2897-2897 0.500000 58.7