primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	CAAAACCCGTAGCCTTACCA	TTTGGCGAAGAAAAAGCGAC	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.301972	6907	7246	444-459	1.000000	79.9
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	AAAACCCGTAGCCTTACCAC	TTTGGCGAAGAAAAAGCGAC	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.303504	6907	7246	443-458	1.000000	79.9
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	ACCAAAACCCGTAGCCTTAC	TTTGGCGAAGAAAAAGCGAC	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.303504	6907	7246	446-461	1.000000	79.9
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	GAAGGATTCGAACCTCCGAG	TTTGGCGAAGAAAAAGCGAC	20	20	55.0	45.0	57.8	57.9	16.4	0.0	0.325263	6907	7246	473-488	1.000000	79.6
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	CAAAACCCGTAGCCTTACCA	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	57.8	57.8	0.0	15.8	0.350381	6907	7246	450-465	1.000000	79.5
trnG-UCC_p1	trnG-UCC	1	TAGCAGTGGAATGAATGGGC	TGAGCAAGCAGAAACCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.128915	9025	9846	1531-1541	1.000000	81.5
trnG-UCC_p2	trnG-UCC	2	GCAAAGGAAAGGGGTAGCTT	TGAGCAAGCAGAAACCCTTT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.147046	9025	9846	1513-1523	1.000000	81.4
trnG-UCC_p3	trnG-UCC	3	TAGCAGTGGAATGAATGGGC	TGCTCAAACAACCCCAATCA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.136307	9025	9846	1721-1731	1.000000	81.1
trnG-UCC_p4	trnG-UCC	4	TAGCAGTGGAATGAATGGGC	GCCGGCAAATCAAAATTGGA	20	20	50.0	45.0	57.9	57.9	0.0	24.8	0.148990	9025	9846	1842-1852	1.000000	80.9
trnG-UCC_p5	trnG-UCC	5	TAGCAGTGGAATGAATGGGC	ACAAGTAGCCGGCAAATCAA	20	20	50.0	45.0	57.9	58.1	0.0	31.5	0.152851	9025	9846	1849-1859	1.000000	80.8
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9847	9984	209-218	1.000000	75.0
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9847	9984	212-221	1.000000	74.6
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9847	9984	215-224	1.000000	74.0
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9847	9984	218-227	1.000000	73.6
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9847	9984	213-222	1.000000	72.3
atpF-atpH_p1	atpF-atpH	1	TTAAACCCGAAACTCCCAGC	CCCGAAGCAGAGGGTAAAAT	20	20	50.0	50.0	58.1	57.6	0.0	2.6	0.519813	13021	13451	619-622	1.000000	76.6
atpF-atpH_p2	atpF-atpH	2	TTAAACCCGAAACTCCCAGC	ATTTATGGGTTGGTCGTGGC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.629704	13021	13451	553-556	1.000000	75.8
atpF-atpH_p3	atpF-atpH	3	GGCCAGTGACCAAAGGAAAT	CCCGAAGCAGAGGGTAAAAT	20	20	50.0	50.0	58.4	57.6	0.0	2.6	0.787470	13021	13451	595-598	1.000000	74.5
atpF-atpH_p4	atpF-atpH	4	TTAAACCCGAAACTCCCAGC	ACGATTTATGGGTTGGTCGT	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.873747	13021	13451	556-559	1.000000	73.9
atpF-atpH_p5	atpF-atpH	5	GGCCAGTGACCAAAGGAAAT	ATTTATGGGTTGGTCGTGGC	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.897361	13021	13451	529-532	1.000000	73.7
rps2-rpoC2_p1	rps2-rpoC2	1	GCTTCCATCATCTCTGCCAA	TGGTTCTTGCATCCCAAACA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.127614	15819	16049	319-324	1.000000	82.2
rps2-rpoC2_p2	rps2-rpoC2	2	TTGCAGAGATGTAAGGTGCC	TGGTTCTTGCATCCCAAACA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.193030	15819	16049	384-389	1.000000	81.1
rps2-rpoC2_p3	rps2-rpoC2	3	GCAGAGATGTAAGGTGCCAT	TGGTTCTTGCATCCCAAACA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.417746	15819	16049	382-387	1.000000	79.4
rps2-rpoC2_p4	rps2-rpoC2	4	CCTGCTTCCATCATCTCTGC	TGGTTCTTGCATCCCAAACA	20	20	55.0	45.0	58.4	57.9	0.0	0.0	0.483703	15819	16049	322-327	1.000000	79.3
rps2-rpoC2_p5	rps2-rpoC2	5	CTGCTTCCATCATCTCTGCC	TGGTTCTTGCATCCCAAACA	20	20	55.0	45.0	58.4	57.9	0.0	0.0	0.483703	15819	16049	321-326	1.000000	79.3
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TGGTTCAAATCCAATAGTAGGT	GAGTGGAGGCTGTCAATGTT	22	20	36.4	50.0	54.8	57.8	0.0	0.0	5.414263	49129	49312	238-239	1.000000	47.7
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TGGTTCAAATCCAATAGTAGGT	TAGACGAGCTAGGACACGAG	22	20	36.4	55.0	54.8	58.1	0.0	0.0	5.285857	49129	49312	255-256	1.000000	47.2
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TGGTTCAAATCCAATAGTAGGT	GCTGAAGGCAAGAGACAAGT	22	20	36.4	50.0	54.8	58.1	0.0	0.0	5.333590	49129	49312	296-297	1.000000	46.2
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TGGTTCAAATCCAATAGTAGGT	GAGCTGAAGGCAAGAGACAA	22	20	36.4	50.0	54.8	57.8	0.0	0.0	5.399013	49129	49312	298-299	1.000000	46.2
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TGGTTCAAATCCAATAGTAGGT	AGCTGAAGGCAAGAGACAAG	22	20	36.4	50.0	54.8	57.8	0.0	0.0	5.405089	49129	49312	297-298	1.000000	46.2
atpE_p1	atpE	1	GGTACCTAAATCGGCACCTG	CTGCTCCTGCTACGACATTT	20	20	55.0	50.0	58.1	58.0	4.1	0.0	0.066270	49313	49717	1513-1515	1.000000	81.2
atpE_p2	atpE	2	TAAATCGGCACCTGAATCGG	CTGCTCCTGCTACGACATTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067632	49313	49717	1507-1509	1.000000	81.2
atpE_p3	atpE	3	GGTACCTAAATCGGCACCTG	GCAGTTTATGTACCTGCGGA	20	20	55.0	50.0	58.1	58.0	4.1	5.5	0.069077	49313	49717	1547-1549	1.000000	81.1
atpE_p4	atpE	4	GGTACCTAAATCGGCACCTG	TTGGTTTGACTGCCCTAACC	20	20	55.0	50.0	58.1	58.0	4.1	0.0	0.060828	49313	49717	1777-1779	1.000000	81.0
atpE_p5	atpE	5	TAAATCGGCACCTGAATCGG	TTGGTTTGACTGCCCTAACC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.062189	49313	49717	1771-1773	1.000000	80.9
rbcL-accD_p1	rbcL-accD	1	ATCAAATTCGAATTCGCCGC	ATTGAGCCAACGTTTTCCCA	20	20	45.0	45.0	57.9	58.3	22.9	0.0	0.406817	53289	53969	688-738	1.000000	82.7
rbcL-accD_p2	rbcL-accD	2	ATCAAATTCGAATTCGCCGC	TTGAGCCAACGTTTTCCCAT	20	20	45.0	45.0	57.9	58.3	22.9	0.0	0.406817	53289	53969	687-737	1.000000	82.7
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	ATTGAGCCAACGTTTTCCCA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.415354	53289	53969	712-762	1.000000	82.5
rbcL-accD_p4	rbcL-accD	4	GCTGCTTGTGAGGTATGGAA	TTGAGCCAACGTTTTCCCAT	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.415354	53289	53969	711-761	1.000000	82.5
rbcL-accD_p5	rbcL-accD	5	ATCAAATTCGAATTCGCCGC	ATTTACTTAGCCCACACCCG	20	20	45.0	50.0	57.9	57.6	22.9	0.0	0.518632	53289	53969	737-787	1.000000	81.6
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	ATTATAGGCACTGCAACGGG	20	20	45.0	50.0	58.2	58.0	0.0	2.8	0.203702	59754	60741	1190-1190	1.000000	80.7
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	TATAGGCACTGCAACGGGTA	20	20	45.0	50.0	58.2	58.5	0.0	2.8	0.683721	59754	60741	1188-1188	1.000000	76.8
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	TGCAACGGGTATTCTTGTGA	20	20	45.0	45.0	58.2	57.4	0.0	0.0	0.723620	59754	60741	1179-1179	1.000000	76.5
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	ACTGCAACGGGTATTCTTGT	20	20	45.0	45.0	58.2	57.4	0.0	0.0	0.732499	59754	60741	1181-1181	1.000000	76.4
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	ATTATAGGCACTGCAACGGG	20	20	45.0	50.0	57.1	58.0	0.0	2.8	0.964193	59754	60741	1189-1189	1.000000	74.6
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	61516	62789	1421-1425	1.000000	77.2
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	61516	62789	1419-1423	1.000000	77.2
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.647263	61516	62789	1430-1434	1.000000	76.2
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.651612	61516	62789	1428-1432	1.000000	76.2
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.660168	61516	62789	1435-1439	1.000000	76.1
psbT-psbN_p1	psbT-psbN	1	TTTTCGAGAACCGCCTAAGG	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104406	70986	71043	214-214	1.000000	80.3
psbT-psbN_p2	psbT-psbN	2	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.9	57.7	0.0	0.0	0.373212	70986	71043	176-176	1.000000	78.4
psbT-psbN_p3	psbT-psbN	3	TTTTCGAGAACCGCCTAAGG	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.9	58.4	0.0	0.0	0.517031	70986	71043	128-128	1.000000	77.7
psbT-psbN_p4	psbT-psbN	4	TTTTCGAGAACCGCCTAAGG	GTCGCCATCTCCATATCTGG	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.465757	70986	71043	215-215	1.000000	77.4
psbT-psbN_p5	psbT-psbN	5	TTTTCGAGAACCGCCTAAGG	GGCAACCCTCTCAACAACTA	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.650428	70986	71043	151-151	1.000000	76.4
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	CACCCAATCAAATTTGGCCC	20	20	50.0	50.0	58.0	57.9	7.6	17.9	0.121581	83121	83200	208-208	1.000000	81.0
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	CCACCCAATCAAATTTGGCC	20	20	50.0	50.0	58.0	57.9	7.6	17.9	0.121581	83121	83200	209-209	1.000000	80.9
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TCATTGGTGTGCATCCAACA	CACCCAATCAAATTTGGCCC	20	20	45.0	50.0	58.0	57.9	22.4	17.9	0.119842	83121	83200	259-259	1.000000	80.6
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	TCATTGGTGTGCATCCAACA	CCACCCAATCAAATTTGGCC	20	20	45.0	50.0	58.0	57.9	22.4	17.9	0.119842	83121	83200	260-260	1.000000	80.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TCATTGGTGTGCATCCAACA	CCCACCCAATCAAATTTGGC	20	20	45.0	50.0	58.0	57.9	22.4	17.9	0.119842	83121	83200	261-261	1.000000	80.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CCATTTCTACCGGGCATTCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	6.3	0.0	0.059165	90358	91349	1079-1086	1.000000	84.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAGGGATATCAAGCCGCTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	4.8	0.0	0.034958	90358	91349	1123-1130	1.000000	84.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAGGGATATCAAGCCGCTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	4.8	0.0	0.036329	90358	91349	1124-1131	1.000000	84.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CCATTTCTACCGGGCATTCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	6.3	0.0	0.060535	90358	91349	1080-1087	1.000000	84.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAGGGATATCAAGCCGCTG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	4.8	0.0	0.151405	90358	91349	1133-1140	1.000000	83.6
ccsA-psaC_p1	ccsA-psaC	1	GGGTGTGGATTCGGCAATTA	GAATCTGCTTGTCCAACCGA	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.433907	92480	94198	1913-1915	1.000000	77.5
ccsA-psaC_p2	ccsA-psaC	2	GGGTGTGGATTCGGCAATTA	CCAAGAACCGAAGACTGTGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.482811	92480	94198	1949-1951	1.000000	77.1
ccsA-psaC_p3	ccsA-psaC	3	GGGTGTGGATTCGGCAATTA	TCTGCTTGTCCAACCGATTT	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.512670	92480	94198	1910-1912	1.000000	76.9
ccsA-psaC_p4	ccsA-psaC	4	GGGTGTGGATTCGGCAATTA	TGAATCTGCTTGTCCAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.719649	92480	94198	1914-1916	1.000000	75.2
ccsA-psaC_p5	ccsA-psaC	5	GGGTGTGGATTCGGCAATTA	ATCTGCTTGTCCAACCGATT	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.739216	92480	94198	1911-1913	1.000000	75.1
psaC-rps15_p1	psaC-rps15	1	ATACATCTGTGGGACAGGCT	TGAGTTAGGTATTCGAGAACCA	20	22	50.0	40.9	58.1	56.3	4.0	17.6	3.800437	94445	95708	1369-1374	1.000000	51.4
psaC-rps15_p2	psaC-rps15	2	ACATCCGTCCCAAGGTATCA	TGAGTTAGGTATTCGAGAACCA	20	22	50.0	40.9	58.1	56.3	0.0	17.6	3.811356	94445	95708	1395-1400	1.000000	51.3
psaC-rps15_p3	psaC-rps15	3	GACAGGCTCGTACACATTGA	TGAGTTAGGTATTCGAGAACCA	20	22	50.0	40.9	57.6	56.3	0.0	17.6	4.049273	94445	95708	1357-1362	1.000000	49.4
psaC-rps15_p4	psaC-rps15	4	ACAGGCTCGTACACATTGAG	TGAGTTAGGTATTCGAGAACCA	20	22	50.0	40.9	57.6	56.3	0.0	17.6	4.055767	94445	95708	1356-1361	1.000000	49.4
psaC-rps15_p5	psaC-rps15	5	CAGGCTCGTACACATTGAGT	TGAGTTAGGTATTCGAGAACCA	20	22	50.0	40.9	57.6	56.3	0.0	17.6	4.055767	94445	95708	1355-1360	1.000000	49.4
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	15.9	18.9	0.366304	102148	102666	624-628	1.000000	82.6
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	102148	102666	611-615	1.000000	82.6
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	57.8	57.8	4.4	18.9	0.421214	102148	102666	612-616	1.000000	82.2
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.526686	102148	102666	654-658	1.000000	81.2
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	57.7	57.8	0.0	18.9	0.543107	102148	102666	603-607	1.000000	81.2
trnA-UGC-trnI-GAU_p1	trnA-UGC-trnI-GAU	1	TACCGCCTGCACAATTAGAC	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023125	107372	107435	197-197	1.000000	80.6
trnA-UGC-trnI-GAU_p2	trnA-UGC-trnI-GAU	2	TACCGCCTGCACAATTAGAC	ATTGGTTGGATCGTAGGTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056929	107372	107435	219-219	1.000000	80.3
trnA-UGC-trnI-GAU_p3	trnA-UGC-trnI-GAU	3	TACCGCCTGCACAATTAGAC	AGGTCTCTGGTTCAAGTCCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.121695	107372	107435	179-179	1.000000	79.9
trnA-UGC-trnI-GAU_p4	trnA-UGC-trnI-GAU	4	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	4.9	0.0	0.341137	107372	107435	161-161	1.000000	78.3
trnA-UGC-trnI-GAU_p5	trnA-UGC-trnI-GAU	5	TACCGCCTGCACAATTAGAC	CGAGGTCTCTGGTTCAAGTC	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.374259	107372	107435	181-181	1.000000	77.9