primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	65421	66585	1300-1339	1.000000	74.5
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	65421	66585	1349-1388	1.000000	71.3
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	65421	66585	1348-1387	1.000000	71.3
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	20	21	45.0	47.6	58.2	57.5	0.0	4.1	1.673144	65421	66585	1307-1346	1.000000	69.6
petA-psbJ_p5	petA-psbJ	5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	20	21	45.0	47.6	58.2	58.5	0.0	0.0	1.703141	65421	66585	1349-1388	1.000000	69.3
rps19-rpl2_p1	rps19-rpl2	1	GCCCGGGACCAAGTTACTAT	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	59.2	58.0	29.3	22.4	1.188900	86013	86079	250-256	1.000000	74.6
rps19-rpl2_p2	rps19-rpl2	2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	59.2	58.2	29.3	35.0	1.317396	86013	86079	264-270	1.000000	73.3
rps19-rpl2_p3	rps19-rpl2	3	GCCCGGGACCAAGTTACTAT	GGCCCCGATTGGTAGAAAAA	20	20	55.0	50.0	59.2	58.2	29.3	0.0	1.322672	86013	86079	288-294	1.000000	73.0
rps19-rpl2_p4	rps19-rpl2	4	CCCGGGACCAAGTTACTATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	56.6	58.0	18.5	22.4	1.412176	86013	86079	249-255	1.000000	72.8
rps19-rpl2_p5	rps19-rpl2	5	GCCCGGGACCAAGTTACTAT	TATCCTGCGCTTGGAAGAAG	20	20	55.0	50.0	59.2	57.7	29.3	22.4	1.480649	86013	86079	248-254	1.000000	72.3
ndhF_p1	ndhF	1	TTTCACGCATGTCCAGTTGA	GTACGTTTCTTTGGAACCGC	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.344835	111620	113851	2963-2978	0.416667	54.5
ndhF_p2	ndhF	2	ACGAAGGGGACTAAACAGGA	AATAAGGGCTGGTGAATCGG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.368218	111620	113851	2934-2946	0.416667	54.2
ndhF_p3	ndhF	3	TTTCACGCATGTCCAGTTGA	AATAAGGGCTGGTGAATCGG	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.387994	111620	113851	2658-2670	0.416667	54.1
ndhF_p4	ndhF	4	ACAGGAACAAGAGGGATCCA	AATAAGGGCTGGTGAATCGG	20	20	50.0	50.0	58.0	57.7	15.2	0.0	0.393203	111620	113851	2920-2932	0.416667	54.0
ndhF_p5	ndhF	5	AAGAGGGATCCACCGAAGAA	AATAAGGGCTGGTGAATCGG	20	20	50.0	50.0	58.0	57.7	15.2	0.0	0.395789	111620	113851	2912-2924	0.416667	54.0
ndhF-rpl32_p1	ndhF-rpl32	1	CGGAACAAACAGAAGTCCCA	ACCGGATATCCCCTTTCCTT	20	20	50.0	50.0	58.0	57.8	0.0	16.4	0.246133	113852	114197	511-520	0.500000	59.3
ndhF-rpl32_p2	ndhF-rpl32	2	ACAAACAGAAGTCCCAACCC	ACCGGATATCCCCTTTCCTT	20	20	50.0	50.0	57.9	57.8	0.0	16.4	0.275292	113852	114197	507-516	0.500000	59.0
ndhF-rpl32_p3	ndhF-rpl32	3	AATAGGGAGGCACACAGAGT	ACCGGATATCCCCTTTCCTT	20	20	50.0	50.0	58.0	57.8	0.0	16.4	0.249638	113852	114197	1119-1128	0.500000	58.7
ndhF-rpl32_p4	ndhF-rpl32	4	AAGGGGAAATTGTGCGGATT	ACCGGATATCCCCTTTCCTT	20	20	45.0	50.0	58.1	57.8	0.0	16.4	0.270735	113852	114197	1159-1168	0.500000	58.5
ndhF-rpl32_p5	ndhF-rpl32	5	AGGGGAAATTGTGCGGATTT	ACCGGATATCCCCTTTCCTT	20	20	45.0	50.0	58.1	57.8	0.0	16.4	0.270735	113852	114197	1158-1167	0.500000	58.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGGAACAAACAGAAGTCCCA	ACAGAAAAAGGCAGCCATCA	20	20	50.0	45.0	58.0	58.0	0.0	15.8	0.038558	114372	115547	2117-2141	0.500000	60.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGGAACAAACAGAAGTCCCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046037	114372	115547	1814-1838	0.500000	60.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGAACAAACAGAAGTCCCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047407	114372	115547	1815-1839	0.500000	60.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACAAACAGAAGTCCCAACCC	ACAGAAAAAGGCAGCCATCA	20	20	50.0	45.0	57.9	58.0	0.0	15.8	0.067717	114372	115547	2113-2137	0.500000	60.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACAAACAGAAGTCCCAACCC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075196	114372	115547	1810-1834	0.500000	60.5
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	ACAGAAAAAGGCAGCCATCA	20	20	50.0	45.0	58.0	58.0	0.0	15.8	0.008182	115628	115726	323-323	0.500000	60.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	ACAGAAAAAGGCAGCCATCA	20	20	50.0	45.0	58.0	58.0	0.0	15.8	0.009553	115628	115726	322-322	0.500000	60.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	CAATAAGACCCCCATGCCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020948	115628	115726	910-910	0.500000	60.2
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	CAATAAGACCCCCATGCCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.022318	115628	115726	909-909	0.500000	60.2
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTGAAATCGGTAGACACGCT	ACTGGGATTGTAGAGCAGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049542	115628	115726	573-573	0.500000	60.2
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.019370	115727	116677	1486-1486	0.500000	60.7
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020741	115727	116677	1485-1485	0.500000	60.7
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	115727	116677	1710-1710	0.500000	60.6
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024937	115727	116677	1709-1709	0.500000	60.6
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	CTCGATGGCTTCTCTTGCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057002	115727	116677	1708-1708	0.500000	60.3
ndhD_p1	ndhD	1	GGGTGTGAATTCGGCAATTG	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020540	116904	118439	2102-2102	0.500000	60.6
ndhD_p2	ndhD	2	GAGGCATGGGGGTCTTATTG	TCTGCTCCAAGAACAGAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.028574	116904	118439	2209-2209	0.500000	60.5
ndhD_p3	ndhD	3	GGGTGTGAATTCGGCAATTG	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.034922	116904	118439	2066-2066	0.500000	60.5
ndhD_p4	ndhD	4	GGGTGTGAATTCGGCAATTG	CTATTGGGCCGGCTATTGTT	20	20	50.0	50.0	58.0	58.0	0.0	28.6	0.023828	116904	118439	2577-2577	0.500000	60.4
ndhD_p5	ndhD	5	GAGGCATGGGGGTCTTATTG	CTATTGGGCCGGCTATTGTT	20	20	55.0	50.0	58.0	58.0	0.0	28.6	0.031861	116904	118439	2684-2684	0.500000	60.3
ndhG-ndhI_p1	ndhG-ndhI	1	TAATCCCGTACCACGACGTA	TTCCCTATGGTGACTGGGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.085005	120146	120501	1263-1267	0.500000	59.6
ndhG-ndhI_p2	ndhG-ndhI	2	CCCCAACGGTCCAAAGATAG	TTCCCTATGGTGACTGGGTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.092134	120146	120501	1182-1186	0.500000	59.5
ndhG-ndhI_p3	ndhG-ndhI	3	ACAAACCAGTGAAGTGACCC	TTCCCTATGGTGACTGGGTT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.106529	120146	120501	1205-1209	0.500000	59.4
ndhG-ndhI_p4	ndhG-ndhI	4	TAATCCCGTACCACGACGTA	TTGTGGTAATTGCGTCGAGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.189093	120146	120501	949-953	0.500000	58.8
ndhG-ndhI_p5	ndhG-ndhI	5	CCCCAACGGTCCAAAGATAG	TTGTGGTAATTGCGTCGAGT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.196221	120146	120501	868-872	0.500000	58.8
ndhI_p1	ndhI	1	TAATCCCGTACCACGACGTA	TTTTGGGGGTGGAATCTGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039501	120502	121044	1830-1834	0.500000	59.9
ndhI_p2	ndhI	2	TAATCCCGTACCACGACGTA	GTTTTGGGGGTGGAATCTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040940	120502	121044	1831-1835	0.500000	59.9
ndhI_p3	ndhI	3	CCCCAACGGTCCAAAGATAG	TTTTGGGGGTGGAATCTGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.046629	120502	121044	1749-1753	0.500000	59.9
ndhI_p4	ndhI	4	CCCCAACGGTCCAAAGATAG	GTTTTGGGGGTGGAATCTGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048068	120502	121044	1750-1754	0.500000	59.8
ndhI_p5	ndhI	5	TAATCCCGTACCACGACGTA	TTTCATGTGCGGTTCTGGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056993	120502	121044	1940-1944	0.416667	56.4
ndhA_p1	ndhA	1	AACCCAGTCACCATAGGGAA	AAAGCCTTCTCCCACCTTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.054823	121123	123308	2532-2550	0.500000	59.8
ndhA_p2	ndhA	2	AACCCAGTCACCATAGGGAA	CAATGTTACGAGCTTCCGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.058329	121123	123308	2806-2824	0.500000	59.8
ndhA_p3	ndhA	3	AACCCAGTCACCATAGGGAA	TGTGAGAGTAGAAGCCCCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.077923	121123	123308	2490-2508	0.500000	59.7
ndhA_p4	ndhA	4	AACCCAGTCACCATAGGGAA	AGTTGAAGGAGTGGGCATTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.138606	121123	123308	2865-2883	0.500000	59.2
ndhA_p5	ndhA	5	ACTCGACGCAATTACCACAA	AAAGCCTTCTCCCACCTTTG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.158910	121123	123308	2846-2864	0.500000	59.0
ndhH_p1	ndhH	1	CCATCTGCTAGGGCTTGAAG	TTCTTTTGGGGGTGCAGAAA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.202354	123310	124491	2008-2010	0.500000	58.7
ndhH_p2	ndhH	2	CCATCTGCTAGGGCTTGAAG	TTTCTTTTGGGGGTGCAGAA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.202354	123310	124491	2009-2011	0.500000	58.7
ndhH_p3	ndhH	3	CCATCTGCTAGGGCTTGAAG	TTTTCTTTTGGGGGTGCAGA	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.202354	123310	124491	2010-2012	0.500000	58.7
ndhH_p4	ndhH	4	CCATCTGCTAGGGCTTGAAG	AGGTCTGCGGAAAATTCTGG	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.209063	123310	124491	1647-1647	0.500000	58.5
ndhH_p5	ndhH	5	CCATCTGCTAGGGCTTGAAG	GTCTGCGGAAAATTCTGGGA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.214231	123310	124491	1645-1645	0.500000	58.4
rps15_p1	rps15	1	AACACCATGCATTGATGGGT	CTCCTAGGCGTCGTAGAGAA	20	20	45.0	55.0	58.1	58.0	8.9	3.0	0.107795	124599	124865	1123-1125	0.500000	59.7
rps15_p2	rps15	2	TACGACTCAGCTCCAGCATA	CTCCTAGGCGTCGTAGAGAA	20	20	50.0	55.0	57.9	58.0	0.5	3.0	0.101954	124599	124865	1379-1381	0.500000	59.6
rps15_p3	rps15	3	TATACGACTCAGCTCCAGCA	CTCCTAGGCGTCGTAGAGAA	20	20	50.0	55.0	57.9	58.0	0.5	3.0	0.101954	124599	124865	1381-1383	0.500000	59.6
rps15_p4	rps15	4	CAATGCCCACTCCTTCAACT	CTCCTAGGCGTCGTAGAGAA	20	20	50.0	55.0	58.1	58.0	0.0	3.0	0.134031	124599	124865	1673-1675	0.500000	59.3
rps15_p5	rps15	5	TCCGCCATAAAAGGTCCAAG	CTCCTAGGCGTCGTAGAGAA	20	20	50.0	55.0	57.9	58.0	0.0	3.0	0.171955	124599	124865	1419-1421	0.500000	59.1
rps15-ycf1_p1	rps15-ycf1	1	AACGAGTTTTTGGTTCCCGA	CTCCTAGGCGTCGTAGAGAA	20	20	45.0	55.0	58.0	58.0	0.0	3.0	0.090423	124866	125247	939-941	0.500000	60.0
rps15-ycf1_p2	rps15-ycf1	2	ACGAGTTTTTGGTTCCCGAA	CTCCTAGGCGTCGTAGAGAA	20	20	45.0	55.0	58.0	58.0	0.0	3.0	0.090423	124866	125247	938-940	0.500000	60.0
rps15-ycf1_p3	rps15-ycf1	3	AACGAGTTTTTGGTTCCCGA	GAGAAATGCGCCTGGATGTA	20	20	45.0	50.0	58.0	58.0	0.0	4.2	0.087692	124866	125247	1242-1244	0.500000	59.9
rps15-ycf1_p4	rps15-ycf1	4	ACGAGTTTTTGGTTCCCGAA	GAGAAATGCGCCTGGATGTA	20	20	45.0	50.0	58.0	58.0	0.0	4.2	0.087692	124866	125247	1241-1243	0.500000	59.9
rps15-ycf1_p5	rps15-ycf1	5	ACGAGTTTTTGGTTCCCGAA	ATGCGCCTGGATGTAATGTT	20	20	45.0	45.0	58.0	57.9	0.0	4.2	0.098388	124866	125247	1236-1238	0.500000	59.9
ycf1_p1	ycf1	1	TGCCGAATATGTTTCTGCGA	CCTTTTGGTTCTCCCCGAAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027432	127586	127705	527-527	0.500000	60.8
ycf1_p2	ycf1	2	TGCCGAATATGTTTCTGCGA	GTTCTCCCCGAAAACAACCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.040082	127586	127705	520-520	0.500000	60.8
ycf1_p3	ycf1	3	TACGACGTCGATTGACCCTA	CCTTTTGGTTCTCCCCGAAA	20	20	50.0	50.0	58.0	58.0	41.5	0.0	0.033058	127586	127705	1126-1126	0.500000	60.6
ycf1_p4	ycf1	4	TGCCGAATATGTTTCTGCGA	TTTCTGTCTGAAACCCTGGC	20	20	45.0	50.0	58.0	58.0	0.0	1.1	0.035881	127586	127705	648-648	0.500000	60.6
ycf1_p5	ycf1	5	TACGACGTCGATTGACCCTA	TTTCTGTCTGAAACCCTGGC	20	20	50.0	50.0	58.0	58.0	41.5	1.1	0.041508	127586	127705	1247-1247	0.500000	60.4