primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	118	492	1065-1091	1.000000	79.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	118	492	1071-1097	1.000000	79.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	118	492	1066-1092	1.000000	79.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	118	492	1067-1093	1.000000	79.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	118	492	1079-1105	1.000000	79.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4375	5180	941-942	1.000000	76.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4375	5180	940-941	1.000000	76.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4375	5180	1026-1029	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.554139	4375	5180	1025-1028	1.000000	76.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4375	5180	956-957	1.000000	75.9
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8608	9426	970-980	1.000000	79.9
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8608	9426	952-962	1.000000	77.8
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8608	9426	906-916	1.000000	76.2
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8608	9426	971-981	1.000000	75.8
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8608	9426	888-898	1.000000	74.0
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	0.0	0.0	2.573941	10399	10900	661-668	1.000000	61.2
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	0.0	0.0	2.785752	10399	10900	661-668	1.000000	59.5
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.1	55.6	0.0	0.0	3.315377	10399	10900	662-669	1.000000	55.3
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.9	55.6	0.0	0.0	3.324543	10399	10900	653-660	1.000000	55.2
petN-psbM_p5	petN-psbM	5	GCTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	59.1	55.6	0.0	0.0	3.524972	10399	10900	650-657	1.000000	53.6
atpF-atpA_p1	atpF-atpA	1	TTACATTGAGCGTGAGAGCC	CACCCGCCATTACTTCATCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047103	28392	28456	729-732	1.000000	80.6
atpF-atpA_p2	atpF-atpA	2	TTACATTGAGCGTGAGAGCC	CCGTTTCGGCTACCACAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023082	28392	28456	1260-1263	1.000000	80.4
atpF-atpA_p3	atpF-atpA	3	ATCAGTTTCGCGTGAATGGA	CCGTTTCGGCTACCACAATA	20	20	45.0	50.0	57.9	58.0	12.9	0.0	0.106243	28392	28456	1025-1028	1.000000	79.9
atpF-atpA_p4	atpF-atpA	4	TCAGTTTCGCGTGAATGGAT	CCGTTTCGGCTACCACAATA	20	20	45.0	50.0	57.9	58.0	12.9	0.0	0.106243	28392	28456	1024-1027	1.000000	79.9
atpF-atpA_p5	atpF-atpA	5	TCATGTTTGGTTCGGGAAGG	CCGTTTCGGCTACCACAATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108650	28392	28456	1224-1227	1.000000	79.8
atpA-trnR-UCU_p1	atpA-trnR-UCU	1	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.9	58.3	0.0	20.2	1.423066	29984	30171	356-389	1.000000	73.7
atpA-trnR-UCU_p2	atpA-trnR-UCU	2	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.9	58.3	0.0	20.2	1.424529	29984	30171	360-393	1.000000	73.7
atpA-trnR-UCU_p3	atpA-trnR-UCU	3	ATTCAGGAACAGGTGGAACG	GCAATGAAAAGCGTCCATTGT	20	21	50.0	42.9	57.8	58.3	0.0	20.2	1.508929	29984	30171	318-351	1.000000	73.6
atpA-trnR-UCU_p4	atpA-trnR-UCU	4	CATTCACCGAGGAAGCAGAA	GCAATGAAAAGCGTCCATTG	20	20	50.0	45.0	57.9	56.6	0.0	17.2	1.525779	29984	30171	356-389	1.000000	72.9
atpA-trnR-UCU_p5	atpA-trnR-UCU	5	AAGACATTCACCGAGGAAGC	GCAATGAAAAGCGTCCATTG	20	20	50.0	45.0	57.9	56.6	0.0	17.2	1.527243	29984	30171	360-393	1.000000	72.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAATGCCACGG	TTTACCAAGGGCTATAGTCA	20	20	55.0	40.0	58.1	53.0	0.0	6.7	5.054330	31474	32679	1283-1302	1.000000	42.1
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	CGTAAGCCCTCGGTTCAAAT	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.256100	31474	32679	1258-1277	1.000000	42.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGACCCGTTCAGGAATTCA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	57.8	53.0	7.9	6.7	5.169175	31474	32679	1326-1345	1.000000	42.0
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	CAGTGACCCGTTCAGGAATT	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	57.8	53.0	0.0	6.7	5.175267	31474	32679	1328-1347	1.000000	42.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TACAGTGACCCGTTCAGGAA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.283157	31474	32679	1330-1349	1.000000	42.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46687	47223	582-610	1.000000	83.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46687	47223	614-642	1.000000	81.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46687	47223	576-604	1.000000	81.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	46687	47223	608-636	1.000000	80.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	46687	47223	581-609	1.000000	80.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	48179	48805	650-764	1.000000	74.4
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	48179	48805	649-763	1.000000	74.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	48179	48805	657-771	1.000000	73.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	48179	48805	651-765	1.000000	72.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	48179	48805	650-764	1.000000	71.4
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	60541	61324	964-1022	1.000000	80.0
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	60541	61324	896-954	1.000000	79.6
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	60541	61324	928-986	1.000000	79.5
ycf4-cemA_p4	ycf4-cemA	4	GAGCCATTCCATTGACTCGT	AAATCCACCAGGGCAAAACA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.229905	60541	61324	961-1019	1.000000	79.0
ycf4-cemA_p5	ycf4-cemA	5	GAGCCATTCCATTGACTCGT	AATCCACCAGGGCAAAACAA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.229905	60541	61324	960-1018	1.000000	79.0
petA-psbJ_p1	petA-psbJ	1	CTGACGGGAGCAAGAGTAAC	AGCCCATTCGGAAGGATTTC	20	20	55.0	50.0	58.0	57.9	0.0	3.6	0.060924	63218	64007	1371-1383	1.000000	81.5
petA-psbJ_p2	petA-psbJ	2	CTGACGGGAGCAAGAGTAAC	TGGAGGGAAGTGGGGTAAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.071162	63218	64007	1296-1308	1.000000	81.5
petA-psbJ_p3	petA-psbJ	3	CTGACGGGAGCAAGAGTAAC	TTAGGAGGCCTCAATGACCA	20	20	55.0	50.0	58.0	58.0	0.0	33.3	0.036917	63218	64007	1698-1710	1.000000	81.3
petA-psbJ_p4	petA-psbJ	4	CTGACGGGAGCAAGAGTAAC	TCCCTATTCATTGCGGGTTG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.053665	63218	64007	1862-1874	1.000000	81.0
petA-psbJ_p5	petA-psbJ	5	GAGCTATCGCCCCAATCAAA	TTAGGAGGCCTCAATGACCA	20	20	50.0	50.0	58.0	58.0	0.0	33.3	0.067267	63218	64007	1871-1883	1.000000	81.0
clpP_p1	clpP	1	TCACGAATTGACCCGGAATC	GCCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.0	56.9	0.7	0.0	1.157912	70054	72072	2111-2122	1.000000	71.7
clpP_p2	clpP	2	CTCGGCAGGGGTAATTTCAA	GCCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.262153	70054	72072	2132-2143	1.000000	70.8
clpP_p3	clpP	3	AACTCGGCAGGGGTAATTTC	GCCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	70054	72072	2134-2145	1.000000	70.8
clpP_p4	clpP	4	TCACGAATTGACCCGGAATC	AGCCTTTGTTACGTTTCCACA	20	21	50.0	42.9	58.0	58.4	0.7	0.0	1.372740	70054	72072	2112-2123	1.000000	70.0
clpP_p5	clpP	5	TCACGAATTGACCCGGAATC	AGCCTTTGTTACGTTTCCAC	20	20	50.0	45.0	58.0	56.6	0.7	0.0	1.453363	70054	72072	2112-2123	1.000000	69.3
rpl14-rpl16_p1	rpl14-rpl16	1	ACACATCAATTCTCGAGCCC	AATAGCAGCGTCCAAAATGC	20	20	50.0	45.0	58.0	57.4	7.8	0.0	0.596790	81158	81290	240-252	1.000000	80.1
rpl14-rpl16_p2	rpl14-rpl16	2	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	20	20	50.0	45.0	58.0	57.4	17.2	0.0	0.603780	81158	81290	270-282	1.000000	79.5
rpl14-rpl16_p3	rpl14-rpl16	3	ACACATCAATTCTCGAGCCC	GCGTCCAAAATGCCTATACG	20	20	50.0	50.0	58.0	57.3	7.8	0.0	0.693117	81158	81290	233-245	1.000000	79.5
rpl14-rpl16_p4	rpl14-rpl16	4	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	20	20	50.0	50.0	58.0	57.3	17.2	0.0	0.700107	81158	81290	263-275	1.000000	78.8
rpl14-rpl16_p5	rpl14-rpl16	5	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	20	20	50.0	45.0	57.5	57.4	1.8	0.0	1.100218	81158	81290	222-234	1.000000	76.4
rpl16-rps3_p1	rpl16-rps3	1	AAACCAACGAGTCACACACT	GAGGGTAGGGTTCCACTACA	20	20	45.0	55.0	57.6	57.8	0.0	6.0	0.639472	82742	82895	280-302	1.000000	80.0
rpl16-rps3_p2	rpl16-rps3	2	AAACCAACGAGTCACACACT	AGAGGGTAGGGTTCCACTAC	20	20	45.0	55.0	57.6	57.5	0.0	0.0	0.943242	82742	82895	281-303	1.000000	77.5
rpl16-rps3_p3	rpl16-rps3	3	AAACCAACGAGTCACACACT	AGGGTAGGGTTCCACTACAA	20	20	45.0	50.0	57.6	57.3	0.0	6.0	1.124019	82742	82895	279-301	1.000000	76.1
rpl16-rps3_p4	rpl16-rps3	4	AACGAGTCACACACTAAGCA	GAGGGTAGGGTTCCACTACA	20	20	45.0	55.0	57.1	57.8	0.0	6.0	1.125809	82742	82895	275-297	1.000000	76.1
rpl16-rps3_p5	rpl16-rps3	5	AAACCAACGAGTCACACACT	GAGAGGGTAGGGTTCCACTA	20	20	45.0	55.0	57.6	57.2	0.0	0.0	1.239235	82742	82895	282-304	1.000000	75.2
ycf1_p1	ycf1	1	CACCAGCTTCGAACAAGAA	GCGCATAGCTCCTTTGACTA	19	20	47.4	50.0	55.8	57.8	0.0	0.3	3.445512	110674	110793	180-180	1.000000	56.4
ycf1_p2	ycf1	2	CACCAGCTTCGAACAAGAA	ATGGCTCGACGAAAATCTGG	19	20	47.4	50.0	55.8	58.3	0.0	0.0	3.563791	110674	110793	161-161	1.000000	56.0
ycf1_p3	ycf1	3	CACCAGCTTCGAACAAGAA	GCTCCTTTGACTATGGCTCG	19	20	47.4	55.0	55.8	58.4	0.0	0.0	3.644088	110674	110793	173-173	1.000000	55.0
ycf1_p4	ycf1	4	CACCAGCTTCGAACAAGAA	CCTTTGACTATGGCTCGACG	19	20	47.4	55.0	55.8	58.4	0.0	0.0	3.659520	110674	110793	170-170	1.000000	55.0
ycf1_p5	ycf1	5	CACCAGCTTCGAACAAGAA	CTTTGACTATGGCTCGACGA	19	20	47.4	50.0	55.8	57.4	0.0	0.0	3.782177	110674	110793	169-169	1.000000	54.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	123672	124530	989-992	1.000000	78.5
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	123672	124530	990-993	1.000000	78.5
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	123672	124530	972-975	1.000000	78.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	123672	124530	973-976	1.000000	78.3
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.489250	123672	124530	999-1002	1.000000	77.5