primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	122	718	647-666	1.000000	87.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	122	718	661-680	1.000000	87.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	122	718	660-679	1.000000	87.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	122	718	662-681	1.000000	87.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	122	718	696-715	1.000000	85.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCGATGGTATGGACGAATC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4626	5713	1261-1288	1.000000	81.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4626	5713	1260-1287	1.000000	81.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4626	5713	1199-1226	1.000000	81.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4626	5713	1198-1225	1.000000	81.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4626	5713	1208-1235	1.000000	80.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	ATTCGGAATGAAAGCGTCCA	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.9	57.9	1.7	0.0	0.208555	11013	11198	311-358	1.000000	84.4
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TCGGAATGAAAGCGTCCATT	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.208555	11013	11198	309-356	1.000000	84.4
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTCGGAATGAAAGCGTCCAT	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.208555	11013	11198	310-357	1.000000	84.4
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	CGGAATGAAAGCGTCCATTG	AAGACATTCACCGAGGAAGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.279079	11013	11198	308-355	1.000000	83.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TCGGAATGAAAGCGTCCATT	TCAGGAACAATTGGAACGCT	20	20	45.0	45.0	57.9	57.7	0.0	10.8	0.378302	11013	11198	265-312	1.000000	83.4
atpA-atpF_p1	atpA-atpF	1	CCGTCGCCTACTTGAAGTAC	TGTTTGGGTCGCTGAAAGAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.140112	12723	12778	200-201	1.000000	81.3
atpA-atpF_p2	atpA-atpF	2	GTCGCCTACTTGAAGTACGG	TGTTTGGGTCGCTGAAAGAA	20	20	55.0	45.0	58.1	58.0	13.2	0.0	0.140112	12723	12778	198-199	1.000000	81.3
atpA-atpF_p3	atpA-atpF	3	CCGTCGCCTACTTGAAGTAC	ATGTTTGGGTCGCTGAAAGA	20	20	55.0	45.0	58.1	57.7	0.0	0.0	0.364903	12723	12778	201-202	1.000000	79.5
atpA-atpF_p4	atpA-atpF	4	GTCGCCTACTTGAAGTACGG	ATGTTTGGGTCGCTGAAAGA	20	20	55.0	45.0	58.1	57.7	13.2	0.0	0.364903	12723	12778	199-200	1.000000	79.5
atpA-atpF_p5	atpA-atpF	5	GTCGCCTACTTGAAGTACGG	CATGTTTGGGTCGCTGAAAG	20	20	55.0	50.0	58.1	57.7	13.2	0.0	0.431105	12723	12778	200-201	1.000000	78.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48904	49950	1087-1106	1.000000	83.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48904	49950	1095-1114	1.000000	82.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48904	49950	1119-1138	1.000000	82.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48904	49950	1127-1146	1.000000	81.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	48904	49950	1101-1120	1.000000	81.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCTGGCCCTTTGCTAATTGG	GCACGTTGGGTCTTTGAAAG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.268368	53198	54123	1065-1139	1.000000	82.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCTGGCCCTTTGCTAATTGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.345979	53198	54123	1001-1075	1.000000	81.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCTGGCCCTTTGCTAATTGG	AATGCACGTTGGGTCTTTGA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.462341	53198	54123	1068-1142	1.000000	80.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTGGCCCTTTGCTAATTGG	ATGCACGTTGGGTCTTTGAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.462341	53198	54123	1067-1141	1.000000	80.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTGCTAATTGGGGCTA	GCACGTTGGGTCTTTGAAAG	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.572272	53198	54123	1060-1134	1.000000	80.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	56.3	6.4	12.5	1.763328	67523	68950	1567-1579	1.000000	67.2
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	67523	68950	1490-1502	1.000000	66.4
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.883014	67523	68950	1576-1588	1.000000	66.3
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	58.2	56.3	0.0	12.5	1.895919	67523	68950	1581-1593	1.000000	66.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	0.0	12.5	1.899345	67523	68950	1575-1587	1.000000	66.1
rps12-clpP_p1	rps12-clpP	1	CCCCAAGAGCAGGAGATTTC	TATAAAGCAACGGGGCCATC	20	20	55.0	50.0	58.0	58.0	0.0	4.4	0.074229	73043	73302	752-826	1.000000	87.5
rps12-clpP_p2	rps12-clpP	2	TGACCCTGTTAGTCCGTTCT	GAATTCCATTGGAGAGCCGT	20	20	50.0	50.0	58.0	58.0	0.0	5.4	0.049493	73043	73302	973-1047	1.000000	86.7
rps12-clpP_p3	rps12-clpP	3	TGACCCTGTTAGTCCGTTCT	TATAAAGCAACGGGGCCATC	20	20	50.0	50.0	58.0	58.0	0.0	4.4	0.029711	73043	73302	1114-1188	1.000000	86.6
rps12-clpP_p4	rps12-clpP	4	TGACCCTGTTAGTCCGTTCT	ACGAACAGACAAGCCCTTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.058152	73043	73302	810-810	0.333333	52.9
rps12-clpP_p5	rps12-clpP	5	TGACCCTGTTAGTCCGTTCT	ACTACTGAAACTGCGCGAAT	20	20	50.0	45.0	58.0	57.9	0.0	12.1	0.085656	73043	73302	852-852	0.333333	52.6
clpP_p1	clpP	1	TGACCCTGTTAGTCCGTTCT	GCCATTAGTACGCTTCCACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023912	73303	75498	2732-2947	0.666667	73.3
clpP_p2	clpP	2	CCCCAAGAGCAGGAGATTTC	GCCATTAGTACGCTTCCACA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.068430	73303	75498	2370-2585	0.666667	73.3
clpP_p3	clpP	3	CTGATTGGCTGTCTTGGGTT	GCCATTAGTACGCTTCCACA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103401	73303	75498	2341-2556	0.666667	73.1
clpP_p4	clpP	4	ATGAAGCTTGGTTGGGTAGC	GCCATTAGTACGCTTCCACA	20	20	50.0	50.0	58.2	58.0	2.1	0.0	0.179118	73303	75498	2752-2967	0.666667	72.1
clpP_p5	clpP	5	CCTGTTAGTCCGTTCTTGCA	GCCATTAGTACGCTTCCACA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.182360	73303	75498	2728-2943	0.666667	72.1
ycf1_p1	ycf1	1	ATTTCAAGTTGCCGAGTGGT	AACCAAACCCACGGATTTGA	20	20	45.0	45.0	58.0	57.9	0.0	4.3	0.093472	115436	116435	2031-2031	0.333333	52.6
ycf1_p2	ycf1	2	CTCTGGGAGCAGTTTCCATC	AACCAAACCCACGGATTTGA	20	20	55.0	45.0	58.0	57.9	0.0	4.3	0.110259	115436	116435	2150-2150	0.333333	52.5
ycf1_p3	ycf1	3	AGCTAAAAATATCGCCCGCA	AACCAAACCCACGGATTTGA	20	20	45.0	45.0	58.0	57.9	0.0	4.3	0.112935	115436	116435	2058-2058	0.333333	52.4
ycf1_p4	ycf1	4	ATTTCAAGTTGCCGAGTGGT	GAAGAACCAAACCCACGGAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.113106	115436	116435	2035-2035	0.333333	52.4
ycf1_p5	ycf1	5	CTCTGGGAGCAGTTTCCATC	GAAGAACCAAACCCACGGAT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.129893	115436	116435	2154-2154	0.333333	52.3
ndhF-rpl32_p1	ndhF-rpl32	1	ACATAGGAACCGGAAGTGGA	ACGAATACGCTTCTTGGAGA	20	20	50.0	45.0	58.1	56.7	9.5	0.0	1.372175	120853	121922	749-1179	1.000000	80.4
ndhF-rpl32_p2	ndhF-rpl32	2	AACCGGAAGTGGAACGAAAG	ACGAATACGCTTCTTGGAGA	20	20	50.0	45.0	58.4	56.7	9.5	0.0	1.729357	120853	121922	742-1172	1.000000	77.6
ndhF-rpl32_p3	ndhF-rpl32	3	GAACCGGAAGTGGAACGAAA	ACGAATACGCTTCTTGGAGA	20	20	50.0	45.0	58.4	56.7	9.5	0.0	1.733862	120853	121922	743-1173	1.000000	77.6
ndhF-rpl32_p4	ndhF-rpl32	4	CGGAAGTGGAACGAAAGGTA	ACGAATACGCTTCTTGGAGA	20	20	50.0	45.0	57.6	56.7	0.0	0.0	1.763047	120853	121922	739-1169	1.000000	77.4
ndhF-rpl32_p5	ndhF-rpl32	5	ACATAGGAACCGGAAGTGGA	ACGAATACGCTTCTTGGAGAT	20	21	50.0	42.9	58.1	57.2	9.5	0.0	1.855964	120853	121922	749-1179	1.000000	76.5
ycf1_p1	ycf1	1	CCATACTGAAACGACTGCCA	AAAAGTATGCGGGATCGGAC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.143841	133599	133718	735-807	1.000000	90.4
ycf1_p2	ycf1	2	ACCATACTGAAACGACTGCC	AAAAGTATGCGGGATCGGAC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.145297	133599	133718	736-808	1.000000	90.4
ycf1_p3	ycf1	3	CCATACTGAAACGACTGCCA	TCAAATCCGTGGGTTTGGTT	20	20	50.0	45.0	57.9	57.9	0.0	21.3	0.168035	133599	133718	1042-1096	1.000000	88.9
ycf1_p4	ycf1	4	ACCATACTGAAACGACTGCC	TCAAATCCGTGGGTTTGGTT	20	20	50.0	45.0	57.9	57.9	0.0	21.3	0.169491	133599	133718	1043-1097	1.000000	88.9
ycf1_p5	ycf1	5	CCATACTGAAACGACTGCCA	ATCCGTGGGTTTGGTTCTTC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.187669	133599	133718	1038-1092	1.000000	88.8