primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	syfB	1	GAATCCTCCCACCCCAGATA	ACAAAATCCGGCTTATGGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.262279	14301	16346	2633-2639	1.000000	85.4
syfB_p2	syfB	2	GGGTGTAGCCAAGTGGTAAG	ACAAAATCCGGCTTATGGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.253300	14301	16346	2686-2692	1.000000	85.3
syfB_p3	syfB	3	GGTGTAGCCAAGTGGTAAGG	ACAAAATCCGGCTTATGGCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.253300	14301	16346	2685-2691	1.000000	85.3
syfB_p4	syfB	4	GAATCCTCCCACCCCAGATA	AGGAAAGTCCGGGCTCTAAT	20	20	55.0	50.0	57.9	58.1	0.0	10.2	0.237463	14301	16346	2960-2965	1.000000	85.1
syfB_p5	syfB	5	GGTGTAGCCAAGTGGTAAGG	TCCGGCTTATGGCTTACTCT	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.319902	14301	16346	2679-2685	1.000000	84.8
moeB_p1	moeB	1	GAAGGACTTGAACCCTCGAC	ACTTGGTACGACCTGAAGAAG	20	21	55.0	47.6	57.9	57.3	0.0	0.0	1.743972	22528	23610	1738-1738	0.500000	46.0
moeB_p2	moeB	2	GAAGGACTTGAACCCTCGAC	CTTGGTACGACCTGAAGAAGT	20	21	55.0	47.6	57.9	57.3	0.0	0.0	1.743972	22528	23610	1737-1737	0.500000	46.0
moeB_p3	moeB	3	GAGTTGCCTTACCAGCCATT	ACTTGGTACGACCTGAAGAAG	20	21	50.0	47.6	58.2	57.3	0.0	0.0	1.822823	22528	23610	1975-1975	0.500000	45.4
moeB_p4	moeB	4	GAGTTGCCTTACCAGCCATT	CTTGGTACGACCTGAAGAAGT	20	21	50.0	47.6	58.2	57.3	0.0	0.0	1.822823	22528	23610	1974-1974	0.500000	45.4
moeB_p5	moeB	5	TACACGCCTTGAAGGACTTG	CTTGGTACGACCTGAAGAAGT	20	21	50.0	47.6	57.8	57.3	16.9	0.0	1.826064	22528	23610	1747-1747	0.500000	45.4
rpl19_p1	rpl19	1	CTACAGCGACAAAGCCAGAA	AGTGGTGATGCAAGCTTTCA	20	20	50.0	45.0	58.2	58.0	0.0	0.1	0.218759	28872	29225	1478-1483	1.000000	85.1
rpl19_p2	rpl19	2	CTACAGCGACAAAGCCAGAA	TCAGTGGTGATGCAAGCTTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.218759	28872	29225	1480-1485	1.000000	85.1
rpl19_p3	rpl19	3	TCAGCATTATCGCCAAGCAT	AGTGGTGATGCAAGCTTTCA	20	20	45.0	45.0	58.0	58.0	0.0	0.1	0.043046	28872	29225	1694-1694	0.500000	59.7
rpl19_p4	rpl19	4	TCAGCATTATCGCCAAGCAT	TCAGTGGTGATGCAAGCTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.043046	28872	29225	1696-1696	0.500000	59.7
rpl19_p5	rpl19	5	TCAGCATTATCGCCAAGCAT	TGGTTGCACCAGGAAAAAGT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.169217	28872	29225	1369-1369	0.500000	58.6
rpl19-QQR83_pgt008_p1	rpl19-QQR83_pgt008	1	AGCTTCTTCTTGTGCAAGCA	TCAGTGGTGATGCAAGCTTT	20	20	45.0	45.0	58.3	58.0	7.0	0.0	0.330584	29226	29442	1311-1316	1.000000	84.7
rpl19-QQR83_pgt008_p2	rpl19-QQR83_pgt008	2	AGCTTCTTCTTGTGCAAGCA	AGTGGTGATGCAAGCTTTCA	20	20	45.0	45.0	58.3	58.0	7.0	0.1	0.330584	29226	29442	1309-1314	1.000000	84.6
rpl19-QQR83_pgt008_p3	rpl19-QQR83_pgt008	3	AGCTTCTTCTTGTGCAAGCA	TGGTTGCACCAGGAAAAAGT	20	20	45.0	45.0	58.3	58.1	7.0	0.0	0.456755	29226	29442	984-989	1.000000	84.6
rpl19-QQR83_pgt008_p4	rpl19-QQR83_pgt008	4	AGCTTCTTCTTGTGCAAGCA	TACCGGCTGCAATAGGAGTA	20	20	45.0	50.0	58.3	57.9	7.0	0.0	0.392264	29226	29442	1365-1370	1.000000	84.1
rpl19-QQR83_pgt008_p5	rpl19-QQR83_pgt008	5	AGCTTCTTCTTGTGCAAGCA	TGCTGGCTTAGGAACAATGG	20	20	45.0	50.0	58.3	58.2	7.0	0.0	0.477945	29226	29442	1394-1399	1.000000	83.4
rps10-tufA_p1	rps10-tufA	1	AGATGTTGCAGAAGACGAGG	TGTTTCTGGTTCAGCCCTTC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.395551	39283	39302	1337-1337	0.500000	56.8
rps10-tufA_p2	rps10-tufA	2	AGATGTTGCAGAAGACGAGG	AACTGTTGGAGCAGGAGTTG	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.403936	39283	39302	656-656	0.500000	56.8
rps10-tufA_p3	rps10-tufA	3	AGATGTTGCAGAAGACGAGG	CTGTTGGAGCAGGAGTTGTT	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.403936	39283	39302	654-654	0.500000	56.8
rps10-tufA_p4	rps10-tufA	4	AGATGTTGCAGAAGACGAGG	ACTGTTGCAACTGGTCGAAT	20	20	50.0	45.0	57.6	58.0	0.0	31.8	0.409489	39283	39302	1141-1141	0.500000	56.7
rps10-tufA_p5	rps10-tufA	5	AGATGTTGCAGAAGACGAGG	GGAGGAAGAACTGTTGGAGC	20	20	50.0	55.0	57.6	58.2	0.0	0.0	0.562015	39283	39302	664-664	0.500000	55.5
infB_p1	infB	1	ACCTTCTGATTGTGCTGACA	AATGCCGAGACGTTCTACAG	20	20	45.0	50.0	57.1	57.7	0.0	0.0	1.216502	82044	84329	2715-2715	1.000000	76.6
infB_p2	infB	2	GTGCTGACATTCCATCTCTGT	AATGCCGAGACGTTCTACAG	21	20	47.6	50.0	58.0	57.7	0.0	0.0	1.296354	82044	84329	2704-2704	1.000000	76.0
infB_p3	infB	3	TGTGCTGACATTCCATCTCTG	AATGCCGAGACGTTCTACAG	21	20	47.6	50.0	58.0	57.7	0.0	0.0	1.297685	82044	84329	2705-2705	1.000000	75.9
infB_p4	infB	4	TCACCTTCTGATTGTGCTGA	AATGCCGAGACGTTCTACAG	20	20	45.0	50.0	56.8	57.7	3.1	0.0	1.507836	82044	84329	2717-2717	1.000000	74.2
infB_p5	infB	5	TCACCTTCTGATTGTGCTGAC	AATGCCGAGACGTTCTACAG	21	20	47.6	50.0	58.2	57.7	3.1	0.0	1.508493	82044	84329	2717-2717	1.000000	74.2
QQR83_pgp086_p1	QQR83_pgp086	1	ACCAACTTCAGCAAGCTCTT	TGCGTATACCAATTTCGCCA	20	20	45.0	45.0	57.6	58.0	0.0	0.0	0.380598	103059	103838	1435-1435	1.000000	82.9
QQR83_pgp086_p2	QQR83_pgp086	2	ACCAACTTCAGCAAGCTCTT	CCAATTTCGCCATCTACCCA	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.406344	103059	103838	1427-1427	1.000000	82.7
QQR83_pgp086_p3	QQR83_pgp086	3	ACCAACTTCAGCAAGCTCTT	ACCAATTTCGCCATCTACCC	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.407792	103059	103838	1428-1428	1.000000	82.7
QQR83_pgp086_p4	QQR83_pgp086	4	ACCAACTTCAGCAAGCTCTT	TGCTCGTCTTACAATCTGCC	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.365155	103059	103838	1603-1603	0.500000	57.1
QQR83_pgp086_p5	QQR83_pgp086	5	GCTGCAACAACTGACCCTAA	TGCTCGTCTTACAATCTGCC	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.405541	103059	103838	1683-1683	0.500000	56.8
orf621_p1	orf621	1	CCAAGTTCCTTCAATGTCATGC	CCAGCCATGTGGAGAATGAA	22	20	45.5	50.0	58.2	57.9	0.0	2.6	2.291638	106086	108002	2720-2720	0.500000	41.7
orf621_p2	orf621	2	CAAGTTCCTTCAATGTCATGCA	CCAGCCATGTGGAGAATGAA	22	20	40.9	50.0	57.5	57.9	0.0	2.6	2.641743	106086	108002	2719-2719	0.500000	38.9
orf621_p3	orf621	3	CCAAGTTCCTTCAATGTCATGC	GAACGCGGTATAACTCCTGT	22	20	45.5	50.0	58.2	57.4	0.0	12.5	2.732298	106086	108002	2131-2131	0.500000	38.1
orf621_p4	orf621	4	AGTTCCTTCAATGTCATGCA	CCAGCCATGTGGAGAATGAA	20	20	40.0	50.0	55.2	57.9	0.0	2.6	2.890363	106086	108002	2717-2717	0.500000	36.9
orf621_p5	orf621	5	CCAAGTTCCTTCAATGTCATGC	AGAACGCGGTATAACTCCTG	22	20	45.5	50.0	58.2	57.1	0.0	12.5	3.023699	106086	108002	2132-2132	0.500000	35.8
QQR83_pgt016_p1	QQR83_pgt016	1	CCACATGGCTGGGAAGTTAT	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.491027	108977	110739	2436-2439	1.000000	82.4
QQR83_pgt016_p2	QQR83_pgt016	2	TTCATTCTCCACATGGCTGG	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.182072	108977	110739	2447-2447	0.500000	58.5
QQR83_pgt016_p3	QQR83_pgt016	3	ATCTCTCCATCTGCTGTTGC	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.377610	108977	110739	2292-2292	0.500000	57.0
QQR83_pgt016_p4	QQR83_pgt016	4	TTCATTCTCCACATGGCTGG	TTCCAAGCTAATGATGCGGG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.454142	108977	110739	2064-2064	0.500000	56.4
QQR83_pgt016_p5	QQR83_pgt016	5	TTCATTCTCCACATGGCTGG	ATTCCCGCTACCCGCTTATA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.497133	108977	110739	2040-2040	0.500000	56.0
orf365_p1	orf365	1	ATATTGCATCTGTGGCCGAG	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.160576	109518	110615	2208-2208	0.500000	58.7
orf365_p2	orf365	2	ATATTGCATCTGTGGCCGAG	TATGCATCTGGCTGGATTCG	20	20	50.0	50.0	58.1	57.8	0.0	0.2	0.286149	109518	110615	1770-1770	0.500000	57.7
orf365_p3	orf365	3	ATATTGCATCTGTGGCCGAG	CCTTTTCAGGATGGCGGATT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.344026	109518	110615	1738-1738	0.500000	57.2
orf365_p4	orf365	4	ATCTCTCCATCTGCTGTTGC	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.377610	109518	110615	2292-2292	0.500000	57.0
orf365_p5	orf365	5	ATATTGCATCTGTGGCCGAG	TTCCAAGCTAATGATGCGGG	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.432646	109518	110615	1825-1825	0.500000	56.5
QQR83_pgt016-QQR83_pgt017_p1	QQR83_pgt016-QQR83_pgt017	1	AATCCGCCATCCTGAAAAGG	TTCCAAGCTAATGATGCGGG	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.553102	110740	110756	107-107	1.000000	77.8
QQR83_pgt016-QQR83_pgt017_p2	QQR83_pgt016-QQR83_pgt017	2	AATCCGCCATCCTGAAAAGG	GCTAATGATGCGGGTTCGAT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.719315	110740	110756	101-101	1.000000	76.6
QQR83_pgt016-QQR83_pgt017_p3	QQR83_pgt016-QQR83_pgt017	3	AATCCGCCATCCTGAAAAGG	CCAAGCTAATGATGCGGGTT	20	20	50.0	50.0	58.2	58.6	0.0	0.0	0.839652	110740	110756	105-105	1.000000	75.6
QQR83_pgt016-QQR83_pgt017_p4	QQR83_pgt016-QQR83_pgt017	4	CCATCCTGAAAAGGACACCG	TTCCAAGCTAATGATGCGGG	20	20	55.0	50.0	58.5	58.3	0.0	0.0	0.867706	110740	110756	101-101	1.000000	75.4
QQR83_pgt016-QQR83_pgt017_p5	QQR83_pgt016-QQR83_pgt017	5	AATCCGCCATCCTGAAAAGG	AATGATGCGGGTTCGATTCC	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.923243	110740	110756	98-98	1.000000	75.1
QQR83_pgt017-ycf4_p1	QQR83_pgt017-ycf4	1	AATCCGCCATCCTGAAAAGG	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.281032	110828	110877	487-490	1.000000	83.0
QQR83_pgt017-ycf4_p2	QQR83_pgt017-ycf4	2	CCCGCATCATTAGCTTGGAA	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.369652	110828	110877	400-403	1.000000	82.8
QQR83_pgt017-ycf4_p3	QQR83_pgt017-ycf4	3	ATCGAACCCGCATCATTAGC	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.535865	110828	110877	406-409	1.000000	81.4
QQR83_pgt017-ycf4_p4	QQR83_pgt017-ycf4	4	CCATCCTGAAAAGGACACCG	TGTGGAATGTAGGCTCTGGA	20	20	55.0	50.0	58.5	58.0	0.0	0.0	0.595636	110828	110877	481-484	1.000000	80.5
QQR83_pgt017-ycf4_p5	QQR83_pgt017-ycf4	5	CGAATCCAGCCAGATGCATA	TGTGGAATGTAGGCTCTGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.223154	110828	110877	458-458	0.500000	58.2
ycf23_p1	ycf23	1	ACGTTGTGAAACTCTTGTCG	GGTTGTAAGCGCTGTGAAAC	20	20	45.0	50.0	56.6	58.0	0.0	12.6	1.399311	127732	128574	1566-1567	1.000000	74.8
ycf23_p2	ycf23	2	CGTTGTGAAACTCTTGTCGT	GGTTGTAAGCGCTGTGAAAC	20	20	45.0	50.0	56.6	58.0	0.0	12.6	1.399311	127732	128574	1565-1566	1.000000	74.8
ycf23_p3	ycf23	3	ACGTTGTGAAACTCTTGTCGT	GGTTGTAAGCGCTGTGAAAC	21	20	42.9	50.0	58.4	58.0	0.0	12.6	1.418723	127732	128574	1566-1567	1.000000	74.7
ycf23_p4	ycf23	4	CGTTGTGAAACTCTTGTCGT	ATGGGATGGCTGTAAAGCTG	20	20	45.0	50.0	56.6	57.9	0.0	4.5	1.429867	127732	128574	1624-1624	0.500000	48.6
ycf23_p5	ycf23	5	CGTTGTGAAACTCTTGTCGT	CATGGGATGGCTGTAAAGCT	20	20	45.0	50.0	56.6	57.9	0.0	4.5	1.429867	127732	128574	1625-1625	0.500000	48.6
pgmA_p1	pgmA	1	GCAATTGCATCAGCTGACAG	TGGCTTCTTTGATGACTGCA	20	20	50.0	45.0	58.1	57.7	25.4	0.0	0.365758	135490	137058	2916-2916	0.500000	57.1
pgmA_p2	pgmA	2	TGCATCAGCTGACAGTCAAG	TGGCTTCTTTGATGACTGCA	20	20	50.0	45.0	58.2	57.7	21.0	0.0	0.470533	135490	137058	2911-2911	0.500000	56.2
pgmA_p3	pgmA	3	ACACAAATGCCTGGTCCTAC	TGGCTTCTTTGATGACTGCA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.475507	135490	137058	2739-2739	0.500000	56.2
pgmA_p4	pgmA	4	TTGCATCAGCTGACAGTCAA	TGGCTTCTTTGATGACTGCA	20	20	45.0	45.0	57.7	57.7	21.0	0.0	0.534400	135490	137058	2912-2912	0.500000	55.7
pgmA_p5	pgmA	5	CGTTATCAAAGGGCAACAGC	TGGCTTCTTTGATGACTGCA	20	20	50.0	45.0	57.7	57.7	0.0	0.0	0.537706	135490	137058	2850-2850	0.500000	55.7
trpA_p1	trpA	1	CGTCTAGAGGCCTAGGACAT	GGTCGCTAACTCAACGGTAG	20	20	55.0	55.0	57.7	58.1	19.5	0.0	0.374485	140382	141173	965-973	1.000000	81.9
trpA_p2	trpA	2	CGTCTAGAGGCCTAGGACAT	ATGTTGTCAGGGTCGCTAAC	20	20	55.0	50.0	57.7	57.9	19.5	0.0	0.374697	140382	141173	975-983	1.000000	81.8
trpA_p3	trpA	3	TCTCCCTTTCACGGAGGTAA	GGTCGCTAACTCAACGGTAG	20	20	50.0	55.0	57.7	58.1	4.1	0.0	0.408621	140382	141173	946-954	1.000000	81.7
trpA_p4	trpA	4	TCTCCCTTTCACGGAGGTAA	ATGTTGTCAGGGTCGCTAAC	20	20	50.0	50.0	57.7	57.9	4.1	0.0	0.408833	140382	141173	956-964	1.000000	81.6
trpA_p5	trpA	5	CGTCTAGAGGCCTAGGACAT	ACGGTAGAGTACTCGGCTTT	20	20	55.0	50.0	57.7	58.2	19.5	17.1	0.448354	140382	141173	952-960	1.000000	81.4
tilS_p1	tilS	1	CTACCGTTGAGTTAGCGACC	TGCGGGTCCTACAAAAACAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.148783	141326	142309	1197-1197	1.000000	85.1
tilS_p2	tilS	2	GTTAGCGACCCTGACAACAT	TGCGGGTCCTACAAAAACAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.148994	141326	142309	1187-1187	1.000000	85.1
tilS_p3	tilS	3	GTTAGCGACCCTGACAACAT	TGCGCAAAGAACTGGTACTT	20	20	50.0	45.0	57.9	58.0	0.0	8.2	0.132969	141326	142309	1603-1603	1.000000	85.0
tilS_p4	tilS	4	CTACCGTTGAGTTAGCGACC	TGCGCAAAGAACTGGTACTT	20	20	55.0	45.0	58.1	58.0	0.0	8.2	0.132757	141326	142309	1613-1613	1.000000	84.9
tilS_p5	tilS	5	TCCTTTAGCCCCAGCAAATG	TGCGCAAAGAACTGGTACTT	20	20	50.0	45.0	58.1	58.0	0.0	8.2	0.186903	141326	142309	2061-2061	0.500000	58.5