primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK(uuu)-rps16_p1	trnK(uuu)-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAGGGCGCTCAACCTAC	20	19	50.0	52.6	58.2	58.1	20.3	11.4	1.225778	4208	5010	932-934	1.000000	70.8
trnK(uuu)-rps16_p2	trnK(uuu)-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAGGGCGCTCAACCTAC	20	19	50.0	52.6	58.2	58.1	20.3	11.4	1.225778	4208	5010	931-933	1.000000	70.8
trnK(uuu)-rps16_p3	trnK(uuu)-rps16	3	CCGCACTTAAAAGCCGAGTA	AAAAGGGCGCTCAACCTAC	20	19	50.0	52.6	58.3	58.1	5.3	11.4	1.340165	4208	5010	941-943	1.000000	69.9
trnK(uuu)-rps16_p4	trnK(uuu)-rps16	4	AAAGCCGAGTACTCTACCGT	GAAAAGGGCGCTCAACCTAC	20	20	50.0	55.0	58.2	59.2	20.3	11.4	1.365303	4208	5010	933-935	1.000000	69.7
trnK(uuu)-rps16_p5	trnK(uuu)-rps16	5	AAGCCGAGTACTCTACCGTT	GAAAAGGGCGCTCAACCTAC	20	20	50.0	55.0	58.2	59.2	20.3	11.4	1.365303	4208	5010	932-934	1.000000	69.7
atpA-atpF_p1	atpA-atpF	1	CCGTCGCCTACTTGAAGTAC	CAGCGCTAATATTGGCATGC	20	20	55.0	50.0	58.1	58.0	0.0	21.8	0.121412	12096	12162	227-229	1.000000	79.7
atpA-atpF_p2	atpA-atpF	2	CCGTCGCCTACTTGAAGTAC	AGCGCTAATATTGGCATGCT	20	20	55.0	45.0	58.1	58.1	0.0	21.8	0.192960	12096	12162	226-228	1.000000	79.2
atpA-atpF_p3	atpA-atpF	3	CCGTCGCCTACTTGAAGTAC	TAATATTGGCATGCTCGGGG	20	20	55.0	50.0	58.1	57.8	0.0	18.9	0.293377	12096	12162	221-223	1.000000	78.4
atpA-atpF_p4	atpA-atpF	4	CCGTCGCCTACTTGAAGTAC	CTCGGGGCCATGAAAGAAAT	20	20	55.0	50.0	58.1	58.2	0.0	4.4	0.326792	12096	12162	208-210	1.000000	78.2
atpA-atpF_p5	atpA-atpF	5	CCGTCGCCTACTTGAAGTAC	GCGCTAATATTGGCATGCTC	20	20	55.0	50.0	58.1	57.7	0.0	21.8	0.347943	12096	12162	225-227	1.000000	77.9
atpH-atpI_p1	atpH-atpI	1	GCAGAAATCAGTGGATTCAT	TTTTTGCAACTTTAGCCGCG	20	20	40.0	45.0	53.6	58.5	10.2	0.0	4.881619	14140	15238	1158-1175	1.000000	45.8
atpH-atpI_p2	atpH-atpI	2	GCAGAAATCAGTGGATTCAT	CAAGCGGGATTCAAGCTCTT	20	20	40.0	50.0	53.6	58.5	10.2	5.7	4.906550	14140	15238	1179-1196	1.000000	45.5
atpH-atpI_p3	atpH-atpI	3	GCAGAAATCAGTGGATTCAT	GGTGAATCCATGGAAGGTCA	20	20	40.0	50.0	53.6	57.2	10.2	20.7	5.170841	14140	15238	1129-1146	1.000000	45.0
atpH-atpI_p4	atpH-atpI	4	GCAGAAATCAGTGGATTCAT	TTAGCCGCGGCCTATATAGG	20	20	40.0	55.0	53.6	58.9	10.2	44.5	5.255120	14140	15238	1147-1164	1.000000	44.9
atpH-atpI_p5	atpH-atpI	5	GCAGAAATCAGTGGATTCAT	TTTAGCCGCGGCCTATATAG	20	20	40.0	50.0	53.6	57.0	10.2	44.5	5.317362	14140	15238	1148-1165	1.000000	44.9
trnT(ggu)-psbD_p1	trnT(ggu)-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	20	20	55.0	55.0	58.1	57.9	7.6	0.0	0.169074	32613	34094	1581-1624	1.000000	83.9
trnT(ggu)-psbD_p2	trnT(ggu)-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.191228	32613	34094	1584-1627	1.000000	83.7
trnT(ggu)-psbD_p3	trnT(ggu)-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32613	34094	1589-1632	1.000000	82.8
trnT(ggu)-psbD_p4	trnT(ggu)-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	32613	34094	1592-1635	1.000000	82.6
trnT(ggu)-psbD_p5	trnT(ggu)-psbD	5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	20	20	55.0	55.0	58.1	58.3	7.6	0.0	0.345672	32613	34094	1582-1625	1.000000	82.5
psbC-trnS(uga)_p1	psbC-trnS(uga)	1	TCCTATTCGTGGGCCATTTG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.9	58.1	10.7	0.0	0.110009	36526	36745	413-413	1.000000	80.7
psbC-trnS(uga)_p2	psbC-trnS(uga)	2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36526	36745	327-327	1.000000	80.2
psbC-trnS(uga)_p3	psbC-trnS(uga)	3	TCCTATTCGTGGGCCATTTG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	57.9	58.1	10.7	0.0	0.180254	36526	36745	424-424	1.000000	80.1
psbC-trnS(uga)_p4	psbC-trnS(uga)	4	TCCTATTCGTGGGCCATTTG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.9	58.2	10.7	17.2	0.268263	36526	36745	359-359	1.000000	79.6
psbC-trnS(uga)_p5	psbC-trnS(uga)	5	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	36526	36745	338-338	1.000000	79.6
trnS(uga)-psbZ_p1	trnS(uga)-psbZ	1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	9.5	29.0	1.054924	36839	37050	281-281	1.000000	71.6
trnS(uga)-psbZ_p2	trnS(uga)-psbZ	2	CTATCAACCACTCGGCCATC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	2.2	29.0	1.125169	36839	37050	270-270	1.000000	71.0
trnS(uga)-psbZ_p3	trnS(uga)-psbZ	3	CGAGCAAAAGGAGAGAGAGG	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	57.7	57.0	0.0	29.0	1.288352	36839	37050	346-346	1.000000	69.7
trnS(uga)-psbZ_p4	trnS(uga)-psbZ	4	AGCAAAAGGAGAGAGAGGGA	TGCAAAAACAGCCAATTGGA	20	20	50.0	40.0	57.7	57.0	0.0	29.0	1.330255	36839	37050	344-344	1.000000	69.4
trnS(uga)-psbZ_p5	trnS(uga)-psbZ	5	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGAA	20	21	55.0	38.1	58.1	57.7	9.5	29.0	1.331249	36839	37050	281-281	1.000000	69.4
trnL(uaa)_p1	trnL(uaa)	1	GGGGGTGGAATCACAATGAG	TGGAACCGATGACGAAACAA	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.474288	48465	48911	501-540	1.000000	83.8
trnL(uaa)_p2	trnL(uaa)	2	GGGGGTGGAATCACAATGAG	TTGGAACCGATGACGAAACA	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.474288	48465	48911	502-541	1.000000	83.8
trnL(uaa)_p3	trnL(uaa)	3	GGGGGTGGAATCACAATGAG	ACCGATGACGAAACAAGGTT	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.482085	48465	48911	497-536	1.000000	83.8
trnL(uaa)_p4	trnL(uaa)	4	GGGGGTGGAATCACAATGAG	AACCGATGACGAAACAAGGT	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.482085	48465	48911	498-537	1.000000	83.7
trnL(uaa)_p5	trnL(uaa)	5	TGAATTCAACGGTTCCGACA	TGGAACCGATGACGAAACAA	20	20	45.0	45.0	57.8	57.8	18.3	0.0	0.484093	48465	48911	537-576	1.000000	83.5
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	64413	65604	1330-1333	1.000000	72.9
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	64413	65604	1379-1382	1.000000	69.7
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	64413	65604	1378-1381	1.000000	69.7
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	20	21	45.0	47.6	58.2	57.5	0.0	4.1	1.673144	64413	65604	1337-1340	1.000000	67.9
petA-psbJ_p5	petA-psbJ	5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	20	21	45.0	47.6	58.2	58.5	0.0	0.0	1.703141	64413	65604	1379-1382	1.000000	67.6
rpl20-rps12_p1	rpl20-rps12	1	AATTTTTGTTCGACGTCGCC	GAAATCCCCTGCTCTTCGAG	20	20	45.0	55.0	58.0	58.1	32.2	0.0	0.080170	70576	71345	879-881	1.000000	79.7
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	GAAATCCCCTGCTCTTCGAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.167115	70576	71345	917-919	1.000000	79.0
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	GAAATCCCCTGCTCTTCGAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.167115	70576	71345	916-918	1.000000	79.0
rpl20-rps12_p4	rpl20-rps12	4	AATTTTTGTTCGACGTCGCC	ACAGCCAATCCGAAATGTCA	20	20	45.0	45.0	58.0	57.8	32.2	0.0	0.225742	70576	71345	900-902	1.000000	78.5
rpl20-rps12_p5	rpl20-rps12	5	AATTTTTGTTCGACGTCGCC	ACAAGACAGCCAATCCGAAA	20	20	45.0	45.0	58.0	57.7	32.2	0.0	0.297267	70576	71345	905-907	1.000000	78.0
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	73606	74040	613-613	1.000000	79.5
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	73606	74040	531-531	1.000000	78.5
clpP-psbB_p3	clpP-psbB	3	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.8	57.9	3.6	0.0	0.283669	73606	74040	559-559	1.000000	78.2
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	GTATAGAGCCATCGAACCGG	20	20	50.0	55.0	58.0	57.7	0.0	0.0	0.350249	73606	74040	631-631	1.000000	77.6
clpP-psbB_p5	clpP-psbB	5	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	20	20	45.0	55.0	58.3	57.9	3.6	0.0	0.388281	73606	74040	557-557	1.000000	77.3
petD_p1	petD	1	TCTCCGAAGAGAAGATGGAT	TGAATCTTTAGCACGATCCGT	20	21	45.0	42.9	55.0	57.5	0.0	0.0	4.470621	78089	79291	1335-1335	1.000000	44.5
petD_p2	petD	2	TCTCCGAAGAGAAGATGGATT	TGAATCTTTAGCACGATCCGT	21	21	42.9	42.9	55.9	57.5	0.0	0.0	4.642823	78089	79291	1335-1335	1.000000	43.2
petD_p3	petD	3	TCTCCGAAGAGAAGATGGAT	GGGAGAATTCACTTTGAAGCC	20	21	45.0	47.6	55.0	57.1	0.0	8.4	4.807880	78089	79291	1271-1271	1.000000	41.9
petD_p4	petD	4	TCTCCGAAGAGAAGATGGATT	GGGAGAATTCACTTTGAAGCC	21	21	42.9	47.6	55.9	57.1	0.0	8.4	4.980082	78089	79291	1271-1271	1.000000	40.5
petD_p5	petD	5	TCTCCGAAGAGAAGATGGAT	ACTTTGAAGCCACTATTCCCT	20	21	45.0	42.9	55.0	56.9	0.0	0.0	5.049008	78089	79291	1261-1261	1.000000	40.3
trnR(acg)-trnN(guu)_p1	trnR(acg)-trnN(guu)	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	109220	109822	744-748	1.000000	78.8
trnR(acg)-trnN(guu)_p2	trnR(acg)-trnN(guu)	2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.7	58.0	18.7	0.0	0.288158	109220	109822	781-785	1.000000	78.2
trnR(acg)-trnN(guu)_p3	trnR(acg)-trnN(guu)	3	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	20	20	55.0	50.0	57.8	58.1	18.9	18.0	0.336954	109220	109822	714-718	1.000000	77.9
trnR(acg)-trnN(guu)_p4	trnR(acg)-trnN(guu)	4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	20	20	55.0	50.0	57.8	57.8	18.9	15.9	0.366304	109220	109822	708-712	1.000000	77.6
trnR(acg)-trnN(guu)_p5	trnR(acg)-trnN(guu)	5	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	109220	109822	695-699	1.000000	77.6
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTATTGGGCCGGCTATTGTT	20	20	50.0	50.0	58.1	58.0	0.0	28.6	0.144614	118617	118890	458-459	1.000000	79.2
psaC-ndhE_p2	psaC-ndhE	2	CAATACATCTGTGGGGCAGG	CTATTGGGCCGGCTATTGTT	20	20	55.0	50.0	58.3	58.0	0.0	28.6	0.334800	118617	118890	434-435	1.000000	77.7
psaC-ndhE_p3	psaC-ndhE	3	CCAATACATCTGTGGGGCAG	CTATTGGGCCGGCTATTGTT	20	20	55.0	50.0	58.3	58.0	0.0	28.6	0.334800	118617	118890	435-436	1.000000	77.7
psaC-ndhE_p4	psaC-ndhE	4	CAGGCTCGTACACATTGAGT	CTATTGGGCCGGCTATTGTT	20	20	50.0	50.0	57.6	58.0	0.0	28.6	0.389025	118617	118890	418-419	1.000000	77.3
psaC-ndhE_p5	psaC-ndhE	5	AGCTTTACATCCGTCCCAAG	CTATTGGGCCGGCTATTGTT	20	20	50.0	50.0	57.6	58.0	0.0	28.6	0.421995	118617	118890	464-465	1.000000	77.0
ndhG_p1	ndhG	1	AACAATAGCCGGCCCAATAG	TTTGTGGGAACTGTGTCGAG	20	20	50.0	50.0	58.0	58.1	26.1	0.0	0.070639	119476	120006	1632-1632	1.000000	79.9
ndhG_p2	ndhG	2	AACAATAGCCGGCCCAATAG	CAGAGCGTTTTCGTGGTAGA	20	20	50.0	50.0	58.0	57.9	26.1	0.0	0.079069	119476	120006	1800-1800	1.000000	79.8
ndhG_p3	ndhG	3	AACAATAGCCGGCCCAATAG	TCAGAGCGTTTTCGTGGTAG	20	20	50.0	50.0	58.0	57.9	26.1	0.0	0.079069	119476	120006	1801-1801	1.000000	79.8
ndhG_p4	ndhG	4	ACCATGTTTCGGCTTGTGAT	TTTGTGGGAACTGTGTCGAG	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.142196	119476	120006	1472-1472	1.000000	79.4
ndhG_p5	ndhG	5	ACCATGTTTCGGCTTGTGAT	CAGAGCGTTTTCGTGGTAGA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.150626	119476	120006	1640-1640	1.000000	79.3
ycf1_p1	ycf1	1	GGGACAAGGAAGATAGATCCC	GGTTCATACGTAAATGGCGC	21	20	52.4	50.0	56.9	57.6	0.0	0.0	2.438224	127085	127204	276-276	1.000000	61.7
ycf1_p2	ycf1	2	GGGACAAGGAAGATAGATCCC	TATTCCCAGGGTCAATGCAC	21	20	52.4	50.0	56.9	57.6	0.0	5.0	2.495089	127085	127204	197-197	1.000000	61.7
ycf1_p3	ycf1	3	GGGACAAGGAAGATAGATCCC	CCAGGGTCAATGCACAACTT	21	20	52.4	50.0	56.9	58.7	0.0	0.0	2.728503	127085	127204	192-192	1.000000	59.8
ycf1_p4	ycf1	4	GGGACAAGGAAGATAGATCCCA	GGTTCATACGTAAATGGCGC	22	20	50.0	50.0	58.7	57.6	1.4	0.0	3.060195	127085	127204	276-276	1.000000	56.7
ycf1_p5	ycf1	5	TGGGACAAGGAAGATAGATCCC	GGTTCATACGTAAATGGCGC	22	20	50.0	50.0	58.7	57.6	0.0	0.0	3.060195	127085	127204	277-277	1.000000	56.7
trnN(guu)-trnR(acg)_p1	trnN(guu)-trnR(acg)	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	131000	131602	744-748	1.000000	78.8
trnN(guu)-trnR(acg)_p2	trnN(guu)-trnR(acg)	2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	20	20	55.0	55.0	58.0	57.7	0.0	18.7	0.288158	131000	131602	781-785	1.000000	78.2
trnN(guu)-trnR(acg)_p3	trnN(guu)-trnR(acg)	3	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.1	57.8	18.0	18.9	0.336954	131000	131602	714-718	1.000000	77.9
trnN(guu)-trnR(acg)_p4	trnN(guu)-trnR(acg)	4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.8	57.8	15.9	18.9	0.366304	131000	131602	708-712	1.000000	77.6
trnN(guu)-trnR(acg)_p5	trnN(guu)-trnR(acg)	5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	131000	131602	695-699	1.000000	77.6