primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	206	567	431-431	1.000000	78.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	206	567	445-445	1.000000	78.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	206	567	444-444	1.000000	78.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	206	567	446-446	1.000000	78.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	206	567	480-480	1.000000	76.4
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTATGGTCATGGGCAAGTG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.593563	3696	4383	820-820	1.000000	75.3
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTATGGTCATGGGCAAGTG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.6	58.2	0.0	17.1	0.593563	3696	4383	819-819	1.000000	75.3
matK-trnK-UUU_p3	matK-trnK-UUU	3	GGTCATGGGCAAGTGCATAA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.5	58.2	5.5	17.1	0.695162	3696	4383	815-815	1.000000	74.5
matK-trnK-UUU_p4	matK-trnK-UUU	4	GGTCATGGGCAAGTGCATAA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.5	58.2	5.5	17.1	0.695162	3696	4383	814-814	1.000000	74.5
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTATGGTCATGGGCAAGTG	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.6	57.5	0.0	0.0	0.885900	3696	4383	833-833	1.000000	73.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GTCGTGGTCTTCCAAACCTT	GGGTGGGGGTTTTTAAGGAA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.439010	4421	5194	940-940	1.000000	76.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCGTGGTCTTCCAAACCTT	AAGGGTGGGGGTTTTTAAGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.445892	4421	5194	942-942	1.000000	76.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGATGGTATGGACGAATCC	GGGTGGGGGTTTTTAAGGAA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.482468	4421	5194	919-919	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCGTGGTCTTCCAAACCTT	AGGGTGGGGGTTTTTAAGGA	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.488560	4421	5194	941-941	1.000000	76.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGATGGTATGGACGAATCC	AAGGGTGGGGGTTTTTAAGG	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.489350	4421	5194	921-921	1.000000	76.2
atpA-atpF_p1	atpA-atpF	1	ATGCCATCGCCTACTTGAAG	TTGGCAGGTTAGGGTCAATG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.255334	11941	12015	229-239	1.000000	78.3
atpA-atpF_p2	atpA-atpF	2	ATGCCATCGCCTACTTGAAG	TACGTGGAGCCTTAGGAACT	20	20	50.0	50.0	58.0	57.8	0.0	0.7	0.267921	11941	12015	301-311	1.000000	78.1
atpA-atpF_p3	atpA-atpF	3	ATCGCCTACTTGAAGTACGC	TTGGCAGGTTAGGGTCAATG	20	20	50.0	50.0	57.8	57.8	13.9	0.0	0.419683	11941	12015	224-234	1.000000	77.0
atpA-atpF_p4	atpA-atpF	4	ATCGCCTACTTGAAGTACGC	TACGTGGAGCCTTAGGAACT	20	20	50.0	50.0	57.8	57.8	13.9	0.7	0.432269	11941	12015	296-306	1.000000	76.8
atpA-atpF_p5	atpA-atpF	5	CAATGCCATCGCCTACTTGA	TTGGCAGGTTAGGGTCAATG	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.547140	11941	12015	231-241	1.000000	76.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.321560	57223	58543	1455-1466	1.000000	78.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	57223	58543	1441-1452	1.000000	78.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTCCTTTGTTAATCGGGGCT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.0	0.0	0.665332	57223	58543	1454-1465	1.000000	75.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	57223	58543	1440-1451	1.000000	75.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTCCTTTGTTAATCGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.697675	57223	58543	1457-1468	1.000000	75.1
rbcL-accD_p1	rbcL-accD	1	ACTACAGTTCGGTGGAGGAA	CATAAACCACCGTTTTCGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033579	63756	64487	991-991	1.000000	79.9
rbcL-accD_p2	rbcL-accD	2	TGCAATGCACGCAGTTATTG	CATAAACCACCGTTTTCGCC	20	20	45.0	50.0	58.0	58.0	2.7	0.0	0.048312	63756	64487	1303-1303	1.000000	79.7
rbcL-accD_p3	rbcL-accD	3	GAAACCGGCGAAATCAAAGG	CATAAACCACCGTTTTCGCC	20	20	50.0	50.0	58.0	58.0	9.3	0.0	0.051020	63756	64487	1497-1497	1.000000	79.7
rbcL-accD_p4	rbcL-accD	4	ACTACAGTTCGGTGGAGGAA	ACCATCGTTATCTTGCGCAT	20	20	50.0	45.0	58.0	58.0	0.0	12.8	0.051811	63756	64487	1444-1444	1.000000	79.7
rbcL-accD_p5	rbcL-accD	5	TGCAATGCACGCAGTTATTG	ACCATCGTTATCTTGCGCAT	20	20	45.0	45.0	58.0	58.0	2.7	12.8	0.066544	63756	64487	1756-1756	1.000000	79.6
accD_p1	accD	1	ATGCGCAAGATAACGATGGT	CATACATAGTACGCCGGGTG	20	20	45.0	55.0	58.0	57.9	17.1	2.2	0.106767	66347	66466	2452-2668	1.000000	83.9
accD_p2	accD	2	TCGAATGATATCGATGCACAAGA	CGAGCTTGGATCTTTTGTCG	23	20	39.1	50.0	58.1	57.2	8.5	0.0	3.924758	66347	66466	104-866	1.000000	54.3
accD_p3	accD	3	CGAATGATATCGATGCACAAGA	CGAGCTTGGATCTTTTGTCG	22	20	40.9	50.0	56.8	57.2	8.5	0.0	4.032244	66347	66466	103-865	1.000000	53.4
accD_p4	accD	4	TCGAATGATATCGATGCACA	CGAGCTTGGATCTTTTGTCG	20	20	40.0	50.0	54.8	57.2	8.5	0.0	4.021010	66347	66466	776-866	1.000000	52.8
accD_p5	accD	5	TCGAATGATATCGATGCACAAG	CGAGCTTGGATCTTTTGTCG	22	20	40.9	50.0	56.8	57.2	8.5	0.0	4.032244	66347	66466	776-866	1.000000	52.7
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	58.1	57.9	3.3	0.0	0.202190	70222	70932	815-815	1.000000	78.6
ycf4-cemA_p2	ycf4-cemA	2	AAAAAGCTGCTGAATTGGCC	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	57.8	57.9	1.4	0.0	0.280841	70222	70932	829-829	1.000000	77.9
ycf4-cemA_p3	ycf4-cemA	3	AAAGCTGCTGAATTGGCCTA	GAGAGAGAAAGCCACCAAGG	20	20	45.0	55.0	57.8	57.9	1.4	0.0	0.327130	70222	70932	827-827	1.000000	77.6
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	AGAGAGAAAGCCACCAAGGA	20	20	45.0	50.0	58.1	58.3	3.3	0.0	0.361058	70222	70932	814-814	1.000000	77.3
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTGCGTGT	CCGGCATTCCACGAATTAGT	20	20	45.0	50.0	58.1	58.3	3.3	0.0	0.432019	70222	70932	863-863	1.000000	76.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.458445	72788	73389	747-747	1.000000	76.4
petA-psbJ_p2	petA-psbJ	2	ATTACGTGTCCAAGGCCTTG	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.2	57.6	34.6	0.0	0.598842	72788	73389	743-743	1.000000	75.3
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.2	57.6	13.8	0.0	0.598842	72788	73389	744-744	1.000000	75.3
petA-psbJ_p4	petA-psbJ	4	CGTGTCCAAGGCCTTGTATT	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.2	57.6	37.1	0.0	0.598842	72788	73389	739-739	1.000000	75.3
petA-psbJ_p5	petA-psbJ	5	TACGTGTCCAAGGCCTTGTA	ATTCTGGATTGGGTTCGTCC	20	20	50.0	50.0	58.7	57.6	37.1	0.0	1.079971	72788	73389	741-741	1.000000	71.5
rpl20-rps12_p1	rpl20-rps12	1	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	20	20	55.0	50.0	58.2	57.9	22.3	12.9	0.302025	78529	79462	1050-1061	1.000000	78.4
rpl20-rps12_p2	rpl20-rps12	2	CCCCTCGAAAGCTTAATGCA	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	58.2	57.9	0.0	12.9	0.315800	78529	79462	1034-1045	1.000000	78.3
rpl20-rps12_p3	rpl20-rps12	3	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	20	20	50.0	50.0	58.3	57.9	22.3	12.9	0.384764	78529	79462	1049-1060	1.000000	77.8
rpl20-rps12_p4	rpl20-rps12	4	GTAAGTCTTGAATGGGCCCC	ACAGCCAATCAGAAATCGCA	20	20	55.0	45.0	58.2	58.2	22.3	0.0	0.410942	78529	79462	1097-1108	1.000000	77.5
rpl20-rps12_p5	rpl20-rps12	5	GTAAGTCTTGAATGGGCCCC	CAGCCAATCAGAAATCGCAC	20	20	55.0	50.0	58.2	57.8	22.3	0.0	0.420417	78529	79462	1096-1107	1.000000	77.4
petB_p1	petB	1	TCATTTAGAGCCCGGGTTTC	TCCTTCCCCAAGTGATAAATGA	20	22	50.0	40.9	57.6	56.8	24.6	0.0	3.630786	84743	86177	1572-1572	1.000000	51.1
petB_p2	petB	2	GGGAGTTCAATCGCGGAATT	TCCTTCCCCAAGTGATAAATGA	20	22	50.0	40.9	58.6	56.8	6.1	0.0	3.837950	84743	86177	1531-1531	1.000000	49.4
petB_p3	petB	3	GGGTTTCGAGCCCATTCTAT	TCCTTCCCCAAGTGATAAATGA	20	22	50.0	40.9	57.4	56.8	18.8	0.0	3.857793	84743	86177	1559-1559	1.000000	49.2
petB_p4	petB	4	TCATTTAGAGCCCGGGTTTC	TTCCTTCCCCAAGTGATAAATGA	20	23	50.0	39.1	57.6	57.5	24.6	0.0	3.941910	84743	86177	1573-1573	1.000000	48.6
petB_p5	petB	5	ATTTTGGGAGTTCAATCGCG	TCCTTCCCCAAGTGATAAATGA	20	22	45.0	40.9	57.1	56.8	0.0	0.0	4.137143	84743	86177	1536-1536	1.000000	47.0
rps7-rps12_p1	rps7-rps12	1	TTTTTCCTTCTGCAGCACCT	CCCTAGATGCTGTCGGAGTA	20	20	45.0	55.0	57.9	58.0	9.5	0.0	0.089117	107406	108026	703-708	1.000000	79.9
rps7-rps12_p2	rps7-rps12	2	TTTTTCCTTCTGCAGCACCT	AGGATTTACCCGGTGTGAGA	20	20	45.0	50.0	57.9	58.1	9.5	0.0	0.124793	107406	108026	742-747	1.000000	79.5
rps7-rps12_p3	rps7-rps12	3	GTTTTTCCTTCTGCAGCACC	CCCTAGATGCTGTCGGAGTA	20	20	50.0	55.0	57.9	58.0	9.5	0.0	0.157404	107406	108026	704-709	1.000000	79.3
rps7-rps12_p4	rps7-rps12	4	TTTTTCCTTCTGCAGCACCT	GAGGGGGAAGGGTTAAGGAT	20	20	45.0	55.0	57.9	58.1	9.5	0.0	0.163135	107406	108026	757-762	1.000000	79.2
rps7-rps12_p5	rps7-rps12	5	GTTTTTCCTTCTGCAGCACC	AGGATTTACCCGGTGTGAGA	20	20	50.0	50.0	57.9	58.1	9.5	0.0	0.193080	107406	108026	743-748	1.000000	79.0
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AGGATTCGAACCTACCACCA	ATCCACAAAAGAGTCCGAGC	20	20	50.0	50.0	58.1	57.9	15.0	0.0	0.153620	118393	119268	884-1018	1.000000	82.8
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AGGATTCGAACCTACCACCA	CTAGGGAGAGTGTGCTCAGA	20	20	50.0	55.0	58.1	57.9	15.0	0.0	0.189314	118393	119268	905-1039	1.000000	82.5
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AGGATTCGAACCTACCACCA	TCCGAGCAAAACGACTGAAT	20	20	50.0	45.0	58.1	57.8	15.0	0.0	0.216833	118393	119268	871-1005	1.000000	82.3
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	AGGATTCGAACCTACCACCA	AAAGAGTCCGAGCAAAACGA	20	20	50.0	45.0	58.1	57.8	15.0	0.0	0.287315	118393	119268	877-1011	1.000000	81.7
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	AGGATTCGAACCTACCACCA	AGGGAGAGTGTGCTCAGAAA	20	20	50.0	50.0	58.1	58.3	15.0	0.0	0.330982	118393	119268	903-1037	1.000000	81.4
ndhF-rpl32_p1	ndhF-rpl32	1	AGGAAGGGAAAGTGGGATGA	AGCTTTTAATGCTGCCCGAT	20	20	50.0	45.0	57.9	58.2	0.0	4.5	0.291998	123454	124289	964-986	1.000000	79.6
ndhF-rpl32_p2	ndhF-rpl32	2	GGTATGAGCCACCCGTATTG	AGCTTTTAATGCTGCCCGAT	20	20	55.0	45.0	58.1	58.2	0.0	4.5	0.353288	123454	124289	942-964	1.000000	79.1
ndhF-rpl32_p3	ndhF-rpl32	3	AGGAAGGGAAAGTGGGATGA	TAATGCTGCCCGATATCCCT	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.403169	123454	124289	958-980	1.000000	78.7
ndhF-rpl32_p4	ndhF-rpl32	4	AGGAAGGGAAAGTGGGATGA	AAAGCTTTTAATGCTGCCCG	20	20	50.0	45.0	57.9	57.6	0.0	5.7	0.428411	123454	124289	966-988	1.000000	78.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGTATGAGCCACCCGTATTG	TAATGCTGCCCGATATCCCT	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.464460	123454	124289	936-958	1.000000	78.2
ccsA_p1	ccsA	1	AGTAGCGGCATGTCATCTTC	ATGGTTCGTGCCCATTCAC	20	19	50.0	52.6	57.8	58.4	1.4	0.0	1.664391	125554	126513	1139-1144	1.000000	66.7
ccsA_p2	ccsA	2	GAGTAGCGGCATGTCATCTT	ATGGTTCGTGCCCATTCAC	20	19	50.0	52.6	57.8	58.4	1.4	0.0	1.664391	125554	126513	1140-1145	1.000000	66.7
ccsA_p3	ccsA	3	GTAGCGGCATGTCATCTTCT	ATGGTTCGTGCCCATTCAC	20	19	50.0	52.6	57.8	58.4	1.4	0.0	1.664391	125554	126513	1138-1143	1.000000	66.7
ccsA_p4	ccsA	4	AGTAGCGGCATGTCATCTTC	CATGGTTCGTGCCCATTCA	20	19	50.0	52.6	57.8	58.4	1.4	0.0	1.665568	125554	126513	1140-1145	1.000000	66.7
ccsA_p5	ccsA	5	GTAGCGGCATGTCATCTTCT	CATGGTTCGTGCCCATTCA	20	19	50.0	52.6	57.8	58.4	1.4	0.0	1.665568	125554	126513	1139-1144	1.000000	66.7
ccsA-ndhD_p1	ccsA-ndhD	1	TTCGGCAATTGTAGCGTGTA	ACCCGGATTTCGTTCTTTCA	20	20	45.0	45.0	57.9	57.4	7.4	0.7	0.636096	126514	126753	426-426	1.000000	76.0
ccsA-ndhD_p2	ccsA-ndhD	2	TTCGGCAATTGTAGCGTGTA	TCGGTTGACAAGGTTGAAGT	20	20	45.0	45.0	57.9	57.3	7.4	0.0	0.784317	126514	126753	403-403	1.000000	74.9
ccsA-ndhD_p3	ccsA-ndhD	3	TCGGCAATTGTAGCGTGTAT	ACCCGGATTTCGTTCTTTCA	20	20	45.0	45.0	57.7	57.4	7.4	0.7	0.857533	126514	126753	425-425	1.000000	74.3
ccsA-ndhD_p4	ccsA-ndhD	4	TTCGGCAATTGTAGCGTGTA	CCCGGATTTCGTTCTTTCAC	20	20	45.0	50.0	57.9	57.1	7.4	0.7	0.976032	126514	126753	425-425	1.000000	73.3
ccsA-ndhD_p5	ccsA-ndhD	5	TTCGGCAATTGTAGCGTGTA	TGGTATGTACCCGGATTTCG	20	20	45.0	50.0	57.9	57.1	7.4	0.7	0.976489	126514	126753	434-434	1.000000	73.3
ycf1_p1	ycf1	1	AGTCCAATAGCGGGTCTTTC	GGAATCGGAATTGGAATCGGA	20	21	50.0	47.6	57.3	58.2	0.0	0.0	1.847074	139140	139259	308-308	1.000000	69.7
ycf1_p2	ycf1	2	GTCCAATAGCGGGTCTTTCT	GGAATCGGAATTGGAATCGGA	20	21	50.0	47.6	57.3	58.2	0.0	0.0	1.847074	139140	139259	307-307	1.000000	69.7
ycf1_p3	ycf1	3	AGTCCAATAGCGGGTCTTTC	GGAATCGGAATTGGAATCGG	20	20	50.0	50.0	57.3	56.6	0.0	0.0	2.044454	139140	139259	308-308	1.000000	68.2
ycf1_p4	ycf1	4	GTCCAATAGCGGGTCTTTCT	GGAATCGGAATTGGAATCGG	20	20	50.0	50.0	57.3	56.6	0.0	0.0	2.044454	139140	139259	307-307	1.000000	68.2
ycf1_p5	ycf1	5	GTCCAATAGCGGGTCTTTCT	CGGAATTGGAATCGGAATTGG	20	21	50.0	47.6	57.3	57.6	0.0	0.0	2.047356	139140	139259	302-302	1.000000	68.2