primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	rbcL-atpB	1	AACACCAGCTTTGAACCCAA	AGCTACATCTAGGACCGGAC	20	20	45.0	55.0	58.1	58.0	0.0	9.5	0.156632	6885	7632	900-903	1.000000	82.8
rbcL-atpB_p2	rbcL-atpB	2	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.199291	6885	7632	911-914	1.000000	82.5
rbcL-atpB_p3	rbcL-atpB	3	AACACCAGCTTTGAACCCAA	CGGACCGATTATTTGGACGA	20	20	45.0	50.0	58.1	57.8	0.0	8.6	0.353567	6885	7632	885-888	1.000000	81.3
rbcL-atpB_p4	rbcL-atpB	4	GCTTTGAACCCAACACTTGC	AGCTACATCTAGGACCGGAC	20	20	50.0	55.0	58.4	58.0	0.0	9.5	0.445121	6885	7632	893-896	1.000000	80.5
rbcL-atpB_p5	rbcL-atpB	5	AACACCAGCTTTGAACCCAA	ACCGGACCGATTATTTGGAC	20	20	45.0	50.0	58.1	57.7	0.0	17.5	0.461443	6885	7632	887-890	1.000000	80.4
atpB_p1	atpB	1	AACCGATCAACTTGCTTTGT	ACAATTCGATTGGGGGTCAG	20	20	40.0	50.0	56.2	57.9	0.0	10.8	1.962299	7633	9129	1611-1611	1.000000	68.4
atpB_p2	atpB	2	AACCGATCAACTTGCTTTGT	CAATTCGATTGGGGGTCAGT	20	20	40.0	50.0	56.2	57.9	0.0	0.0	1.962299	7633	9129	1610-1610	1.000000	68.4
atpB_p3	atpB	3	ACCGATCAACTTGCTTTGTT	ACAATTCGATTGGGGGTCAG	20	20	40.0	50.0	56.2	57.9	0.0	10.8	1.962299	7633	9129	1610-1610	1.000000	68.4
atpB_p4	atpB	4	ACCGATCAACTTGCTTTGTT	CAATTCGATTGGGGGTCAGT	20	20	40.0	50.0	56.2	57.9	0.0	0.0	1.962299	7633	9129	1609-1609	1.000000	68.4
atpB_p5	atpB	5	ACCGATCAACTTGCTTTGTT	TGGGGGTCAGTACACAAAGA	20	20	40.0	50.0	56.2	58.2	0.0	0.0	2.024395	7633	9129	1600-1600	1.000000	67.9
ndhK_p1	ndhK	1	ATGGCGAAAGGGAGCATTAG	TTTGTACAAAAGGATCCTGT	20	20	50.0	35.0	58.0	52.3	0.0	17.0	5.763658	11403	12086	777-777	1.000000	44.1
ndhK_p2	ndhK	2	GGCGAAAGGGAGCATTAGAA	TTTGTACAAAAGGATCCTGT	20	20	50.0	35.0	58.0	52.3	0.0	17.0	5.774917	11403	12086	775-775	1.000000	44.1
ndhK_p3	ndhK	3	ATGGCGAAAGGGAGCATTAG	TTTTGTACAAAAGGATCCTGT	20	21	50.0	33.3	58.0	53.2	0.0	26.0	5.802314	11403	12086	778-778	1.000000	44.1
ndhK_p4	ndhK	4	GGCGAAAGGGAGCATTAGAA	TTTTGTACAAAAGGATCCTGT	20	21	50.0	33.3	58.0	53.2	0.0	26.0	5.813573	11403	12086	776-776	1.000000	44.1
ndhK_p5	ndhK	5	TTTATGCATGGCGAAAGGGA	TTTGTACAAAAGGATCCTGT	20	20	45.0	35.0	57.9	52.3	10.2	17.0	5.872829	11403	12086	784-784	1.000000	44.1
ndhJ-trnF-GAA_p1	ndhJ-trnF-GAA	1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	20	20	50.0	55.0	57.9	58.0	3.3	10.3	0.074283	12713	13451	879-879	1.000000	83.6
ndhJ-trnF-GAA_p2	ndhJ-trnF-GAA	2	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	20	20	50.0	50.0	57.9	58.1	3.3	0.0	0.123673	12713	13451	843-843	1.000000	83.2
ndhJ-trnF-GAA_p3	ndhJ-trnF-GAA	3	TTGGATAGGATGGCCTTTGC	AGAATGGTCGGGATAGCTCA	20	20	50.0	50.0	57.9	57.9	3.3	0.0	0.159882	12713	13451	888-888	1.000000	82.9
ndhJ-trnF-GAA_p4	ndhJ-trnF-GAA	4	TTGGATAGGATGGCCTTTGC	CTGGTAGAGCAGAGGACTGA	20	20	50.0	55.0	57.9	57.9	3.3	2.5	0.208076	12713	13451	867-867	1.000000	82.5
ndhJ-trnF-GAA_p5	ndhJ-trnF-GAA	5	TTGGATAGGATGGCCTTTGC	AGTTCAAATCTGGTTCCCGG	20	20	50.0	50.0	57.9	57.8	3.3	0.0	0.272150	12713	13451	831-831	1.000000	82.0
ycf3-psaA_p1	ycf3-psaA	1	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	20	20	50.0	50.0	58.0	57.9	6.2	4.1	0.117992	19715	20677	1109-1111	1.000000	83.2
ycf3-psaA_p2	ycf3-psaA	2	AGCCCTTACCCCTGGTAATT	GCCCATTCCTCGAAAGAAGT	20	20	50.0	50.0	58.0	57.9	6.2	4.1	0.119474	19715	20677	1108-1110	1.000000	83.2
ycf3-psaA_p3	ycf3-psaA	3	AGCTATAGCCCTTACCCCTG	TGCCCATTCCTCGAAAGAAG	20	20	55.0	50.0	58.0	57.9	21.2	4.1	0.145355	19715	20677	1115-1117	1.000000	83.0
ycf3-psaA_p4	ycf3-psaA	4	AGCTATAGCCCTTACCCCTG	GCCCATTCCTCGAAAGAAGT	20	20	55.0	50.0	58.0	57.9	21.2	4.1	0.146838	19715	20677	1114-1116	1.000000	83.0
ycf3-psaA_p5	ycf3-psaA	5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	20	20	50.0	50.0	58.1	57.9	0.0	4.1	0.204161	19715	20677	1073-1075	1.000000	82.5
trnG-UCC-psbZ_p1	trnG-UCC-psbZ	1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	20	20	50.0	55.0	57.8	58.0	1.1	0.0	0.185568	26061	26872	948-1008	1.000000	82.6
trnG-UCC-psbZ_p2	trnG-UCC-psbZ	2	TACATAAAAAGGCAGGGGCG	CTTCTCCTGATGGTTGGTCG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.258054	26061	26872	988-1048	1.000000	82.0
trnG-UCC-psbZ_p3	trnG-UCC-psbZ	3	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	20	20	50.0	50.0	57.8	57.8	1.1	0.0	0.374855	26061	26872	951-1011	1.000000	81.1
trnG-UCC-psbZ_p4	trnG-UCC-psbZ	4	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	20	20	50.0	50.0	57.8	57.8	1.1	0.0	0.395214	26061	26872	944-1004	1.000000	80.9
trnG-UCC-psbZ_p5	trnG-UCC-psbZ	5	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	20	20	50.0	55.0	57.8	58.3	1.1	0.0	0.425837	26061	26872	949-1009	1.000000	80.7
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.165150	31531	32339	949-950	1.000000	82.8
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GGCGTTACTCTACCACACTG	TTTCGTAGTACCCTACCCCC	20	20	55.0	55.0	58.0	57.8	0.0	0.0	0.167010	31531	32339	924-925	1.000000	82.8
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.175803	31531	32339	912-913	1.000000	82.7
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	GGCGTTACTCTACCACACTG	CGTTGCCTCCTTGAAAGAGA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.177663	31531	32339	887-888	1.000000	82.7
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.223247	31531	32339	928-929	1.000000	82.3
petN-trnC-GCA_p1	petN-trnC-GCA	1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.088172	34149	35277	1204-1207	1.000000	83.5
petN-trnC-GCA_p2	petN-trnC-GCA	2	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.273256	34149	35277	1198-1201	1.000000	82.0
petN-trnC-GCA_p3	petN-trnC-GCA	3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.0	58.4	0.0	14.7	0.361535	34149	35277	1222-1225	1.000000	81.3
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGAGTCCACTTCTTCCCCAT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.432990	34149	35277	1258-1261	1.000000	80.7
petN-trnC-GCA_p5	petN-trnC-GCA	5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	20	20	50.0	50.0	57.8	58.4	0.0	14.7	0.546619	34149	35277	1216-1219	1.000000	79.8
atpI-atpH_p1	atpI-atpH	1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.3	58.0	11.2	0.0	0.773474	48873	50306	1595-1596	1.000000	78.0
atpI-atpH_p2	atpI-atpH	2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.800002	48873	50306	1631-1632	1.000000	77.7
atpI-atpH_p3	atpI-atpH	3	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	20	20	50.0	50.0	57.2	58.0	20.7	0.0	0.836783	48873	50306	1580-1581	1.000000	77.5
atpI-atpH_p4	atpI-atpH	4	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	20	20	50.0	45.0	57.3	58.1	11.2	1.4	0.847416	48873	50306	1505-1506	1.000000	77.4
atpI-atpH_p5	atpI-atpH	5	ACAAGCGGTATTCAAGCTCT	AACAGAAGCGGCAGAAATCA	20	20	45.0	45.0	57.2	58.1	0.0	1.4	0.873944	48873	50306	1541-1542	1.000000	77.2
atpH-atpF_p1	atpH-atpF	1	CCGGAAGCAGAGGGAAAAAT	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	58.2	57.9	0.0	13.0	0.274437	50553	51204	819-936	1.000000	82.3
atpH-atpF_p2	atpH-atpF	2	CCGGAAGCAGAGGGAAAAAT	CGGATGGCCAGTAACTCAAG	20	20	50.0	55.0	58.2	58.3	0.0	13.0	0.503924	50553	51204	821-938	1.000000	80.4
atpH-atpF_p3	atpH-atpF	3	CCGGAAGCAGAGGGAAAAAT	TGGCCAGTAACTCAAGCAAA	20	20	50.0	45.0	58.2	57.6	0.0	0.0	0.517241	50553	51204	817-934	1.000000	80.3
atpH-atpF_p4	atpH-atpF	4	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	20	20	50.0	50.0	57.6	57.9	0.0	13.0	0.532228	50553	51204	748-865	1.000000	80.2
atpH-atpF_p5	atpH-atpF	5	CCGGAAGCAGAGGGAAAAAT	ATCAAACCCGAAACTTCCGG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.630253	50553	51204	841-958	1.000000	79.4
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	TGAATCAAGCAAGGGATCCC	CAATCCGACGCTTTAGTCCA	20	20	50.0	50.0	57.6	58.0	24.6	0.0	0.446819	55409	56305	1001-1013	1.000000	81.9
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	TGAATCAAGCAAGGGATCCC	AATCCGACGCTTTAGTCCAC	20	20	50.0	50.0	57.6	58.0	24.6	0.0	0.448207	55409	56305	1000-1012	1.000000	81.9
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	TGAATCAAGCAAGGGATCCC	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.6	58.2	24.6	0.0	0.593585	55409	56305	1054-1066	1.000000	80.7
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	TGAATCAAGCAAGGGATCCC	AGCAATCCGACGCTTTAGTC	20	20	50.0	50.0	57.6	58.4	24.6	0.0	0.800659	55409	56305	1003-1015	1.000000	79.1
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	TGAATCAAGCAAGGGATCCC	CGACGCTTTAGTCCACTCAG	20	20	50.0	55.0	57.6	58.4	24.6	0.0	0.823376	55409	56305	996-1008	1.000000	78.9
ycf4_p1	ycf4	1	ATTTCCGGCAATTGCAATGG	TTGTAAACGTGTTTCCGGGT	20	20	45.0	45.0	58.0	58.0	30.3	0.0	0.062533	63114	63752	1439-1439	1.000000	83.7
ycf4_p2	ycf4	2	GAGGGCGAAATTCATCCAGT	TTGTAAACGTGTTTCCGGGT	20	20	50.0	45.0	58.0	58.0	4.8	0.0	0.079653	63114	63752	1895-1900	1.000000	83.5
ycf4_p3	ycf4	3	ATTTCCGGCAATTGCAATGG	CGTGTTTCCGGGTATTCCTT	20	20	45.0	50.0	58.0	57.9	30.3	0.0	0.130540	63114	63752	1432-1432	1.000000	83.1
ycf4_p4	ycf4	4	GGCAATTGCAATGGCTTCTT	TTGTAAACGTGTTTCCGGGT	20	20	45.0	45.0	57.9	58.0	32.4	0.0	0.139067	63114	63752	1433-1433	1.000000	83.1
ycf4_p5	ycf4	5	GAGGGCGAAATTCATCCAGT	CGTGTTTCCGGGTATTCCTT	20	20	50.0	50.0	58.0	57.9	4.8	0.0	0.147660	63114	63752	1888-1893	1.000000	83.0
petL-petG_p1	petL-petG	1	AGTCTGAATAAGATACGACTTATTTGA	GCAGTTACAAATAATCCAGCCA	27	22	29.6	40.9	56.5	57.0	19.0	0.0	11.512631	69199	69361	264-264	1.000000	40.6
petL-petG_p2	petL-petG	2	AGTCTGAATAAGATACGACTTATTTGA	TGCAGTTACAAATAATCCAGCC	27	22	29.6	40.9	56.5	57.0	19.0	0.0	11.512631	69199	69361	265-265	1.000000	40.6
petL-petG_p3	petL-petG	3	AGTCTGAATAAGATACGACTTAT	GCAGTTACAAATAATCCAGCCA	23	22	30.4	40.9	52.3	57.0	0.0	0.0	11.685353	69199	69361	264-264	1.000000	40.6
petL-petG_p4	petL-petG	4	AGTCTGAATAAGATACGACTTAT	TGCAGTTACAAATAATCCAGCC	23	22	30.4	40.9	52.3	57.0	0.0	0.0	11.685353	69199	69361	265-265	1.000000	40.6
petL-petG_p5	petL-petG	5	GTCTGAATAAGATACGACTTATTTGA	GCAGTTACAAATAATCCAGCCA	26	22	30.8	40.9	55.3	57.0	19.0	0.0	11.731381	69199	69361	263-263	1.000000	40.6
clpP-psbB_p1	clpP-psbB	1	GATGCTTCATCGTCTCCAGG	TGGACAGAAAGTAAACGGCC	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.249846	74724	75598	999-999	1.000000	78.3
clpP-psbB_p2	clpP-psbB	2	GATGCTTCATCGTCTCCAGG	AAACGGCCGGGATCATTTAA	20	20	55.0	45.0	58.1	57.9	0.0	31.8	0.268509	74724	75598	987-987	1.000000	78.2
clpP-psbB_p3	clpP-psbB	3	ATCGTCTCCAGGAACTCGAA	TGGACAGAAAGTAAACGGCC	20	20	50.0	50.0	58.2	58.1	12.3	0.0	0.277619	74724	75598	991-991	1.000000	78.1
clpP-psbB_p4	clpP-psbB	4	ATCGTCTCCAGGAACTCGAA	AAACGGCCGGGATCATTTAA	20	20	50.0	45.0	58.2	57.9	12.3	31.8	0.296282	74724	75598	979-979	1.000000	78.0
clpP-psbB_p5	clpP-psbB	5	GATGCTTCATCGTCTCCAGG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.374025	74724	75598	951-951	1.000000	77.3
ndhB-rps7_p1	ndhB-rps7	1	CTCCATCGGAACAATAGGGC	ATGGCAGAGGCAAATAGAGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.137310	98972	99338	591-591	1.000000	79.6
ndhB-rps7_p2	ndhB-rps7	2	CTCCATCGGAACAATAGGGC	CTGTGGAAAGCCGTATTCGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.120347	98972	99338	1177-1177	1.000000	79.4
ndhB-rps7_p3	ndhB-rps7	3	CTCCATCGGAACAATAGGGC	TCTGTGGAAAGCCGTATTCG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.120347	98972	99338	1178-1178	1.000000	79.4
ndhB-rps7_p4	ndhB-rps7	4	CTCCATCGGAACAATAGGGC	ACGGATTCTCCCTTTTACGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.135712	98972	99338	1042-1042	1.000000	79.4
ndhB-rps7_p5	ndhB-rps7	5	TCGGGAGTCCATTGATGAGA	CTGTGGAAAGCCGTATTCGA	20	20	50.0	50.0	57.8	58.0	12.3	0.0	0.163762	98972	99338	1702-1702	1.000000	79.0
ycf1_p1	ycf1	1	CAAGAACTGGTCATTCCCCC	AACAAATGCCATCCCAGGTT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.189138	113277	113396	318-318	1.000000	79.5
ycf1_p2	ycf1	2	CAAGAACTGGTCATTCCCCC	ACAAATGCCATCCCAGGTTT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.189138	113277	113396	317-317	1.000000	79.5
ycf1_p3	ycf1	3	CCAAGAACTGGTCATTCCCC	AACAAATGCCATCCCAGGTT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.189138	113277	113396	319-319	1.000000	79.5
ycf1_p4	ycf1	4	CCAAGAACTGGTCATTCCCC	ACAAATGCCATCCCAGGTTT	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.189138	113277	113396	318-318	1.000000	79.5
ycf1_p5	ycf1	5	CAAGAACTGGTCATTCCCCC	AAATGCCATCCCAGGTTTCT	20	20	55.0	45.0	58.2	57.4	0.0	0.0	0.789594	113277	113396	315-315	1.000000	74.7
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TCCTAAGAGCAGCGTGTCTA	TGGACAGCATTGAAAGCCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	126421	127073	773-793	1.000000	79.4
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TCCTAAGAGCAGCGTGTCTA	TTGGACAGCATTGAAAGCCT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.124629	126421	127073	774-794	1.000000	79.4
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCAGCGTGTCTACCAATTT	TGGACAGCATTGAAAGCCTT	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.185356	126421	127073	766-786	1.000000	78.9
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGCAGCGTGTCTACCAATTT	TTGGACAGCATTGAAAGCCT	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.185356	126421	127073	767-787	1.000000	78.9
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CTAAGAGCAGCGTGTCTACC	TGGACAGCATTGAAAGCCTT	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.196647	126421	127073	771-791	1.000000	78.8