primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8273	9352	1154-1193	1.000000	52.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8273	9352	1157-1196	1.000000	51.3
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8273	9352	1156-1195	1.000000	51.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.773135	8273	9352	1210-1249	1.000000	50.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8273	9352	1155-1194	1.000000	50.4
trnG-UCC_p1	trnG-UCC	1	TGATTGCGCCTCACTCTTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	9353	10109	876-876	1.000000	49.1
trnG-UCC_p2	trnG-UCC	2	TTGATTGCGCCTCACTCTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	9353	10109	877-877	1.000000	49.1
trnG-UCC_p3	trnG-UCC	3	GATTGCGCCTCACTCTTTTG	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	57.7	57.2	4.2	18.0	4.046422	9353	10109	875-875	1.000000	47.9
trnG-UCC_p4	trnG-UCC	4	ATGCTATCTTGATTGCGCCT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	57.7	57.2	4.2	18.0	4.060204	9353	10109	885-885	1.000000	47.8
trnG-UCC_p5	trnG-UCC	5	ATCTTGATTGCGCCTCACTC	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	58.3	57.2	4.2	18.0	4.131689	9353	10109	880-880	1.000000	47.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.0	58.6	1.4	16.5	0.624009	14164	14980	904-906	1.000000	75.5
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	14164	14980	876-878	1.000000	75.5
atpH-atpI_p3	atpH-atpI	3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	58.0	58.6	3.3	16.5	0.633001	14164	14980	987-989	1.000000	75.4
atpH-atpI_p4	atpH-atpI	4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.0	57.4	3.3	21.3	0.634438	14164	14980	959-961	1.000000	75.4
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	57.9	58.6	0.0	16.5	0.697901	14164	14980	960-962	1.000000	74.9
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	28672	29069	537-554	1.000000	71.5
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	28672	29069	532-549	1.000000	70.6
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	28672	29069	482-499	1.000000	70.4
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	28672	29069	541-558	1.000000	68.5
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	28672	29069	540-557	1.000000	67.8
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	57.9	58.0	3.8	0.0	0.112957	29713	30054	521-521	1.000000	79.9
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.130177	29713	30054	464-464	1.000000	79.8
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	57.9	58.4	3.8	0.0	0.420330	29713	30054	488-488	1.000000	77.5
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.425635	29713	30054	524-524	1.000000	77.4
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CATCGGATCCTAGTTCGGGA	AATTCTTCCTGGGTCGATGC	20	20	55.0	50.0	58.4	58.0	10.8	0.0	0.426800	29713	30054	525-525	1.000000	77.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	8.5	0.0	0.269796	49279	50691	1557-1565	1.000000	78.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	8.5	0.0	0.280450	49279	50691	1549-1557	1.000000	78.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	58.1	58.5	8.5	0.0	0.546572	49279	50691	1639-1647	1.000000	75.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	8.5	0.0	0.557226	49279	50691	1631-1639	1.000000	75.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	8.5	0.0	0.613568	49279	50691	1556-1564	1.000000	75.3
atpB-rbcL_p1	atpB-rbcL	1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.213422	53669	54439	909-909	1.000000	78.5
atpB-rbcL_p2	atpB-rbcL	2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	57.9	58.1	2.1	0.0	0.255358	53669	54439	887-887	1.000000	78.1
atpB-rbcL_p3	atpB-rbcL	3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.514303	53669	54439	910-910	1.000000	76.1
atpB-rbcL_p4	atpB-rbcL	4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	57.9	58.4	2.1	0.0	0.556239	53669	54439	888-888	1.000000	75.7
atpB-rbcL_p5	atpB-rbcL	5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.5	58.1	8.0	0.0	0.616166	53669	54439	888-888	1.000000	75.3
rbcL-accD_p1	rbcL-accD	1	GCAAATGGAGCCCTGAACTA	GGGTTCTTCACTTCCACTGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.231211	55904	56695	1005-1010	1.000000	78.8
rbcL-accD_p2	rbcL-accD	2	TAGCAAATGGAGCCCTGAAC	GGGTTCTTCACTTCCACTGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.231211	55904	56695	1007-1012	1.000000	78.8
rbcL-accD_p3	rbcL-accD	3	TGAAGCTAGCAAATGGAGCC	GGGTTCTTCACTTCCACTGG	20	20	50.0	55.0	58.2	58.1	18.8	0.0	0.347261	55904	56695	1013-1018	1.000000	77.9
rbcL-accD_p4	rbcL-accD	4	CCGTGAAGCTAGCAAATGGA	GGGTTCTTCACTTCCACTGG	20	20	50.0	55.0	58.3	58.1	18.8	0.0	0.364602	55904	56695	1016-1021	1.000000	77.7
rbcL-accD_p5	rbcL-accD	5	TCCGTGAAGCTAGCAAATGG	GGGTTCTTCACTTCCACTGG	20	20	50.0	55.0	58.3	58.1	18.8	0.0	0.364602	55904	56695	1017-1022	1.000000	77.7
accD-psaI_p1	accD-psaI	1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	58172	59105	1044-1075	1.000000	80.5
accD-psaI_p2	accD-psaI	2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	58172	59105	1045-1076	1.000000	80.5
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	58172	59105	1050-1081	1.000000	79.9
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	58172	59105	1051-1082	1.000000	79.9
accD-psaI_p5	accD-psaI	5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	58172	59105	1050-1081	1.000000	79.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.350824	62217	63151	1086-1087	1.000000	78.3
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.466931	62217	63151	1082-1083	1.000000	77.4
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.467291	62217	63151	1069-1070	1.000000	77.4
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.468635	62217	63151	1085-1086	1.000000	77.4
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	62217	63151	1083-1084	1.000000	77.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.386119	66049	66426	533-534	1.000000	77.5
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.387463	66049	66426	532-533	1.000000	77.5
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACCCGTGACATTTTGTACCC	ACCTGCTAAAGACCCAAACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.387732	66049	66426	534-535	1.000000	77.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CCCGTGACATTTTGTACCCA	ACCTGCTAAAGACCCAAACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.389076	66049	66426	533-534	1.000000	77.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ACAGGATTTGAACCCGTGAC	CCTGCTAAAGACCCAAACCA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.394155	66049	66426	544-545	1.000000	77.4
rpl16_p1	rpl16	1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	20	26	40.0	30.8	54.9	58.1	0.0	0.0	9.192583	80917	82467	1662-1698	1.000000	42.0
rpl16_p2	rpl16	2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	20	25	40.0	32.0	54.9	56.9	0.0	0.0	9.198462	80917	82467	1661-1697	1.000000	42.0
rpl16_p3	rpl16	3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	80917	82467	1662-1698	1.000000	42.0
rpl16_p4	rpl16	4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	80917	82467	1663-1699	1.000000	42.0
rpl16_p5	rpl16	5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	21	25	38.1	32.0	55.7	56.9	0.0	0.0	9.366175	80917	82467	1661-1697	1.000000	42.0
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	98786	100332	1661-1704	1.000000	79.0
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	98786	100332	1600-1643	1.000000	78.8
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	98786	100332	1599-1642	1.000000	78.8
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	98786	100332	1604-1647	1.000000	78.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	98786	100332	1602-1645	1.000000	77.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.630573	113116	113697	675-703	1.000000	59.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.631944	113116	113697	676-704	1.000000	59.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	55.4	57.9	9.5	0.0	2.747020	113116	113697	685-713	1.000000	58.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	113116	113697	684-712	1.000000	58.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	113116	113697	683-711	1.000000	58.0
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	116909	117542	828-839	1.000000	78.3
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	116909	117542	827-838	1.000000	78.3
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	116909	117542	848-859	1.000000	78.2
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	116909	117542	826-837	1.000000	77.2
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	116909	117542	803-814	1.000000	76.7
ycf1_p1	ycf1	1	GGCACAAATTCTGGGAGGAA	TGCTACACATAAAAATCTGGA	20	21	50.0	33.3	58.1	52.8	0.0	0.0	6.238237	124794	124913	240-309	1.000000	43.5
ycf1_p2	ycf1	2	AGTGGCACAAATTCTGGGAG	TGCTACACATAAAAATCTGGA	20	21	50.0	33.3	58.1	52.8	21.4	0.0	6.246452	124794	124913	243-312	1.000000	43.5
ycf1_p3	ycf1	3	AGAGTGGCACAAATTCTGGG	TGCTACACATAAAAATCTGGA	20	21	50.0	33.3	58.1	52.8	21.4	0.0	6.246452	124794	124913	245-314	1.000000	43.4
ycf1_p4	ycf1	4	GAGTGGCACAAATTCTGGGA	TGCTACACATAAAAATCTGGA	20	21	50.0	33.3	58.1	52.8	21.4	0.0	6.251893	124794	124913	244-313	1.000000	43.4
ycf1_p5	ycf1	5	ATGCATCAAAGAGTGGCACA	TGCTACACATAAAAATCTGGA	20	21	45.0	33.3	58.1	52.8	21.4	0.0	6.247815	124794	124913	254-323	1.000000	43.3
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	137952	139500	1661-1706	1.000000	79.1
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	137952	139500	1600-1645	1.000000	78.8
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	137952	139500	1604-1649	1.000000	78.8
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	137952	139500	1599-1644	1.000000	78.8
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	137952	139500	1602-1647	1.000000	77.9