primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	atpA	1	GAGCTGATCTCCACGTTTGT	AGAAAATCGTTACGATGGACT	20	21	50.0	38.1	57.9	55.1	0.0	21.2	4.014993	26092	28599	2865-2929	1.000000	59.5
atpA_p2	atpA	2	GAGCTGATCTCCACGTTTGT	TCGTTACGATGGACTATTTCA	20	21	50.0	38.1	57.9	54.5	0.0	2.3	4.553092	26092	28599	2859-2923	1.000000	55.2
atpA_p3	atpA	3	TTAACTAACCGACGCTCTAC	AGGGAAAAAGAGGCCATTGA	20	20	45.0	45.0	54.7	57.0	0.0	5.0	4.308714	26092	28599	2991-2991	0.500000	25.5
atpA_p4	atpA	4	AATTAACTAACCGACGCTCT	AGGGAAAAAGAGGCCATTGA	20	20	40.0	45.0	54.5	57.0	0.0	5.0	4.474647	26092	28599	2993-2993	0.500000	24.2
atpA_p5	atpA	5	TGAAAATTAACTAACCGACGCTC	AGGGAAAAAGAGGCCATTGA	23	20	39.1	45.0	57.5	57.0	0.0	5.0	4.519207	26092	28599	2997-2997	0.500000	23.8
rpl12-cysT_p1	rpl12-cysT	1	TGCCCGGTGAAGATCAAAAA	GACCAAGCCGCTTCTTCTAA	20	20	45.0	50.0	58.0	57.9	0.0	1.6	0.106469	33869	34550	1370-1373	1.000000	88.1
rpl12-cysT_p2	rpl12-cysT	2	TGCCCGGTGAAGATCAAAAA	CTAATGACCAAGCCGCTTCT	20	20	45.0	50.0	58.0	58.0	0.0	1.6	0.041545	33869	34550	1378-1378	0.500000	59.7
rpl12-cysT_p3	rpl12-cysT	3	GGCGCGAAAGTCAAAATTCA	CTAATGACCAAGCCGCTTCT	20	20	45.0	50.0	58.0	58.0	12.1	1.6	0.074726	33869	34550	1205-1205	0.500000	59.4
rpl12-cysT_p4	rpl12-cysT	4	CAGGCGCGAAAGTCAAAATT	CTAATGACCAAGCCGCTTCT	20	20	45.0	50.0	57.9	58.0	12.1	1.6	0.080315	33869	34550	1207-1207	0.500000	59.4
rpl12-cysT_p5	rpl12-cysT	5	TGCCCGGTGAAGATCAAAAA	ATTCACCAATCGCACGAGAA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.105013	33869	34550	1480-1480	0.500000	59.2
rpl36-infA_p1	rpl36-infA	1	ACAGAGGCACGTATTTTCAT	TATGATTTAACACGTGGGCG	20	20	40.0	45.0	55.1	56.2	0.0	21.6	4.700330	39942	39995	121-125	1.000000	50.9
rpl36-infA_p2	rpl36-infA	2	ACAGAGGCACGTATTTTCAT	ATATGATTTAACACGTGGGCG	20	21	40.0	42.9	55.1	56.8	0.0	21.6	5.172240	39942	39995	122-126	1.000000	48.4
rpl36-infA_p3	rpl36-infA	3	TACAGAGGCACGTATTTTCA	TATGATTTAACACGTGGGCG	20	20	40.0	45.0	54.4	56.2	0.0	21.6	5.337764	39942	39995	122-126	1.000000	48.4
rpl36-infA_p4	rpl36-infA	4	ACAGAGGCACGTATTTTCAT	CATATGATTTAACACGTGGGCG	20	22	40.0	45.5	55.1	58.1	0.0	21.6	5.065691	39942	39995	123-127	1.000000	48.3
rpl36-infA_p5	rpl36-infA	5	ACAGAGGCACGTATTTTCAT	CCATATGATTTAACACGTGGGC	20	22	40.0	45.5	55.1	57.8	0.0	21.6	5.161365	39942	39995	124-128	1.000000	48.3
rpl23_p1	rpl23	1	CCCGAGTCCTCCTAGCATTA	ATGCATGTATGGCTGAGTGG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.026145	45341	45982	1460-1460	0.500000	59.8
rpl23_p2	rpl23	2	CCGTGTACCTGGTGTATGTG	ATGCATGTATGGCTGAGTGG	20	20	55.0	50.0	58.0	58.0	10.2	0.0	0.027526	45341	45982	947-947	0.500000	59.8
rpl23_p3	rpl23	3	CCCGAGTCCTCCTAGCATTA	AAAAGCGGCGGACTCATAAT	20	20	55.0	45.0	58.0	58.0	0.0	9.8	0.041588	45341	45982	1438-1438	0.500000	59.7
rpl23_p4	rpl23	4	CCGTGTACCTGGTGTATGTG	AAAAGCGGCGGACTCATAAT	20	20	55.0	45.0	58.0	58.0	10.2	9.8	0.042969	45341	45982	925-925	0.500000	59.7
rpl23_p5	rpl23	5	CAAACGTTTATGCCCACCAC	ATGCATGTATGGCTGAGTGG	20	20	50.0	50.0	57.9	58.0	14.2	0.0	0.081857	45341	45982	1088-1088	0.500000	59.3
chlB_p1	chlB	1	CGGGATAGCTCAGTGGTAGA	AAGAAGTCCGCCTAATGCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047178	49227	50744	2950-2951	1.000000	88.5
chlB_p2	chlB	2	GTGGGAGGATTCGAACCTTC	AAGAAGTCCGCCTAATGCTG	20	20	55.0	50.0	58.0	58.0	16.9	0.0	0.055427	49227	50744	2831-2834	1.000000	88.4
chlB_p3	chlB	3	TCAAAAAGCCGGGATAGCTC	AAGAAGTCCGCCTAATGCTG	20	20	50.0	50.0	58.0	58.0	2.2	0.0	0.068225	49227	50744	2959-2960	1.000000	88.4
chlB_p4	chlB	4	AAAAGAGCTGAGCTGCTTGT	AAGAAGTCCGCCTAATGCTG	20	20	45.0	50.0	58.0	58.0	13.7	0.0	0.051384	49227	50744	2306-2307	1.000000	87.5
chlB_p5	chlB	5	ACACTTCAAAAAGCCGGGAT	AAGAAGTCCGCCTAATGCTG	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.034462	49227	50744	2965-2965	0.500000	59.7
psbZ-psbD_p1	psbZ-psbD	1	AATGGGTTGGCTTCGTGATT	TCGAACCAACCTCTCTCTGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.079846	58009	59186	1845-1848	1.000000	86.8
psbZ-psbD_p2	psbZ-psbD	2	GTATGGGCGTGGATGTTCTT	TCGAACCAACCTCTCTCTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041588	58009	59186	1763-1763	0.500000	59.7
psbZ-psbD_p3	psbZ-psbD	3	GTATGGGCGTGGATGTTCTT	TAGCGGCAACTCCAAAACTT	20	20	50.0	45.0	58.0	58.0	0.0	1.4	0.062727	58009	59186	2248-2248	0.500000	59.5
psbZ-psbD_p4	psbZ-psbD	4	GTATGGGCGTGGATGTTCTT	ATCGCGTTATACGGTCGAAG	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.066674	58009	59186	2147-2147	0.500000	59.5
psbZ-psbD_p5	psbZ-psbD	5	AATGGGTTGGCTTCGTGATT	TAGCGGCAACTCCAAAACTT	20	20	45.0	45.0	58.1	58.0	0.0	1.4	0.100985	58009	59186	2333-2333	0.500000	59.2
chlL-ccsA_p1	chlL-ccsA	1	CTTCTAAAACGGGCATCGGA	AACGCAGGAATTAAGGGACC	20	20	50.0	50.0	58.0	57.9	3.9	0.0	0.142819	66104	66580	1572-1572	0.500000	58.9
chlL-ccsA_p2	chlL-ccsA	2	CAACACAATCCACACCTCCA	AACGCAGGAATTAAGGGACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.144423	66104	66580	1221-1221	0.500000	58.8
chlL-ccsA_p3	chlL-ccsA	3	GACCTCCTGCTTCAACACAA	AACGCAGGAATTAAGGGACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.156959	66104	66580	1233-1233	0.500000	58.7
chlL-ccsA_p4	chlL-ccsA	4	ACAACGTAACCTCCACAACC	AACGCAGGAATTAAGGGACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.166609	66104	66580	1267-1267	0.500000	58.7
chlL-ccsA_p5	chlL-ccsA	5	CAACGTAACCTCCACAACCA	AACGCAGGAATTAAGGGACC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.167980	66104	66580	1266-1266	0.500000	58.7
ccsA_p1	ccsA	1	CAACACAATCCACACCTCCA	ACACGTTGTTTCTGACCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.070680	66581	67594	2173-2173	0.500000	59.4
ccsA_p2	ccsA	2	CAACACAATCCACACCTCCA	CACGTTGTTTCTGACCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.072041	66581	67594	2172-2172	0.500000	59.4
ccsA_p3	ccsA	3	GACCTCCTGCTTCAACACAA	ACACGTTGTTTCTGACCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.083216	66581	67594	2185-2185	0.500000	59.3
ccsA_p4	ccsA	4	GACCTCCTGCTTCAACACAA	CACGTTGTTTCTGACCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.084577	66581	67594	2184-2184	0.500000	59.3
ccsA_p5	ccsA	5	TGGCCAGTTCGACTTTCAAA	ACACGTTGTTTCTGACCTCC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.096096	66581	67594	1601-1601	0.500000	59.2
psbT-psbH_p1	psbT-psbH	1	GATATCCCATTCCGACGAGC	GATTATGAGCCTTGCGAGCT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.126009	71267	71767	1617-1633	1.000000	86.7
psbT-psbH_p2	psbT-psbH	2	GATATCCCATTCCGACGAGC	AATGGATCACGGAGTTGCTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.032169	71267	71767	1811-1811	0.500000	59.7
psbT-psbH_p3	psbT-psbH	3	TGACGACGGCATTGTAAGAG	AATGGATCACGGAGTTGCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039039	71267	71767	1833-1833	0.500000	59.7
psbT-psbH_p4	psbT-psbH	4	GACGGCATTGTAAGAGCTGA	AATGGATCACGGAGTTGCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039983	71267	71767	1829-1829	0.500000	59.7
psbT-psbH_p5	psbT-psbH	5	GCTGTTGGTTGGTTAGGTCA	AATGGATCACGGAGTTGCTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054179	71267	71767	1940-1940	0.500000	59.6
cemA_p1	cemA	1	ACGCACCTTTTGATCCACTT	AACAAGCGACCAGTGCTAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.056950	73294	74211	1594-1861	1.000000	86.3
cemA_p2	cemA	2	ACGCACCTTTTGATCCACTT	AAGCGACCAGTGCTAAACAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.056950	73294	74211	1591-1858	1.000000	86.3
cemA_p3	cemA	3	ACGCACCTTTTGATCCACTT	AGCGACCAGTGCTAAACAAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.056950	73294	74211	1590-1857	1.000000	86.3
cemA_p4	cemA	4	ACGCACCTTTTGATCCACTT	GCACGTGCTCGAAAAATTGA	20	20	45.0	45.0	58.0	58.0	0.0	32.5	0.049780	73294	74211	2176-2176	0.500000	59.6
cemA_p5	cemA	5	TGGCAAGCTTCGGTAAGTTT	GCACGTGCTCGAAAAATTGA	20	20	45.0	45.0	58.0	58.0	0.0	32.5	0.055377	73294	74211	2105-2105	0.500000	59.6
rrn16-trnS_p1	rrn16-trnS	1	CGATCGAATGATTGGGGGTT	TTAGTGAGCGCAATGGAGTC	20	20	50.0	50.0	58.0	58.0	10.3	11.5	0.042826	93279	93398	1437-1437	0.500000	59.7
rrn16-trnS_p2	rrn16-trnS	2	CGATCGAATGATTGGGGGTT	GGGAATCCAGAAGTCAGCTG	20	20	50.0	55.0	58.0	58.0	10.3	7.6	0.068211	93279	93398	1307-1307	0.500000	59.5
rrn16-trnS_p3	rrn16-trnS	3	CTTCTGATTTCGGAGTGCCA	TTAGTGAGCGCAATGGAGTC	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.109286	93279	93398	1200-1200	0.500000	59.1
rrn16-trnS_p4	rrn16-trnS	4	TCGAATGATTGGGGGTTCAC	TTAGTGAGCGCAATGGAGTC	20	20	50.0	50.0	57.9	58.0	0.0	11.5	0.132928	93279	93398	1434-1434	0.500000	58.9
rrn16-trnS_p5	rrn16-trnS	5	CACGCTTCAGCACACAATTT	TTAGTGAGCGCAATGGAGTC	20	20	45.0	50.0	57.9	58.0	0.0	11.5	0.134156	93279	93398	1094-1094	0.500000	58.9
trnS-psaJ_p1	trnS-psaJ	1	TTTAGGCGCCTCTAACGAAC	GGATCTCTCTCTTCCACGGA	20	20	50.0	55.0	58.0	57.9	36.7	0.0	0.060479	115359	115478	536-536	0.500000	59.5
trnS-psaJ_p2	trnS-psaJ	2	TTTAGGCGCCTCTAACGAAC	AGGCTGGGATCTCTCTCTTC	20	20	50.0	55.0	58.0	57.9	36.7	0.0	0.087040	115359	115478	542-542	0.500000	59.3
trnS-psaJ_p3	trnS-psaJ	3	TAGGCGCCTCTAACGAACTA	GGATCTCTCTCTTCCACGGA	20	20	50.0	55.0	58.0	57.9	36.7	0.0	0.102736	115359	115478	534-534	0.500000	59.2
trnS-psaJ_p4	trnS-psaJ	4	GCTCCCGGATCTTGTTGATT	GGATCTCTCTCTTCCACGGA	20	20	50.0	55.0	58.0	57.9	0.7	0.0	0.104173	115359	115478	964-964	0.500000	59.2
trnS-psaJ_p5	trnS-psaJ	5	TAGGCGCCTCTAACGAACTA	AGGCTGGGATCTCTCTCTTC	20	20	50.0	55.0	58.0	57.9	36.7	0.0	0.129296	115359	115478	540-540	0.500000	59.0
trnR-trnA_p1	trnR-trnA	1	TAGACCGACGCCTTATCCAT	GCACAAAAGTTCTGCGATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039776	127160	127241	841-841	0.500000	59.7
trnR-trnA_p2	trnR-trnA	2	CCACCTAGACTACGACGGAT	GCACAAAAGTTCTGCGATCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.066344	127160	127241	965-965	0.500000	59.5
trnR-trnA_p3	trnR-trnA	3	CACCAGGCCTTCAGATCTTC	GCACAAAAGTTCTGCGATCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.066427	127160	127241	1139-1139	0.500000	59.5
trnR-trnA_p4	trnR-trnA	4	CAAGCCTTGTGAAAGAAGCG	GCACAAAAGTTCTGCGATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073001	127160	127241	1228-1228	0.500000	59.4
trnR-trnA_p5	trnR-trnA	5	GACCGACGCCTTATCCATTT	GCACAAAAGTTCTGCGATCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.081431	127160	127241	839-839	0.500000	59.3
trnR-rpoB_p1	trnR-rpoB	1	TTTCGATTGAGTGAGCTCCG	GAATGTGGCCGAAACTTTGG	20	20	50.0	50.0	58.0	57.9	12.1	0.0	0.068282	133991	134110	662-662	0.500000	59.5
trnR-rpoB_p2	trnR-rpoB	2	TTTCGATTGAGTGAGCTCCG	AACGTCTTGCTTTGCCAAAC	20	20	50.0	45.0	58.0	58.1	12.1	0.0	0.082465	133991	134110	712-712	0.500000	59.3
trnR-rpoB_p3	trnR-rpoB	3	TTTCGATTGAGTGAGCTCCG	CTTGCTTTGCCAAACACGAA	20	20	50.0	45.0	58.0	58.1	12.1	0.0	0.083790	133991	134110	707-707	0.500000	59.3
trnR-rpoB_p4	trnR-rpoB	4	GTTTGATAGGGTCACCGGTC	GAATGTGGCCGAAACTTTGG	20	20	55.0	50.0	58.0	57.9	2.6	0.0	0.084521	133991	134110	396-396	0.500000	59.3
trnR-rpoB_p5	trnR-rpoB	5	GTGGAAAGTTTCGGGGAGTT	GAATGTGGCCGAAACTTTGG	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.087517	133991	134110	559-559	0.500000	59.3