primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	214	747	550-602	1.000000	84.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	214	747	564-616	1.000000	84.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	214	747	563-615	1.000000	84.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	214	747	565-617	1.000000	84.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	214	747	553-605	1.000000	84.3
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4798	6793	1928-2141	1.000000	86.7
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4798	6793	1927-2140	1.000000	86.7
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4798	6793	1914-2127	1.000000	84.4
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	6.4	16.9	0.646638	4798	6793	1939-2152	1.000000	83.5
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4798	6793	1937-2150	1.000000	82.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060735	10210	10700	1375-1531	1.000000	88.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.089572	10210	10700	1378-1534	1.000000	88.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTTGCCAGCTTCCCTAAAGG	GCGAGTTGCTCAAACAATCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.107541	10210	10700	1476-1632	1.000000	87.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AGCCTTCCAAGCTAACGATG	CTTTCTAAACAAGCGCAGGC	20	20	50.0	50.0	58.0	58.0	0.0	11.5	0.060735	10210	10700	1838-1994	1.000000	87.2
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CCTAGCCTTCCAAGCTAACG	CTTTCTAAACAAGCGCAGGC	20	20	55.0	50.0	58.1	58.0	0.0	11.5	0.089572	10210	10700	1841-1997	1.000000	87.0
atpA-atpF_p1	atpA-atpF	1	TCATCGGCTCGAATGCTTAC	CAATGAAAGAAATAACCGATTAG	20	23	50.0	30.4	58.1	52.0	1.4	0.0	9.127272	12225	12278	100-102	1.000000	44.8
atpA-atpF_p2	atpA-atpF	2	CTCATCGGCTCGAATGCTTA	CAATGAAAGAAATAACCGATTAG	20	23	50.0	30.4	57.9	52.0	1.4	0.0	9.120279	12225	12278	101-103	1.000000	44.7
atpA-atpF_p3	atpA-atpF	3	ACTTGAAGTACGGTACCGGT	CAATGAAAGAAATAACCGATTAG	20	23	50.0	30.4	58.4	52.0	35.9	0.0	9.359392	12225	12278	181-183	1.000000	42.6
atpA-atpF_p4	atpA-atpF	4	CCGTCGCCTACTTGAAGTAC	CAATGAAAGAAATAACCGATTAG	20	23	55.0	30.4	58.1	52.0	0.0	0.0	9.074765	12225	12278	190-192	1.000000	42.5
atpA-atpF_p5	atpA-atpF	5	GTCGCCTACTTGAAGTACGG	CAATGAAAGAAATAACCGATTAG	20	23	55.0	30.4	58.1	52.0	13.2	0.0	9.074765	12225	12278	188-190	1.000000	42.5
atpF-atpH_p1	atpF-atpH	1	GCGACCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.282221	13564	14269	737-845	1.000000	86.0
atpF-atpH_p2	atpF-atpH	2	GCGACCCAAAGAAACGAAAG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	58.0	57.6	0.0	3.3	0.450782	13564	14269	739-847	1.000000	84.7
atpF-atpH_p3	atpF-atpH	3	AGCGACCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.579016	13564	14269	738-846	1.000000	83.7
atpF-atpH_p4	atpF-atpH	4	ATTAAGCCCGAAACTCCCAG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.669866	13564	14269	767-875	1.000000	82.8
atpF-atpH_p5	atpF-atpH	5	AGCGACCCAAAGAAACGAAA	TGGCCTGGTTGTAGCATTAG	20	20	45.0	50.0	58.3	57.6	0.0	3.3	0.747577	13564	14269	740-848	1.000000	82.3
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33540	34871	1201-1437	1.000000	83.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	33540	34871	1180-1416	1.000000	83.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.3	58.2	0.0	0.0	0.414292	33540	34871	1198-1434	1.000000	82.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.432442	33540	34871	1177-1413	1.000000	82.6
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.546195	33540	34871	1203-1439	1.000000	81.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.293100	34946	36945	2133-2211	1.000000	84.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCCCTTTTAACTCAGTGGT	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.298271	34946	36945	2171-2249	1.000000	84.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.3	58.0	0.0	5.0	0.315771	34946	36945	2135-2213	1.000000	84.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCCCTTTTAACTCAGTGGT	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	57.7	58.0	0.0	5.0	0.320942	34946	36945	2173-2251	1.000000	84.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	CGCTATGGTAAGGCGTAAGT	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.514213	34946	36945	2144-2222	1.000000	82.5
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.0	57.8	0.0	1.1	0.185568	40262	41432	972-1446	1.000000	85.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.395214	40262	41432	968-1442	1.000000	83.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.530110	40262	41432	971-1445	1.000000	82.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.8	57.8	0.0	1.1	0.983632	40262	41432	970-1444	1.000000	79.2
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	20	19	55.0	57.9	58.0	58.2	0.0	0.0	1.205250	40262	41432	994-1468	1.000000	77.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	51546	52024	522-537	1.000000	83.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	51546	52024	553-568	1.000000	83.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	51546	52024	530-545	1.000000	83.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	51546	52024	561-576	1.000000	82.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAAAGCCAGCTATCGGAGTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.322153	51546	52024	568-583	1.000000	82.0
ndhC-trnV_p1	ndhC-trnV	1	GCCCTTTGTTAATCGGGGAT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.143344	55569	57077	1546-1663	1.000000	84.8
ndhC-trnV_p2	ndhC-trnV	2	GCCCTTTGTTAATCGGGGAT	CAGGTCTACGGTTCGAATCC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.272672	55569	57077	1555-1672	1.000000	83.7
ndhC-trnV_p3	ndhC-trnV	3	GCCCTTTGTTAATCGGGGAT	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	57.9	58.3	0.0	22.8	0.372726	55569	57077	1547-1664	1.000000	82.9
ndhC-trnV_p4	ndhC-trnV	4	TCTCGGGCCCTTTGTTAATC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.6	57.9	22.4	0.0	0.489289	55569	57077	1552-1669	1.000000	82.0
ndhC-trnV_p5	ndhC-trnV	5	GCCCTTTGTTAATCGGGGAT	GCAGGTCTACGGTTCGAATC	20	20	50.0	55.0	57.9	58.4	0.0	0.0	0.509714	55569	57077	1556-1673	1.000000	81.8
psbB-psbT_p1	psbB-psbT	1	TCCAAAAACTTGGCGATCCA	TCCCCTACAACAGTTCGTCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017077	80942	81198	746-867	1.000000	86.4
psbB-psbT_p2	psbB-psbT	2	ACTCTTTGTCCGTCGTATGC	TCCCCTACAACAGTTCGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	80942	81198	1171-1292	1.000000	85.5
psbB-psbT_p3	psbB-psbT	3	GGGATGGAATAGCTGTTGGG	TCCCCTACAACAGTTCGTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018488	80942	81198	1232-1353	1.000000	85.3
psbB-psbT_p4	psbB-psbT	4	GGGGATGGAATAGCTGTTGG	TCCCCTACAACAGTTCGTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018488	80942	81198	1233-1354	1.000000	85.3
psbB-psbT_p5	psbB-psbT	5	GCTGGGCTAGTCGAAAATCA	TCCCCTACAACAGTTCGTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.029546	80942	81198	1362-1483	1.000000	84.8
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.0	57.9	5.8	0.0	2.093188	101258	101911	741-770	1.000000	63.8
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.0	57.9	5.8	0.0	2.094558	101258	101911	739-768	1.000000	63.8
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	20	22	50.0	45.5	58.1	57.9	0.0	0.0	2.167485	101258	101911	729-758	1.000000	63.2
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCG	20	22	50.0	45.5	58.0	58.2	5.8	0.0	2.187273	101258	101911	742-771	1.000000	63.0
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	CTCGCGTGTCTACCATTTCA	ACCAGGAATATCAATGAACCCG	20	22	50.0	45.5	58.0	58.2	5.8	0.0	2.188644	101258	101911	740-769	1.000000	63.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAAAATCAAACGGTGGAATAA	AGCGTGTCTACCGATTTCAC	21	20	28.6	50.0	52.2	58.0	0.0	0.0	6.817550	121571	123507	1620-2374	1.000000	51.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAAATCAAACGGTGGAATAA	CAGCGTGTCTACCGATTTCA	21	20	28.6	50.0	52.2	58.0	0.0	0.0	6.818921	121571	123507	1621-2375	1.000000	51.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAAATCAAACGGTGGAATAA	TCCTAAGAGCAGCGTGTCTA	21	20	28.6	50.0	52.2	57.9	0.0	0.0	6.933997	121571	123507	1630-2384	1.000000	50.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAAAATCAAACGGTGGAATAA	CTAAGAGCAGCGTGTCTACC	21	20	28.6	55.0	52.2	57.8	0.0	0.0	7.006015	121571	123507	1628-2382	1.000000	50.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAAATCAAACGGTGGAATAA	AAAGATGTTATGCCGCCACT	21	20	28.6	45.0	52.2	57.9	0.0	0.0	6.938490	121571	123507	1681-2435	1.000000	50.8
rps15-ycf1_p1	rps15-ycf1	1	TGACTCCCGAATATCCAGCT	AAAACTGCAAGCCCGTTTTG	20	20	50.0	45.0	57.9	58.3	0.0	16.3	0.437280	133008	133548	985-999	1.000000	81.5
rps15-ycf1_p2	rps15-ycf1	2	GATGAGTCGAAGAACGCCAT	AAAACTGCAAGCCCGTTTTG	20	20	50.0	45.0	58.1	58.3	0.0	16.3	0.421280	133008	133548	1205-1210	1.000000	81.1
rps15-ycf1_p3	rps15-ycf1	3	CATAGGGGTTTCCTCGCTTC	AAAACTGCAAGCCCGTTTTG	20	20	55.0	45.0	58.0	58.3	0.0	16.3	0.394248	133008	133548	1125-1126	0.833333	72.2
rps15-ycf1_p4	rps15-ycf1	4	CATAGGGGTTTCCTCGCTTC	ACTGCAAGCCCGTTTTGTAT	20	20	55.0	45.0	58.0	58.4	0.0	0.0	0.428914	133008	133548	1122-1123	0.833333	71.9
rps15-ycf1_p5	rps15-ycf1	5	CATAGGGGTTTCCTCGCTTC	AAAAACTGCAAGCCCGTTTT	20	20	55.0	40.0	58.0	57.6	0.0	13.1	0.440003	133008	133548	1126-1127	0.833333	71.8
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	CCAGGAATATCAATGAACCCGA	GTCTCGCGTGTCTACCATTT	22	20	45.5	50.0	57.9	58.0	0.0	5.8	2.093188	154125	154778	741-770	1.000000	63.8
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	CCAGGAATATCAATGAACCCGA	CTCGCGTGTCTACCATTTCA	22	20	45.5	50.0	57.9	58.0	0.0	5.8	2.094558	154125	154778	739-768	1.000000	63.8
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	CCAGGAATATCAATGAACCCGA	TACCATTTCACCACCAAGGC	22	20	45.5	50.0	57.9	58.1	0.0	0.0	2.167485	154125	154778	729-758	1.000000	63.2
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	ACCAGGAATATCAATGAACCCG	GTCTCGCGTGTCTACCATTT	22	20	45.5	50.0	58.2	58.0	0.0	5.8	2.187273	154125	154778	742-771	1.000000	63.0
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	ACCAGGAATATCAATGAACCCG	CTCGCGTGTCTACCATTTCA	22	20	45.5	50.0	58.2	58.0	0.0	5.8	2.188644	154125	154778	740-769	1.000000	63.0