primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCCAT	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.137162	84	440	423-423	1.000000	78.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCCAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.141524	84	440	437-437	1.000000	78.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCCAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.143031	84	440	436-436	1.000000	78.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCCAT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.143031	84	440	438-438	1.000000	78.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TATGTTATGGGCGAACGACG	CCTCTAGACCTAGCTGCCAT	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.165522	84	440	485-485	1.000000	78.7
psbA_p1	psbA	1	CCTCTTGAAATGGATATTGCTCC	AGAATTCGTGTGCTTGGGAG	23	20	43.5	50.0	57.3	58.2	0.0	0.0	3.888836	441	1502	1175-1175	1.000000	48.9
psbA_p2	psbA	2	CCTCTTGAAATGGATATTGCTCC	AGAGAATTCGTGTGCTTGGG	23	20	43.5	50.0	57.3	58.2	0.0	0.0	3.888836	441	1502	1177-1177	1.000000	48.9
psbA_p3	psbA	3	CCTCTTGAAATGGATATTGCTCC	GAGAATTCGTGTGCTTGGGA	23	20	43.5	50.0	57.3	58.2	0.0	0.0	3.893939	441	1502	1176-1176	1.000000	48.8
psbA_p4	psbA	4	AGGAGCAATATCAATCCTCTTGA	AGAATTCGTGTGCTTGGGAG	23	20	39.1	50.0	57.1	58.2	0.0	0.0	4.087900	441	1502	1190-1190	1.000000	47.3
psbA_p5	psbA	5	AGGAGCAATATCAATCCTCTTGA	AGAGAATTCGTGTGCTTGGG	23	20	39.1	50.0	57.1	58.2	0.0	0.0	4.087900	441	1502	1192-1192	1.000000	47.3
matK_p1	matK	1	ACTTACTCCATGAACATTTCA	TCCCCTATCCCTGTCTCAAA	21	20	33.3	50.0	52.8	57.0	0.0	0.0	7.209645	2101	3618	1671-1671	1.000000	40.0
matK_p2	matK	2	ACTTACTCCATGAACATTTCA	CCCTGTTTTGACTGTATCGC	21	20	33.3	50.0	52.8	56.8	0.0	0.0	7.431698	2101	3618	1719-1719	1.000000	40.0
matK_p3	matK	3	ACTTACTCCATGAACATTTCA	AATCCCCTATCCCTGTCTCA	21	20	33.3	50.0	52.8	56.8	0.0	0.0	7.447617	2101	3618	1673-1673	1.000000	40.0
matK_p4	matK	4	ACTTACTCCATGAACATTTCA	ATCCCCTATCCCTGTCTCAA	21	20	33.3	50.0	52.8	56.8	0.0	0.0	7.447617	2101	3618	1672-1672	1.000000	40.0
matK_p5	matK	5	ACTTACTCCATGAACATTTCA	TCCCCTATCCCTGTCTCAAAT	21	21	33.3	47.6	52.8	57.6	0.0	0.0	7.686240	2101	3618	1671-1671	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	TTCCCGAAAGATAAGTGGGT	TCGGGTTGCTAACTCAATGG	20	20	45.0	50.0	56.1	57.9	0.0	0.0	1.967151	3619	4348	847-847	1.000000	64.3
matK-trnK-UUU_p2	matK-trnK-UUU	2	TTCCCGAAAGATAAGTGGGT	CGGGTTGCTAACTCAATGGT	20	20	45.0	50.0	56.1	58.2	0.0	0.0	2.037985	3619	4348	846-846	1.000000	63.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGGAGGTCATATTGCTGAAACC	TCGGGTTGCTAACTCAATGG	22	20	45.5	50.0	58.1	57.9	0.0	0.0	2.216901	3619	4348	825-825	1.000000	62.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	GGAGGTCATATTGCTGAAACCT	TCGGGTTGCTAACTCAATGG	22	20	45.5	50.0	58.1	57.9	0.0	0.0	2.216901	3619	4348	824-824	1.000000	62.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	GGAGGTCATATTGCTGAAACCT	CGGGTTGCTAACTCAATGGT	22	20	45.5	50.0	58.1	58.2	0.0	0.0	2.287735	3619	4348	823-823	1.000000	61.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGCTGGTATGAACGAATCCA	CAAAAAGGGCGGGTGTTTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.096670	4386	5265	1032-1032	1.000000	79.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCGCTGGTATGAACGAATCC	CAAAAAGGGCGGGTGTTTTT	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.250211	4386	5265	1033-1033	1.000000	78.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGCTGGTATGAACGAATCCA	GGCGGGTGTTTTTATCGAAC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.317280	4386	5265	1025-1025	1.000000	77.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCGCTGGTATGAACGAATCC	GGCGGGTGTTTTTATCGAAC	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.470822	4386	5265	1026-1026	1.000000	76.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	CAAAAAGGGCGGGTGTTTTT	20	20	50.0	45.0	58.6	58.0	6.4	0.0	0.601796	4386	5265	984-984	1.000000	75.2
rps16_p1	rps16	1	TCCACACCCTCTTTTGTTTCT	TGGCTCCAAACCAGAGAAAT	21	20	42.9	45.0	57.3	57.0	0.0	0.0	2.640915	5266	6455	1241-1241	1.000000	58.9
rps16_p2	rps16	2	GGGTATGAATAGATAGCGGGG	TGGCTCCAAACCAGAGAAAT	21	20	52.4	45.0	57.2	57.0	0.0	0.0	2.799577	5266	6455	1307-1307	1.000000	57.6
rps16_p3	rps16	3	GGTATGAATAGATAGCGGGGG	TGGCTCCAAACCAGAGAAAT	21	20	52.4	45.0	57.2	57.0	0.0	0.0	2.799577	5266	6455	1306-1306	1.000000	57.6
rps16_p4	rps16	4	TCCACACCCTCTTTTGTTTCT	GGCTCCAAACCAGAGAAATG	21	20	42.9	50.0	57.3	56.7	0.0	0.0	2.975180	5266	6455	1240-1240	1.000000	56.2
rps16_p5	rps16	5	TCCACACCCTCTTTTGTTTCTT	TGGCTCCAAACCAGAGAAAT	22	20	40.9	45.0	58.0	57.0	0.0	0.0	3.002141	5266	6455	1241-1241	1.000000	56.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACGAGGGACCC	20	20	50.0	50.0	58.0	57.6	0.0	0.6	0.424641	8261	9171	1024-1024	1.000000	76.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACGAGGGACCC	20	20	50.0	50.0	58.0	57.6	0.0	0.6	0.426029	8261	9171	1023-1023	1.000000	76.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACGAGGGACCC	20	20	50.0	50.0	58.2	57.6	0.0	0.6	0.571407	8261	9171	1078-1078	1.000000	75.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGCAATCCGACGCTTTAGTC	ATGAATCAAACGAGGGACCC	20	20	50.0	50.0	58.4	57.6	0.0	0.6	0.778481	8261	9171	1026-1026	1.000000	73.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CGACGCTTTAGTCCACTCAG	ATGAATCAAACGAGGGACCC	20	20	55.0	50.0	58.4	57.6	0.0	0.6	0.801198	8261	9171	1019-1019	1.000000	73.6
trnG-UCC_p1	trnG-UCC	1	CCGACTGATTCTCCTGTTCG	AGATTCCAATATCCGCTGAT	20	20	55.0	40.0	58.1	54.0	0.0	0.0	4.126616	9172	9965	878-878	1.000000	47.0
trnG-UCC_p2	trnG-UCC	2	CCGACTGATTCTCCTGTTCG	AGATTCCAATATCCGCTGATAGA	20	23	55.0	39.1	58.1	56.7	0.0	4.1	4.352191	9172	9965	878-878	1.000000	45.2
trnG-UCC_p3	trnG-UCC	3	CCGACTGATTCTCCTGTTCG	AGATTCCAATATCCGCTGATAGAA	20	24	55.0	37.5	58.1	57.4	0.0	4.1	4.696980	9172	9965	878-878	1.000000	42.4
trnG-UCC_p4	trnG-UCC	4	CCGACTGATTCTCCTGTTCG	TCCAATATCCGCTGATAGAA	20	20	55.0	40.0	58.1	53.3	0.0	4.1	4.771651	9172	9965	874-874	1.000000	41.8
trnG-UCC_p5	trnG-UCC	5	CCGACTGATTCTCCTGTTCG	TTCCAATATCCGCTGATAGA	20	20	55.0	40.0	58.1	53.3	0.0	4.1	4.771651	9172	9965	875-875	1.000000	41.8
rpoC2_p1	rpoC2	1	AGAAATCCCTCGAATGGTGC	GGGAGCTAGAGGAAATGCAT	20	20	50.0	50.0	58.0	57.1	0.9	0.0	0.991250	21043	21162	280-280	1.000000	72.4
rpoC2_p2	rpoC2	2	GCCGCAATCTGTTCTACGTA	GGGAGCTAGAGGAAATGCAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	1.029855	21043	21162	262-262	1.000000	72.1
rpoC2_p3	rpoC2	3	AGAAATCCCTCGAATGGTGC	GTCTTTTTCGGGAGCTAGAGG	20	21	50.0	52.4	58.0	58.1	0.9	0.0	1.149221	21043	21162	289-289	1.000000	71.1
rpoC2_p4	rpoC2	4	GCCGCAATCTGTTCTACGTA	GTCTTTTTCGGGAGCTAGAGG	20	21	50.0	52.4	58.1	58.1	0.0	0.0	1.187826	21043	21162	271-271	1.000000	70.8
rpoC2_p5	rpoC2	5	AGAAATCCCTCGAATGGTGC	TCTTTTTCGGGAGCTAGAGGA	20	21	50.0	47.6	58.0	58.2	0.9	0.0	1.227712	21043	21162	288-288	1.000000	70.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	20	18	45.0	66.7	55.8	60.1	0.0	0.0	6.355329	31587	32504	986-986	1.000000	40.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGGAACTAGAATGAACAGTGCA	GTTCGAGCCCCGTCAGTC	22	18	40.9	66.7	57.4	60.1	0.0	0.0	6.749963	31587	32504	993-993	1.000000	40.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GCAGTAGCAATAAACGCTAGA	GTTCGAGCCCCGTCAGTC	21	18	42.9	66.7	56.2	60.1	0.0	0.0	6.956951	31587	32504	974-974	1.000000	40.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GGAACTAGAATGAACAGTGCAG	GTTCGAGCCCCGTCAGTC	22	18	45.5	66.7	57.1	60.1	0.0	0.0	7.061916	31587	32504	992-992	1.000000	40.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GGCAGTAGGAACTAGAATGAACA	GTTCGAGCCCCGTCAGTC	23	18	43.5	66.7	57.9	60.1	0.0	0.0	7.212135	31587	32504	999-999	1.000000	40.0
trnD-GUC_p1	trnD-GUC	1	AGAAAGGGGGTCCTAGTTAGG	TCAATAGATCAGTCCCGCCT	21	20	52.4	50.0	57.9	57.9	0.0	0.0	1.220312	32505	32578	277-277	1.000000	70.5
trnD-GUC_p2	trnD-GUC	2	AGAAAGGGGGTCCTAGTTAGG	CGCCTCTGTCAGTTACAACA	21	20	52.4	50.0	57.9	57.9	0.0	0.0	1.281123	32505	32578	262-262	1.000000	70.1
trnD-GUC_p3	trnD-GUC	3	AGAAAGGGGGTCCTAGTTAGG	ATAGATCAGTCCCGCCTCTG	21	20	52.4	55.0	57.9	58.4	0.0	0.0	1.505521	32505	32578	274-274	1.000000	68.2
trnD-GUC_p4	trnD-GUC	4	AGAAAGGGGGTCCTAGTTAGG	AATAGATCAGTCCCGCCTCT	21	20	52.4	50.0	57.9	57.6	0.0	0.0	1.522433	32505	32578	275-275	1.000000	68.1
trnD-GUC_p5	trnD-GUC	5	AGAAAGGGGGTCCTAGTTAGG	CAATAGATCAGTCCCGCCTC	21	20	52.4	55.0	57.9	57.6	0.0	0.0	1.579452	32505	32578	276-276	1.000000	67.7
petL-petG_p1	petL-petG	1	TCTTGCCTCTTTTCATGCCT	GTTCCAACTGATCACCACGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.713149	68210	68375	385-385	1.000000	74.3
petL-petG_p2	petL-petG	2	TCTTGCCTCTTTTCATGCCT	TTCCAACTGATCACCACGTC	20	20	45.0	50.0	57.4	57.8	0.0	0.0	0.755920	68210	68375	384-384	1.000000	74.0
petL-petG_p3	petL-petG	3	TCTTGCCTCTTTTCATGCCT	AGTTCCAACTGATCACCACG	20	20	45.0	50.0	57.4	57.8	0.0	0.0	0.761935	68210	68375	386-386	1.000000	73.9
petL-petG_p4	petL-petG	4	ATCTTGCCTCTTTTCATGCCT	GTTCCAACTGATCACCACGT	21	20	42.9	50.0	57.9	58.1	0.0	0.0	1.218389	68210	68375	386-386	1.000000	70.3
petL-petG_p5	petL-petG	5	CGGCTCTATTTATTGGGCTGA	GTTCCAACTGATCACCACGT	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.247048	68210	68375	316-316	1.000000	70.0
trnW-CCA_p1	trnW-CCA	1	GAATCCACAGGAGGTCCAAA	CCAGATGTATTGGTGGGGTC	20	20	50.0	55.0	57.1	57.7	0.0	0.0	1.227514	68613	68686	178-178	1.000000	70.6
trnW-CCA_p2	trnW-CCA	2	GAATCCACAGGAGGTCCAAA	ATGCCAGATGTATTGGTGGG	20	20	50.0	50.0	57.1	57.6	0.0	0.0	1.249086	68613	68686	181-181	1.000000	70.4
trnW-CCA_p3	trnW-CCA	3	GAATCCACAGGAGGTCCAAA	TGGTGGGGTCACAAAAATGA	20	20	50.0	45.0	57.1	57.5	0.0	0.0	1.340403	68613	68686	168-168	1.000000	69.8
trnW-CCA_p4	trnW-CCA	4	ATTCGAATCCACAGGAGGTC	CCAGATGTATTGGTGGGGTC	20	20	50.0	55.0	57.0	57.7	4.4	0.0	1.338477	68613	68686	182-182	1.000000	69.7
trnW-CCA_p5	trnW-CCA	5	ATTCGAATCCACAGGAGGTC	ATGCCAGATGTATTGGTGGG	20	20	50.0	50.0	57.0	57.6	4.4	0.0	1.360049	68613	68686	185-185	1.000000	69.6
petB_p1	petB	1	GTAGTCTGACCGCGGAATTT	CTCCCCCAAACGAGAAATGA	20	20	50.0	50.0	58.0	57.5	35.9	0.0	0.495909	77260	78731	1565-1565	1.000000	76.0
petB_p2	petB	2	GTAGTCTGACCGCGGAATTT	CCTCCCCCAAACGAGAAATG	20	20	50.0	55.0	58.0	58.5	35.9	0.0	0.542296	77260	78731	1566-1566	1.000000	75.7
petB_p3	petB	3	GTAGTCTGACCGCGGAATTT	TCCTCCCCCAAACGAGAAAT	20	20	50.0	50.0	58.0	58.6	35.9	0.0	0.637597	77260	78731	1567-1567	1.000000	74.9
petB_p4	petB	4	TCTGACCGCGGAATTTCTTT	CTCCCCCAAACGAGAAATGA	20	20	45.0	50.0	57.8	57.5	35.9	0.0	0.668850	77260	78731	1561-1561	1.000000	74.6
petB_p5	petB	5	GTAGTCTGACCGCGGAATTT	ACTATCCTTCCTCCCCCAAA	20	20	50.0	50.0	58.0	57.3	35.9	0.0	0.686409	77260	78731	1575-1575	1.000000	74.5
petD_p1	petD	1	TGTTTCCCCTCAACTTCACA	CGTTTCAAAGCGAATTCTCCC	20	21	45.0	47.6	56.9	58.1	0.0	3.5	2.261121	78936	80172	1400-1400	1.000000	62.0
petD_p2	petD	2	GGATGTTTCCCCTCAACTTCA	CGTTTCAAAGCGAATTCTCCC	21	21	47.6	47.6	57.8	58.1	0.0	3.5	2.303293	78936	80172	1403-1403	1.000000	61.6
petD_p3	petD	3	TGGATGTTTCCCCTCAACTTC	CGTTTCAAAGCGAATTCTCCC	21	21	47.6	47.6	57.8	58.1	0.0	3.5	2.303293	78936	80172	1404-1404	1.000000	61.6
petD_p4	petD	4	TGGATGTTTCCCCTCAACTT	CGTTTCAAAGCGAATTCTCCC	20	21	45.0	47.6	56.6	58.1	0.0	3.5	2.517983	78936	80172	1404-1404	1.000000	59.9
petD_p5	petD	5	TTGGATGTTTCCCCTCAACT	CGTTTCAAAGCGAATTCTCCC	20	21	45.0	47.6	56.6	58.1	0.0	3.5	2.517983	78936	80172	1405-1405	1.000000	59.9