primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	AGAAGTTCCATCTACAAATGG	20	21	55.0	38.1	58.1	53.2	0.0	0.0	5.919867	76	456	405-430	1.000000	51.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	AGAAGTTCCATCTACAAATGG	20	21	50.0	38.1	57.9	53.2	0.0	0.0	5.924228	76	456	419-444	1.000000	51.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	AGAAGTTCCATCTACAAATGG	20	21	50.0	38.1	57.9	53.2	0.0	0.0	5.925736	76	456	418-443	1.000000	51.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	AGAAGTTCCATCTACAAATGG	20	21	50.0	38.1	57.9	53.2	0.0	0.0	5.925736	76	456	420-445	1.000000	51.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	AGAAGTTCCATCTACAAATGG	20	21	50.0	38.1	57.5	53.2	6.4	0.0	6.271171	76	456	434-459	1.000000	51.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCGGTCTTACAAACTC	TTGAAAAAGGTGCTCAGCCT	20	20	50.0	45.0	57.9	57.9	6.1	0.0	0.133517	4429	5547	1317-1331	1.000000	85.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCGCGGTCTTACAAACTCT	TTGAAAAAGGTGCTCAGCCT	20	20	50.0	45.0	57.9	57.9	6.1	0.0	0.133517	4429	5547	1316-1330	1.000000	85.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GTCGCGGTCTTACAAACTCT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.9	57.9	6.1	18.8	0.137719	4429	5547	1508-1522	1.000000	84.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCGGTCTTACAAACTC	TTTTCTTGAGCCGTACGAGG	20	20	50.0	50.0	57.9	57.9	6.1	18.8	0.136287	4429	5547	1518-1532	1.000000	84.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GTCGCGGTCTTACAAACTCT	TTTTCTTGAGCCGTACGAGG	20	20	50.0	50.0	57.9	57.9	6.1	18.8	0.136287	4429	5547	1517-1531	1.000000	84.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ACGTTGCCTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.170863	6716	7671	874-1048	1.000000	87.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GTTGCCTTCTACCACATCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.170863	6716	7671	872-1046	1.000000	87.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CACGTTGCCTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.267690	6716	7671	875-1049	1.000000	86.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6716	7671	896-1070	1.000000	85.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6716	7671	895-1069	1.000000	85.2
atpA-atpF_p1	atpA-atpF	1	ATGCCATCGCCTACTTGAAG	TGGCATGTTTGGAACCATGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.047764	12705	12763	212-214	1.000000	83.7
atpA-atpF_p2	atpA-atpF	2	ATGCCATCGCCTACTTGAAG	TGAAGTTCGACAACGGGTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.067962	12705	12763	317-319	1.000000	82.7
atpA-atpF_p3	atpA-atpF	3	ATGCCATCGCCTACTTGAAG	GCCCGCTTACGAAAAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041388	12705	12763	484-486	1.000000	82.5
atpA-atpF_p4	atpA-atpF	4	GTATTCCATTGCTCCCCGTT	GCCCGCTTACGAAAAGTAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.053212	12705	12763	1031-1033	1.000000	81.2
atpA-atpF_p5	atpA-atpF	5	GTATTCCATTGCTCCCCGTT	TGGCATGTTTGGAACCATGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.059588	12705	12763	759-761	1.000000	81.1
atpF-atpH_p1	atpF-atpH	1	GATGGCCAATAACCCAAGGA	TGGACTCGTTGTGGCATTAG	20	20	50.0	50.0	57.5	57.9	11.0	0.0	0.545907	14069	14545	563-581	1.000000	81.9
atpF-atpH_p2	atpF-atpH	2	GATGGCCAATAACCCAAGGA	TCGTTGTGGCATTAGCACTT	20	20	50.0	45.0	57.5	58.1	11.0	0.0	0.561724	14069	14545	558-576	1.000000	81.8
atpF-atpH_p3	atpF-atpH	3	GGATGGCCAATAACCCAAGG	TGGACTCGTTGTGGCATTAG	20	20	55.0	50.0	58.6	57.9	11.0	0.0	0.680188	14069	14545	564-582	1.000000	80.8
atpF-atpH_p4	atpF-atpH	4	GGATGGCCAATAACCCAAGG	TCGTTGTGGCATTAGCACTT	20	20	55.0	45.0	58.6	58.1	11.0	0.0	0.696006	14069	14545	559-577	1.000000	80.7
atpF-atpH_p5	atpF-atpH	5	TGGCCAATAACCCAAGGAAA	TGGACTCGTTGTGGCATTAG	20	20	45.0	50.0	57.3	57.9	0.6	0.0	0.797496	14069	14545	561-579	1.000000	79.9
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	30818	32085	1393-1430	1.000000	73.0
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30818	32085	1332-1369	1.000000	71.6
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	30818	32085	1393-1430	1.000000	68.8
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.6	56.1	0.0	0.0	2.280835	30818	32085	1332-1369	1.000000	67.5
petN-psbM_p5	petN-psbM	5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.1	57.6	0.0	0.0	2.300208	30818	32085	1394-1431	1.000000	67.1
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	37946	38197	402-417	1.000000	80.0
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	37946	38197	401-416	1.000000	79.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.481207	37946	38197	436-451	1.000000	79.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	37946	38197	413-428	1.000000	79.3
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.551451	37946	38197	447-462	1.000000	78.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CAAGACCGGAGCTATCAACC	CGACGTTGTTTTTCGACTGG	20	20	55.0	50.0	58.1	58.0	9.5	0.0	0.058852	38769	39483	1706-1860	1.000000	87.1
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CAAGACCGGAGCTATCAACC	CCTATACGCCTTCATCGACG	20	20	55.0	55.0	58.1	58.0	9.5	0.0	0.093592	38769	39483	1721-1875	1.000000	86.8
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GGCCATCTCTCCGAAAGAAG	CGACGTTGTTTTTCGACTGG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.053432	38769	39483	1825-1836	0.857143	77.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GGCCATCTCTCCGAAAGAAG	CTTGAGGTCACGGGTTCAAA	20	20	55.0	50.0	58.1	58.0	0.0	4.2	0.091462	38769	39483	1495-1504	0.857143	76.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GGCCATCTCTCCGAAAGAAG	CCTATACGCCTTCATCGACG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.088172	38769	39483	1840-1851	0.857143	76.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CTCTTTCTCGCCCGAAGTTG	AGGTGAAGGGTACGGAAAGA	20	20	55.0	50.0	58.9	58.0	0.0	0.0	0.948202	47757	48613	1058-1111	1.000000	77.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CTCTTTCTCGCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.9	58.1	0.0	0.0	1.022757	47757	48613	1026-1079	1.000000	77.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CTCTTTCTCGCCCGAAGTTG	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	58.9	58.2	0.0	0.0	1.079743	47757	48613	1047-1100	1.000000	76.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CTTTCTCGCCCGAAGTTGT	AGGTGAAGGGTACGGAAAGA	19	20	52.6	50.0	58.1	58.0	0.0	0.0	1.105428	47757	48613	1056-1109	1.000000	76.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCGCCCGAAGTTGTC	AGGTGAAGGGTACGGAAAGA	19	20	52.6	50.0	58.1	58.0	0.0	0.0	1.110991	47757	48613	1055-1108	1.000000	76.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CGATGACCCTCGCATTACAA	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.215809	50129	51356	1279-1306	1.000000	85.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.283137	50129	51356	1257-1284	1.000000	84.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAAGCCAGCTATCGGAGTC	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.467927	50129	51356	1303-1330	1.000000	83.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GACCCTCGCATTACAAATGC	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	57.5	57.8	13.4	0.0	0.625598	50129	51356	1275-1302	1.000000	82.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CTAACCTCTGAGCTAAGCGG	GCGTCTACCTATTTCGCCAT	20	20	55.0	50.0	57.5	57.8	5.5	0.0	0.650906	50129	51356	1249-1276	1.000000	82.0
ycf4-cemA_p1	ycf4-cemA	1	CAGAAAGCTGCTGAATTGGC	AACCCACGATTCAAGGCTTT	20	20	50.0	45.0	58.0	58.0	1.4	0.0	0.012454	64840	65710	1036-1084	0.285714	51.6
ycf4-cemA_p2	ycf4-cemA	2	CAGAAAGCTGCTGAATTGGC	ACCCACGATTCAAGGCTTTT	20	20	50.0	45.0	58.0	58.0	1.4	0.0	0.012454	64840	65710	1035-1083	0.285714	51.6
ycf4-cemA_p3	ycf4-cemA	3	AAATCGTCGCATCTTCCTCC	AACCCACGATTCAAGGCTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.063299	64840	65710	1202-1250	0.285714	51.1
ycf4-cemA_p4	ycf4-cemA	4	AAATCGTCGCATCTTCCTCC	ACCCACGATTCAAGGCTTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.063299	64840	65710	1201-1249	0.285714	51.1
ycf4-cemA_p5	ycf4-cemA	5	CATTCCCTTGACTCGTACCG	ACCCACGATTCAAGGCTTTT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.082053	64840	65710	1084-1132	0.285714	51.0
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	TTGTGATCGGGTTAATCGGT	21	20	42.9	45.0	57.2	56.9	5.1	0.0	2.825901	67579	68641	1133-1164	1.000000	63.9
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	CTTGTGATCGGGTTAATCGGT	21	21	42.9	47.6	57.2	58.1	5.1	0.0	2.861159	67579	68641	1134-1165	1.000000	63.6
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGTCCGA	TCTTGTGATCGGGTTAATCGG	21	21	42.9	47.6	57.2	57.8	5.1	2.2	2.936075	67579	68641	1135-1166	1.000000	63.0
petA-psbJ_p4	petA-psbJ	4	TGAGAAGGTTCAATTGTCCGAA	TTGTGATCGGGTTAATCGGT	22	20	40.9	45.0	57.9	56.9	5.1	0.0	3.143473	67579	68641	1133-1164	1.000000	61.3
petA-psbJ_p5	petA-psbJ	5	TTGAGAAGGTTCAATTGTCCGA	TTGTGATCGGGTTAATCGGT	22	20	40.9	45.0	57.9	56.9	5.1	0.0	3.143473	67579	68641	1134-1165	1.000000	61.3
ndhF-rpl32_p1	ndhF-rpl32	1	GGCGAAGATCTTTTGTTGCC	CTCTTGCTATTGGTCCGGTC	20	20	50.0	55.0	58.0	58.1	9.9	0.0	0.076063	117053	118389	2002-2094	0.857143	79.5
ndhF-rpl32_p2	ndhF-rpl32	2	GGCGAAGATCTTTTGTTGCC	CTTGCTATTGGTCCGGTCAA	20	20	50.0	50.0	58.0	57.9	9.9	13.5	0.125338	117053	118389	2000-2092	0.857143	79.1
ndhF-rpl32_p3	ndhF-rpl32	3	TAGGGCGTGTGAACCAAAAA	CTCTTGCTATTGGTCCGGTC	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.112907	117053	118389	2398-2490	0.857143	78.6
ndhF-rpl32_p4	ndhF-rpl32	4	GAAGTAAGCGGGTCAACCAA	CTCTTGCTATTGGTCCGGTC	20	20	50.0	55.0	58.1	58.1	0.9	0.0	0.171789	117053	118389	2171-2263	0.857143	78.4
ndhF-rpl32_p5	ndhF-rpl32	5	TAGGGCGTGTGAACCAAAAA	CTTGCTATTGGTCCGGTCAA	20	20	45.0	50.0	57.9	57.9	0.0	13.5	0.162182	117053	118389	2396-2488	0.857143	78.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGTAAATCTCTTTCTACAAGGA	AGCGTGTCTACCGATTTCAC	23	20	34.8	50.0	54.5	58.0	0.0	0.0	6.556856	118549	120312	1851-1983	1.000000	49.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGTAAATCTCTTTCTACAAGGA	CAGCGTGTCTACCGATTTCA	23	20	34.8	50.0	54.5	58.0	0.0	0.0	6.558227	118549	120312	1852-1984	1.000000	49.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGTAAATCTCTTTCTACAAGGA	TCCTAAGAGCAGCGTGTCTA	23	20	34.8	50.0	54.5	57.9	0.0	0.0	6.673303	118549	120312	1861-1993	1.000000	49.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGTAAATCTCTTTCTACAAGGA	CCTAAGAGCAGCGTGTCTAC	23	20	34.8	55.0	54.5	57.8	0.0	0.0	6.745321	118549	120312	1860-1992	1.000000	49.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGTAAATCTCTTTCTACAAGGA	CTAAGAGCAGCGTGTCTACC	23	20	34.8	55.0	54.5	57.8	0.0	0.0	6.745321	118549	120312	1859-1991	1.000000	49.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGCTCTAAAGTTGAAAATATCAT	20	23	50.0	26.1	57.9	52.2	0.0	0.0	8.956530	120393	120495	186-194	1.000000	47.3
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GTGAAATCGGTAGACACGCT	ATGCTCTAAAGTTGAAAATATCA	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.840083	120393	120495	197-205	1.000000	46.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	TGCTCTAAAGTTGAAAATATCAT	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.840083	120393	120495	196-204	1.000000	46.9
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	ATGCTCTAAAGTTGAAAATATCA	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.841454	120393	120495	196-204	1.000000	46.9
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TGAAATCGGTAGACACGCTG	TGCTCTAAAGTTGAAAATATCAT	20	23	50.0	26.1	58.0	52.2	0.0	0.0	8.841454	120393	120495	195-203	1.000000	46.9
ycf1_p1	ycf1	1	ACTCCGTTTCCATGTTGGTC	AAATTATCGCAAACCCCGGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.265739	131341	131460	1204-1219	1.000000	81.6
ycf1_p2	ycf1	2	ACTCCGTTTCCATGTTGGTC	AATTATCGCAAACCCCGGTT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.265739	131341	131460	1203-1218	1.000000	81.6
ycf1_p3	ycf1	3	TCGAGATTCGATACGGGACA	AAATTATCGCAAACCCCGGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.200280	131341	131460	860-860	0.857143	74.8
ycf1_p4	ycf1	4	TCGAGATTCGATACGGGACA	AATTATCGCAAACCCCGGTT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.200280	131341	131460	859-859	0.857143	74.8
ycf1_p5	ycf1	5	TCGAGATTCGATACGGGACA	CAAATTATCGCAAACCCCGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.244627	131341	131460	861-861	0.857143	74.5