primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	trnH-CAC-psbA	1	CTGCCTTAATCCACTTGGCT	GTAACGCTCACAACTTCCCT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.300108	77	393	376-478	1.000000	89.1
trnH-CAC-psbA_p2	trnH-CAC-psbA	2	ACTGCCTTAATCCACTTGGC	GTAACGCTCACAACTTCCCT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.325895	77	393	377-479	1.000000	88.9
trnH-CAC-psbA_p3	trnH-CAC-psbA	3	CTGCCTTAATCCACTTGGCT	TGAACGTAACGCTCACAACT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.347658	77	393	381-483	1.000000	88.6
trnH-CAC-psbA_p4	trnH-CAC-psbA	4	ACTGCCTTAATCCACTTGGC	TGAACGTAACGCTCACAACT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.373445	77	393	382-484	1.000000	88.4
trnH-CAC-psbA_p5	trnH-CAC-psbA	5	CTGCCTTAATCCACTTGGCT	ATGCATGAACGTAACGCTCA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.413984	77	393	386-488	1.000000	88.0
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCAGCTGTAAG	TTCTTCACGTCCCGGATTAC	20	20	50.0	50.0	57.9	57.3	16.6	0.7	0.716283	7045	7359	445-457	1.000000	79.1
psbK-psbI_p2	psbK-psbI	2	AGCAGCTGTAAGTTTTCGCT	TTCTTCACGTCCCGGATTAC	20	20	45.0	50.0	58.1	57.3	16.6	0.7	0.775953	7045	7359	437-449	1.000000	78.7
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCAGCTGTAAGTTT	TTCTTCACGTCCCGGATTAC	20	20	45.0	50.0	58.3	57.3	16.6	0.7	0.968992	7045	7359	442-454	1.000000	77.1
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCAGCTGTAAGTT	TTCTTCACGTCCCGGATTAC	20	20	45.0	50.0	58.3	57.3	16.6	0.7	0.968992	7045	7359	443-455	1.000000	77.1
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCAGCTGTAAGT	TTCTTCACGTCCCGGATTAC	20	20	45.0	50.0	58.3	57.3	16.6	0.7	0.968992	7045	7359	444-456	1.000000	77.0
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.699660	7650	8301	700-737	1.000000	57.4
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	2.0	0.0	3.738818	7650	8301	698-735	1.000000	57.1
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	57.8	56.2	0.0	0.0	3.940131	7650	8301	701-738	1.000000	55.5
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.6	55.5	0.9	0.0	3.945791	7650	8301	695-732	1.000000	55.5
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	AACGCTTTAGTCCACTCAGC	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.2	56.2	2.0	0.0	3.979289	7650	8301	699-736	1.000000	55.2
trnG-GGA-trnR-AGA_p1	trnG-GGA-trnR-AGA	1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.105791	9096	9253	279-284	1.000000	51.1
trnG-GGA-trnR-AGA_p2	trnG-GGA-trnR-AGA	2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.109107	9096	9253	285-290	1.000000	51.0
trnG-GGA-trnR-AGA_p3	trnG-GGA-trnR-AGA	3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	57.9	53.9	33.4	3.0	4.132047	9096	9253	299-304	1.000000	50.7
trnG-GGA-trnR-AGA_p4	trnG-GGA-trnR-AGA	4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.1	53.9	0.0	3.0	4.151221	9096	9253	287-292	1.000000	50.7
trnG-GGA-trnR-AGA_p5	trnG-GGA-trnR-AGA	5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	20	21	50.0	38.1	58.0	54.8	0.0	3.0	4.230351	9096	9253	280-285	1.000000	50.1
psbM-trnD-GAC_p1	psbM-trnD-GAC	1	TGAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	22	18	45.5	66.7	58.1	60.1	0.0	0.0	6.271365	28584	29546	1033-1043	1.000000	43.3
psbM-trnD-GAC_p2	psbM-trnD-GAC	2	GAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	21	18	47.6	66.7	56.4	60.1	0.0	0.0	6.682031	28584	29546	1032-1042	1.000000	43.3
psbM-trnD-GAC_p3	psbM-trnD-GAC	3	AGGAATGAGAATGAAGAGTGCA	GTTCGAGCCCCGTCAGTC	22	18	40.9	66.7	57.4	60.1	0.0	0.0	6.690813	28584	29546	1038-1048	1.000000	43.3
psbM-trnD-GAC_p4	psbM-trnD-GAC	4	TGAGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	22	18	45.5	66.7	58.1	60.7	0.0	0.0	6.889262	28584	29546	1064-1074	1.000000	43.2
psbM-trnD-GAC_p5	psbM-trnD-GAC	5	TGAGAATGAAGAGTGCAGTAGC	GCCCTGTCAAGGCGGAAG	22	18	45.5	66.7	58.1	60.7	0.0	0.0	6.893189	28584	29546	1057-1067	1.000000	43.2
trnE-GAA-trnT-ACC_p1	trnE-GAA-trnT-ACC	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30270	30802	666-686	1.000000	86.5
trnE-GAA-trnT-ACC_p2	trnE-GAA-trnT-ACC	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30270	30802	645-665	1.000000	86.3
trnE-GAA-trnT-ACC_p3	trnE-GAA-trnT-ACC	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30270	30802	642-662	1.000000	86.1
trnE-GAA-trnT-ACC_p4	trnE-GAA-trnT-ACC	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30270	30802	628-648	1.000000	86.1
trnE-GAA-trnT-ACC_p5	trnE-GAA-trnT-ACC	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30270	30802	607-627	1.000000	86.1
trnT-ACC-psbD_p1	trnT-ACC-psbD	1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.273069	30875	32091	1273-1304	1.000000	45.8
trnT-ACC-psbD_p2	trnT-ACC-psbD	2	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	20	20	55.0	40.0	58.1	53.0	7.6	6.7	5.054330	30875	32091	1296-1327	1.000000	45.7
trnT-ACC-psbD_p3	trnT-ACC-psbD	3	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	20	20	55.0	40.0	58.1	52.8	7.6	6.7	5.305690	30875	32091	1295-1326	1.000000	45.7
trnT-ACC-psbD_p4	trnT-ACC-psbD	4	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.1	53.0	0.0	6.7	5.076485	30875	32091	1299-1330	1.000000	45.6
trnT-ACC-psbD_p5	trnT-ACC-psbD	5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	20	20	50.0	40.0	58.1	52.8	0.0	6.7	5.327845	30875	32091	1298-1329	1.000000	45.6
ndhK-ndhC_p1	ndhK-ndhC	1	GGCTGGAAAGTCTTGACCAA	GGCGAAAGGGAGCATTAGAA	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.057130	49258	49320	187-196	1.000000	84.6
ndhK-ndhC_p2	ndhK-ndhC	2	GTTGTTCGATCAAGTACGGGA	GGCGAAAGGGAGCATTAGAA	21	20	47.6	50.0	58.0	58.0	9.1	0.0	1.087954	49258	49320	125-134	1.000000	78.7
ndhK-ndhC_p3	ndhK-ndhC	3	AGGCTGGAAAGTCTTGACCA	GGCGAAAGGGAGCATTAGAA	20	20	50.0	50.0	58.9	58.0	10.2	0.0	0.898075	49258	49320	188-197	1.000000	77.8
ndhK-ndhC_p4	ndhK-ndhC	4	ACCATAAAGAAGCGGCCATA	GGCGAAAGGGAGCATTAGAA	20	20	45.0	50.0	57.0	58.0	2.2	0.0	1.059612	49258	49320	207-216	1.000000	76.1
ndhK-ndhC_p5	ndhK-ndhC	5	GGCTGGAAAGTCTTGACCAA	TGGCGAAAGGGAGCATTAGA	20	20	50.0	50.0	58.0	59.1	11.3	0.0	1.109833	49258	49320	188-197	1.000000	76.1
ycf4-cemA_p1	ycf4-cemA	1	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.376743	59649	60101	531-566	1.000000	83.3
ycf4-cemA_p2	ycf4-cemA	2	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	20	20	45.0	45.0	58.0	57.5	0.0	0.0	0.490807	59649	60101	524-559	1.000000	82.4
ycf4-cemA_p3	ycf4-cemA	3	TTGGCTTACTTCTTGCGTGT	GAGAGATCAACCAGGGCAAA	20	20	45.0	50.0	58.0	57.5	0.0	0.0	0.535105	59649	60101	530-565	1.000000	82.1
ycf4-cemA_p4	ycf4-cemA	4	TTGGCTTACTTCTTGCGTGT	AGAGAGAGATCAACCAGGGC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.543247	59649	60101	533-568	1.000000	82.0
ycf4-cemA_p5	ycf4-cemA	5	TTGGCTTACTTCTTGCGTGT	GCAGAGAGAGATCAACCAGG	20	20	45.0	55.0	58.0	57.4	0.0	0.0	0.638677	59649	60101	535-570	1.000000	81.2
psaJ-rpl33_p1	psaJ-rpl33	1	ACATATCTTTCCGTAGCGCC	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.8	57.0	2.3	0.0	1.141137	66134	66540	542-548	1.000000	75.4
psaJ-rpl33_p2	psaJ-rpl33	2	TAGCGCCGGTACTAAGTACT	AACATCTTTACCCTTGGCCA	20	20	50.0	45.0	57.7	57.0	2.3	0.0	1.322803	66134	66540	529-535	1.000000	74.1
psaJ-rpl33_p3	psaJ-rpl33	3	ACATATCTTTCCGTAGCGCC	GAACATCTTTACCCTTGGCCA	20	21	50.0	47.6	57.8	58.2	2.3	0.0	1.347678	66134	66540	543-549	1.000000	73.8
psaJ-rpl33_p4	psaJ-rpl33	4	ACATATCTTTCCGTAGCGCC	CGAACATCTTTACCCTTGGC	20	20	50.0	50.0	57.8	56.8	2.3	0.0	1.355349	66134	66540	544-550	1.000000	73.7
psaJ-rpl33_p5	psaJ-rpl33	5	ACATATCTTTCCGTAGCGCC	ACATCTTTACCCTTGGCCAT	20	20	50.0	45.0	57.8	56.8	2.3	6.1	1.375844	66134	66540	541-547	1.000000	73.5
petD-rpoA_p1	petD-rpoA	1	TGGTTAGGTATTGGAGCAACA	ATTGAACATTTTCACATAGAAGA	21	23	42.9	26.1	56.9	52.4	0.0	0.0	10.712610	76811	76996	306-309	1.000000	43.4
petD-rpoA_p2	petD-rpoA	2	TGGTTAGGTATTGGAGCAACA	TTGAACATTTTCACATAGAAGAT	21	23	42.9	26.1	56.9	52.4	0.0	0.0	10.712610	76811	76996	305-308	1.000000	43.4
petD-rpoA_p3	petD-rpoA	3	TGGTTAGGTATTGGAGCAACA	AATTGAACATTTTCACATAGAAGA	21	24	42.9	25.0	56.9	53.2	0.0	0.0	10.878325	76811	76996	307-310	1.000000	43.4
petD-rpoA_p4	petD-rpoA	4	TGGTTAGGTATTGGAGCAACA	ATTGAACATTTTCACATAGAAGAT	21	24	42.9	25.0	56.9	53.0	0.0	0.0	11.084578	76811	76996	306-309	1.000000	43.4
petD-rpoA_p5	petD-rpoA	5	AGGTATTGGAGCAACATTACCA	TTGAACATTTTCACATAGAAGAT	22	23	40.9	26.1	57.4	52.4	22.6	0.0	11.217142	76811	76996	300-303	1.000000	43.4
ndhF-rpl32_p1	ndhF-rpl32	1	GTGGAATGAAAGGGATGATCCA	ACGAATACGCTTTTTCGAGA	22	20	45.5	40.0	58.1	55.5	14.4	19.5	4.551159	111816	112675	669-964	1.000000	53.5
ndhF-rpl32_p2	ndhF-rpl32	2	AGTGGAATGAAAGGGATGATCC	ACGAATACGCTTTTTCGAGA	22	20	45.5	40.0	57.8	55.5	1.9	19.5	4.629497	111816	112675	670-965	1.000000	52.9
ndhF-rpl32_p3	ndhF-rpl32	3	TGGAATGAAAGGGATGATCCA	ACGAATACGCTTTTTCGAGA	21	20	42.9	40.0	56.7	55.5	14.4	19.5	4.785040	111816	112675	668-963	1.000000	51.6
ndhF-rpl32_p4	ndhF-rpl32	4	GTGGAATGAAAGGGATGATCCA	ACGAATACGCTTTTTCGAGAT	22	21	45.5	38.1	58.1	56.1	14.4	19.5	4.995100	111816	112675	669-964	1.000000	50.0
ndhF-rpl32_p5	ndhF-rpl32	5	AGTGGAATGAAAGGGATGATCC	ACGAATACGCTTTTTCGAGAT	22	21	45.5	38.1	57.8	56.1	1.9	19.5	5.073438	111816	112675	670-965	1.000000	49.9
rpl32-trnL-CUA_p1	rpl32-trnL-CUA	1	CTTTCTACAGGTAATTCAAAAAGT	AGCAGCGTGTCTACCAATTT	24	20	29.2	45.0	53.5	57.8	0.0	0.0	8.689645	112835	113295	541-903	1.000000	44.7
rpl32-trnL-CUA_p2	rpl32-trnL-CUA	2	CTTTCTACAGGTAATTCAAAAAGT	GAGCAGCGTGTCTACCAATT	24	20	29.2	50.0	53.5	58.3	0.0	0.0	8.777787	112835	113295	542-904	1.000000	44.7
rpl32-trnL-CUA_p3	rpl32-trnL-CUA	3	CTTTCTACAGGTAATTCAAAAAGT	TCCTAAGAGCAGCGTGTCTA	24	20	29.2	50.0	53.5	57.9	0.0	0.0	8.628918	112835	113295	548-910	1.000000	44.6
rpl32-trnL-CUA_p4	rpl32-trnL-CUA	4	CTTTCTACAGGTAATTCAAAAAGT	CCTAAGAGCAGCGTGTCTAC	24	20	29.2	55.0	53.5	57.8	0.0	0.0	8.700935	112835	113295	547-909	1.000000	44.6
rpl32-trnL-CUA_p5	rpl32-trnL-CUA	5	CTTTCTACAGGTAATTCAAAAAGT	CTAAGAGCAGCGTGTCTACC	24	20	29.2	55.0	53.5	57.8	0.0	0.0	8.700935	112835	113295	546-908	1.000000	44.6
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCTGCAATTGTGGCTTCT	GCAAATGACAAGGTCGAATCC	20	21	40.0	47.6	55.9	57.9	7.4	0.0	3.237790	114471	114683	364-388	1.000000	59.1
ccsA-ndhD_p2	ccsA-ndhD	2	TGGATATGCTATTTTGGCGTCA	GCAAATGACAAGGTCGAATCC	22	21	40.9	47.6	58.2	57.9	0.0	0.0	3.322183	114471	114683	326-350	1.000000	58.9
ccsA-ndhD_p3	ccsA-ndhD	3	TGCTATTTTGGCGTCAATCT	GCAAATGACAAGGTCGAATCC	20	21	40.0	47.6	55.7	57.9	1.9	0.0	3.408873	114471	114683	320-344	1.000000	58.4
ccsA-ndhD_p4	ccsA-ndhD	4	AATTCTGCAATTGTGGCTTCT	GCAAATGACAAGGTCGAATCC	21	21	38.1	47.6	56.7	57.9	7.4	0.0	3.458146	114471	114683	365-389	1.000000	57.3
ccsA-ndhD_p5	ccsA-ndhD	5	TGCTATTTTGGCGTCAATCTT	GCAAATGACAAGGTCGAATCC	21	21	38.1	47.6	56.5	57.9	1.9	0.0	3.628882	114471	114683	320-344	1.000000	56.6
ndhE-ndhG_p1	ndhE-ndhG	1	GGCACTCAAAACAAGTACATGT	AATTTCGGTGGCTCGTCAAT	22	20	40.9	45.0	57.4	58.2	2.7	1.4	2.746936	117132	117375	265-318	1.000000	65.9
ndhE-ndhG_p2	ndhE-ndhG	2	AGGCACTCAAAACAAGTACATG	AATTTCGGTGGCTCGTCAAT	22	20	40.9	45.0	57.2	58.2	0.0	1.4	3.014909	117132	117375	266-319	1.000000	63.7
ndhE-ndhG_p3	ndhE-ndhG	3	GGCACTCAAAACAAGTACATGT	TTTCGGTGGCTCGTCAATAA	22	20	40.9	45.0	57.4	57.5	2.7	1.4	3.027213	117132	117375	263-316	1.000000	63.6
ndhE-ndhG_p4	ndhE-ndhG	4	AGGCACTCAAAACAAGTACA	AATTTCGGTGGCTCGTCAAT	20	20	40.0	45.0	55.1	58.2	0.0	1.4	3.065586	117132	117375	266-319	1.000000	63.3
ndhE-ndhG_p5	ndhE-ndhG	5	GGCACTCAAAACAAGTACATGTT	AATTTCGGTGGCTCGTCAAT	23	20	39.1	45.0	58.1	58.2	10.6	1.4	3.246638	117132	117375	265-318	1.000000	61.9
ycf1_p1	ycf1	1	CCGTCCCCCAAACAGTTAAA	GGATCTCTCGCGGAAGAATC	20	20	50.0	55.0	58.0	57.9	0.0	6.1	0.074848	125980	126099	1200-1209	1.000000	84.5
ycf1_p2	ycf1	2	CCGTCCCCCAAACAGTTAAA	CTGGAAGAATTGGAAGGCGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129795	125980	126099	838-847	0.666667	70.3
ycf1_p3	ycf1	3	CCGTCCCCCAAACAGTTAAA	AAGGCGAAACAGACGAAACT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.066469	125980	126099	825-834	0.500000	61.7
ycf1_p4	ycf1	4	CCGTCCCCCAAACAGTTAAA	GACGAAACTGTACCAACGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.154530	125980	126099	814-823	0.500000	61.0
ycf1_p5	ycf1	5	CCGTCCCCCAAACAGTTAAA	TGGCTTTGATCCGTTATGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.137226	125980	126099	684-687	0.333333	52.4