primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCTTCGCTTCATCCA	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.1	58.3	0.0	7.6	0.373671	4333	5226	1021-1048	1.000000	81.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4333	5226	973-1000	1.000000	80.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4333	5226	972-999	1.000000	80.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4333	5226	988-1015	1.000000	79.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	5.3	7.6	0.596451	4333	5226	982-1009	1.000000	79.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6350	8090	1799-1840	1.000000	82.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6350	8090	1814-1855	1.000000	80.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6350	8090	1813-1854	1.000000	80.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6350	8090	1870-1911	1.000000	79.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6350	8090	1803-1844	1.000000	77.5
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	8698	9116	532-539	1.000000	83.2
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	8698	9116	533-540	1.000000	83.2
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	20	20	50.0	50.0	57.9	58.3	1.4	0.7	0.348988	8698	9116	534-541	1.000000	81.4
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	8698	9116	529-536	1.000000	81.2
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	8698	9116	530-537	1.000000	81.2
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATCTAATGATCCGGGGCGTA	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	58.1	58.0	0.0	9.8	0.105185	9228	9365	246-246	1.000000	81.1
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TATCTAATGATCCGGGGCGT	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	58.1	58.0	0.0	9.8	0.105185	9228	9365	247-247	1.000000	81.1
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176426	9228	9365	270-270	1.000000	80.4
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TATCTAATGATCCGGGGCGT	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176426	9228	9365	271-271	1.000000	80.4
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TCTAATGATCCGGGGCGTAA	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	58.3	58.0	0.0	9.8	0.331100	9228	9365	245-245	1.000000	79.3
atpF_p1	atpF	1	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCA	20	23	50.0	34.8	57.7	52.5	18.5	0.0	8.859885	12871	14148	1373-1374	1.000000	41.4
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	AGTCTATCTATAAGAGGAGATCA	20	23	45.0	34.8	57.4	52.5	9.6	0.0	9.176441	12871	14148	1385-1386	1.000000	41.4
atpF_p3	atpF	3	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCAT	20	24	50.0	33.3	57.7	53.1	18.5	0.0	9.223280	12871	14148	1373-1374	1.000000	41.4
atpF_p4	atpF	4	AATTTCGTCGGCTCGAATGG	AGTCTATCTATAAGAGGAGATCA	20	23	50.0	34.8	59.0	52.5	13.2	0.0	9.527470	12871	14148	1382-1383	1.000000	41.4
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	AGTCTATCTATAAGAGGAGATCAT	20	24	45.0	33.3	57.4	53.1	9.6	0.0	9.539836	12871	14148	1385-1386	1.000000	41.4
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.368120	14149	14521	484-490	1.000000	79.5
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	14149	14521	486-492	1.000000	78.2
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.804163	14149	14521	550-556	1.000000	75.7
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.821586	14149	14521	551-557	1.000000	75.6
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.821586	14149	14521	552-558	1.000000	75.6
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14768	15890	1205-1212	1.000000	77.3
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.9	58.5	0.0	0.0	0.630002	14768	15890	1261-1268	1.000000	76.7
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	14768	15890	1221-1228	1.000000	76.4
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.2	58.5	1.4	0.0	0.701648	14768	15890	1206-1213	1.000000	76.2
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.2	58.5	0.0	0.0	0.708039	14768	15890	1265-1272	1.000000	76.0
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.072037	29530	30208	766-793	1.000000	82.8
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	29530	30208	726-753	1.000000	82.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	29530	30208	720-747	1.000000	81.4
trnC-GCA-petN_p4	trnC-GCA-petN	4	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.257120	29530	30208	760-787	1.000000	81.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.4	58.0	14.7	0.0	0.361535	29530	30208	744-771	1.000000	80.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	57.2	58.5	0.0	5.4	2.256286	31267	32424	1084-1290	1.000000	68.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.9	58.5	0.0	5.4	2.569447	31267	32424	1085-1291	1.000000	65.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	31267	32424	1063-1269	1.000000	64.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGCAGAAAAGCAGTAGGAACT	GTTCAATTGGTCAGAGCACC	21	20	42.9	50.0	57.2	57.0	0.0	5.4	2.776439	31267	32424	1085-1291	1.000000	63.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.7	58.5	0.0	5.4	2.805641	31267	32424	1083-1289	1.000000	63.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	TGGTTGGTCCGTCAGAAAAG	TGGACCAGAGAATGCGATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066626	33707	35072	2337-2348	1.000000	80.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	ACGGGGAATACTTTCAGGGA	TGGACCAGAGAATGCGATTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067287	33707	35072	2358-2369	1.000000	80.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TGGTTGGTCCGTCAGAAAAG	AAATCCCCTTGTGCTTCAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109338	33707	35072	2192-2203	1.000000	80.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	ACGGGGAATACTTTCAGGGA	AAATCCCCTTGTGCTTCAGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109999	33707	35072	2213-2224	1.000000	80.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GCTCACACTAAGCTCGGAAA	TGGACCAGAGAATGCGATTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.108706	33707	35072	2309-2320	1.000000	80.5
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.0	57.6	0.0	4.1	0.403359	65223	66251	1174-1190	1.000000	78.1
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	65223	66251	1157-1173	1.000000	77.1
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	13.8	4.1	0.543756	65223	66251	1171-1187	1.000000	76.9
petA-psbJ_p4	petA-psbJ	4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.6	57.6	17.6	4.1	0.716678	65223	66251	1169-1185	1.000000	75.6
petA-psbJ_p5	petA-psbJ	5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.4	57.6	17.6	4.1	0.764949	65223	66251	1168-1184	1.000000	75.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	67027	68191	1302-1310	1.000000	81.4
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	67027	68191	1304-1312	1.000000	81.3
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	67027	68191	1225-1233	1.000000	80.6
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	67027	68191	1227-1235	1.000000	80.6
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	67027	68191	1311-1319	1.000000	80.4
rpl14-rpl16_p1	rpl14-rpl16	1	ACACATCAATTCTCGAGCCC	TGAAATGGGTGGAGTAACAGA	20	21	50.0	42.9	58.0	56.6	7.8	0.0	2.469094	83428	83566	298-305	1.000000	62.6
rpl14-rpl16_p2	rpl14-rpl16	2	TCGACGATTGCTAGCTCCTA	TGAAATGGGTGGAGTAACAGA	20	21	50.0	42.9	58.0	56.6	17.2	0.0	2.476084	83428	83566	328-335	1.000000	62.4
rpl14-rpl16_p3	rpl14-rpl16	3	CCGCTGTTATCCGCTACATT	TGAAATGGGTGGAGTAACAGA	20	21	50.0	42.9	58.1	56.6	0.0	0.0	2.573391	83428	83566	279-286	1.000000	62.0
rpl14-rpl16_p4	rpl14-rpl16	4	ACACATCAATTCTCGAGCCC	TGAAATGGGTGGAGTAACAGAA	20	22	50.0	40.9	58.0	57.3	7.8	0.0	2.745373	83428	83566	298-305	1.000000	60.4
rpl14-rpl16_p5	rpl14-rpl16	5	TCGACGATTGCTAGCTCCTA	TGAAATGGGTGGAGTAACAGAA	20	22	50.0	40.9	58.0	57.3	17.2	0.0	2.752362	83428	83566	328-335	1.000000	60.2
ndhF_p1	ndhF	1	GATGTCCTTGCCCACACTAG	CGAACAGGTTGGTCATCTCA	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.485512	112465	114711	2639-2659	1.000000	77.9
ndhF_p2	ndhF	2	GATGTCCTTGCCCACACTAG	ACGAACAGGTTGGTCATCTC	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.487204	112465	114711	2640-2660	1.000000	77.9
ndhF_p3	ndhF	3	GATGGAATCGCCCATTACGA	CGAACAGGTTGGTCATCTCA	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.619491	112465	114711	2805-2825	1.000000	76.7
ndhF_p4	ndhF	4	GATGGAATCGCCCATTACGA	ACGAACAGGTTGGTCATCTC	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.621184	112465	114711	2806-2826	1.000000	76.7
ndhF_p5	ndhF	5	ACATGCCGAAGTGATGGAAA	CGAACAGGTTGGTCATCTCA	20	20	45.0	50.0	57.8	57.5	0.0	0.0	0.654500	112465	114711	2722-2742	1.000000	76.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	116039	116843	932-937	1.000000	80.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	116039	116843	933-938	1.000000	80.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	116039	116843	942-947	1.000000	79.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.321366	116039	116843	921-926	1.000000	79.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.322736	116039	116843	922-927	1.000000	79.2
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.145058	121360	121744	515-535	1.000000	83.1
ndhG-ndhI_p2	ndhG-ndhI	2	AATACCACTCCCAGACCTCC	AATCAAATTGCTTTGGGGCG	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.216739	121360	121744	541-561	1.000000	82.3
ndhG-ndhI_p3	ndhG-ndhI	3	CCACTCCCAGACCTCCTAAT	AATCAAATTGCTTTGGGGCG	20	20	55.0	45.0	57.8	57.9	0.0	0.0	0.280196	121360	121744	537-557	1.000000	81.9
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	GGCGGTTACCAATGTCAGTA	20	20	50.0	50.0	58.0	57.6	0.0	1.1	0.437340	121360	121744	499-519	1.000000	80.9
ndhG-ndhI_p5	ndhG-ndhI	5	TACCACTCCCAGACCTCCTA	AATCAAATTGCTTTGGGGCG	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.407670	121360	121744	539-559	1.000000	80.8
ycf1_p1	ycf1	1	ATTTGAGAGAACGGATCCGC	ACCGTGGATTATACCAAGCA	20	20	50.0	45.0	58.1	56.6	33.6	0.0	1.444337	127350	127469	232-234	1.000000	77.5
ycf1_p2	ycf1	2	ATCCGCGATTTCCTTGGTTT	ACCGTGGATTATACCAAGCA	20	20	45.0	45.0	58.2	56.6	8.2	0.0	1.551474	127350	127469	218-220	1.000000	76.7
ycf1_p3	ycf1	3	CCGCGATTTCCTTGGTTTTG	ACCGTGGATTATACCAAGCA	20	20	50.0	45.0	58.3	56.6	8.2	0.0	1.688673	127350	127469	216-218	1.000000	75.6
ycf1_p4	ycf1	4	ATTTGAGAGAACGGATCCGC	AACCGTGGATTATACCAAGCA	20	21	50.0	42.9	58.1	57.4	33.6	0.0	1.696586	127350	127469	233-235	1.000000	75.5
ycf1_p5	ycf1	5	ATTTGAGAGAACGGATCCGC	ACCGTGGATTATACCAAGCAA	20	21	50.0	42.9	58.1	57.4	33.6	0.0	1.696586	127350	127469	232-234	1.000000	75.5