primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TTCACTTTGGTATGGATTGA	TTGTGCAGTACAATTCCATT	20	20	35.0	35.0	52.0	53.4	0.0	0.0	10.598748	1444	4344	2994-2994	0.500000	20.0
trnK-UUU_p2	trnK-UUU	2	TTTCACTTTGGTATGGATTGA	TTGTGCAGTACAATTCCATT	21	20	33.3	35.0	53.0	53.4	0.0	0.0	10.626011	1444	4344	2995-2995	0.500000	20.0
trnK-UUU_p3	trnK-UUU	3	TTTTCACTTTGGTATGGATTGA	TTGTGCAGTACAATTCCATT	22	20	31.8	35.0	53.9	53.4	0.0	0.0	10.742200	1444	4344	2996-2996	0.500000	20.0
trnK-UUU_p4	trnK-UUU	4	TTTTTCACTTTGGTATGGATTGA	TTGTGCAGTACAATTCCATT	23	20	30.4	35.0	54.7	53.4	0.0	0.0	10.935655	1444	4344	2997-2997	0.500000	20.0
trnK-UUU_p5	trnK-UUU	5	TTTTCACTTTGGTATGGATTG	TTGTGCAGTACAATTCCATT	21	20	33.3	35.0	52.3	53.4	0.0	0.0	11.349800	1444	4344	2996-2996	0.500000	20.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	AAGGAGCAGCGGATATAGGA	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.420204	3236	4307	1200-1207	1.000000	77.2
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGGAGCAGCGGATATAGGAA	ATCCGGGTTGCTAACTCAAC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.420204	3236	4307	1199-1206	1.000000	77.2
matK-trnK-UUU_p3	matK-trnK-UUU	3	AAGGAGCAGCGGATATAGGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.7	58.2	0.0	17.1	0.479199	3236	4307	1182-1189	1.000000	76.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	AGGAGCAGCGGATATAGGAA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.7	58.2	0.0	17.1	0.479199	3236	4307	1182-1189	1.000000	76.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGGAGCAGCGGATATAGGAA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.7	58.2	0.0	17.1	0.479199	3236	4307	1181-1188	1.000000	76.7
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	AACGGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.640998	7941	8557	724-733	1.000000	68.4
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	AACGGAAAGACCCCTTAACT	20	20	50.0	45.0	58.0	56.4	0.0	0.0	1.642386	7941	8557	723-732	1.000000	68.4
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	GATCAGGGGAATCGGAGAGA	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.0	56.4	0.0	0.0	1.638921	7941	8557	789-798	1.000000	68.3
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATCAGGGGAATCGGAGAGAG	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.0	56.4	0.0	0.0	1.644658	7941	8557	788-797	1.000000	68.3
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	CAAGTGATCAGGGGAATCGG	AACGGAAAGACCCCTTAACT	20	20	55.0	45.0	58.0	56.4	0.0	0.0	1.677374	7941	8557	794-803	1.000000	68.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	57.5	0.0	14.6	0.698720	26088	27348	1397-1409	1.000000	75.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	20	20	50.0	55.0	58.2	58.6	0.0	16.2	0.823422	26088	27348	1421-1433	1.000000	74.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.2	58.8	0.0	0.0	0.938794	26088	27348	1434-1446	1.000000	73.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.4	57.5	0.0	14.6	1.111753	26088	27348	1398-1410	1.000000	72.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	20	21	50.0	47.6	58.2	58.0	0.0	14.6	1.205880	26088	27348	1398-1410	1.000000	71.5
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	GGACAGGCTTGGTTTGATCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.185062	41067	41695	814-822	1.000000	79.4
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	TGGACAGGCTTGGTTTGATC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.185062	41067	41695	815-823	1.000000	79.4
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	20	20	50.0	50.0	57.9	58.2	0.0	18.7	0.305239	41067	41695	769-777	1.000000	78.5
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	20	20	50.0	50.0	57.9	58.2	0.0	18.7	0.305239	41067	41695	768-776	1.000000	78.5
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.9	57.7	0.0	19.9	0.369896	41067	41695	805-813	1.000000	77.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAAAAAGAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.0	0.0	0.063299	45621	46662	1123-1143	1.000000	81.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AGAAAAAGAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.0	0.0	0.128108	45621	46662	1131-1151	1.000000	80.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45621	46662	1072-1092	1.000000	80.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45621	46662	1080-1100	1.000000	80.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45621	46662	1104-1124	1.000000	79.6
trnL-UAA_p1	trnL-UAA	1	ACAAGATCAATGAAAGAGGTGGA	AGAGTGAGGAAGTAAAATGGGC	23	22	39.1	45.5	57.8	58.0	5.6	0.0	5.290238	46663	47402	808-827	1.000000	41.9
trnL-UAA_p2	trnL-UAA	2	ACAAGATCAATGAAAGAGGTGGA	GAGTGAGGAAGTAAAATGGGCT	23	22	39.1	45.5	57.8	58.0	5.6	0.0	5.290238	46663	47402	807-826	1.000000	41.9
trnL-UAA_p3	trnL-UAA	3	ACAAGATCAATGAAAGAGGTGGA	AGTGAGGAAGTAAAATGGGCT	23	21	39.1	42.9	57.8	56.9	5.6	0.0	5.345658	46663	47402	806-825	1.000000	41.9
trnL-UAA_p4	trnL-UAA	4	GACAAGATCAATGAAAGAGGTGG	AGAGTGAGGAAGTAAAATGGGC	23	22	43.5	45.5	57.4	58.0	5.6	0.0	5.601147	46663	47402	809-828	1.000000	41.9
trnL-UAA_p5	trnL-UAA	5	GACAAGATCAATGAAAGAGGTGG	GAGTGAGGAAGTAAAATGGGCT	23	22	43.5	45.5	57.4	58.0	5.6	0.0	5.601147	46663	47402	808-827	1.000000	41.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTCCCTAATCGGAGCTA	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.118655	49788	50624	968-984	1.000000	80.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCCTTCCCTAATCGGAGCTA	GTTCGAGTCCGTATAGCCCT	20	20	55.0	55.0	58.0	58.7	0.0	0.0	0.694471	49788	50624	953-969	1.000000	75.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCTTCCCTAATCGGAGCTA	CGAGAAAGTCTACGGTTCGA	20	20	55.0	50.0	58.0	57.1	0.0	0.0	0.899636	49788	50624	967-983	1.000000	74.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTCCCTAATCGGAGCTA	TTCGAGTCCGTATAGCCCTA	20	20	55.0	50.0	58.0	57.1	0.0	0.0	0.948042	49788	50624	952-968	1.000000	73.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTTTTCTGGCCCTTCCCTAA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.1	58.1	0.0	0.0	1.006836	49788	50624	977-993	1.000000	73.3
petA-psbJ_p1	petA-psbJ	1	GTTCAGTTGTACGAAATGAATTTCT	TATTCCGGGTTGGGTTCATC	25	20	32.0	50.0	56.6	57.3	17.5	0.0	7.096270	61808	62606	853-853	1.000000	40.4
petA-psbJ_p2	petA-psbJ	2	GTTCAGTTGTACGAAATGAATTTCT	CTATTCCGGGTTGGGTTCAT	25	20	32.0	50.0	56.6	57.3	17.5	0.0	7.103872	61808	62606	854-854	1.000000	40.4
petA-psbJ_p3	petA-psbJ	3	TCAGTTGTACGAAATGAATTTCT	TATTCCGGGTTGGGTTCATC	23	20	30.4	50.0	54.6	57.3	12.6	0.0	7.124769	61808	62606	851-851	1.000000	40.4
petA-psbJ_p4	petA-psbJ	4	TCAGTTGTACGAAATGAATTTCT	CTATTCCGGGTTGGGTTCAT	23	20	30.4	50.0	54.6	57.3	12.6	0.0	7.132371	61808	62606	852-852	1.000000	40.4
petA-psbJ_p5	petA-psbJ	5	GTTCAGTTGTACGAAATGAAT	TATTCCGGGTTGGGTTCATC	21	20	33.3	50.0	52.5	57.3	17.5	0.0	7.214827	61808	62606	853-853	1.000000	40.4
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.174567	70256	70906	831-831	1.000000	78.9
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.192541	70256	70906	795-795	1.000000	78.8
clpP-psbB_p3	clpP-psbB	3	CAAACTGCATCCTCCTCTCC	CCAGCCCAACCAGAAACTAG	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.209145	70256	70906	814-814	1.000000	78.6
clpP-psbB_p4	clpP-psbB	4	CCAAACTGCATCCTCCTCTC	CCAGCCCAACCAGAAACTAG	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.209145	70256	70906	815-815	1.000000	78.6
clpP-psbB_p5	clpP-psbB	5	CAAACTGCATCCTCCTCTCC	TGGACAGAAAGCAATCGACC	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.227119	70256	70906	778-778	1.000000	78.5
rpoA-rps11_p1	rpoA-rps11	1	TCCCACTGTAGTGTTCGAGT	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	58.0	58.1	10.8	9.9	0.145612	77683	77749	228-228	1.000000	79.2
rpoA-rps11_p2	rpoA-rps11	2	CCCACTGTAGTGTTCGAGTG	AGAGATGCAGCATTACGAGC	20	20	55.0	50.0	57.9	58.1	10.8	9.9	0.202726	77683	77749	227-227	1.000000	78.7
rpoA-rps11_p3	rpoA-rps11	3	TCGAGTGGATCCTGCTACTT	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	57.9	58.1	14.2	9.9	0.282130	77683	77749	214-214	1.000000	78.1
rpoA-rps11_p4	rpoA-rps11	4	TTCGAGTGGATCCTGCTACT	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	57.9	58.1	14.2	9.9	0.282130	77683	77749	215-215	1.000000	78.1
rpoA-rps11_p5	rpoA-rps11	5	TGTTCGAGTGGATCCTGCTA	AGAGATGCAGCATTACGAGC	20	20	50.0	50.0	58.2	58.1	14.2	9.9	0.289405	77683	77749	217-217	1.000000	78.1
rps11_p1	rps11	1	TTGAATCATTTCTTTCTCTTGAA	TGGATATCCCCATATCTTTCT	23	21	26.1	38.1	52.6	52.8	0.0	19.3	14.602455	77750	78166	511-511	1.000000	40.0
rps11_p2	rps11	2	ATTGAATCATTTCTTTCTCTTGA	TGGATATCCCCATATCTTTCT	23	21	26.1	38.1	52.3	52.8	0.0	19.3	14.819665	77750	78166	512-512	1.000000	40.0
rps11_p3	rps11	3	TGAATCATTTCTTTCTCTTGAAT	TGGATATCCCCATATCTTTCT	23	21	26.1	38.1	52.3	52.8	0.0	19.3	14.819665	77750	78166	510-510	1.000000	40.0
rps11_p4	rps11	4	TTGAATCATTTCTTTCTCTTGAA	ATGGATATCCCCATATCTTTCT	23	22	26.1	36.4	52.6	53.5	0.0	19.3	14.918306	77750	78166	512-512	1.000000	40.0
rps11_p5	rps11	5	TGAATCATTTCTTTCTCTTGAATT	TGGATATCCCCATATCTTTCT	24	21	25.0	38.1	53.2	52.8	0.0	19.3	14.982797	77750	78166	510-510	1.000000	40.0
rpl36-infA_p1	rpl36-infA	1	CCTACGAATCAGGCGACATT	TGATTCAACCAGGGGACGTA	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.578156	78433	78558	225-241	1.000000	80.7
rpl36-infA_p2	rpl36-infA	2	CCTACGAATCAGGCGACATT	CCGGCGCAGTTTTATACGTA	20	20	50.0	50.0	57.8	58.4	0.0	10.8	0.666328	78433	78558	285-301	1.000000	79.8
rpl36-infA_p3	rpl36-infA	3	CCTACGAATCAGGCGACATT	ATCCGGCGCAGTTTTATACG	20	20	50.0	50.0	57.8	58.8	0.0	10.8	1.010701	78433	78558	287-303	1.000000	77.0
rpl36-infA_p4	rpl36-infA	4	CCTACGAATCAGGCGACATT	TATGATTCAACCAGGGGACG	20	20	50.0	50.0	57.8	57.0	0.0	0.0	1.225453	78433	78558	227-243	1.000000	75.6
rpl36-infA_p5	rpl36-infA	5	CCTACGAATCAGGCGACATT	GATTCAACCAGGGGACGTAT	20	20	50.0	50.0	57.8	57.0	0.0	0.0	1.227525	78433	78558	224-240	1.000000	75.6
rpl14_p1	rpl14	1	AGACACAATCCATTCATCGGA	ACGCGGGTTTCTTTATCTCT	21	20	42.9	45.0	57.1	56.9	0.0	12.5	2.965496	79541	79909	558-558	1.000000	56.4
rpl14_p2	rpl14	2	AGACACAATCCATTCATCGGA	GCGGGTTTCTTTATCTCTGGA	21	21	42.9	47.6	57.1	57.7	0.0	0.0	3.174477	79541	79909	556-556	1.000000	54.6
rpl14_p3	rpl14	3	AGACACAATCCATTCATCGGA	AACGCGGGTTTCTTTATCTCT	21	21	42.9	42.9	57.1	57.7	0.0	12.7	3.242425	79541	79909	559-559	1.000000	54.3
rpl14_p4	rpl14	4	GAGACACAATCCATTCATCGGA	ACGCGGGTTTCTTTATCTCT	22	20	45.5	45.0	58.2	56.9	0.0	12.5	3.255961	79541	79909	559-559	1.000000	54.1
rpl14_p5	rpl14	5	GAGACACAATCCATTCATCGG	ACGCGGGTTTCTTTATCTCT	21	20	47.6	45.0	56.8	56.9	0.0	12.5	3.283054	79541	79909	559-559	1.000000	53.9
rpl16_p1	rpl16	1	AGAGATAAAGAAACCCGCGT	AGAACAATTCTAATTCTATAAGGTTGA	20	27	45.0	25.9	56.9	54.9	4.9	0.0	11.108235	80029	81602	1693-1705	1.000000	40.8
rpl16_p2	rpl16	2	TCCAGAGATAAAGAAACCCGC	AGAACAATTCTAATTCTATAAGGTTGA	21	27	47.6	25.9	57.7	54.9	0.0	0.0	11.317216	80029	81602	1696-1708	1.000000	40.8
rpl16_p3	rpl16	3	AGAGATAAAGAAACCCGCGT	GAACAATTCTAATTCTATAAGGTTGA	20	26	45.0	26.9	56.9	53.7	4.9	0.0	11.380276	80029	81602	1692-1704	1.000000	40.8
rpl16_p4	rpl16	4	AGAGATAAAGAAACCCGCGTT	AGAACAATTCTAATTCTATAAGGTTGA	21	27	42.9	25.9	57.7	54.9	4.9	0.0	11.385164	80029	81602	1693-1705	1.000000	40.8
rpl16_p5	rpl16	5	AGAGATAAAGAAACCCGCGT	AGAACAATTCTAATTCTATAAGGTTG	20	26	45.0	26.9	56.9	53.7	4.9	0.0	11.393457	80029	81602	1693-1705	1.000000	40.8
rpl16-rps3_p1	rpl16-rps3	1	CACGAGTCACACACTAAGCA	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.171587	81603	81761	300-302	1.000000	79.7
rpl16-rps3_p2	rpl16-rps3	2	TCACGAGTCACACACTAAGC	AGAGAAGGCAGAGTTCCCTT	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.454724	81603	81761	301-303	1.000000	77.4
rpl16-rps3_p3	rpl16-rps3	3	CACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.520932	81603	81761	285-287	1.000000	77.0
rpl16-rps3_p4	rpl16-rps3	4	CACGAGTCACACACTAAGCA	CCTTACAAACAATTCGCGCT	20	20	50.0	45.0	57.9	57.4	0.0	5.6	0.746175	81603	81761	284-286	1.000000	75.2
rpl16-rps3_p5	rpl16-rps3	5	TCACGAGTCACACACTAAGC	CCCTTACAAACAATTCGCGC	20	20	50.0	50.0	57.6	58.4	0.0	0.0	0.804069	81603	81761	286-288	1.000000	74.7
rps3-rpl22_p1	rps3-rpl22	1	ATCCTTTGGTTTTGCGAACC	TGAAAAAGTTCAGACCCCGG	20	20	45.0	50.0	57.2	58.0	13.3	0.0	0.836699	82410	82487	238-260	1.000000	78.3
rps3-rpl22_p2	rps3-rpl22	2	CCAAGTCTGAAACCAAGTGGA	TGAAAAAGTTCAGACCCCGG	21	20	47.6	50.0	58.1	58.0	3.8	0.0	1.093390	82410	82487	192-214	1.000000	76.4
rps3-rpl22_p3	rps3-rpl22	3	TCCAAGTCTGAAACCAAGTGG	TGAAAAAGTTCAGACCCCGG	21	20	47.6	50.0	58.1	58.0	0.0	0.0	1.093390	82410	82487	193-215	1.000000	76.4
rps3-rpl22_p4	rps3-rpl22	4	AATCCTTTGGTTTTGCGAACC	TGAAAAAGTTCAGACCCCGG	21	20	42.9	50.0	57.9	58.0	13.3	0.0	1.130582	82410	82487	239-261	1.000000	75.9
rps3-rpl22_p5	rps3-rpl22	5	CCAAGTCTGAAACCAAGTGG	TGAAAAAGTTCAGACCCCGG	20	20	50.0	50.0	56.5	58.0	0.0	0.0	1.475386	82410	82487	192-214	1.000000	73.4