primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	86	634	664-669	1.000000	83.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.307703	86	634	666-671	1.000000	83.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306133	86	634	678-683	1.000000	83.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	86	634	677-682	1.000000	83.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	86	634	679-684	1.000000	83.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAAATCAAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	7.8	0.0	0.089038	6125	7260	1274-1285	1.000000	82.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AGTCCCGGTTATTGAGACCA	GAAATCAAAATGGGGCGTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.061767	6125	7260	1777-1788	1.000000	82.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGTCCCGGTTATTGAGACCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.129117	6125	7260	1719-1730	1.000000	82.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGTCCCGGTTATTGAGACCA	AGCAGCTTGCCAAACAAAAG	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.108984	6125	7260	2278-2288	1.000000	82.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGTCCCGGTTATTGAGACCA	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.109110	6125	7260	2283-2293	1.000000	82.1
trnG-CGA-trnR-UCU_p1	trnG-CGA-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10015	10242	280-308	1.000000	73.9
trnG-CGA-trnR-UCU_p2	trnG-CGA-trnR-UCU	2	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10015	10242	286-314	1.000000	73.1
trnG-CGA-trnR-UCU_p3	trnG-CGA-trnR-UCU	3	CTAACGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.4	56.9	0.0	0.0	2.484101	10015	10242	269-297	1.000000	70.9
trnG-CGA-trnR-UCU_p4	trnG-CGA-trnR-UCU	4	TAACGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.4	56.9	0.0	0.0	2.488493	10015	10242	268-296	1.000000	70.9
trnG-CGA-trnR-UCU_p5	trnG-CGA-trnR-UCU	5	CTAACGATGCGGGTTCGATT	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.4	57.9	0.0	0.0	2.567429	10015	10242	275-303	1.000000	70.1
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.1	58.5	0.0	0.0	0.621503	13881	14850	1042-1056	1.000000	77.5
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	57.9	58.5	0.0	0.0	0.666168	13881	14850	1060-1074	1.000000	77.1
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	57.8	58.5	0.0	0.0	0.732109	13881	14850	1059-1073	1.000000	76.5
atpH-atpI_p4	atpH-atpI	4	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.3	58.5	0.0	0.0	0.779884	13881	14850	1054-1068	1.000000	76.2
atpH-atpI_p5	atpH-atpI	5	AACGGAAGCAGCAGAAATCA	CCGCGGCTTATATAGGTGAA	20	20	45.0	50.0	58.1	57.3	0.0	14.7	0.839924	13881	14850	1027-1041	1.000000	75.8
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.0	56.4	0.0	0.0	2.616493	29207	30161	1044-1068	1.000000	61.8
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.0	56.4	0.0	0.0	2.616493	29207	30161	1042-1066	1.000000	61.8
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.0	55.4	0.0	0.0	2.635092	29207	30161	1051-1075	1.000000	61.6
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.0	55.4	0.0	0.0	2.635092	29207	30161	1049-1073	1.000000	61.6
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	20	21	55.0	42.9	57.9	56.4	0.0	0.0	2.691409	29207	30161	1041-1065	1.000000	61.2
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	44792	45637	1018-1031	1.000000	74.2
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	44792	45637	1017-1030	1.000000	74.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.018457	44792	45637	1039-1052	1.000000	73.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.020450	44792	45637	1038-1051	1.000000	73.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	44792	45637	989-1002	1.000000	73.4
accD_p1	accD	1	AAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	22	22	27.3	40.9	52.1	57.5	0.0	0.0	10.432548	58186	59727	1587-1587	1.000000	41.5
accD_p2	accD	2	AAAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	23	22	26.1	40.9	53.0	57.5	0.0	0.0	10.554700	58186	59727	1588-1588	1.000000	41.5
accD_p3	accD	3	TAAAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGC	24	22	25.0	40.9	53.0	57.5	0.0	0.0	11.488405	58186	59727	1589-1589	1.000000	41.5
accD_p4	accD	4	AAAATAAAGAATTGTGGATCGA	CGAAAAATTGAACTTAGTGCGCT	22	23	27.3	39.1	52.1	58.8	0.0	9.7	11.682480	58186	59727	1587-1587	1.000000	41.5
accD_p5	accD	5	AAAATAAAGAATTGTGGATCGA	TCGAAAAATTGAACTTAGTGCGC	22	23	27.3	39.1	52.1	58.8	0.0	0.0	11.685548	58186	59727	1588-1588	1.000000	41.5
petG-trnW-CCA_p1	petG-trnW-CCA	1	CAATACAGGCGTGGTGATCA	GGTTCAAGTCCTACAGAGCG	20	20	50.0	55.0	58.0	58.0	11.7	0.0	0.025095	66721	66808	143-145	1.000000	82.0
petG-trnW-CCA_p2	petG-trnW-CCA	2	ACAATACAGGCGTGGTGATC	GGTTCAAGTCCTACAGAGCG	20	20	50.0	55.0	58.0	58.0	11.7	0.0	0.026507	66721	66808	144-146	1.000000	82.0
petG-trnW-CCA_p3	petG-trnW-CCA	3	CAATACAGGCGTGGTGATCA	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.0	58.0	11.7	0.0	0.059592	66721	66808	174-176	1.000000	81.4
petG-trnW-CCA_p4	petG-trnW-CCA	4	CAATACAGGCGTGGTGATCA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	11.7	2.6	0.038792	66721	66808	196-198	1.000000	81.3
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACAATACAGGCGTGGTGATC	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	11.7	2.6	0.040203	66721	66808	197-199	1.000000	81.3
psbB-psbT_p1	psbB-psbT	1	TCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	20	20	45.0	50.0	56.9	57.6	15.2	0.0	1.442173	74485	74675	322-327	1.000000	70.4
psbB-psbT_p2	psbB-psbT	2	TCCAAAAACTTGGGGATCCA	TGGAACTTTAGGCGGTTCTC	20	20	45.0	50.0	56.9	57.5	15.2	12.5	1.550800	74485	74675	324-329	1.000000	69.6
psbB-psbT_p3	psbB-psbT	3	TTCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	21	20	42.9	50.0	57.7	57.6	15.2	0.0	1.695448	74485	74675	323-328	1.000000	68.4
psbB-psbT_p4	psbB-psbT	4	TCCAAAAACTTGGGGATCCA	GGAACTTTAGGCGGTTCTCG	20	20	45.0	55.0	56.9	58.6	15.2	12.5	1.726296	74485	74675	323-328	1.000000	68.2
psbB-psbT_p5	psbB-psbT	5	TCCAAAAACTTGGGGATCCA	AGTTGGAACTTTAGGCGGTT	20	20	45.0	45.0	56.9	57.3	15.2	0.0	1.734616	74485	74675	327-332	1.000000	68.1
psbT-psbN_p1	psbT-psbN	1	TCGAGAACCGCCTAAAGTTC	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.6	57.7	0.0	0.0	0.643202	74778	74843	175-175	1.000000	77.1
psbT-psbN_p2	psbT-psbN	2	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.787020	74778	74843	127-127	1.000000	76.9
psbT-psbN_p3	psbT-psbN	3	TCGAGAACCGCCTAAAGTTC	GTTTTACCGGGTACGCCTTA	20	20	50.0	50.0	57.6	57.6	0.0	6.3	0.737384	74778	74843	183-183	1.000000	76.3
psbT-psbN_p4	psbT-psbN	4	TCGAGAACCGCCTAAAGTTC	TAAGTTTTACCGGGTACGCC	20	20	50.0	50.0	57.6	57.6	0.0	6.3	0.737384	74778	74843	186-186	1.000000	76.3
psbT-psbN_p5	psbT-psbN	5	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.5	57.7	0.0	0.0	0.751829	74778	74843	173-173	1.000000	76.3
rps19-rpl2_p1	rps19-rpl2	1	TGGTAGATTCACGAGACCAAG	CACAACCCCTTGGGGTTATC	21	20	47.6	55.0	57.1	58.2	0.0	35.0	2.021428	84583	84645	275-280	1.000000	65.6
rps19-rpl2_p2	rps19-rpl2	2	GGTAGATTCACGAGACCAAGT	CACAACCCCTTGGGGTTATC	21	20	47.6	55.0	57.1	58.2	0.0	35.0	2.023304	84583	84645	274-279	1.000000	65.6
rps19-rpl2_p3	rps19-rpl2	3	TGGTAGATTCACGAGACCAAG	TTGGGGTTATCCTGCACTTG	21	20	47.6	50.0	57.1	57.8	0.0	0.0	2.083133	84583	84645	266-271	1.000000	65.1
rps19-rpl2_p4	rps19-rpl2	4	GGTAGATTCACGAGACCAAGT	TTGGGGTTATCCTGCACTTG	21	20	47.6	50.0	57.1	57.8	0.0	0.0	2.085009	84583	84645	265-270	1.000000	65.1
rps19-rpl2_p5	rps19-rpl2	5	GATGGTAGATTCACGAGACCA	CACAACCCCTTGGGGTTATC	21	20	47.6	55.0	56.9	58.2	0.0	35.0	2.230197	84583	84645	277-282	1.000000	63.9
ndhF_p1	ndhF	1	AATACCCCTTTCGGATCGGA	CCAATACCCCCTCCTTTTCC	20	20	50.0	55.0	58.2	57.5	0.0	0.0	0.657015	109113	111371	2920-2927	1.000000	76.4
ndhF_p2	ndhF	2	ATACCCCTTTCGGATCGGAA	CCAATACCCCCTCCTTTTCC	20	20	50.0	55.0	58.2	57.5	11.4	0.0	0.657015	109113	111371	2919-2926	1.000000	76.4
ndhF_p3	ndhF	3	ATACCCCTTTCGGATCGGAA	CCCAATACCCCCTCCTTTTC	20	20	50.0	55.0	58.2	57.5	11.4	0.0	0.657015	109113	111371	2920-2927	1.000000	76.4
ndhF_p4	ndhF	4	TACCCCTTTCGGATCGGAAT	CCAATACCCCCTCCTTTTCC	20	20	50.0	55.0	58.2	57.5	15.6	0.0	0.657015	109113	111371	2918-2925	1.000000	76.4
ndhF_p5	ndhF	5	TACCCCTTTCGGATCGGAAT	CCCAATACCCCCTCCTTTTC	20	20	50.0	55.0	58.2	57.5	15.6	0.0	0.657015	109113	111371	2919-2926	1.000000	76.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CCCTTTCAACCGGGAATTCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	6.3	0.0	0.089958	112061	112852	509-889	1.000000	84.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CCCTTTCAACCGGGAATTCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.1	58.0	6.3	0.0	0.091329	112061	112852	510-890	1.000000	84.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CCCTTTCAACCGGGAATTCA	TTTAGGAGATGACATGCCGC	20	20	50.0	50.0	58.1	58.0	6.3	0.0	0.128569	112061	112852	574-954	1.000000	84.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CCCTTTCAACCGGGAATTCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	6.3	0.0	0.206405	112061	112852	519-899	1.000000	83.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCCCTTTCAACCGGGAATTC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.8	58.0	6.3	0.0	0.217520	112061	112852	510-890	1.000000	83.8
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	TCAAATTGCTTTAGGTCGGT	20	20	50.0	40.0	58.0	55.3	0.0	0.0	2.781970	117388	117735	487-499	1.000000	61.3
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	TCAAATTGCTTTAGGTCGGTT	20	21	50.0	38.1	58.0	56.1	0.0	0.0	2.975302	117388	117735	487-499	1.000000	59.8
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAATGAAAAGGCCGAAT	TCAAATTGCTTTAGGTCGGT	20	20	45.0	40.0	57.8	55.3	0.0	0.0	2.964721	117388	117735	550-562	1.000000	59.4
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	ATCAAATTGCTTTAGGTCGGT	20	21	50.0	38.1	58.0	55.8	0.0	0.0	3.197953	117388	117735	488-500	1.000000	58.0
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAATGAAAAGGCCGAAT	TCAAATTGCTTTAGGTCGGTT	20	21	45.0	38.1	57.8	56.1	0.0	0.0	3.158053	117388	117735	550-562	1.000000	57.9
rps15-ycf1_p1	rps15-ycf1	1	CAGACCCTCTGTTTTCTTCGT	GATCCGTTTGTACCCACGAT	21	20	47.6	50.0	57.9	57.7	0.0	13.9	1.418011	122045	122430	487-489	1.000000	71.1
rps15-ycf1_p2	rps15-ycf1	2	CAGACCCTCTGTTTTCTTCGT	AAGGATCCGTTTGTACCCAC	21	20	47.6	50.0	57.9	57.5	0.0	6.0	1.598002	122045	122430	490-492	1.000000	69.7
rps15-ycf1_p3	rps15-ycf1	3	CAGACCCTCTGTTTTCTTCGT	ATCCGTTTGTACCCACGATT	21	20	47.6	45.0	57.9	57.2	0.0	13.9	1.887253	122045	122430	486-488	1.000000	67.4
rps15-ycf1_p4	rps15-ycf1	4	AGACCCTCTGTTTTCTTCGT	GATCCGTTTGTACCCACGAT	20	20	45.0	50.0	56.4	57.7	0.0	13.9	1.901231	122045	122430	486-488	1.000000	67.3
rps15-ycf1_p5	rps15-ycf1	5	AGACCCTCTGTTTTCTTCGT	AAGGATCCGTTTGTACCCAC	20	20	45.0	50.0	56.4	57.5	0.0	6.0	2.081222	122045	122430	489-491	1.000000	65.8
ycf1_p1	ycf1	1	CATTCCTGACAGCCCTTTCA	AGGAACAAGAGGCATTCAGC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.373442	126917	127036	357-363	1.000000	81.9
ycf1_p2	ycf1	2	CATTCCTGACAGCCCTTTCA	GGGAGAATACCCTCACCCAT	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.383934	126917	127036	478-484	1.000000	81.1
ycf1_p3	ycf1	3	TCATTCCTGACAGCCCTTTC	AGGAACAAGAGGCATTCAGC	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.665318	126917	127036	358-364	1.000000	79.5
ycf1_p4	ycf1	4	CATTCCTGACAGCCCTTTCA	TCGGAAGTGAAGGGGACTAA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.519093	126917	127036	385-385	0.666667	63.6
ycf1_p5	ycf1	5	CATTCCTGACAGCCCTTTCA	TTCGGAAGTGAAGGGGACTA	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.519093	126917	127036	386-386	0.666667	63.6