primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ndhF	1	GCAGAATACCGTCACCCATT	TCGGGAAAATACGCCCAATT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.170163	110897	113143	2540-2540	0.500000	58.6
ndhF_p2	ndhF	2	GCAGAATACCGTCACCCATT	CTCGGGAAAATACGCCCAAT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.362835	110897	113143	2541-2541	0.500000	57.1
ndhF_p3	ndhF	3	GCAGAATACCGTCACCCATT	CAAACTCGGGAAAATACGCC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.576026	110897	113143	2545-2545	0.500000	55.4
ndhF_p4	ndhF	4	GCAGAATACCGTCACCCATT	AAACTCGGGAAAATACGCCC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.582090	110897	113143	2544-2544	0.500000	55.3
ndhF_p5	ndhF	5	GCAGAATACCGTCACCCATT	TGAGAACCCAACTTTCCGTT	20	20	50.0	45.0	58.0	57.3	0.0	0.0	0.759812	110897	113143	2603-2603	0.500000	53.9
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	CAAGAGGTCGTGTGAACCAA	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.140838	113144	114720	2598-2598	0.500000	58.9
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	CAAGAGGTCGTGTGAACCAA	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.142196	113144	114720	2599-2599	0.500000	58.9
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	CAAGAGGTCGTGTGAACCAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.176173	113144	114720	2127-2127	0.500000	58.6
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	CAAGAGGTCGTGTGAACCAA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.236900	113144	114720	2120-2120	0.500000	58.1
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	CAAGAGGTCGTGTGAACCAA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.1	57.8	0.0	0.0	0.248191	113144	114720	2125-2125	0.500000	58.0
ccsA_p1	ccsA	1	TTCGTTTTCAAGGTGGGGAG	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048516	114887	115849	2138-2138	0.500000	59.6
ccsA_p2	ccsA	2	TTCGTTTTCAAGGTGGGGAG	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.054179	114887	115849	2137-2137	0.500000	59.6
ccsA_p3	ccsA	3	TTCGTTTTCAAGGTGGGGAG	TTCTCGCCTGTTCACACAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.055599	114887	115849	1613-1613	0.500000	59.6
ccsA_p4	ccsA	4	TTCGTTTTCAAGGTGGGGAG	TGGTGCTGCACTCTTTTTCT	20	20	50.0	45.0	58.0	57.9	0.0	29.5	0.081478	114887	115849	2248-2248	0.500000	59.3
ccsA_p5	ccsA	5	TTCGTTTTCAAGGTGGGGAG	TTTTTGATTCTGGACCGCGA	20	20	50.0	45.0	58.0	58.1	0.0	8.2	0.138780	114887	115849	1910-1910	0.500000	58.9
ndhD_p1	ndhD	1	TTTGTGTGAACAGGCGAGAA	TGGTCCTGGCTCCTAAATCA	20	20	45.0	50.0	58.0	58.0	0.0	8.7	0.065844	116092	117621	2176-2176	0.500000	59.5
ndhD_p2	ndhD	2	TTTGTGTGAACAGGCGAGAA	GACCGAAGACTGTGTTGGTT	20	20	45.0	50.0	58.0	58.1	0.0	11.2	0.081793	116092	117621	1906-1906	0.500000	59.3
ndhD_p3	ndhD	3	TTTGTGTGAACAGGCGAGAA	CCGACCGATTTCTTGAGTGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.111544	116092	117621	1863-1863	0.500000	59.1
ndhD_p4	ndhD	4	TTGGAGTTGGGACCCAAAAG	TGGTCCTGGCTCCTAAATCA	20	20	50.0	50.0	57.9	58.0	27.9	8.7	0.115240	116092	117621	2458-2458	0.500000	59.1
ndhD_p5	ndhD	5	TTGGAGTTGGGACCCAAAAG	GACCGAAGACTGTGTTGGTT	20	20	50.0	50.0	57.9	58.1	27.9	11.2	0.131189	116092	117621	2188-2188	0.500000	59.0
psaC-ndhE_p1	psaC-ndhE	1	TTTGCCCACTGTACATTGCT	TACGTCGTGGTATGGGATCA	20	20	45.0	50.0	58.0	57.9	7.7	8.5	0.061728	117955	118214	1681-1681	0.500000	59.5
psaC-ndhE_p2	psaC-ndhE	2	AACCAACACAGTCTTCGGTC	TACGTCGTGGTATGGGATCA	20	20	50.0	50.0	58.1	57.9	0.0	8.5	0.106218	117955	118214	1096-1096	0.500000	59.2
psaC-ndhE_p3	psaC-ndhE	3	ACACTCAAGAAATCGGTCGG	TACGTCGTGGTATGGGATCA	20	20	50.0	50.0	57.9	57.9	0.0	8.5	0.135970	117955	118214	1139-1139	0.500000	58.9
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	TACGTCGTGGTATGGGATCA	20	20	50.0	50.0	58.1	57.9	0.0	8.5	0.181554	117955	118214	1047-1047	0.500000	58.5
psaC-ndhE_p5	psaC-ndhE	5	ACGCTCGAACACATTGTGTA	TACGTCGTGGTATGGGATCA	20	20	45.0	50.0	57.9	57.9	22.0	8.5	0.198691	117955	118214	1006-1006	0.500000	58.4
ndhE_p1	ndhE	1	TGATTTAGGAGCCAGGACCA	TTTCTCTTTGGACCGTTGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058926	118215	118520	908-908	0.500000	59.5
ndhE_p2	ndhE	2	AACCAACACAGTCTTCGGTC	TTTCTCTTTGGACCGTTGGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.074875	118215	118520	1178-1178	0.500000	59.4
ndhE_p3	ndhE	3	TGATTTAGGAGCCAGGACCA	TACGTCGTGGTATGGGATCA	20	20	50.0	50.0	58.0	57.9	0.0	8.5	0.090270	118215	118520	826-826	0.500000	59.3
ndhE_p4	ndhE	4	ACACTCAAGAAATCGGTCGG	TTTCTCTTTGGACCGTTGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104626	118215	118520	1221-1221	0.500000	59.2
ndhE_p5	ndhE	5	AACCAACACAGTCTTCGGTC	TACGTCGTGGTATGGGATCA	20	20	50.0	50.0	58.1	57.9	0.0	8.5	0.106218	118215	118520	1096-1096	0.500000	59.2
ndhG_p1	ndhG	1	TGATTTAGGAGCCAGGACCA	CAAACGAACGGTTACTTGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061501	118719	119249	1440-1440	0.500000	59.5
ndhG_p2	ndhG	2	TGATTTAGGAGCCAGGACCA	CCACATCACCCCCATTCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098786	118719	119249	1299-1299	0.500000	59.2
ndhG_p3	ndhG	3	TGATTTAGGAGCCAGGACCA	ACATCAGAACGTTTCCGAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.5	0.114872	118719	119249	1886-1886	0.500000	59.1
ndhG_p4	ndhG	4	TGATTTAGGAGCCAGGACCA	AACGGTTACTTGGCGCTAAT	20	20	50.0	45.0	58.0	57.9	0.0	6.7	0.138123	118719	119249	1434-1434	0.500000	58.9
ndhG_p5	ndhG	5	TGATTTAGGAGCCAGGACCA	ACGGTTACTTGGCGCTAATT	20	20	50.0	45.0	58.0	57.9	0.0	6.7	0.138123	118719	119249	1433-1433	0.500000	58.9
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAACGGTCCAAAGAGAAA	TGATTACCTTGTCTCACGCG	20	20	50.0	50.0	58.0	58.0	0.0	3.5	0.033541	119250	119611	1063-1063	0.500000	59.7
ndhG-ndhI_p2	ndhG-ndhI	2	TGATCCCATACCACGACGTA	TGATTACCTTGTCTCACGCG	20	20	50.0	50.0	57.9	58.0	0.0	3.5	0.064885	119250	119611	1145-1145	0.500000	59.5
ndhG-ndhI_p3	ndhG-ndhI	3	CGCAGCAGCTACAAAATACG	TGATTACCTTGTCTCACGCG	20	20	50.0	50.0	57.9	58.0	0.0	3.5	0.086356	119250	119611	958-958	0.500000	59.3
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAACGGTCCAAAGAGAAA	ACATCAGAACGTTTCCGAGG	20	20	50.0	50.0	58.0	57.9	0.0	0.5	0.104626	119250	119611	998-998	0.500000	59.2
ndhG-ndhI_p5	ndhG-ndhI	5	TGATCCCATACCACGACGTA	ACATCAGAACGTTTCCGAGG	20	20	50.0	50.0	57.9	57.9	0.0	0.5	0.135970	119250	119611	1080-1080	0.500000	58.9
ndhI_p1	ndhI	1	CCCAACGGTCCAAAGAGAAA	CTCTAGCCGAGTGTGAGAGA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.055599	119612	120103	1643-1643	0.500000	59.6
ndhI_p2	ndhI	2	CCCAACGGTCCAAAGAGAAA	TCTCTAGCCGAGTGTGAGAG	20	20	50.0	55.0	58.0	58.0	0.0	1.1	0.055599	119612	120103	1644-1644	0.500000	59.6
ndhI_p3	ndhI	3	GCCAAGTAACCGTTCGTTTG	CTCTAGCCGAGTGTGAGAGA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.058173	119612	120103	1111-1111	0.500000	59.5
ndhI_p4	ndhI	4	GCCAAGTAACCGTTCGTTTG	TCTCTAGCCGAGTGTGAGAG	20	20	50.0	55.0	58.0	58.0	0.0	1.1	0.058173	119612	120103	1112-1112	0.500000	59.5
ndhI_p5	ndhI	5	TGATCCCATACCACGACGTA	CTCTAGCCGAGTGTGAGAGA	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.086942	119612	120103	1725-1725	0.500000	59.3
ndhA_p1	ndhA	1	CGCGTGAGACAAGGTAATCA	ACCAATGCTACGAGCTTCTG	20	20	50.0	50.0	58.0	58.0	0.0	8.1	0.024937	120177	122315	2832-2832	0.500000	59.8
ndhA_p2	ndhA	2	CGCGTGAGACAAGGTAATCA	CAGGCTCTGGAAGGAATTCC	20	20	50.0	55.0	58.0	58.0	0.0	17.3	0.059089	120177	122315	2666-2666	0.500000	59.5
ndhA_p3	ndhA	3	CGCGTGAGACAAGGTAATCA	CCGGGGTTGCTGAATATCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.060535	120177	122315	2944-2944	0.500000	59.5
ndhA_p4	ndhA	4	CGCGTGAGACAAGGTAATCA	AACCGGGGTTGCTGAATATC	20	20	50.0	50.0	58.0	57.9	0.0	6.3	0.061983	120177	122315	2946-2946	0.500000	59.5
ndhA_p5	ndhA	5	CGCGTGAGACAAGGTAATCA	TTTTAACCGGGGTTGCTGAA	20	20	50.0	45.0	58.0	57.9	0.0	13.3	0.078035	120177	122315	2950-2950	0.500000	59.4
ndhH_p1	ndhH	1	AGCAGCCCTTAATCCACCTA	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	58.1	57.4	0.0	11.3	0.701214	122317	123498	2298-2298	0.500000	54.4
ndhH_p2	ndhH	2	AGCAGCCCTTAATCCACCTA	CATCTCAGAGAGGTTTGCGA	20	20	50.0	50.0	58.1	57.3	0.0	6.2	0.768691	122317	123498	1906-1906	0.500000	53.9
ndhH_p3	ndhH	3	AGCAGCCCTTAATCCACCTA	TCATCTCAGAGAGGTTTGCG	20	20	50.0	50.0	58.1	57.3	0.0	11.8	0.768691	122317	123498	1907-1907	0.500000	53.9
ndhH_p4	ndhH	4	GATTGAGCAGCAGCCCTTAA	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	58.2	57.4	0.0	11.3	0.839349	122317	123498	2306-2306	0.500000	53.3
ndhH_p5	ndhH	5	TTGAGCAGCAGCCCTTAATC	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	58.2	57.4	0.0	11.3	0.839349	122317	123498	2304-2304	0.500000	53.3
rps15_p1	rps15	1	TCCCAACGCGTTACATAAGG	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	58.0	57.4	26.1	11.3	0.607466	123596	123868	832-832	0.500000	55.1
rps15_p2	rps15	2	TTGATATTCAGCAACCCCGG	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	57.9	57.4	0.0	11.3	0.645863	123596	123868	1190-1190	0.500000	54.8
rps15_p3	rps15	3	GATATTCAGCAACCCCGGTT	GCTCGCGAAAGAAATCAACA	20	20	50.0	45.0	57.9	57.4	0.0	11.3	0.647311	123596	123868	1188-1188	0.500000	54.8
rps15_p4	rps15	4	AACACCATGCATTGATGGGT	GCTCGCGAAAGAAATCAACA	20	20	45.0	45.0	58.1	57.4	8.9	11.3	0.653151	123596	123868	695-695	0.500000	54.8
rps15_p5	rps15	5	TCAGCAACCCCGGTTAAAAA	GCTCGCGAAAGAAATCAACA	20	20	45.0	45.0	57.9	57.4	0.0	11.3	0.663363	123596	123868	1183-1183	0.500000	54.7
rps15-ycf1_p1	rps15-ycf1	1	TCCCAACGCGTTACATAAGG	AAACGACGGTGGACAATTGA	20	20	50.0	45.0	58.0	58.0	26.1	13.6	0.056640	123869	124224	1446-1446	0.500000	59.5
rps15-ycf1_p2	rps15-ycf1	2	TCCCAACGCGTTACATAAGG	CCATTCGTTTGCCTGGAAAC	20	20	50.0	50.0	58.0	57.9	26.1	0.0	0.077510	123869	124224	1462-1462	0.500000	59.4
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	AAACGACGGTGGACAATTGA	20	20	45.0	45.0	58.1	58.0	8.9	13.6	0.102326	123869	124224	1309-1309	0.500000	59.2
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	CCATTCGTTTGCCTGGAAAC	20	20	45.0	50.0	58.1	57.9	8.9	0.0	0.123196	123869	124224	1325-1325	0.500000	59.0
rps15-ycf1_p5	rps15-ycf1	5	AGCTGTTCCGGTCCATTTAC	AAACGACGGTGGACAATTGA	20	20	50.0	45.0	57.9	58.0	0.0	13.6	0.153128	123869	124224	1500-1500	0.500000	58.8
ycf1_p1	ycf1	1	CGAATTTTCAATCGTGGATAC	AAACGACGGTGGACAATTGA	21	20	38.1	45.0	53.4	58.0	0.0	13.6	5.616189	124278	124397	501-501	0.500000	20.0
ycf1_p2	ycf1	2	CGAATTTTCAATCGTGGATAC	CCATTCGTTTGCCTGGAAAC	21	20	38.1	50.0	53.4	57.9	0.0	0.0	5.637059	124278	124397	517-517	0.500000	20.0
ycf1_p3	ycf1	3	CGAATTTTCAATCGTGGATAC	AAAAAGAACCCACAGGCCAA	21	20	38.1	45.0	53.4	58.1	0.0	5.0	5.699713	124278	124397	883-883	0.500000	20.0
ycf1_p4	ycf1	4	CGAATTTTCAATCGTGGATAC	AACGACGGTGGACAATTGAT	21	20	38.1	45.0	53.4	57.8	0.0	13.6	5.751398	124278	124397	500-500	0.500000	20.0
ycf1_p5	ycf1	5	CGAATTTTCAATCGTGGATAC	ACGACGGTGGACAATTGATT	21	20	38.1	45.0	53.4	57.8	0.0	13.6	5.751398	124278	124397	499-499	0.500000	20.0
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TATGGTATCCCAGGCCTCTG	CCCCGTAACTCTTCTTCAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.041567	127735	129910	2953-2953	0.500000	59.7
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TATGGTATCCCAGGCCTCTG	CCCGTAACTCTTCTTCAGCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.041567	127735	129910	2952-2952	0.500000	59.7
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TATGGTATCCCAGGCCTCTG	GCCCCGTAACTCTTCTTCAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.041567	127735	129910	2954-2954	0.500000	59.7
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GTATGGTATCCCAGGCCTCT	CCCCGTAACTCTTCTTCAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.043035	127735	129910	2954-2954	0.500000	59.7
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GTATGGTATCCCAGGCCTCT	CCCGTAACTCTTCTTCAGCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.043035	127735	129910	2953-2953	0.500000	59.7