primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	CCACTTGGCTACATCCGC	GAGGCTCCATCTACAAATGG	18	20	61.1	50.0	58.2	55.6	0.0	2.1	4.594142	71	326	298-345	1.000000	55.2
trnH-psbA_p2	trnH-psbA	2	TCCACTTGGCTACATCCGC	GAGGCTCCATCTACAAATGG	19	20	57.9	50.0	59.8	55.6	0.0	2.1	5.186989	71	326	299-346	1.000000	52.0
trnH-psbA_p3	trnH-psbA	3	TCCACTTGGCTACATCCG	GAGGCTCCATCTACAAATGG	18	20	55.6	50.0	56.3	55.6	0.0	2.1	6.087001	71	326	299-346	1.000000	52.0
trnH-psbA_p4	trnH-psbA	4	CCACTTGGCTACATCCGC	AGGCTCCATCTACAAATGG	18	19	61.1	47.4	58.2	54.2	0.0	0.0	7.006302	71	326	297-344	1.000000	52.0
trnH-psbA_p5	trnH-psbA	5	TCCACTTGGCTACATCCGC	AGGCTCCATCTACAAATGG	19	19	57.9	47.4	59.8	54.2	0.0	0.0	7.599150	71	326	298-345	1.000000	52.0
psbA_p1	psbA	1	CTGCCTTAATCCACTTGGCT	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.513967	327	1388	1396-1408	0.500000	59.3
psbA_p2	psbA	2	ACTGCCTTAATCCACTTGGC	ATTTGTGTGCTTGGGAGTCC	20	20	50.0	50.0	58.2	58.4	0.0	0.0	0.539754	327	1388	1397-1409	0.500000	59.1
psbA_p3	psbA	3	CTGCCTTAATCCACTTGGCT	AAAATGCAAGCACGGTTTGG	20	20	50.0	45.0	57.9	58.4	0.0	0.0	0.553974	327	1388	1727-1733	0.500000	58.9
psbA_p4	psbA	4	ACTGCCTTAATCCACTTGGC	AAAATGCAAGCACGGTTTGG	20	20	50.0	45.0	58.2	58.4	0.0	0.0	0.579761	327	1388	1728-1734	0.500000	58.6
psbA_p5	psbA	5	CTGCCTTAATCCACTTGGCT	TTACGGGGGAAGACCAAGTT	20	20	50.0	50.0	57.9	58.6	0.0	0.0	0.697392	327	1388	2076-2082	0.500000	58.4
trnK-trnQ_p1	trnK-trnQ	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4285	6502	2255-2351	1.000000	86.8
trnK-trnQ_p2	trnK-trnQ	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4285	6502	2254-2350	1.000000	86.8
trnK-trnQ_p3	trnK-trnQ	3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4285	6502	2241-2337	1.000000	84.5
trnK-trnQ_p4	trnK-trnQ	4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	6.4	16.9	0.646638	4285	6502	2266-2362	1.000000	83.6
trnK-trnQ_p5	trnK-trnQ	5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4285	6502	2264-2360	1.000000	82.3
trnS-trnT_p1	trnS-trnT	1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.8	55.5	0.0	0.0	3.699660	7835	8451	682-785	1.000000	56.3
trnS-trnT_p2	trnS-trnT	2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	2.0	0.0	3.738818	7835	8451	680-783	1.000000	56.0
trnS-trnT_p3	trnS-trnT	3	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.6	55.5	0.9	0.0	3.945791	7835	8451	677-780	1.000000	54.4
trnS-trnT_p4	trnS-trnT	4	TCCAACGCTTTAGTCCACTC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.5	55.5	0.0	0.0	3.986452	7835	8451	683-786	1.000000	54.0
trnS-trnT_p5	trnS-trnT	5	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.5	55.5	0.0	0.0	4.051804	7835	8451	672-775	1.000000	53.6
trnT-trnR_p1	trnT-trnR	1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.105791	9246	9406	283-290	0.750000	40.8
trnT-trnR_p2	trnT-trnR	2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.0	53.9	0.0	3.0	4.109107	9246	9406	289-296	0.750000	40.7
trnT-trnR_p3	trnT-trnR	3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	57.9	53.9	33.4	3.0	4.132047	9246	9406	303-310	0.750000	40.3
trnT-trnR_p4	trnT-trnR	4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	20	20	50.0	40.0	58.1	53.9	0.0	3.0	4.151221	9246	9406	291-298	0.750000	40.3
trnT-trnR_p5	trnT-trnR	5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	20	21	50.0	38.1	58.0	54.8	0.0	3.0	4.230351	9246	9406	284-291	0.750000	39.8
trnR-atpA_p1	trnR-atpA	1	GCGAAAAGCGTCCATTGTC	GAACGTTTTCTACTTCAAGAGA	19	22	52.6	36.4	58.0	54.1	0.0	0.5	6.856688	9479	9791	397-422	1.000000	49.9
trnR-atpA_p2	trnR-atpA	2	GCGAAAAGCGTCCATTGTC	GAACGTTTTCTACTTCAAGAGAA	19	23	52.6	34.8	58.0	54.9	0.0	0.5	7.083541	9479	9791	397-422	1.000000	49.9
trnR-atpA_p3	trnR-atpA	3	GCGAAAAGCGTCCATTGTC	AACGTTTTCTACTTCAAGAGA	19	21	52.6	33.3	58.0	52.8	0.0	0.0	7.211209	9479	9791	396-421	1.000000	49.9
trnR-atpA_p4	trnR-atpA	4	GCGAAAAGCGTCCATTGTC	ACGTTTTCTACTTCAAGAGAA	19	21	52.6	33.3	58.0	52.8	0.0	0.0	7.211209	9479	9791	395-420	1.000000	49.9
trnR-atpA_p5	trnR-atpA	5	GCGAAAAGCGTCCATTGTC	ACGTTTTCTACTTCAAGAGAAA	19	22	52.6	31.8	58.0	53.7	0.0	7.5	7.331904	9479	9791	395-420	1.000000	49.9
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	20	20	45.0	50.0	58.1	58.0	0.0	5.7	0.127956	13374	13901	622-637	1.000000	87.8
atpH-atpI_p2	atpH-atpI	2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.152946	13374	13901	662-677	1.000000	87.3
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.136455	13374	13901	680-695	1.000000	87.2
atpH-atpI_p4	atpH-atpI	4	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	20	20	55.0	50.0	58.2	58.0	0.0	5.7	0.214492	13374	13901	684-699	1.000000	86.5
atpH-atpI_p5	atpH-atpI	5	AACGGAAGCAGCAGAAATCA	GGCTGCGGCTTATATAGGTG	20	20	45.0	55.0	58.1	58.3	0.0	18.8	0.376927	13374	13901	586-601	1.000000	86.1
ndhC-trnV_p1	ndhC-trnV	1	CTAGAAAGTTTCTCCGGCCC	AAATGCTCGGCAGAATGAGT	20	20	55.0	45.0	58.0	57.9	21.2	4.9	0.145390	50045	50797	1289-1307	0.750000	74.6
ndhC-trnV_p2	ndhC-trnV	2	CATTGCCCACGGATACAGAA	AAATGCTCGGCAGAATGAGT	20	20	50.0	45.0	58.0	57.9	0.0	4.9	0.156759	50045	50797	1413-1431	0.750000	74.2
ndhC-trnV_p3	ndhC-trnV	3	AACTCATTGCCCACGGATAC	AAATGCTCGGCAGAATGAGT	20	20	50.0	45.0	58.0	57.9	0.0	4.9	0.158188	50045	50797	1417-1435	0.750000	74.1
ndhC-trnV_p4	ndhC-trnV	4	GGCTGGAAAGTCTTGACCAA	AAATGCTCGGCAGAATGAGT	20	20	50.0	45.0	58.0	57.9	11.3	4.9	0.138545	50045	50797	1684-1706	0.750000	74.0
ndhC-trnV_p5	ndhC-trnV	5	GGATACCCCCAGTACATCGA	AAATGCTCGGCAGAATGAGT	20	20	55.0	45.0	58.0	57.9	0.0	4.9	0.125171	50045	50797	1443-1455	0.500000	62.8
trnW-trnP_p1	trnW-trnP	1	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	65464	65661	260-265	1.000000	86.8
trnW-trnP_p2	trnW-trnP	2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	65464	65661	262-267	1.000000	86.7
trnW-trnP_p3	trnW-trnP	3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	65464	65661	263-268	1.000000	86.7
trnW-trnP_p4	trnW-trnP	4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	65464	65661	273-278	1.000000	86.5
trnW-trnP_p5	trnW-trnP	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	65464	65661	274-279	1.000000	86.4
petB-petD_p1	petB-petD	1	GCAAGGTATTTCTGGTCCCT	CGGCCCGATCAATAGTTCAA	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.860802	75585	75781	257-257	1.000000	81.2
petB-petD_p2	petB-petD	2	AGCAAGGTATTTCTGGTCCC	CGGCCCGATCAATAGTTCAA	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.860802	75585	75781	258-258	1.000000	81.1
petB-petD_p3	petB-petD	3	AGCAAGGTATTTCTGGTCCC	GGCTTACACAGTGGTTGGAA	20	20	50.0	50.0	57.2	58.0	0.0	5.3	0.833246	75585	75781	333-333	1.000000	79.6
petB-petD_p4	petB-petD	4	GCAAGGTATTTCTGGTCCCT	GGCTTACACAGTGGTTGGAA	20	20	50.0	50.0	57.2	58.0	0.0	5.3	0.833246	75585	75781	332-332	1.000000	79.6
petB-petD_p5	petB-petD	5	GCAAGGTATTTCTGGTCCCTT	GGCTTACACAGTGGTTGGAA	21	20	47.6	50.0	57.9	58.0	0.0	5.3	1.111710	75585	75781	332-332	1.000000	77.4
rpl36-rps8_p1	rpl36-rps8	1	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	20	20	45.0	45.0	57.7	58.4	0.0	7.2	0.659243	78884	79365	519-542	1.000000	83.5
rpl36-rps8_p2	rpl36-rps8	2	TTTTACGAACGGAAGCCCTT	CGGCGGAGAAATTTTGTGTT	20	20	45.0	45.0	57.7	57.6	0.0	0.0	0.674964	78884	79365	518-541	1.000000	83.4
rpl36-rps8_p3	rpl36-rps8	3	TTTTACGAACGGAAGCCCTT	ATTCCACGGATTTTAGGCGG	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.583714	78884	79365	607-630	1.000000	82.9
rpl36-rps8_p4	rpl36-rps8	4	TTTTACGAACGGAAGCCCTT	CCACGGATTTTAGGCGGAAT	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.583714	78884	79365	604-627	1.000000	82.9
rpl36-rps8_p5	rpl36-rps8	5	ACGAACGGAAGCCCTTATTT	CCACGGATTTTAGGCGGAAT	20	20	45.0	50.0	57.5	58.3	0.0	0.0	0.809190	78884	79365	600-623	1.000000	81.2
rpl22-rps19_p1	rpl22-rps19	1	ACGTGCAATTCCCTCCATAC	CCGGATCGAAATGTTGGCTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.090283	83316	83375	1254-1260	1.000000	81.1
rpl22-rps19_p2	rpl22-rps19	2	TCATTGCTGCATCCAGGTTT	CCGGATCGAAATGTTGGCTA	20	20	45.0	50.0	58.1	58.1	1.8	0.0	0.124408	83316	83375	1023-1029	1.000000	81.1
rpl22-rps19_p3	rpl22-rps19	3	TTCATTGCTGCATCCAGGTT	CCGGATCGAAATGTTGGCTA	20	20	45.0	50.0	58.1	58.1	1.8	0.0	0.124408	83316	83375	1024-1030	1.000000	81.1
rpl22-rps19_p4	rpl22-rps19	4	TTTCATTGCTGCATCCAGGT	CCGGATCGAAATGTTGGCTA	20	20	45.0	50.0	58.1	58.1	1.8	0.0	0.124408	83316	83375	1025-1031	1.000000	81.1
rpl22-rps19_p5	rpl22-rps19	5	ACGTGCAATTCCCTCCATAC	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132749	83316	83375	1270-1276	1.000000	80.8
ndhF-rpl32_p1	ndhF-rpl32	1	AACAGGTCGTGTGAACCAAA	CCTGTCGAAAGTGATTTCCCT	20	21	45.0	47.6	57.9	57.9	0.0	1.0	1.176179	112046	112956	1482-1543	1.000000	79.1
ndhF-rpl32_p2	ndhF-rpl32	2	ACAGGTCGTGTGAACCAAAA	CCTGTCGAAAGTGATTTCCCT	20	21	45.0	47.6	57.9	57.9	0.0	1.0	1.176179	112046	112956	1481-1542	1.000000	79.1
ndhF-rpl32_p3	ndhF-rpl32	3	GGTCGTGTGAACCAAAAACC	CCTGTCGAAAGTGATTTCCCT	20	21	50.0	47.6	57.8	57.9	0.0	1.0	1.257144	112046	112956	1478-1539	1.000000	78.5
ndhF-rpl32_p4	ndhF-rpl32	4	CATGAAGAGGAAATTGCGCG	CCTGTCGAAAGTGATTTCCCT	20	21	50.0	47.6	58.2	57.9	0.0	1.0	1.240502	112046	112956	1714-1775	1.000000	78.3
ndhF-rpl32_p5	ndhF-rpl32	5	GAAATTGCGCGGATTTAGCA	CCTGTCGAAAGTGATTTCCCT	20	21	45.0	47.6	57.8	57.9	18.0	1.0	1.246703	112046	112956	1705-1766	1.000000	78.3
rpl32-trnL_p1	rpl32-trnL	1	AGTGCTTGTCTTCCAGTACAC	TGCAGACTGAGGCATTTTGT	21	20	47.6	45.0	57.9	58.0	0.0	6.7	1.127787	113116	114072	2049-2083	1.000000	79.4
rpl32-trnL_p2	rpl32-trnL	2	GTGCTTGTCTTCCAGTACACT	TGCAGACTGAGGCATTTTGT	21	20	47.6	45.0	57.9	58.0	0.0	6.7	1.127787	113116	114072	2048-2082	1.000000	79.4
rpl32-trnL_p3	rpl32-trnL	3	AGGGAAATCACTTTCGACAGG	TGCAGACTGAGGCATTTTGT	21	20	47.6	45.0	57.9	58.0	3.2	6.7	1.073879	113116	114072	1577-1597	1.000000	79.2
rpl32-trnL_p4	rpl32-trnL	4	GTGCTTGTCTTCCAGTACACT	TAACCACTGGGACTGGAGAG	21	20	47.6	55.0	57.9	58.1	0.0	0.0	1.174675	113116	114072	2081-2115	1.000000	78.9
rpl32-trnL_p5	rpl32-trnL	5	AGGGAAATCACTTTCGACAGG	TAACCACTGGGACTGGAGAG	21	20	47.6	55.0	57.9	58.1	3.2	0.0	1.120767	113116	114072	1610-1630	1.000000	78.7
ycf1_p1	ycf1	1	GGAGGCCCAAATGGTAGTTT	TGATTTTCGTCGAGCGCTTA	20	20	50.0	45.0	57.8	58.0	0.0	13.3	0.246382	126542	126661	687-687	0.250000	48.0
ycf1_p2	ycf1	2	TTCTGTTTCGGCTAGTGCAA	TGATTTTCGTCGAGCGCTTA	20	20	45.0	45.0	57.8	58.0	0.0	13.3	0.262946	126542	126661	493-493	0.250000	47.9
ycf1_p3	ycf1	3	GGAGGCCCAAATGGTAGTTT	GGATCTCTCGCGGAAGAATC	20	20	50.0	55.0	57.8	57.9	0.0	6.1	0.290630	126542	126661	1246-1246	0.250000	47.7
ycf1_p4	ycf1	4	GCATTTCACGTTCCCAATCC	TGATTTTCGTCGAGCGCTTA	20	20	50.0	45.0	57.7	58.0	0.0	13.3	0.292692	126542	126661	449-449	0.250000	47.7
ycf1_p5	ycf1	5	TTCTGTTTCGGCTAGTGCAA	GGATCTCTCGCGGAAGAATC	20	20	45.0	55.0	57.8	57.9	0.0	6.1	0.307194	126542	126661	1052-1052	0.250000	47.5