primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	76	442	485-487	1.000000	79.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.307703	76	442	487-489	1.000000	79.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306133	76	442	499-501	1.000000	79.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	76	442	498-500	1.000000	79.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.307640	76	442	500-502	1.000000	79.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCAAGAGCACCCTTATTCCC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.9	58.1	0.0	16.9	0.134694	6388	7086	786-843	1.000000	84.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6388	7086	732-789	1.000000	84.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6388	7086	715-772	1.000000	83.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6388	7086	733-790	1.000000	83.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGAGCACCCTTATTCCCAGA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.346535	6388	7086	783-840	1.000000	82.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8386	9113	786-797	1.000000	54.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8386	9113	785-796	1.000000	54.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	8386	9113	840-851	1.000000	53.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.780896	8386	9113	787-798	1.000000	52.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.782284	8386	9113	786-797	1.000000	52.6
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28041	28883	941-953	1.000000	81.7
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28041	28883	939-951	1.000000	81.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	28041	28883	993-1005	1.000000	81.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	28041	28883	991-1003	1.000000	81.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	28041	28883	942-954	1.000000	81.1
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	28974	29749	862-863	1.000000	78.0
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCCTACCGCCTTTCTGCTTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.441446	28974	29749	860-861	1.000000	78.0
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TCCTACCGCCTTTCTGCTTA	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.516362	28974	29749	859-860	1.000000	77.5
petN-psbM_p4	petN-psbM	4	ACTCGTAGTGTGGGGAAGAA	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	28974	29749	861-862	1.000000	76.6
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	CCTACCGCCTTTCTGCTTAT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.627434	28974	29749	859-860	1.000000	76.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGTGCTCTAACCGATTGAACT	TGGCAATATGTCTACGCTGG	21	20	47.6	50.0	58.0	57.8	0.0	0.0	1.264997	30452	30564	182-184	1.000000	72.9
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GGTGCTCTAACCGATTGAACT	GGCAATATGTCTACGCTGGT	21	20	47.6	50.0	58.0	57.8	0.0	0.0	1.266548	30452	30564	181-183	1.000000	72.9
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGTGCTCTAACCGATTGAACT	GGGGACGGACTGTAAATTCG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.377551	30452	30564	203-205	1.000000	71.7
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GGTGCTCTAACCGATTGAACT	GGGACGGACTGTAAATTCGT	21	20	47.6	50.0	58.0	57.6	0.0	0.0	1.401228	30452	30564	202-204	1.000000	71.5
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GGTGCTCTAACCGATTGAACT	GATTCTTCCTGGGTCGATGC	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.438508	30452	30564	236-238	1.000000	70.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	31440	32659	1371-1373	1.000000	79.4
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	ATAGTGACTCGTCCGGGAAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.314904	31440	32659	1417-1419	1.000000	79.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	31440	32659	1374-1376	1.000000	79.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	31440	32659	1370-1372	1.000000	78.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ATAGTGACTCGTCCGGGAAT	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.463380	31440	32659	1416-1418	1.000000	78.1
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	36230	36373	211-212	1.000000	81.5
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	36230	36373	212-213	1.000000	81.5
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	36230	36373	247-248	1.000000	81.3
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	36230	36373	248-249	1.000000	81.3
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	36230	36373	288-289	1.000000	80.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	GGTTTCCCACACTAAGGCTC	20	20	55.0	55.0	57.9	58.2	0.0	0.0	0.310234	45978	46667	791-795	1.000000	78.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	45978	46667	752-756	1.000000	78.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAGGAAAAAGCCGGCTATCG	GGTTTCCCACACTAAGGCTC	20	20	50.0	55.0	57.8	58.2	33.1	0.0	0.411794	45978	46667	842-846	1.000000	77.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	GGTTTCCCACACTAAGGCTC	20	20	50.0	55.0	57.8	58.2	15.5	0.0	0.417987	45978	46667	823-827	1.000000	77.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	45978	46667	803-807	1.000000	77.4
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62266	63325	1117-1118	1.000000	76.9
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	62266	63325	1119-1120	1.000000	76.9
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62266	63325	1116-1117	1.000000	76.5
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	62266	63325	1118-1119	1.000000	76.5
petA-psbJ_p5	petA-psbJ	5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	62266	63325	1116-1117	1.000000	76.1
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	21	20	47.6	55.0	57.6	57.7	0.0	0.0	1.680523	73123	73195	181-181	1.000000	68.3
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	21	20	47.6	55.0	57.6	58.4	0.0	0.0	1.824342	73123	73195	133-133	1.000000	67.8
psbT-psbN_p3	psbT-psbN	3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	73123	73195	189-189	1.000000	67.5
psbT-psbN_p4	psbT-psbN	4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	73123	73195	192-192	1.000000	67.5
psbT-psbN_p5	psbT-psbN	5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	21	20	47.6	55.0	57.6	57.5	0.0	4.1	1.945894	73123	73195	182-182	1.000000	66.2
ndhF-rpl32_p1	ndhF-rpl32	1	ACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	21	20	38.1	45.0	56.2	57.9	0.0	0.0	2.872219	110645	111148	641-648	1.000000	63.2
ndhF-rpl32_p2	ndhF-rpl32	2	ACGAAAGGTAAAATACACGCA	GCCCGATACCCCTTCTTTTT	21	20	38.1	50.0	56.2	57.9	0.0	0.0	2.932694	110645	111148	625-632	1.000000	62.9
ndhF-rpl32_p3	ndhF-rpl32	3	GAACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	23	20	39.1	45.0	58.0	57.9	0.0	0.0	3.099969	110645	111148	643-650	1.000000	61.4
ndhF-rpl32_p4	ndhF-rpl32	4	GAACGAAAGGTAAAATACACGCA	GCCCGATACCCCTTCTTTTT	23	20	39.1	50.0	58.0	57.9	0.0	0.0	3.160443	110645	111148	627-634	1.000000	61.1
ndhF-rpl32_p5	ndhF-rpl32	5	AACGAAAGGTAAAATACACGCA	AAATGCTTTTAACGCTGCCC	22	20	36.4	45.0	56.9	57.9	0.0	0.0	3.155681	110645	111148	642-649	1.000000	61.0
ccsA-ndhD_p1	ccsA-ndhD	1	CGGCCCTTTTATGTGGATCA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.075510	113186	113366	1082-1082	1.000000	80.9
ccsA-ndhD_p2	ccsA-ndhD	2	CGGCCCTTTTATGTGGATCA	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.081173	113186	113366	1081-1081	1.000000	80.9
ccsA-ndhD_p3	ccsA-ndhD	3	CGGCCCTTTTATGTGGATCA	TATCTTGACGAAATGGGCGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.103230	113186	113366	1137-1137	1.000000	80.7
ccsA-ndhD_p4	ccsA-ndhD	4	CGGCCCTTTTATGTGGATCA	TGACGAAATGGGCGGAATAG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.103230	113186	113366	1132-1132	1.000000	80.7
ccsA-ndhD_p5	ccsA-ndhD	5	GTTGGGACCCAAAGGAAACT	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.106316	113186	113366	761-761	1.000000	80.7
ycf1_p1	ycf1	1	TCATGTGAAACTTGGCTGCT	GAAATGGAAGGGGGCTTTGA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.077336	122137	122256	522-522	1.000000	82.8
ycf1_p2	ycf1	2	TCATGTGAAACTTGGCTGCT	GGAAGGGGGCTTTGAATCAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.077336	122137	122256	517-517	1.000000	82.8
ycf1_p3	ycf1	3	GTCATGTGAAACTTGGCTGC	GAAATGGAAGGGGGCTTTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.118903	122137	122256	523-523	1.000000	82.4
ycf1_p4	ycf1	4	GTCATGTGAAACTTGGCTGC	GGAAGGGGGCTTTGAATCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.118903	122137	122256	518-518	1.000000	82.4
ycf1_p5	ycf1	5	TCATGTGAAACTTGGCTGCT	AGTCGATATTGAGGCCTGGA	20	20	45.0	50.0	58.0	57.9	0.0	12.0	0.102631	122137	122256	1065-1065	1.000000	82.1
ycf15-ycf2_p1	ycf15-ycf2	1	GTTCGGGCAGTTCATACCAT	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	0.0	0.7	0.268516	142495	142592	257-262	1.000000	79.7
ycf15-ycf2_p2	ycf15-ycf2	2	GTTCGGGCAGTTCATACCAT	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	0.0	0.7	0.268516	142495	142592	256-261	1.000000	79.7
ycf15-ycf2_p3	ycf15-ycf2	3	TGGTTGTTCGCTGTTCAAGA	ATGGCTTTTCCCGGATGAAA	20	20	45.0	45.0	58.0	57.8	0.0	0.7	0.261388	142495	142592	283-288	1.000000	79.6
ycf15-ycf2_p4	ycf15-ycf2	4	TGGTTGTTCGCTGTTCAAGA	TGGCTTTTCCCGGATGAAAT	20	20	45.0	45.0	58.0	57.8	0.0	0.7	0.261388	142495	142592	282-287	1.000000	79.6
ycf15-ycf2_p5	ycf15-ycf2	5	TGGTTGTTCGCTGTTCAAGA	AAGATGGCTTTTCCCGGATG	20	20	45.0	50.0	58.0	58.2	0.0	0.7	0.263500	142495	142592	286-291	1.000000	79.6