primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	16178	16296	235-235	1.000000	80.8
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	16178	16296	234-234	1.000000	80.8
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	16178	16296	237-237	1.000000	80.8
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	16178	16296	236-236	1.000000	80.8
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	20	20	50.0	50.0	58.0	58.2	15.9	0.7	0.240071	16178	16296	238-238	1.000000	80.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	58.2	57.9	20.3	0.0	1.295845	28474	29047	698-726	1.000000	74.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	58.2	57.9	20.3	0.0	1.295845	28474	29047	697-725	1.000000	74.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	58.3	57.9	0.0	0.0	1.393263	28474	29047	712-740	1.000000	73.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	58.3	57.9	5.3	0.0	1.410232	28474	29047	707-735	1.000000	73.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	57.5	57.9	0.0	0.0	1.588183	28474	29047	684-712	1.000000	71.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	11.1	16.9	0.189961	30192	30788	691-697	1.000000	80.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	30192	30788	670-676	1.000000	80.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	30192	30788	681-687	1.000000	79.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	57.9	58.2	11.1	0.0	0.284881	30192	30788	721-727	1.000000	79.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	30192	30788	700-706	1.000000	79.3
atpF-atpH_p1	atpF-atpH	1	CCAGTGACCCAAGAAAACGA	GCAGAGGGAAAAATACGAGGT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.050974	36869	37292	580-590	1.000000	73.5
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.131081	36869	37292	607-617	1.000000	72.8
atpF-atpH_p3	atpF-atpH	3	CCAGTGACCCAAGAAAACGA	AGCAGAGGGAAAAATACGAGG	20	21	50.0	47.6	58.0	57.7	0.0	0.0	1.306229	36869	37292	581-591	1.000000	71.5
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	AGCAGAGGGAAAAATACGAGG	20	21	50.0	47.6	57.9	57.7	0.0	0.0	1.386336	36869	37292	608-618	1.000000	70.7
atpF-atpH_p5	atpF-atpH	5	CCAGTGACCCAAGAAAACGA	CGAAGCAGAGGGAAAAATACG	20	21	50.0	47.6	58.0	57.3	0.0	0.0	1.696558	36869	37292	584-594	1.000000	68.3
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	AAGCACCATGAAGAGCAACA	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.078594	54894	56172	1699-1710	1.000000	81.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	AGCACCATGAAGAGCAACAA	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.078594	54894	56172	1698-1709	1.000000	81.0
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	AAACTAGGCGCAAAGAACCA	20	20	55.0	45.0	58.1	58.0	7.6	10.8	0.088408	54894	56172	1859-1870	1.000000	80.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	AACTAGGCGCAAAGAACCAA	20	20	55.0	45.0	58.1	58.0	7.6	10.8	0.088408	54894	56172	1858-1869	1.000000	80.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	ACCAAAACTAGGCGCAAAGA	20	20	55.0	45.0	58.1	58.0	7.6	10.8	0.088408	54894	56172	1863-1874	1.000000	80.7
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	59893	60074	250-261	1.000000	83.7
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.277623	59893	60074	244-255	1.000000	83.3
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	59893	60074	243-254	1.000000	82.7
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	TCTTTGCCAAGGAGAAGACG	CACGGGTTCAAATCCTGTCT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.354836	59893	60074	242-253	1.000000	82.7
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.348744	59893	60074	248-259	1.000000	82.6
psaA-pafI_p1	psaA-pafI	1	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.525877	65206	66009	935-942	1.000000	77.5
psaA-pafI_p2	psaA-pafI	2	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.5	57.9	0.0	0.0	0.613689	65206	66009	869-876	1.000000	76.9
psaA-pafI_p3	psaA-pafI	3	TTTTACTTCTGGTTCCGGCG	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.626827	65206	66009	937-944	1.000000	76.7
psaA-pafI_p4	psaA-pafI	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	65206	66009	931-938	1.000000	75.7
psaA-pafI_p5	psaA-pafI	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	65206	66009	933-940	1.000000	75.7
pafI-trnS-GGA_p1	pafI-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	68051	68669	708-708	1.000000	72.3
pafI-trnS-GGA_p2	pafI-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	68051	68669	729-729	1.000000	71.8
pafI-trnS-GGA_p3	pafI-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	68051	68669	679-679	1.000000	71.5
pafI-trnS-GGA_p4	pafI-trnS-GGA	4	TGGCCACAATTGAAAAGGTC	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.8	58.1	13.6	0.0	1.291736	68051	68669	744-744	1.000000	71.0
pafI-trnS-GGA_p5	pafI-trnS-GGA	5	TGGCCACAATTGAAAAGGTC	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.8	58.2	13.6	0.0	1.348722	68051	68669	765-765	1.000000	70.5
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	71519	71865	413-414	1.000000	77.2
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTGAACTGGTGTAGTGGCAC	20	20	50.0	50.0	57.5	58.0	0.0	10.3	0.512616	71519	71865	494-494	0.333333	49.2
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GTGTAGTGGCACGAGGATTT	20	20	50.0	50.0	57.5	57.9	0.0	12.7	0.569172	71519	71865	486-486	0.333333	48.8
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TTTCAGTCCTCTGCTCTTGC	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.650287	71519	71865	468-468	0.333333	48.1
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	AGTGGCACGAGGATTTTCAG	20	20	50.0	50.0	57.5	58.2	0.0	12.7	0.660896	71519	71865	482-482	0.333333	48.0
psbE-petL_p1	psbE-petL	1	GAAACGGTAGGTACAGCCAG	CTCCTGCGGGTTAGAGAAAC	20	20	55.0	55.0	58.0	58.0	0.0	12.4	0.017511	88037	89280	2012-2016	1.000000	81.6
psbE-petL_p2	psbE-petL	2	CATTGCGGATATTGACCCCA	CTCCTGCGGGTTAGAGAAAC	20	20	50.0	55.0	58.0	58.0	0.0	12.4	0.031685	88037	89280	2037-2041	1.000000	81.5
psbE-petL_p3	psbE-petL	3	GAAACGGTAGGTACAGCCAG	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.0	58.0	0.0	2.6	0.021998	88037	89280	2164-2168	1.000000	81.4
psbE-petL_p4	psbE-petL	4	CAAGTGGTCGTGCTAGAGAC	CTCCTGCGGGTTAGAGAAAC	20	20	55.0	55.0	58.0	58.0	0.0	12.4	0.027568	88037	89280	2312-2316	1.000000	81.3
psbE-petL_p5	psbE-petL	5	CAAGTGGTCGTGCTAGAGAC	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	58.0	58.0	0.0	2.6	0.032055	88037	89280	2464-2468	1.000000	81.2
rpl16_p1	rpl16	1	GCACAAATAAATGCAAAGGGTG	TTTTCCACCCAATGAATTCT	22	20	40.9	35.0	57.3	52.9	0.0	7.1	7.782352	104526	105980	1634-1638	1.000000	42.3
rpl16_p2	rpl16	2	GCACAAATAAATGCAAAGGGT	TTTTCCACCCAATGAATTCT	21	20	38.1	35.0	55.9	52.9	0.0	7.1	8.210125	104526	105980	1634-1638	1.000000	42.3
rpl16_p3	rpl16	3	GCACAAATAAATGCAAAGGGTG	TTTTTCCACCCAATGAATTCT	22	21	40.9	33.3	57.3	53.8	0.0	7.1	7.846035	104526	105980	1635-1639	1.000000	42.2
rpl16_p4	rpl16	4	GCACAAATAAATGCAAAGGGT	TTTTTCCACCCAATGAATTCT	21	21	38.1	33.3	55.9	53.8	0.0	7.1	8.273808	104526	105980	1635-1639	1.000000	42.2
rpl16_p5	rpl16	5	GCACAAATAAATGCAAAGGGTG	TTTTTCCACCCAATGAATTC	22	20	40.9	35.0	57.3	52.1	0.0	0.0	8.522581	104526	105980	1635-1639	1.000000	42.2
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	115689	115807	235-235	1.000000	80.8
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	115689	115807	234-234	1.000000	80.8
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	115689	115807	237-237	1.000000	80.8
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	115689	115807	236-236	1.000000	80.8
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.2	58.0	0.7	15.9	0.240071	115689	115807	238-238	1.000000	80.8
ycf1_p1	ycf1	1	GGAATCGTCCAACGCGATAT	CTTTGAACACGCATGGAACC	20	20	50.0	50.0	58.2	57.9	15.9	0.0	0.270908	132102	132221	1051-1054	1.000000	80.6
ycf1_p2	ycf1	2	GGAATCGTCCAACGCGATAT	CTGGTTGCTCCGATGTATCC	20	20	50.0	55.0	58.2	58.1	15.9	0.0	0.349350	132102	132221	972-975	1.000000	80.1
ycf1_p3	ycf1	3	GGAATCGTCCAACGCGATAT	ATTAGGGGCCGGATTATCCA	20	20	50.0	50.0	58.2	57.9	15.9	5.0	0.274177	132102	132221	947-947	0.333333	51.1
ycf1_p4	ycf1	4	GGAATCGTCCAACGCGATAT	AATTTGATGCTCCGTCGACA	20	20	50.0	45.0	58.2	57.9	15.9	17.2	0.313737	132102	132221	875-875	0.333333	50.8
ycf1_p5	ycf1	5	GGAATCGTCCAACGCGATAT	GGGCCGGATTATCCAGATTC	20	20	50.0	55.0	58.2	57.9	15.9	5.0	0.335773	132102	132221	942-942	0.333333	50.6
ndhD-ccsA_p1	ndhD-ccsA	1	CCCGGATTTCGTTCTCTCAC	ATGGTTCATTTACATTAACACTT	20	23	55.0	26.1	58.4	52.6	0.7	0.0	8.759943	144319	144525	304-306	1.000000	43.2
ndhD-ccsA_p2	ndhD-ccsA	2	GGTATTGGCATTTACCCGGA	ATGGTTCATTTACATTAACACTT	20	23	50.0	26.1	57.6	52.6	0.7	0.0	8.753226	144319	144525	318-320	1.000000	43.0
ndhD-ccsA_p3	ndhD-ccsA	3	AGGTATTGGCATTTACCCGG	ATGGTTCATTTACATTAACACTT	20	23	50.0	26.1	57.6	52.6	18.5	0.0	8.759842	144319	144525	319-321	1.000000	43.0
ndhD-ccsA_p4	ndhD-ccsA	4	GGTATTGGCATTTACCCGGA	AGTTATGGTTCATTTACATTAACACT	20	26	50.0	26.9	57.6	55.4	0.7	0.0	8.915718	144319	144525	322-324	1.000000	43.0
ndhD-ccsA_p5	ndhD-ccsA	5	AGGTATTGGCATTTACCCGG	AGTTATGGTTCATTTACATTAACACT	20	26	50.0	26.9	57.6	55.4	18.5	0.0	8.922334	144319	144525	323-325	1.000000	43.0
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	CTCGCACTGCTTCCTAAGAG	AAAAAGGGGTATTGGGAGGC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.327781	145725	146722	1108-1150	1.000000	82.9
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CTCTCGCACTGCTTCCTAAG	AAAAAGGGGTATTGGGAGGC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.327781	145725	146722	1110-1152	1.000000	82.9
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	ATCCGCATAAAAGAGCTGCA	AAAAAGGGGTATTGGGAGGC	20	20	45.0	50.0	58.0	57.8	1.0	0.0	0.290385	145725	146722	1742-1790	1.000000	81.8
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	TCCGCATAAAAGAGCTGCAT	AAAAAGGGGTATTGGGAGGC	20	20	45.0	50.0	58.0	57.8	1.0	0.0	0.290385	145725	146722	1741-1789	1.000000	81.8
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GTTAAGAGGCCTGACGTAGC	AAAAAGGGGTATTGGGAGGC	20	20	55.0	50.0	58.1	57.8	12.0	0.0	0.323800	145725	146722	1686-1686	0.333333	50.7