primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	78	473	514-514	1.000000	76.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	473	528-528	1.000000	76.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	473	527-527	1.000000	76.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	473	529-529	1.000000	76.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	78	473	515-515	1.000000	75.8
psbA_p1	psbA	1	GAGCAATAACGCCCTCTTGA	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.0	58.2	0.0	9.7	0.259325	474	1535	1203-1203	1.000000	77.9
psbA_p2	psbA	2	AAAAGGAGCAATAACGCCCT	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	57.8	58.2	0.0	9.7	0.451091	474	1535	1208-1208	1.000000	76.4
psbA_p3	psbA	3	GAGCAATAACGCCCTCTTGA	ATATTGGTTGACACGGGCAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	474	1535	1268-1268	1.000000	76.3
psbA_p4	psbA	4	GAGCAATAACGCCCTCTTGA	ATTGGTTGACACGGGCATAT	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.459548	474	1535	1266-1266	1.000000	76.3
psbA_p5	psbA	5	AAAGGAGCAATAACGCCCTC	TTGAAAATTCGTGCGCTTGG	20	20	50.0	45.0	58.2	58.2	0.0	9.7	0.483712	474	1535	1207-1207	1.000000	76.1
matK_p1	matK	1	ACATACTACATCAACATTTCAGA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	53.5	57.6	0.0	0.0	7.867308	2065	3585	1681-1681	1.000000	40.0
matK_p2	matK	2	ACATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	24	20	29.2	50.0	54.3	57.6	0.0	0.0	8.080774	2065	3585	1681-1681	1.000000	40.0
matK_p3	matK	3	ACATACTACATCAACATTTCAGAAT	ACCCCCAAAATCCTCTACCT	25	20	28.0	50.0	54.8	57.6	0.0	0.0	8.549497	2065	3585	1681-1681	1.000000	40.0
matK_p4	matK	4	ACATACTACATCAACATTTCAGA	AACCCCCAAAATCCTCTACC	23	20	30.4	50.0	53.5	56.8	0.0	0.0	8.707648	2065	3585	1682-1682	1.000000	40.0
matK_p5	matK	5	CATACTACATCAACATTTCAGAA	ACCCCCAAAATCCTCTACCT	23	20	30.4	50.0	52.6	57.6	0.0	0.0	8.786814	2065	3585	1680-1680	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3586	4320	867-867	1.000000	58.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	22	20	40.9	50.0	57.5	58.2	32.7	17.1	2.660914	3586	4320	866-866	1.000000	58.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	22	20	40.9	50.0	57.5	58.4	32.7	9.4	2.892471	3586	4320	882-882	1.000000	56.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	22	20	40.9	50.0	57.5	57.5	32.7	0.0	2.953251	3586	4320	880-880	1.000000	56.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	20	20	40.0	50.0	55.0	58.2	32.7	17.1	3.163567	3586	4320	867-867	1.000000	54.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4358	5173	998-998	1.000000	76.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4358	5173	997-997	1.000000	76.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4358	5173	937-937	1.000000	76.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4358	5173	936-936	1.000000	76.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4358	5173	1022-1022	1.000000	75.6
rps16_p1	rps16	1	AGAGCAAGAAAAGAAGGAAA	TCTCTGTACCGACTGAACCA	20	20	35.0	50.0	52.6	57.7	0.0	0.0	5.691513	5174	6270	1296-1297	1.000000	40.0
rps16_p2	rps16	2	AGAGCAAGAAAAGAAGGAAAT	TCTCTGTACCGACTGAACCA	21	20	33.3	50.0	53.3	57.7	0.0	0.0	5.984059	5174	6270	1296-1297	1.000000	40.0
rps16_p3	rps16	3	AGAGCAAGAAAAGAAGGAAA	GTCTCTGTACCGACTGAACC	20	20	35.0	55.0	52.6	57.4	0.0	0.0	6.043173	5174	6270	1297-1298	1.000000	40.0
rps16_p4	rps16	4	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	56.1	57.7	0.0	0.0	6.184942	5174	6270	1237-1238	1.000000	40.0
rps16_p5	rps16	5	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	20	20	40.0	50.0	52.0	57.7	0.0	0.0	6.245418	5174	6270	1237-1238	1.000000	40.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6271	7238	1041-1041	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6271	7238	1056-1056	1.000000	76.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6271	7238	1055-1055	1.000000	76.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6271	7238	1112-1112	1.000000	75.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6271	7238	1045-1045	1.000000	73.5
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8596	9352	908-908	1.000000	78.1
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8596	9352	890-890	1.000000	75.9
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8596	9352	844-844	1.000000	74.3
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8596	9352	909-909	1.000000	73.9
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8596	9352	826-826	1.000000	72.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9424	10257	890-890	1.000000	78.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9424	10257	908-908	1.000000	75.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9424	10257	889-889	1.000000	72.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9424	10257	898-898	1.000000	72.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9424	10257	947-947	1.000000	71.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATGAACAGCGCAGTAGCAAT	TCTGATTCCCCTGGGATTGT	20	20	45.0	50.0	58.3	58.0	11.5	16.2	0.271617	10964	11603	770-770	1.000000	77.8
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TGAACAGCGCAGTAGCAATA	TCTGATTCCCCTGGGATTGT	20	20	45.0	50.0	57.6	58.0	11.5	16.2	0.392823	10964	11603	769-769	1.000000	76.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAATGAACAGCGCAGTAGCA	TCTGATTCCCCTGGGATTGT	20	20	45.0	50.0	58.5	58.0	11.5	16.2	0.491710	10964	11603	772-772	1.000000	76.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AATGAACAGCGCAGTAGCAA	TCTGATTCCCCTGGGATTGT	20	20	45.0	50.0	58.5	58.0	11.5	16.2	0.491710	10964	11603	771-771	1.000000	76.1
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ATGAACAGCGCAGTAGCAAT	TTCAATAGGCAAGAGCACCG	20	20	45.0	50.0	58.3	58.3	11.5	0.0	0.519401	10964	11603	748-748	1.000000	75.8
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GGGATTTGAACCCTCGTTGT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.308485	12001	12846	990-990	1.000000	61.5
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	TTTGAACCCTCGTTGTCTCC	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	57.8	56.8	0.0	0.0	2.463966	12001	12846	986-986	1.000000	60.3
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	GGGATTTGAACCCTCGTTGT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.1	57.6	0.0	0.0	2.481389	12001	12846	980-980	1.000000	60.1
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	GGGATTTGAACCCTCGTTGT	TCTTCTGTCAAACCCTGATCA	20	21	50.0	42.9	58.1	56.6	0.0	0.0	2.519968	12001	12846	987-987	1.000000	59.8
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGGATTTGAACCCTCGTTGT	GTCAAACCCTGATCAATGAACC	20	22	50.0	45.5	58.1	57.6	0.0	0.0	2.535202	12001	12846	981-981	1.000000	59.7