primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 ATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 19 20 52.6 55.0 56.9 57.9 0.0 13.4 2.157752 1634 2021 445-454 1.000000 68.0 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 ATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 19 20 52.6 55.0 56.9 57.9 0.0 0.0 2.166583 1634 2021 448-457 1.000000 67.9 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 ATCCACTTGGCTACATCCG TAGACCTAGCTGCTGTCGAA 19 20 52.6 50.0 56.9 57.9 0.0 13.4 2.216131 1634 2021 441-450 1.000000 67.6 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ATCCACTTGGCTACATCCGC CCTCTAGACCTAGCTGCTGT 20 20 55.0 55.0 60.2 57.9 0.0 13.4 2.244944 1634 2021 445-454 1.000000 67.3 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 CCACTTGGCTACATCCGC CCTCTAGACCTAGCTGCTGT 18 20 61.1 55.0 58.2 57.9 0.0 13.4 2.252302 1634 2021 443-452 1.000000 67.3 matK_p1 matK 1 CCACTACACTACTGCATGAGC CTTGTTCTGACCGTATCGCA 21 20 52.4 50.0 58.5 58.0 0.0 0.0 1.464516 3678 5201 1709-1724 0.947368 68.6 matK_p2 matK 2 TCAACCACTACACTACTGCA CTTGTTCTGACCGTATCGCA 20 20 45.0 50.0 56.1 58.0 0.0 0.0 1.884608 3678 5201 1713-1728 1.000000 67.4 matK_p3 matK 3 CCACTACACTACTGCATGAGC TTGTTCTGACCGTATCGCAC 21 20 52.4 50.0 58.5 58.3 0.0 0.0 1.750059 3678 5201 1708-1723 0.947368 66.3 matK_p4 matK 4 ACCACTACACTACTGCATGAG CTTGTTCTGACCGTATCGCA 21 20 47.6 50.0 57.1 58.0 0.0 0.0 1.885475 3678 5201 1710-1725 0.947368 65.2 matK_p5 matK 5 CACTACACTACTGCATGAGC CTTGTTCTGACCGTATCGCA 20 20 50.0 50.0 56.0 58.0 0.0 0.0 2.036123 3678 5201 1708-1723 0.947368 64.0 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 GCAAGAATCCACAGCCGATA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.122542 7858 8854 1055-1116 1.000000 85.1 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 7858 8854 1026-1087 1.000000 84.4 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 GCAAGAATCCACAGCCGATA CTCGGAGGTTCGAATCCTTC 20 20 50.0 55.0 58.0 57.8 0.0 16.9 0.257794 7858 8854 1059-1120 1.000000 84.0 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 20 20 45.0 55.0 58.1 57.8 8.1 16.9 0.350677 7858 8854 1030-1091 1.000000 83.3 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 CCACAGCCGATAATGTCCTT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.7 58.1 0.0 16.9 0.408164 7858 8854 1047-1108 1.000000 82.8 atpH-atpI_p1 atpH-atpI 1 AACGGAAGCAGCAGAAATCA TTTTGCAACTTTAGCCGCAG 20 20 45.0 45.0 58.1 57.9 0.0 0.0 0.236520 15735 16653 814-1022 0.947368 82.3 atpH-atpI_p2 atpH-atpI 2 TACCTTGACCAACTCCAGGT TTTTGCAACTTTAGCCGCAG 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.245019 15735 16653 872-1080 0.947368 82.1 atpH-atpI_p3 atpH-atpI 3 AACGGAAGCAGCAGAAATCA CGAATCCATGGAGGGTCATC 20 20 45.0 55.0 58.1 57.8 0.0 21.3 0.279658 15735 16653 784-992 0.947368 82.0 atpH-atpI_p4 atpH-atpI 4 AGCCAATCCAGCAGCAATAA TTTTGCAACTTTAGCCGCAG 20 20 45.0 45.0 57.9 57.9 0.0 0.0 0.281186 15735 16653 832-1040 0.947368 81.9 atpH-atpI_p5 atpH-atpI 5 TACCTTGACCAACTCCAGGT CGAATCCATGGAGGGTCATC 20 20 50.0 55.0 57.9 57.8 0.0 21.3 0.288157 15735 16653 842-1050 0.947368 81.8 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 TCGTCAAACCTTGGTCAATGA CCGGATTTGAACTGGGGAAA 21 20 42.9 50.0 57.8 58.1 0.0 0.0 1.283481 28819 29989 1289-1306 1.000000 72.9 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 TCTTTCGTCAAACCTTGGTCA CCGGATTTGAACTGGGGAAA 21 20 42.9 50.0 57.7 58.1 0.0 0.0 1.351666 28819 29989 1293-1310 1.000000 72.4 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 TCGTCAAACCTTGGTCAATGA TTGTTAATCAGGCGACACCC 21 20 42.9 50.0 57.8 58.2 0.0 0.0 1.385279 28819 29989 1307-1324 1.000000 72.1 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 TCTTTCGTCAAACCTTGGTCA TTGTTAATCAGGCGACACCC 21 20 42.9 50.0 57.7 58.2 0.0 0.0 1.453464 28819 29989 1311-1328 1.000000 71.5 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 TCGTCAAACCTTGGTCAATGA AAAAGGATTTGCAGTCCCCC 21 20 42.9 50.0 57.8 58.4 0.0 14.6 1.556844 28819 29989 1271-1288 1.000000 70.8 trnC-GCA-petN_p1 trnC-GCA-petN 1 TTTTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 20 20 45.0 50.0 57.9 58.2 0.0 0.0 0.204630 30071 31154 1107-1206 1.000000 85.5 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 58.1 58.2 0.0 0.0 0.233476 30071 31154 1051-1150 1.000000 85.3 trnC-GCA-petN_p3 trnC-GCA-petN 3 GGGTGTCGCCTGATTAACAA AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 58.2 58.2 1.7 0.0 0.335274 30071 31154 1033-1132 1.000000 84.5 trnC-GCA-petN_p4 trnC-GCA-petN 4 TTTTGTATCGTTTTGGCGGC AGAGTCCACTTCTTCCCCAT 20 20 45.0 50.0 57.9 57.6 0.0 0.0 0.404145 30071 31154 1168-1267 1.000000 83.7 trnC-GCA-petN_p5 trnC-GCA-petN 5 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 20 20 50.0 50.0 58.1 57.6 0.0 0.0 0.432990 30071 31154 1112-1211 1.000000 83.5 accD-psaI_p1 accD-psaI 1 AGCTACACGGTTTCTTTCCC TTGCAGGAAACACTAAGCCC 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.564273 63357 64028 758-807 1.000000 83.0 accD-psaI_p2 accD-psaI 2 GCTACACGGTTTCTTTCCCT TTGCAGGAAACACTAAGCCC 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.564273 63357 64028 757-806 1.000000 83.0 accD-psaI_p3 accD-psaI 3 AGCTACACGGTTTCTTTCCC CAGGAAACACTAAGCCCACT 20 20 50.0 50.0 57.8 57.4 0.0 0.0 0.749055 63357 64028 755-804 1.000000 81.5 accD-psaI_p4 accD-psaI 4 GCTACACGGTTTCTTTCCCT CAGGAAACACTAAGCCCACT 20 20 50.0 50.0 57.8 57.4 0.0 0.0 0.749055 63357 64028 754-803 1.000000 81.5 accD-psaI_p5 accD-psaI 5 ATTCAAGTAGAGCGCTAGGC TTGCAGGAAACACTAAGCCC 20 20 50.0 50.0 57.5 58.4 11.5 0.0 0.901492 63357 64028 728-777 1.000000 80.4 petA-psbJ_p1 petA-psbJ 1 ACGTGTCCAAGGTTTGTTGT ATTCCGGATTGGGATCATCC 20 20 45.0 50.0 58.2 57.1 0.0 4.1 1.058172 67925 69084 1228-1298 1.000000 78.2 petA-psbJ_p2 petA-psbJ 2 ACGTGTCCAAGGTTTGTTGT ACTGGTATTCCTGTGATCGG 20 20 45.0 50.0 58.2 56.7 0.0 0.0 1.450681 67925 69084 1277-1347 1.000000 75.0 petA-psbJ_p3 petA-psbJ 3 ACGTGTCCAAGGTTTGTTGT CTGGTATTCCTGTGATCGGT 20 20 45.0 50.0 58.2 56.7 0.0 0.0 1.450681 67925 69084 1276-1346 1.000000 75.0 petA-psbJ_p4 petA-psbJ 4 ACGTGTCCAAGGTTTGTTGT GGTTCATATTCCGGATTGGGA 20 21 45.0 47.6 58.2 57.5 0.0 4.1 1.673144 67925 69084 1235-1305 1.000000 73.3 petA-psbJ_p5 petA-psbJ 5 ACGTGTCCAAGGTTTGTTGT ACTGGTATTCCTGTGATCGGT 20 21 45.0 47.6 58.2 58.5 0.0 0.0 1.703141 67925 69084 1277-1347 1.000000 73.0 clpP_p1 clpP 1 AGATCGATCCTAACCAGATGA ACGTTTCCACATCAAAGTGA 21 20 42.9 40.0 55.4 55.5 21.7 0.0 6.100365 75126 77190 2186-2242 1.000000 44.0 clpP_p2 clpP 2 AGATCGATCCTAACCAGATGA ACGTTTCCACATCAAAGTGAA 21 21 42.9 38.1 55.4 56.3 21.7 0.0 6.311519 75126 77190 2186-2242 1.000000 44.0 clpP_p3 clpP 3 AGATCGATCCTAACCAGATGAT ACGTTTCCACATCAAAGTGA 22 20 40.9 40.0 55.9 55.5 21.7 0.0 6.539609 75126 77190 2186-2242 1.000000 44.0 clpP_p4 clpP 4 AGATCGATCCTAACCAGATGA ACGTTTCCACATCAAAGTGAAA 21 22 42.9 36.4 55.4 57.0 21.7 0.0 6.593320 75126 77190 2186-2242 1.000000 44.0 clpP_p5 clpP 5 GATCGATCCTAACCAGATGA ACGTTTCCACATCAAAGTGA 20 20 45.0 40.0 53.8 55.5 14.8 0.0 6.720904 75126 77190 2185-2241 1.000000 44.0 rps8-rpl14_p1 rps8-rpl14 1 GGCAATAGTGTCCCTACCCA GCTCGGGAATTGAGACAGTT 20 20 55.0 50.0 58.8 57.9 0.0 0.0 0.890983 85670 85866 272-285 1.000000 80.9 rps8-rpl14_p2 rps8-rpl14 2 TCGGCAATAGTGTCCCTACC GCTCGGGAATTGAGACAGTT 20 20 55.0 50.0 58.9 57.9 0.0 0.0 0.983454 85670 85866 274-287 1.000000 80.1 rps8-rpl14_p3 rps8-rpl14 3 GGCAATAGTGTCCCTACCCA AACTCGAGTTTTTGGAGCGA 20 20 55.0 45.0 58.8 57.8 0.0 24.9 1.024678 85670 85866 294-307 1.000000 79.5 rps8-rpl14_p4 rps8-rpl14 4 TCGGCAATAGTGTCCCTACC AACTCGAGTTTTTGGAGCGA 20 20 55.0 45.0 58.9 57.8 0.0 24.9 1.117149 85670 85866 296-309 1.000000 78.8 rps8-rpl14_p5 rps8-rpl14 5 TGTTATTCGAACCGTTCCCT GCTCGGGAATTGAGACAGTT 20 20 45.0 50.0 56.9 57.9 0.0 0.0 1.235436 85670 85866 332-345 1.000000 77.4 rps19_p1 rps19 1 AATGAACGACGAGCACCTAC GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 0.0 2.3 0.017031 89134 89412 849-913 1.000000 83.7 rps19_p2 rps19 2 CAATGAACGACGAGCACCTA GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 0.0 2.3 0.018402 89134 89412 850-914 1.000000 83.7 rps19_p3 rps19 3 CGATAGTACCCCCATGGACT GAATGGTTAAAGCGCCCAAC 20 20 55.0 50.0 58.0 58.0 15.2 2.3 0.020323 89134 89412 1173-1237 1.000000 83.5 rps19_p4 rps19 4 CAAGTCGGAAACCAAGTGGA GAATGGTTAAAGCGCCCAAC 20 20 50.0 50.0 58.0 58.0 3.8 2.3 0.047407 89134 89412 1402-1466 1.000000 83.2 rps19_p5 rps19 5 TGTACTTGTGTGCTTGTGCT GAATGGTTAAAGCGCCCAAC 20 20 45.0 50.0 58.0 58.0 0.0 2.3 0.046158 89134 89412 956-956 0.052632 41.7 ndhF_p1 ndhF 1 AGGAAATCCGCAACTGGTTT GGTCTGGTGAATCGGAAACA 20 20 45.0 50.0 58.0 57.8 0.0 0.0 0.183536 114950 117178 2398-2631 0.947368 83.9 ndhF_p2 ndhF 2 GCCCTCCATCAGTGAATCAG GGTCTGGTGAATCGGAAACA 20 20 55.0 50.0 58.0 57.8 0.0 0.0 0.214360 114950 117178 2420-2653 0.947368 83.6 ndhF_p3 ndhF 3 TTGGTCATTTCTTGCCCTCC GGTCTGGTGAATCGGAAACA 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.255556 114950 117178 2433-2666 0.947368 83.3 ndhF_p4 ndhF 4 TGTGCTTCCTGCTGATTACC GGTCTGGTGAATCGGAAACA 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.292772 114950 117178 2507-2740 0.947368 82.9 ndhF_p5 ndhF 5 GGATCGTGGGATTCGTTCAA GGTCTGGTGAATCGGAAACA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.197454 114950 117178 2346-2383 0.894737 78.5 ndhF-rpl32_p1 ndhF-rpl32 1 CTGGAAGTGGAGCAAAAGGT TTCGACACAAGAGGGAGTCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.016319 117179 118374 1968-2026 1.000000 87.8 ndhF-rpl32_p2 ndhF-rpl32 2 CTGGAAGTGGAGCAAAAGGT CCATTCGACACAAGAGGGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.028853 117179 118374 1971-2029 1.000000 87.8 ndhF-rpl32_p3 ndhF-rpl32 3 CGGAACAAACAGAAGTCCCA TTCGACACAAGAGGGAGTCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.041408 117179 118374 2006-2064 1.000000 87.6 ndhF-rpl32_p4 ndhF-rpl32 4 CGGAACAAACAGAAGTCCCA CCATTCGACACAAGAGGGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.053942 117179 118374 2009-2067 1.000000 87.5 ndhF-rpl32_p5 ndhF-rpl32 5 AGGGGAAATTGTGCGGATTT TTCGACACAAGAGGGAGTCT 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.066011 117179 118374 2653-2711 1.000000 86.1 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 ACCGATTCAATTTCCACGGT TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 57.8 57.9 0.0 0.0 0.318479 118531 119895 1886-1943 0.947368 83.3 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 ACCGATTCAATTTCCACGGT ACTGGGATTGTAGAGCAGGT 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.235913 118531 119895 2435-2493 0.894737 81.2 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAAGGAAAGGGATGTCAGGC TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.346504 118531 119895 1462-1516 0.842105 78.4 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 ACTCGATCTCGGAGCTTGAT ACTGGGATTGTAGAGCAGGT 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.355357 118531 119895 2607-2666 0.842105 78.0 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AAAGGAAAGGGATGTCAGGC ACTGGGATTGTAGAGCAGGT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.263939 118531 119895 2011-2066 0.789474 76.3 ndhD_p1 ndhD 1 TCACAGGATTCTTTGACTTTATGTC GCGGGTTTTTCTGGTCAAAG 25 20 36.0 50.0 57.3 57.9 0.0 0.0 5.807184 121256 122761 1589-1589 1.000000 41.9 ndhD_p2 ndhD 2 CACAGGATTCTTTGACTTTATGTCT GCGGGTTTTTCTGGTCAAAG 25 20 36.0 50.0 57.3 57.9 0.2 0.0 5.813149 121256 122761 1588-1588 1.000000 41.9 ndhD_p3 ndhD 3 TCACAGGATTCTTTGACTTTATGTC ACCGACAAAAACCCGTACTC 25 20 36.0 50.0 57.3 58.1 0.0 0.0 5.803209 121256 122761 1626-1627 1.000000 41.8 ndhD_p4 ndhD 4 CACAGGATTCTTTGACTTTATGTCT ACCGACAAAAACCCGTACTC 25 20 36.0 50.0 57.3 58.1 0.2 0.0 5.809174 121256 122761 1625-1626 1.000000 41.8 ndhD_p5 ndhD 5 TCACAGGATTCTTTGACTTTATGT ACCGACAAAAACCCGTACTC 24 20 33.3 50.0 56.3 58.1 0.0 0.0 5.834460 121256 122761 1626-1627 1.000000 41.8 ndhG-ndhI_p1 ndhG-ndhI 1 CACCCCCAGAGCTCCTAATA ATCAAATTGCTTTGGGTCGA 20 20 55.0 40.0 57.9 55.9 10.9 0.0 2.181874 124484 124880 542-550 0.947368 66.0 ndhG-ndhI_p2 ndhG-ndhI 2 CACCCCCAGAGCTCCTAATA TCAAATTGCTTTGGGTCGAT 20 20 55.0 40.0 57.9 55.9 10.9 0.0 2.181874 124484 124880 541-549 0.947368 66.0 ndhG-ndhI_p3 ndhG-ndhI 3 CACCCCCAGAGCTCCTAATA TCAAATTGCTTTGGGTCGATT 20 21 55.0 38.1 57.9 56.7 10.9 0.0 2.407141 124484 124880 541-549 0.947368 64.2 ndhG-ndhI_p4 ndhG-ndhI 4 TAATACCACCCCCAGAGCTC ATCAAATTGCTTTGGGTCGA 20 20 55.0 40.0 58.2 55.9 5.3 0.0 2.276167 124484 124880 552-552 0.052632 23.9 ndhG-ndhI_p5 ndhG-ndhI 5 TAATACCACCCCCAGAGCTC TCAAATTGCTTTGGGTCGAT 20 20 55.0 40.0 58.2 55.9 5.3 0.0 2.276167 124484 124880 551-551 0.052632 23.9 rps15-ycf1_p1 rps15-ycf1 1 TGAAATTCAACAGACCCTTTGT TGGCAGTCGTTTCAGTATGT 22 20 36.4 45.0 56.4 57.2 0.0 0.0 4.458469 129174 129549 502-516 0.947368 49.5 rps15-ycf1_p2 rps15-ycf1 2 TGAAATTCAACAGACCCTTTGTT TGGCAGTCGTTTCAGTATGT 23 20 34.8 45.0 57.1 57.2 0.0 0.0 4.762522 129174 129549 502-516 0.947368 47.0 rps15-ycf1_p3 rps15-ycf1 3 TTGAAATTCAACAGACCCTTTGT TGGCAGTCGTTTCAGTATGT 23 20 34.8 45.0 57.1 57.2 4.0 0.0 4.762522 129174 129549 503-517 0.947368 47.0 rps15-ycf1_p4 rps15-ycf1 4 ACTTGAAATTCAACAGACCCT TGGCAGTCGTTTCAGTATGT 21 20 38.1 45.0 55.3 57.2 4.0 0.0 4.516440 129174 129549 505-519 0.894737 45.9 rps15-ycf1_p5 rps15-ycf1 5 ACTTGAAATTCAACAGACCCTT TGGCAGTCGTTTCAGTATGT 22 20 36.4 45.0 56.1 57.2 4.0 0.0 4.737958 129174 129549 505-519 0.894737 44.2 ycf1_p1 ycf1 1 TGAGAAGAACCAAGATTCCAGG AAAATTACTAAGACTGGGACTAA 22 23 45.5 30.4 57.7 52.8 0.0 0.0 10.508127 130634 130753 180-204 1.000000 52.0 ycf1_p2 ycf1 2 GAGAAGAACCAAGATTCCAGGT AAAATTACTAAGACTGGGACTAA 22 23 45.5 30.4 57.7 52.8 0.0 0.0 10.509603 130634 130753 179-203 1.000000 52.0 ycf1_p3 ycf1 3 GGCACAAATTCTGAGAAGAACC AAAATTACTAAGACTGGGACTAA 22 23 45.5 30.4 57.8 52.8 0.0 0.0 10.382128 130634 130753 191-215 1.000000 51.9 ycf1_p4 ycf1 4 TGCATCATAAAGTGGCACAA AAAATTACTAAGACTGGGACTAA 20 23 40.0 30.4 55.9 52.8 21.4 0.0 10.291895 130634 130753 204-228 1.000000 51.8 ycf1_p5 ycf1 5 AGTGGCACAAATTCTGAGAAGA AAAATTACTAAGACTGGGACTAA 22 23 40.9 30.4 57.9 52.8 21.4 0.0 10.306702 130634 130753 194-218 1.000000 51.8