primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109256	16750	17901	1341-1874	0.973451	90.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	TCTTCCACTTGCTTCTGCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138913	16750	17901	1432-1965	0.778761	82.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CGAGCCCATATCCTTGCTTT	TTCAGGCACAACACCATTCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.091561	16750	17901	2000-2492	0.504425	71.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGAGCCCATATCCTTGCTTT	ATTCCATTCGTGACTGTGGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.140988	16750	17901	1786-2211	0.141593	56.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CGAGCCCATATCCTTGCTTT	GCAGCAGCCACTACATTAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056966	16750	17901	1605-2059	0.097345	54.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	17973	18715	503-1027	1.000000	88.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	17973	18715	442-966	0.955752	88.4
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	17973	18715	521-1045	1.000000	86.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	17973	18715	460-984	0.955752	86.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	17973	18715	441-965	0.955752	82.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGAACTAGAATGAACAGTGT	GTTCGAGCCCCGTCAGTC	21	18	38.1	66.7	54.2	60.1	0.0	0.0	8.923004	19880	20784	759-1007	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GCAGTAGGAACTAGAATGAACAGT	GTTCGAGCCCCGTCAGTC	24	18	41.7	66.7	58.3	60.1	0.0	0.0	8.400669	19880	20784	764-1012	0.982301	51.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GCAGTAGGAACTAGAATGAACAG	GTTCGAGCCCCGTCAGTC	23	18	43.5	66.7	56.7	60.1	0.0	0.0	8.419334	19880	20784	764-1012	0.982301	51.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GCAGTAGGAACTAGAATGAACA	GTTCGAGCCCCGTCAGTC	22	18	40.9	66.7	55.6	60.1	0.0	0.0	8.554576	19880	20784	764-1012	0.982301	51.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGTAGTAGCA	GTTCGAGCCCCGTCAGTC	21	18	38.1	66.7	54.3	60.1	0.0	0.0	8.802946	19880	20784	752-1000	0.964602	50.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	ATTCTTCTTGGGTCGATGCC	20	20	50.0	50.0	58.5	58.0	7.1	0.0	0.521454	20862	21340	387-635	0.982301	87.1
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	20862	21340	334-582	0.982301	85.6
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	20862	21340	333-581	0.982301	85.5
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	20862	21340	355-603	0.973451	84.3
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	20862	21340	354-602	0.973451	84.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	21562	22752	677-1348	0.991150	90.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	21562	22752	639-1310	0.991150	89.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	21562	22752	618-1289	0.991150	89.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	21562	22752	656-1327	0.946903	88.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	21562	22752	653-1324	0.946903	87.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	20	20	55.0	55.0	58.1	58.5	7.6	0.0	0.582197	22825	24371	1373-1762	0.982301	86.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.604351	22825	24371	1376-1765	0.973451	86.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TCCCTCCGTAACCAGTCATC	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.800935	22825	24371	1350-1739	1.000000	85.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	GTCCCTCCGTAACCAGTCAT	20	20	55.0	55.0	58.1	58.8	7.6	0.0	0.871639	22825	24371	1374-1763	0.982301	84.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CCTCCGTAACCAGTCATCCA	20	20	55.0	55.0	58.1	58.8	7.6	0.0	0.872532	22825	24371	1371-1760	0.982301	84.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	TACCATTCAACTATATCCGC	20	20	55.0	40.0	58.0	52.3	0.0	0.0	5.738913	27641	28223	470-754	0.964602	50.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTTCTCCTGATGGTTGGTCG	TTACCATTCAACTATATCCGC	20	21	55.0	38.1	58.0	53.2	0.0	0.0	5.796361	27641	28223	471-755	0.964602	50.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	TACCATTCAACTATATCCGC	20	20	50.0	40.0	57.8	52.3	0.0	0.0	5.928199	27641	28223	473-757	0.964602	50.6
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CTTCTCCTGATGGTTGGTCG	TTTACCATTCAACTATATCCGC	20	22	55.0	36.4	58.0	54.1	0.0	0.0	5.938371	27641	28223	472-756	0.964602	50.6
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GATGGTTGGTCGGGTAACAA	TACCATTCAACTATATCCGC	20	20	50.0	40.0	57.8	52.3	0.0	0.0	5.911239	27641	28223	462-746	0.902655	48.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TTGACCCGTTGATCCTTTGG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.100337	38344	39607	1280-2018	0.964602	89.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TACCCTTCCGGTTCTGGATT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043491	38344	39607	956-1606	0.823009	84.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TACCCTTCCGGTTCTGGATT	ACGCAGTCAACTCCATTTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.071549	38344	39607	1177-1825	0.778761	82.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CCGGTTCTGGGCTTTTTACT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098986	38344	39607	1139-1831	0.495575	71.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TCCGGTTCTGGGCTTTTTAC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.104378	38344	39607	1140-1832	0.495575	71.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ATCATATTCATAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	23	20	26.1	55.0	52.2	58.1	0.0	0.0	8.877702	43140	43803	587-877	1.000000	52.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCATAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	23	20	26.1	55.0	52.2	58.1	0.0	0.0	8.877702	43140	43803	586-876	1.000000	52.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCAGAAAATATCATATTCATAAAGCA	CCGAGAAAGTCTACGGTTCG	27	20	29.6	55.0	56.4	58.1	0.0	0.0	8.754635	43140	43803	597-887	0.991150	51.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ACCCAGAAAATATCATATTCATAAAGC	CCGAGAAAGTCTACGGTTCG	27	20	29.6	55.0	56.4	58.1	0.0	0.0	8.756505	43140	43803	598-888	0.991150	51.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCAGAAAATATCATATTCATAAAGC	CCGAGAAAGTCTACGGTTCG	26	20	30.8	55.0	54.9	58.1	0.0	0.0	9.216062	43140	43803	597-887	0.991150	51.6
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	TTGAACCCACAAATGCCTGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.242901	48986	49693	1310-1502	0.911504	86.5
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	ATAGGAGCCCAAGTACCTGG	20	20	50.0	55.0	58.0	58.2	0.0	3.3	0.222024	48986	49693	1467-1642	0.256637	60.5
rbcL-accD_p3	rbcL-accD	3	CGCTGGTACCGTAGTAGGTA	ATAGGAGCCCAAGTACCTGG	20	20	55.0	55.0	58.0	58.2	7.6	3.3	0.238627	48986	49693	1780-1955	0.256637	60.4
rbcL-accD_p4	rbcL-accD	4	ACTACAATTCGGTGGAGGGA	ATAGGAGCCCAAGTACCTGG	20	20	50.0	55.0	58.1	58.2	0.0	3.3	0.245202	48986	49693	1564-1739	0.256637	60.3
rbcL-accD_p5	rbcL-accD	5	GGGTCTCTCTACCAGGTGTT	ATAGGAGCCCAAGTACCTGG	20	20	55.0	55.0	58.1	58.2	0.8	3.3	0.252206	48986	49693	1658-1833	0.256637	60.2
psbE-petL_p1	psbE-petL	1	AAAGGATCAAAACGGCCAGT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.041452	56543	57832	1380-2096	1.000000	91.7
psbE-petL_p2	psbE-petL	2	AAGGATCAAAACGGCCAGTT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.041452	56543	57832	1379-2095	1.000000	91.7
psbE-petL_p3	psbE-petL	3	ACGAGGGCTTCCAAAAACAT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041452	56543	57832	1315-2031	1.000000	91.7
psbE-petL_p4	psbE-petL	4	AGGGCTTCCAAAAACATCGT	AGAACGTGGGTCTCCAAAAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041452	56543	57832	1312-2028	0.991150	91.3
psbE-petL_p5	psbE-petL	5	AGGGCTTCCAAAAACATCGT	CCTACAGAGCGTGATTGCAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.059236	56543	57832	1272-1988	0.929204	88.7
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019669	64058	64536	600-674	0.955752	90.1
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	64058	64536	553-627	0.982301	89.4
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CAAACGACCGGGATCATTCA	20	20	50.0	50.0	58.0	58.3	0.0	6.3	0.268011	64058	64536	590-664	0.982301	89.1
clpP-psbB_p4	clpP-psbB	4	CGTCAACCCAAGATGCATCT	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.3	58.0	23.8	0.0	0.275956	64058	64536	591-665	0.973451	88.7
clpP-psbB_p5	clpP-psbB	5	TCCTCTCCAGGACTTCGAAA	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.322939	64058	64536	571-645	0.964602	88.0
ycf1-ndhF_p1	ycf1-ndhF	1	ACAGGAACAAGAGGGATCCA	GGTTACCTGATGCCATGGAG	20	20	50.0	55.0	58.0	58.0	15.2	18.4	0.071921	114088	115114	1873-2128	0.982301	90.7
ycf1-ndhF_p2	ycf1-ndhF	2	ACAGGAACAAGAGGGATCCA	GTTACCTGATGCCATGGAGG	20	20	50.0	55.0	58.0	58.0	15.2	18.4	0.071921	114088	115114	1872-2127	0.982301	90.7
ycf1-ndhF_p3	ycf1-ndhF	3	AAGAGGGATCCACCGAAGAA	GTTACCTGATGCCATGGAGG	20	20	50.0	55.0	58.0	58.0	15.2	18.4	0.074506	114088	115114	1864-2119	0.982301	90.7
ycf1-ndhF_p4	ycf1-ndhF	4	ACAGGAACAAGAGGGATCCA	ACCTATTGCGTCGAATGCTT	20	20	50.0	45.0	58.0	58.0	15.2	11.7	0.068968	114088	115114	2115-2370	0.955752	89.7
ycf1-ndhF_p5	ycf1-ndhF	5	AAGAGGGATCCACCGAAGAA	ACCTATTGCGTCGAATGCTT	20	20	50.0	45.0	58.0	58.0	15.2	11.7	0.071554	114088	115114	2107-2362	0.955752	89.7
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCATTCGACGCAATAGGT	TGCCCAATACCCTTTGCTTT	20	20	45.0	45.0	58.0	58.0	8.6	0.0	0.046893	116315	117295	988-1626	0.769912	82.4
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCATTCGACGCAATAGGT	TGTTTAGGGTCGATGGGGAT	20	20	45.0	50.0	58.0	58.1	8.6	0.0	0.136378	116315	117295	1401-2051	0.752212	81.0
ndhF-rpl32_p3	ndhF-rpl32	3	ACCACACATGAAGCGGAAAT	TGCCCAATACCCTTTGCTTT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.113018	116315	117295	1213-1851	0.725664	80.1
ndhF-rpl32_p4	ndhF-rpl32	4	TTTTGACAAGCATTCGACGC	TGTTTAGGGTCGATGGGGAT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.143240	116315	117295	1408-2058	0.725664	79.9
ndhF-rpl32_p5	ndhF-rpl32	5	TTTTGACAAGCATTCGACGC	TGCCCAATACCCTTTGCTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.053754	116315	117295	995-1633	0.646018	77.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAAGCAAAGGGTATTGGGCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030548	117455	118574	761-1311	0.884956	87.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAGCAAAGGGTATTGGGCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.031919	117455	118574	762-1312	0.884956	87.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAAGCAAAGGGTATTGGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.146995	117455	118574	771-1321	0.884956	86.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGAAAATAGACAATGCC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.113153	117455	118574	1100-1661	0.787611	82.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGGCGAAAATAGACAATGCC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.114524	117455	118574	1101-1662	0.787611	82.6
ycf1_p1	ycf1	1	GGTGCCAAGGTTTAAGACGA	ATCCTCCGGATGGGAATCAA	20	20	50.0	50.0	58.1	58.2	0.0	15.9	0.293361	131982	132101	1482-1551	0.964602	88.2
ycf1_p2	ycf1	2	TCGGTGCCAAGGTTTAAGAC	ATCCTCCGGATGGGAATCAA	20	20	50.0	50.0	58.1	58.2	0.0	15.9	0.293361	131982	132101	1484-1553	0.929204	86.8
ycf1_p3	ycf1	3	TCTTTGAGGGCGCATAGAAC	ATCCTCCGGATGGGAATCAA	20	20	50.0	50.0	58.0	58.2	10.8	15.9	0.204423	131982	132101	920-989	0.893805	86.1
ycf1_p4	ycf1	4	GTCTTTGAGGGCGCATAGAA	ATCCTCCGGATGGGAATCAA	20	20	50.0	50.0	58.0	58.2	10.8	15.9	0.204423	131982	132101	921-990	0.884956	85.8
ycf1_p5	ycf1	5	ATCCTCGGACCATTCAGGAA	ATCCTCCGGATGGGAATCAA	20	20	50.0	50.0	58.1	58.2	3.3	15.9	0.300673	131982	132101	1388-1400	0.115044	50.9