primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	87	456	427-439	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CGGTCGAGGCTCCATCTATA	20	20	55.0	55.0	58.1	58.2	0.0	9.3	0.306414	87	456	408-420	1.000000	89.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	87	456	476-488	1.000000	88.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.525510	87	456	477-489	1.000000	87.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GCCTTGATCCACTTGGCTAC	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.6	57.9	0.0	0.0	0.691206	87	456	432-444	1.000000	86.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGATGTCGAGTCAAGAGC	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	58.0	58.0	9.7	0.0	0.065556	6259	6958	1468-1583	1.000000	91.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGATGTCGAGTCAAGAGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	9.7	16.9	0.092129	6259	6958	802-877	1.000000	91.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGATGTCGAGTCAAGAGC	ATCGAAAACTTACAGCGGCT	20	20	50.0	45.0	58.0	57.9	9.7	0.0	0.110790	6259	6958	1470-1585	1.000000	91.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AACGATGTCGAGTCAAGAGC	TCGAAAACTTACAGCGGCTT	20	20	50.0	45.0	58.0	58.1	9.7	0.0	0.142330	6259	6958	1469-1584	1.000000	90.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTTTTCTCCTCGTACGGC	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	57.9	58.0	16.5	0.0	0.125507	6259	6958	2080-2193	1.000000	90.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	TCCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	19	20	57.9	45.0	58.1	54.0	0.0	0.0	5.103373	8455	9062	634-811	1.000000	52.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	TCCACTCAGCCATCTCTCC	TTACCACTAAACTATACCCGC	19	21	57.9	42.9	58.1	54.9	0.0	0.0	5.239633	8455	9062	635-812	1.000000	52.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TCCACTCAGCCATCTCTCC	TTTACCACTAAACTATACCCGC	19	22	57.9	40.9	58.1	55.6	0.0	0.0	5.453521	8455	9062	636-813	1.000000	52.0
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TCCACTCAGCCATCTCTCC	CTTTTACCACTAAACTATACCCGC	19	24	57.9	41.7	58.1	57.4	0.0	0.0	5.686581	8455	9062	638-815	1.000000	52.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TCCACTCAGCCATCTCTCC	TTTTACCACTAAACTATACCCGC	19	23	57.9	39.1	58.1	56.4	0.0	0.0	5.735021	8455	9062	637-814	1.000000	52.0
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	CCCGCTACCCGCTTATATTC	TGAGTTGCTCAAACAGTCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.038652	10137	10530	992-1036	1.000000	91.4
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CCCGCTACCCGCTTATATTC	GAGTTGCTCAAACAGTCCCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.038652	10137	10530	991-1035	1.000000	91.3
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGCCTTCCAAGCTAACGATG	TGAGTTGCTCAAACAGTCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062520	10137	10530	1023-1067	1.000000	91.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AGCCTTCCAAGCTAACGATG	GAGTTGCTCAAACAGTCCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062520	10137	10530	1022-1066	1.000000	90.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CCCGCTACCCGCTTATATTC	CGAAGCTTATCCGGGAGATG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.077659	10137	10530	1394-1438	1.000000	89.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CAACCACTCAGCCATCTCTC	CTTGAGGTCACGGGTTCAAA	20	20	55.0	50.0	58.0	58.0	0.0	4.2	0.059562	37896	38367	1299-1401	1.000000	90.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CAACCACTCAGCCATCTCTC	CTTGTGTTTATCGCGGGGTA	20	20	55.0	50.0	58.0	58.0	0.0	6.1	0.034292	37896	38367	1350-1408	0.875000	81.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CAACCACTCAGCCATCTCTC	TTGTGTTTATCGCGGGGTAG	20	20	55.0	50.0	58.0	58.0	0.0	6.1	0.034292	37896	38367	1349-1407	0.875000	81.9
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CAACCACTCAGCCATCTCTC	CGATCATAGGAGGGTCCCTT	20	20	55.0	55.0	58.0	58.0	0.0	13.1	0.038260	37896	38367	1458-1516	0.875000	81.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	20	20	55.0	50.0	58.0	58.0	0.0	9.5	0.034641	37896	38367	1622-1680	0.875000	81.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48251	49119	889-1051	1.000000	89.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48251	49119	897-1059	1.000000	89.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48251	49119	921-1083	1.000000	88.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48251	49119	929-1091	1.000000	88.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	48251	49119	903-1065	1.000000	87.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ACTCGATATTATTAGAAATGCCCA	GTTCGAGTCCGTATAGCCCT	24	20	33.3	55.0	55.8	58.7	0.0	0.0	6.881539	52647	53207	519-651	1.000000	50.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTCGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	55.8	57.8	0.0	0.0	6.395473	52647	53207	531-663	1.000000	50.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ACTCGATATTATTAGAAATGCCCAA	AGAAGGTCTACGGTTCGAGT	25	20	32.0	50.0	56.5	57.8	0.0	0.0	6.729726	52647	53207	531-663	1.000000	50.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ACTCGATATTATTAGAAATGCCCA	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	55.8	57.5	0.0	0.0	6.739245	52647	53207	530-662	1.000000	50.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	ACTCGATATTATTAGAAATGCCCA	AAGGTCTACGGTTCGAGTCC	24	20	33.3	55.0	55.8	58.8	0.0	0.0	7.026015	52647	53207	529-661	1.000000	50.8
rbcL-accD_p1	rbcL-accD	1	AGTCCTGAATTAGCTGCTGC	CTCCCTGTTAGACAACGTCG	20	20	50.0	55.0	58.0	58.0	13.4	0.0	0.056988	58491	59316	819-949	0.875000	81.4
rbcL-accD_p2	rbcL-accD	2	ATGAAATTATCCGTGCGGCT	CTCCCTGTTAGACAACGTCG	20	20	45.0	55.0	58.0	58.0	5.6	0.0	0.054140	58491	59316	848-978	0.875000	81.3
rbcL-accD_p3	rbcL-accD	3	ATGAAATTATCCGTGCGGCT	GAAATTCCGAATGTCCCCGA	20	20	45.0	50.0	58.0	58.0	5.6	0.2	0.065362	58491	59316	1025-1155	0.875000	81.1
rbcL-accD_p4	rbcL-accD	4	AGTCCTGAATTAGCTGCTGC	TCTTCGCATTGAACCCACAA	20	20	50.0	45.0	58.0	58.0	13.4	0.0	0.061265	58491	59316	1466-1575	0.875000	80.8
rbcL-accD_p5	rbcL-accD	5	ATGAAATTATCCGTGCGGCT	TCTTCGCATTGAACCCACAA	20	20	45.0	45.0	58.0	58.0	5.6	0.0	0.058416	58491	59316	1495-1604	0.875000	80.7
accD_p1	accD	1	TCTTTTCATGCCGTGTTGGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	0.0	26.9	0.052738	59317	60837	2508-2792	0.875000	81.5
accD_p2	accD	2	TCTTTTCATGCCGTGTTGGA	AAGGAAGCCATTGCAATTGC	20	20	45.0	45.0	58.0	57.9	0.0	24.1	0.129272	59317	60837	2515-2799	0.875000	80.9
accD_p3	accD	3	TCTTTTCATGCCGTGTTGGA	TCTTGCCTAATTTCGCGGAA	20	20	45.0	45.0	58.0	57.9	0.0	0.3	0.127805	59317	60837	2162-2230	0.750000	72.9
accD_p4	accD	4	TCTTTTCATGCCGTGTTGGA	TGCCTAATTTCGCGGAAGAA	20	20	45.0	45.0	58.0	57.9	0.0	5.8	0.127805	59317	60837	2159-2227	0.750000	72.9
accD_p5	accD	5	TCTTTTCATGCCGTGTTGGA	TTGCCTAATTTCGCGGAAGA	20	20	45.0	45.0	58.0	57.9	0.0	5.8	0.127805	59317	60837	2160-2228	0.750000	72.9
clpP_p1	clpP	1	CTGAACGAGTCGCACATACA	TACGCTTCCGCATCAAAGAA	20	20	50.0	45.0	58.0	57.9	13.6	0.0	0.110313	71953	74134	2533-2571	0.875000	80.2
clpP_p2	clpP	2	CTGAACGAGTCGCACATACA	TTACGCTTCCGCATCAAAGA	20	20	50.0	45.0	58.0	57.9	13.6	0.0	0.110313	71953	74134	2534-2572	0.875000	80.2
clpP_p3	clpP	3	TGACCCTGTTAGTCCGTTCT	TACGCTTCCGCATCAAAGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.092871	71953	74134	2863-2901	0.875000	80.0
clpP_p4	clpP	4	TGACCCTGTTAGTCCGTTCT	TTACGCTTCCGCATCAAAGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.092871	71953	74134	2864-2902	0.875000	80.0
clpP_p5	clpP	5	TGACCCTGTTAGTCCGTTCT	CATTGTTACGCTTCCGCATC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.096522	71953	74134	2869-2907	0.875000	79.9
rps3-rps19_p1	rps3-rps19	1	AGTCTCAAAACGAGCGGATC	TAGTCACTTGGTCCAGAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.012012	85816	86383	777-792	0.875000	80.5
rps3-rps19_p2	rps3-rps19	2	GTCTCAAAACGAGCGGATCT	TAGTCACTTGGTCCAGAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.012012	85816	86383	776-791	0.875000	80.5
rps3-rps19_p3	rps3-rps19	3	AGTCTCAAAACGAGCGGATC	AACCCTGTAGACCATCCACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041194	85816	86383	1118-1133	0.875000	79.2
rps3-rps19_p4	rps3-rps19	4	GTCTCAAAACGAGCGGATCT	AACCCTGTAGACCATCCACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041194	85816	86383	1117-1132	0.875000	79.2
rps3-rps19_p5	rps3-rps19	5	GTCTCAAAACGAGCGGATCT	CCGGATCGAAATGTTGGCTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.054227	85816	86383	1170-1185	0.875000	78.9
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	TATGTAGGAGGTGGCCGAAT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.099441	111332	112315	1161-1616	1.000000	91.2
ycf1_p2	ycf1	2	GAGCTACTGAGGAACAACGG	ACTTTCTTCGTTGGCACCAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021074	111332	112315	1620-2084	0.875000	86.8
ycf1_p3	ycf1	3	TAACTCGCGGAACTTCTTCG	ACTTTCTTCGTTGGCACCAT	20	20	50.0	45.0	58.0	58.0	6.1	0.0	0.045605	111332	112315	1871-1994	0.750000	78.0
ycf1_p4	ycf1	4	GTATCCCGGACCTACACTGA	ACTTTCTTCGTTGGCACCAT	20	20	55.0	45.0	57.9	58.0	0.7	0.0	0.081714	111332	112315	2262-2317	0.500000	65.1
ycf1_p5	ycf1	5	AGTATCCCGGACCTACACTG	ACTTTCTTCGTTGGCACCAT	20	20	55.0	45.0	57.9	58.0	0.7	0.0	0.087578	111332	112315	2263-2318	0.500000	65.0
ycf1-ndhF_p1	ycf1-ndhF	1	GATATCCGCCGAAGAAGACC	ACTTTCTTCGTTGGCACCAT	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.086157	112316	112386	804-804	0.250000	49.3
ycf1-ndhF_p2	ycf1-ndhF	2	AGCGGTCAAATTGAGAAGCA	ACTTTCTTCGTTGGCACCAT	20	20	45.0	45.0	58.1	58.0	15.8	0.0	0.122524	112316	112386	836-836	0.250000	49.0
ycf1-ndhF_p3	ycf1-ndhF	3	GATATCCGCCGAAGAAGACC	AATATGTAGGAGGTGGCCGA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.164524	112316	112386	338-338	0.250000	48.7
ycf1-ndhF_p4	ycf1-ndhF	4	GATATCCGCCGAAGAAGACC	ATATGTAGGAGGTGGCCGAA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.164524	112316	112386	337-337	0.250000	48.7
ycf1-ndhF_p5	ycf1-ndhF	5	GATATCCGCCGAAGAAGACC	TATGTAGGAGGTGGCCGAAT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.164524	112316	112386	336-336	0.250000	48.7
ndhF-rpl32_p1	ndhF-rpl32	1	TAGGGCGTGTGAACCAAAAA	TTCCATGCTGCCCAATATCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.054584	114639	115659	1618-1636	0.625000	73.6
ndhF-rpl32_p2	ndhF-rpl32	2	TAGGGCGTGTGAACCAAAAA	CATGCTGCCCAATATCCCTT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.059602	114639	115659	1615-1633	0.625000	73.5
ndhF-rpl32_p3	ndhF-rpl32	3	GAAACTGCCCGTTATCCGAT	TTCCATGCTGCCCAATATCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.050886	114639	115659	1719-1719	0.250000	49.6
ndhF-rpl32_p4	ndhF-rpl32	4	GAAACTGCCCGTTATCCGAT	CATGCTGCCCAATATCCCTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.055905	114639	115659	1716-1716	0.250000	49.6
ndhF-rpl32_p5	ndhF-rpl32	5	GAAACTGCCCGTTATCCGAT	GGTGGAGACCTTGTTGTGTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.103317	114639	115659	1932-1932	0.250000	49.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGGTTGGGTTCGTAAACTT	ACTCGGTGCGATTATTGTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.078594	115831	116188	1335-1360	0.875000	79.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGATATTGGGCAGCATGGAA	ACTCGGTGCGATTATTGTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.064419	115831	116188	973-973	0.250000	49.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGATATTGGGCAGCATG	ACTCGGTGCGATTATTGTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.069438	115831	116188	976-976	0.250000	49.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGATATTGGGCAGCATGGAA	GCTGCATAGCCCAATACCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085211	115831	116188	1104-1104	0.250000	49.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGGATATTGGGCAGCATG	GCTGCATAGCCCAATACCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.090229	115831	116188	1107-1107	0.250000	49.3
ndhG-ndhI_p1	ndhG-ndhI	1	GTGCTGCAGCTACAAAATGG	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	36.6	0.0	0.110982	120760	121167	675-766	0.875000	86.1
ndhG-ndhI_p2	ndhG-ndhI	2	GATTTCTTACTGGCGAGCCA	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	16.7	0.0	0.121855	120760	121167	1028-1119	0.875000	83.2
ndhG-ndhI_p3	ndhG-ndhI	3	GTGCTGCAGCTACAAAATGG	TGCCTAGGCTACGGATAGAC	20	20	50.0	55.0	58.0	58.1	36.6	33.1	0.106312	120760	121167	1277-1356	0.875000	78.7
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCGATCCCAGAAAGA	TGCCTAGGCTACGGATAGAC	20	20	50.0	55.0	58.0	58.1	0.0	33.1	0.140389	120760	121167	1150-1229	0.875000	78.7
ndhG-ndhI_p5	ndhG-ndhI	5	GATTTCTTACTGGCGAGCCA	TGCCTAGGCTACGGATAGAC	20	20	50.0	55.0	58.0	58.1	16.7	33.1	0.117186	120760	121167	1630-1709	0.750000	71.8
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.036329	154120	154426	906-1135	1.000000	91.7
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	AATCATGGTCCTTGCGGATC	ACCGGGAGTCGATGACTATT	20	20	50.0	50.0	58.0	57.9	0.0	6.3	0.095962	154120	154426	1303-1517	1.000000	90.6
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	AATCATGGTCCTTGCGGATC	AGAAGAGTTCGACCCAATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.127733	154120	154426	1260-1474	1.000000	90.6
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	AATCATGGTCCTTGCGGATC	GAAGAGTTCGACCCAATGCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.127733	154120	154426	1259-1473	1.000000	90.6
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	AATCATGGTCCTTGCGGATC	GACTGTCTACGGCTCCATTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.099233	154120	154426	1491-1802	1.000000	90.3