primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTGGACCTAGCTGCTAT	20	20	55.0	55.0	58.1	58.0	0.0	10.2	0.137162	80	369	340-357	1.000000	88.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTGGACCTAGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	10.2	0.141524	80	369	354-371	1.000000	87.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTGGACCTAGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	10.2	0.143031	80	369	353-370	1.000000	87.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTGGACCTAGCTGCTAT	20	20	50.0	55.0	57.9	58.0	0.0	10.2	0.143031	80	369	355-372	1.000000	87.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTTCAGGCTGAGCACAACAT	20	20	55.0	45.0	58.1	58.0	0.0	17.9	0.143447	80	369	802-819	1.000000	83.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGCGGGTTTCTTGGGTTATC	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	57.9	57.9	0.0	18.8	0.200256	4223	4959	1709-1751	1.000000	83.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTCGGAGGCAGGTAAATTC	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	11.8	18.8	0.121052	4223	4959	1648-1673	0.800000	74.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTCGGAGGCAGGTAAATTC	TCTATCCCAATGAGCCGTCT	20	20	55.0	50.0	58.0	57.9	11.8	0.0	0.133177	4223	4959	1593-1618	0.800000	74.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTCGGAGGCAGGTAAATTC	AGCCGTCTATCGAATCGTTG	20	20	55.0	50.0	58.0	57.9	11.8	1.5	0.166189	4223	4959	1581-1606	0.800000	74.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTCGGAGGCAGGTAAATTC	GGGGGTCTTGCTGATTCAAT	20	20	55.0	50.0	58.0	57.9	11.8	0.0	0.195046	4223	4959	1612-1615	0.600000	63.5
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ACGGAAAGAGAGGGATTCGA	GTAATCAAACCGGGAGAGACC	20	21	50.0	52.4	58.2	58.1	0.0	6.3	1.248826	8050	8887	1007-1101	1.000000	75.2
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	CGAATGACTCGTACAACGGA	GTAATCAAACCGGGAGAGACC	20	21	50.0	52.4	57.7	58.1	0.0	6.3	1.349347	8050	8887	977-1071	1.000000	74.5
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	TGGAAACGGAAAGAGAGGGA	GTAATCAAACCGGGAGAGACC	20	21	50.0	52.4	58.3	58.1	0.0	6.3	1.372933	8050	8887	1012-1106	1.000000	74.1
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ACGGAAAGAGAGGGATTCGA	TAATCAAACCGGGAGAGACC	20	20	50.0	50.0	58.2	56.6	0.0	6.3	1.516103	8050	8887	1006-1100	1.000000	73.0
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	CGAATGACTCGTACAACGGA	TAATCAAACCGGGAGAGACC	20	20	50.0	50.0	57.7	56.6	0.0	6.3	1.616624	8050	8887	976-1070	1.000000	72.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	ATCGGAATGAAAAGCGTCCA	TGGAACGCTTTCTACTTCAAGA	20	22	45.0	40.9	57.9	57.7	0.0	2.7	2.438894	9871	9960	201-208	1.000000	69.5
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TCGGAATGAAAAGCGTCCAT	TGGAACGCTTTCTACTTCAAGA	20	22	45.0	40.9	57.9	57.7	0.0	2.7	2.438894	9871	9960	200-207	1.000000	69.5
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	ATCGGAATGAAAAGCGTCCA	TGGAACGCTTTCTACTTCAAG	20	21	45.0	42.9	57.9	56.2	0.0	2.7	2.896478	9871	9960	201-208	1.000000	65.8
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TCGGAATGAAAAGCGTCCAT	TGGAACGCTTTCTACTTCAAG	20	21	45.0	42.9	57.9	56.2	0.0	2.7	2.896478	9871	9960	200-207	1.000000	65.8
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TCGGAATGAAAAGCGTCCAT	GGAACGCTTTCTACTTCAAGAAC	20	23	45.0	43.5	57.9	58.0	0.0	2.7	3.127857	9871	9960	199-206	1.000000	64.0
atpA-atpF_p1	atpA-atpF	1	GGGCGCAGGAGATTCAATTA	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.042826	11485	11547	976-978	1.000000	82.1
atpA-atpF_p2	atpA-atpF	2	GGGCGCAGGAGATTCAATTA	TCAAGTTTGGTTCGGGAAGG	20	20	50.0	50.0	58.0	58.0	10.8	0.0	0.057022	11485	11547	941-943	1.000000	82.0
atpA-atpF_p3	atpA-atpF	3	GGGCGCAGGAGATTCAATTA	TCAGAAGAACTTCGCGGAAG	20	20	50.0	50.0	58.0	57.9	10.8	6.1	0.098674	11485	11547	823-825	1.000000	81.8
atpA-atpF_p4	atpA-atpF	4	CACCGGCCATTACTTCATCA	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047103	11485	11547	727-727	0.800000	73.8
atpA-atpF_p5	atpA-atpF	5	CACCGGCCATTACTTCATCA	TCAAGTTTGGTTCGGGAAGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061298	11485	11547	692-692	0.800000	73.8
petN-psbM_p1	petN-psbM	1	ATTGTAAGTCTCGCTTGGGC	AAAACAGTCAGCCAAAATGA	20	20	50.0	35.0	58.3	53.9	0.0	0.0	4.379367	28238	28961	829-841	1.000000	49.3
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	AAAACAGTCAGCCAAAATGA	20	20	50.0	35.0	57.6	53.9	0.0	0.0	4.469296	28238	28961	767-779	1.000000	48.9
petN-psbM_p3	petN-psbM	3	ATTGTAAGTCTCGCTTGGGC	TGTAAAAACAGTCAGCCAAAATGA	20	24	50.0	33.3	58.3	57.8	0.0	0.0	4.475124	28238	28961	833-845	1.000000	48.5
petN-psbM_p4	petN-psbM	4	ATTGTAAGTCTCGCTTGGGC	AAAAACAGTCAGCCAAAATGA	20	21	50.0	33.3	58.3	54.8	0.0	0.0	4.505902	28238	28961	830-842	1.000000	48.3
petN-psbM_p5	petN-psbM	5	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTATTCATTCT	20	21	50.0	38.1	58.3	54.7	0.0	0.0	4.522800	28238	28961	884-896	1.000000	47.9
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.789957	30268	30638	434-457	1.000000	81.0
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.6	57.8	7.1	0.0	0.791509	30268	30638	433-456	1.000000	81.0
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.902511	30268	30638	455-478	1.000000	79.8
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.6	57.6	7.1	0.0	0.926188	30268	30638	454-477	1.000000	79.6
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GCGGTGCTCTAACCAATTGA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.6	58.4	7.1	0.0	0.963468	30268	30638	488-511	1.000000	78.8
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	AGTAGAATATTATTCTCGTCAGACT	20	25	50.0	32.0	58.1	54.9	0.0	19.7	8.147390	45065	45413	416-437	1.000000	44.9
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	AGGCTTTTGTTTACCGAGGG	AGAATATTATTCTCGTCAGACTT	20	23	50.0	30.4	58.1	52.8	0.0	12.3	8.279361	45065	45413	413-434	1.000000	44.9
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGCTTTTGTTTACCGAGGGT	AGTAGAATATTATTCTCGTCAGACT	20	25	50.0	32.0	58.4	54.9	0.0	19.7	8.440351	45065	45413	415-436	1.000000	44.9
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	AGGCTTTTGTTTACCGAGGG	AGTAGAATATTATTCTCGTCAGACTT	20	26	50.0	30.8	58.1	55.6	0.0	19.7	8.474897	45065	45413	416-437	1.000000	44.9
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	AGGCTTTTGTTTACCGAGGG	GTAGAATATTATTCTCGTCAGACT	20	24	50.0	33.3	58.1	53.6	0.0	19.7	8.511064	45065	45413	415-436	1.000000	44.9
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GGCGAGAGTCATTGGTTCAA	CACGAGTGGAAGCTAGCAAT	20	20	50.0	50.0	58.2	58.0	0.0	18.8	0.207325	52355	52655	352-368	1.000000	89.2
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	GGCGAGAGTCATTGGTTCAA	GACGAGCTAGAACACGAGTG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.306833	52355	52655	364-380	1.000000	88.2
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	ATTGCTTTCATACGGCGAGA	CACGAGTGGAAGCTAGCAAT	20	20	45.0	50.0	57.7	58.0	16.6	18.8	0.325264	52355	52655	365-381	1.000000	88.0
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	GGCGAGAGTCATTGGTTCAA	CAGACGAGCTAGAACACGAG	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.358330	52355	52655	366-382	1.000000	87.8
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	GGCGAGAGTCATTGGTTCAA	CTCAGACGAGCTAGAACACG	20	20	50.0	55.0	58.2	57.8	0.0	0.0	0.358330	52355	52655	368-384	1.000000	87.7
petA-psbJ_p1	petA-psbJ	1	CGAGAAGGTTCAACTGTCCG	CTGGATTAGGCTCATCACTGT	20	21	55.0	47.6	58.6	57.2	0.0	10.6	2.415115	62775	63745	1026-1036	1.000000	65.0
petA-psbJ_p2	petA-psbJ	2	GAGAAGGTTCAACTGTCCGA	CTGGATTAGGCTCATCACTGT	20	21	50.0	47.6	57.2	57.2	0.0	10.6	2.648769	62775	63745	1025-1035	1.000000	63.2
petA-psbJ_p3	petA-psbJ	3	TCGAGAAGGTTCAACTGTCC	CTGGATTAGGCTCATCACTGT	20	21	50.0	47.6	57.2	57.2	0.0	10.6	2.648769	62775	63745	1027-1037	1.000000	63.2
petA-psbJ_p4	petA-psbJ	4	CGAGAAGGTTCAACTGTCCG	TGGATTAGGCTCATCACTGT	20	20	55.0	45.0	58.6	55.9	0.0	10.6	2.657273	62775	63745	1025-1035	1.000000	63.1
petA-psbJ_p5	petA-psbJ	5	CGAGAAGGTTCAACTGTCCG	TCTGGATTAGGCTCATCACTG	20	21	55.0	47.6	58.6	56.9	0.0	10.6	2.692605	62775	63745	1027-1037	1.000000	62.8
rps3_p1	rps3	1	AGTCGTTATTGGTCCGAAGC	CGAGCTCGAGGACGTAGTTA	20	20	50.0	55.0	58.0	58.7	0.0	32.1	0.721314	82523	83182	1490-1576	1.000000	77.9
rps3_p2	rps3	2	GTCGTTATTGGTCCGAAGCT	CGAGCTCGAGGACGTAGTTA	20	20	50.0	55.0	58.0	58.7	0.0	32.1	0.721314	82523	83182	1489-1575	1.000000	77.9
rps3_p3	rps3	3	TGTGATGCGATGAGTTGGTT	CGAGCTCGAGGACGTAGTTA	20	20	45.0	55.0	57.8	58.7	0.0	32.1	0.898821	82523	83182	1180-1265	1.000000	76.7
rps3_p4	rps3	4	AGTCGTTATTGGTCCGAAGC	TCTCACTCCGGAACATTTCC	20	20	50.0	50.0	58.0	57.2	0.0	5.2	0.768506	82523	83182	1386-1387	0.600000	58.2
rps3_p5	rps3	5	GTCGTTATTGGTCCGAAGCT	TCTCACTCCGGAACATTTCC	20	20	50.0	50.0	58.0	57.2	0.0	5.2	0.768506	82523	83182	1385-1386	0.600000	58.2
rpl22-rps19_p1	rpl22-rps19	1	TCGTGCTTTGTCAACAGACA	AAGCTTAACACAAAGGCGGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.041478	83830	83894	469-473	1.000000	84.6
rpl22-rps19_p2	rpl22-rps19	2	TCGTGCTTTGTCAACAGACA	AGCTTAACACAAAGGCGGAA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.041478	83830	83894	468-472	1.000000	84.6
rpl22-rps19_p3	rpl22-rps19	3	CAAGTCGGAAACCAAGTGGA	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	3.8	29.9	0.041408	83830	83894	1318-1401	1.000000	83.8
rpl22-rps19_p4	rpl22-rps19	4	TCGTGCTTTGTCAACAGACA	TGGGTAGAGCCGGATCTAAG	20	20	45.0	55.0	58.0	58.0	0.0	29.9	0.020340	83830	83894	819-825	1.000000	83.4
rpl22-rps19_p5	rpl22-rps19	5	TCGTGCTTTGTCAACAGACA	CGTCCCGTAGTAAGAGGAGT	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.052668	83830	83894	784-790	1.000000	83.3
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CGGGAGAATCAATGACTGCA	TGGAGGAGGAGGTCGAATTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.075798	107079	108610	1964-2317	1.000000	90.6
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGCAGAATAGCAGAGCAAGT	TGGAGGAGGAGGTCGAATTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082909	107079	108610	2060-2413	1.000000	90.4
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CGGGAGAATCAATGACTGCA	GTATGGAGGAGGAGGTCGAA	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.097254	107079	108610	1967-2320	1.000000	90.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CGGGAGAATCAATGACTGCA	AAGTATGGAGGAGGAGGTCG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.103148	107079	108610	1969-2322	1.000000	90.3
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	TGGGTCTTTTGTCGAAGTGG	TGGAGGAGGAGGTCGAATTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.085585	107079	108610	2207-2560	1.000000	90.2
ndhF-rpl32_p1	ndhF-rpl32	1	AGGGTATGATCCATGCATATTGA	ACGCTTTTTGTAAATTGAAGTACG	23	24	39.1	33.3	57.2	57.0	0.0	0.0	8.747415	110837	111882	1125-1136	1.000000	45.0
ndhF-rpl32_p2	ndhF-rpl32	2	AGGGTATGATCCATGCATATTGA	CGCTTTTTGTAAATTGAAGTACGT	23	24	39.1	33.3	57.2	57.0	0.0	0.0	8.747415	110837	111882	1124-1135	1.000000	45.0
ndhF-rpl32_p3	ndhF-rpl32	3	AAGGGTATGATCCATGCATATTGA	ACGCTTTTTGTAAATTGAAGTACG	24	24	37.5	33.3	57.9	57.0	0.0	0.0	9.106568	110837	111882	1126-1137	1.000000	45.0
ndhF-rpl32_p4	ndhF-rpl32	4	AAGGGTATGATCCATGCATATTGA	CGCTTTTTGTAAATTGAAGTACGT	24	24	37.5	33.3	57.9	57.0	0.0	0.0	9.106568	110837	111882	1125-1136	1.000000	45.0
ndhF-rpl32_p5	ndhF-rpl32	5	AGGGTATGATCCATGCATATTGA	CGCTTTTTGTAAATTGAAGTACGTT	23	25	39.1	32.0	57.2	57.6	0.0	0.0	9.155737	110837	111882	1124-1135	1.000000	45.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCCTTTT	CTCGCACTGCTTCCTAAGAG	20	20	45.0	55.0	57.9	58.1	7.7	0.0	0.228997	112033	112807	898-918	1.000000	83.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCCTTTT	ATCCGCATAAAAGAGCTGCA	20	20	45.0	45.0	57.9	58.0	7.7	1.0	0.191602	112033	112807	1531-1556	1.000000	82.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTAAAAGCCTTTT	TCCGCATAAAAGAGCTGCAT	20	20	45.0	45.0	57.9	58.0	7.7	1.0	0.191602	112033	112807	1530-1555	1.000000	82.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCGTTAAAAGCCTTTT	TCCGACGTAGCAAATCCTTG	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.153498	112033	112807	1418-1426	0.800000	74.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTAAAAGCCTTTT	GTCCGACGTAGCAAATCCTT	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.154886	112033	112807	1419-1427	0.800000	74.4
ycf1_p1	ycf1	1	GCTCTACCCACATGTCGTTT	CAGCAAGAAGAGGGATGCAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.148994	126528	126647	1171-1183	0.600000	63.4
ycf1_p2	ycf1	2	GATGCTCTACCCACATGTCG	CAGCAAGAAGAGGGATGCAT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.196141	126528	126647	1174-1186	0.600000	63.0
ycf1_p3	ycf1	3	GCTCTACCCACATGTCGTTT	AACAGCAAGAAGAGGGATGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.263182	126528	126647	1173-1185	0.600000	62.4
ycf1_p4	ycf1	4	TCAAAACGATATCGCCACGT	CAGCAAGAAGAGGGATGCAT	20	20	45.0	50.0	58.0	57.9	24.6	0.0	0.055184	126528	126647	1288-1288	0.200000	47.6
ycf1_p5	ycf1	5	TCAAAACGATATCGCCACGT	AACAGCAAGAAGAGGGATGC	20	20	45.0	50.0	58.0	58.2	24.6	0.0	0.169371	126528	126647	1290-1290	0.200000	46.6