primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CGTGCTAACCTTGGTATGGA	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.812921	79	521	557-644	1.000000	81.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TCGTGCTAACCTTGGTATGG	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.812921	79	521	558-645	1.000000	81.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CGTGCTAACCTTGGTATGGA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.817283	79	521	571-658	1.000000	81.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	TCGTGCTAACCTTGGTATGG	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.817283	79	521	572-659	1.000000	81.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTGATCCAC	CGTGCTAACCTTGGTATGGA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.818790	79	521	570-657	1.000000	81.1
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	13080	14090	1090-1156	1.000000	81.0
atpH-atpI_p2	atpH-atpI	2	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.347127	13080	14090	1107-1173	1.000000	80.1
atpH-atpI_p3	atpH-atpI	3	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.394902	13080	14090	1102-1168	1.000000	79.7
atpH-atpI_p4	atpH-atpI	4	AATCCAGCAGCAATAACGGA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.6	57.9	0.0	0.0	0.551108	13080	14090	1104-1170	1.000000	78.5
atpH-atpI_p5	atpH-atpI	5	ATCCAGCAGCAATAACGGAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.6	57.9	0.0	0.0	0.551108	13080	14090	1103-1169	1.000000	78.5
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28515	29503	1064-1081	1.000000	76.9
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28515	29503	1062-1079	1.000000	76.9
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TCTAGTTCCTACCGCCTTTCT	20	21	55.0	47.6	57.9	57.9	0.0	0.0	1.177628	28515	29503	1061-1078	1.000000	76.3
petN-psbM_p4	petN-psbM	4	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCCTTTCTACT	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.187705	28515	29503	1061-1078	1.000000	76.2
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCCTTTCTACT	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.187705	28515	29503	1059-1076	1.000000	76.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	31150	32496	1483-1542	1.000000	83.7
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	31150	32496	1486-1545	1.000000	83.5
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	31150	32496	1473-1532	1.000000	82.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	31150	32496	1476-1535	1.000000	82.5
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	31150	32496	1460-1519	1.000000	82.1
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CATTTGCTTCTCCTGGTGGT	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.143534	35767	35981	382-387	1.000000	84.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CATTTGCTTCTCCTGGTGGT	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.143534	35767	35981	383-388	1.000000	84.9
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GTTGTATTTTCCGGCGCATC	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.2	58.1	10.8	0.0	0.236094	35767	35981	344-349	1.000000	84.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GTTGTATTTTCCGGCGCATC	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.2	58.1	10.8	0.0	0.236094	35767	35981	345-350	1.000000	84.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCCTGGTGGTTGGTCAAGTA	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.249542	35767	35981	372-377	1.000000	84.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	TCGAAGATCCGTTTCGTGAC	GCTTCCATTGAGTCTCTGCA	20	20	50.0	50.0	58.0	57.9	13.3	0.6	0.131868	45937	46596	1094-1111	1.000000	82.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TCGAAGATCCGTTTCGTGAC	TGCCCAAATACGTGTGTCAA	20	20	50.0	45.0	58.0	58.0	13.3	0.0	0.058157	45937	46596	1569-1585	1.000000	82.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TCGAAGATCCGTTTCGTGAC	AGGAATTTGGACCCGCTAAC	20	20	50.0	50.0	58.0	57.9	13.3	0.0	0.151139	45937	46596	1533-1549	1.000000	81.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TGCCCAAATACGTGTGTCAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.164036	45937	46596	1366-1382	1.000000	81.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TCGAAGATCCGTTTCGTGAC	GAGTCTCTGCACCTATCCCT	20	20	50.0	55.0	58.0	57.9	13.3	0.0	0.107516	45937	46596	1085-1095	0.600000	65.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.7	0.0	0.279423	49901	50990	1159-1242	1.000000	84.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.7	0.0	0.623195	49901	50990	1158-1241	1.000000	81.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	49901	50990	1147-1230	1.000000	80.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAATCCCGGAAAT	AAGGTCTACGGTTCGAGTCC	20	20	50.0	55.0	57.9	58.8	0.7	0.0	0.909965	49901	50990	1157-1240	1.000000	79.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAATCCCGGAAAT	GAGAAGGTCTACGGTTCGAG	20	20	50.0	55.0	57.9	57.2	0.7	0.0	0.911014	49901	50990	1160-1243	1.000000	79.0
rps19-rpl2_p1	rps19-rpl2	1	AGATGAAGGGCTACAAAAGGA	GATTGTTCGTCGCCGTAGTA	21	20	42.9	50.0	56.6	57.8	0.0	0.0	2.559668	83632	83691	121-132	1.000000	66.7
rps19-rpl2_p2	rps19-rpl2	2	AGATGAAGGGCTACAAAAGGA	TTTGATTGTTCGTCGCCGTA	21	20	42.9	45.0	56.6	58.2	0.0	0.0	2.600388	83632	83691	124-135	1.000000	66.3
rps19-rpl2_p3	rps19-rpl2	3	AGATGAAGGGCTACAAAAGGA	AATTTGATTGTTCGTCGCCG	21	20	42.9	45.0	56.6	57.8	0.0	0.0	2.622453	83632	83691	126-137	1.000000	66.1
rps19-rpl2_p4	rps19-rpl2	4	AGATGAAGGGCTACAAAAGGA	TTGTTCGTCGCCGTAGTAAA	21	20	42.9	45.0	56.6	57.6	0.0	0.0	2.795252	83632	83691	119-130	1.000000	64.9
rps19-rpl2_p5	rps19-rpl2	5	AGATGAAGGGCTACAAAAGGA	TGTTCGTCGCCGTAGTAAAT	21	20	42.9	45.0	56.6	57.4	0.0	0.0	3.016176	83632	83691	118-129	1.000000	63.1
ndhF-rpl32_p1	ndhF-rpl32	1	CAAGAGGTCGTGTGAACCAA	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.076236	111165	111579	1014-1089	1.000000	83.2
ndhF-rpl32_p2	ndhF-rpl32	2	AAACCAAAGTCCCCTACACG	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.289929	111165	111579	1069-1144	1.000000	81.3
ndhF-rpl32_p3	ndhF-rpl32	3	ACCAAAGTCCCCTACACGAT	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.371393	111165	111579	1067-1142	1.000000	80.7
ndhF-rpl32_p4	ndhF-rpl32	4	ACATGAAGCGGAAATTGTGC	TTAACGCTGCCCAATATCCC	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.376843	111165	111579	1246-1321	1.000000	80.4
ndhF-rpl32_p5	ndhF-rpl32	5	CAAGAGGTCGTGTGAACCAA	GCTGCCCAATATCCCTTTCT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.423554	111165	111579	1009-1084	1.000000	80.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGGCAGCGTTAA	TTTAGGAGATGACATGCCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.070781	111736	112506	928-953	1.000000	87.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGATATTGGGCAGCGTTAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148618	111736	112506	873-898	1.000000	87.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGATATTGGGCAGCGTTAA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.209344	111736	112506	866-891	1.000000	87.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGATATTGGGCAGCGTTAA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.220635	111736	112506	871-896	1.000000	86.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGATATTGGGCAGCGTTAA	TGATCCACATAAAAGGGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075675	111736	112506	1463-1484	0.600000	66.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGGGTTAATATATGCTCTAAGGTTGA	20	26	50.0	34.6	57.9	57.7	0.0	0.0	6.394414	112587	112669	178-179	1.000000	43.1
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GGTAGACACGCTGCTCTTAG	TGGGTTAATATATGCTCTAAGGTTGA	20	26	55.0	34.6	57.8	57.7	0.0	0.0	6.466432	112587	112669	180-181	1.000000	43.1
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTAGACACGCTGCTCTTAGG	TGGGTTAATATATGCTCTAAGGTTGA	20	26	55.0	34.6	57.8	57.7	0.0	0.0	6.466432	112587	112669	179-180	1.000000	43.1
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	TGGGTTAATATATGCTCTAAGGTTGA	20	26	45.0	34.6	57.8	57.7	0.0	0.0	6.455141	112587	112669	185-186	1.000000	43.0
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AATTGGTAGACACGCTGCTC	TGGGTTAATATATGCTCTAAGGTTGA	20	26	50.0	34.6	58.3	57.7	0.0	0.0	6.543284	112587	112669	184-185	1.000000	43.0
ndhD_p1	ndhD	1	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	57.9	58.0	27.9	22.2	0.086024	113894	115396	2258-2261	1.000000	82.9
ndhD_p2	ndhD	2	TTGGAGTTGGGACCCAAAAG	AAGATGTGAATCCGCCTGTC	20	20	50.0	50.0	57.9	58.0	27.9	0.0	0.110492	113894	115396	2186-2189	1.000000	82.8
ndhD_p3	ndhD	3	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	20	20	55.0	55.0	58.1	58.0	1.9	22.2	0.121135	113894	115396	2280-2283	1.000000	82.6
ndhD_p4	ndhD	4	CGCCAGGTCAAATTGCATTT	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	57.9	58.0	0.0	22.2	0.088537	113894	115396	1931-1933	1.000000	82.5
ndhD_p5	ndhD	5	CGCCAGGTCAAATTGCATTT	AAGATGTGAATCCGCCTGTC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.113005	113894	115396	1859-1861	1.000000	82.4
ndhD-psaC_p1	ndhD-psaC	1	CCCGTCAGTAAAATGGGTCC	GATACCCTGGGACGGATGTA	20	20	55.0	55.0	58.3	58.0	0.0	22.2	0.258900	115397	115525	572-573	1.000000	82.3
ndhD-psaC_p2	ndhD-psaC	2	CGAATCTCGAGTAACTGGCC	AAGATGTGAATCCGCCTGTC	20	20	55.0	50.0	58.2	58.0	13.1	0.0	0.188389	115397	115525	552-553	0.600000	65.1
ndhD-psaC_p3	ndhD-psaC	3	CGAATCTCGAGTAACTGGCC	GATACCCTGGGACGGATGTA	20	20	55.0	55.0	58.2	58.0	13.1	22.2	0.163921	115397	115525	624-625	0.600000	65.0
ndhD-psaC_p4	ndhD-psaC	4	CGAATCTCGAGTAACTGGCC	AACAAATCGCTTCTGCTCCA	20	20	55.0	45.0	58.2	58.1	13.1	0.0	0.262331	115397	115525	598-599	0.600000	64.3
ndhD-psaC_p5	ndhD-psaC	5	CGAATCTCGAGTAACTGGCC	ACAAATCGCTTCTGCTCCAA	20	20	55.0	45.0	58.2	58.1	13.1	0.0	0.262331	115397	115525	597-598	0.600000	64.3
ndhA_p1	ndhA	1	CGTGGGACAGGGTAATCATG	GCCGGGTTTTATCAACTTGC	20	20	55.0	50.0	58.0	58.0	0.0	2.2	0.055642	117951	120116	2452-2465	1.000000	82.8
ndhA_p2	ndhA	2	CGTGGGACAGGGTAATCATG	CAATGTTACGAGCTTCCGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.051024	117951	120116	2855-2868	1.000000	82.4
ndhA_p3	ndhA	3	ATTCTCGCCAACCAACCATT	GCCGGGTTTTATCAACTTGC	20	20	45.0	50.0	58.1	58.0	0.0	2.2	0.085845	117951	120116	2965-2978	1.000000	82.3
ndhA_p4	ndhA	4	CGTGGGACAGGGTAATCATG	CGAGTTGAAGGCATAGGCAT	20	20	55.0	50.0	58.0	58.0	0.0	0.1	0.087399	117951	120116	2916-2929	1.000000	82.1
ndhA_p5	ndhA	5	CGTGGGACAGGGTAATCATG	CCGGGGTTGCTGAATATCAA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.097775	117951	120116	2969-2982	1.000000	82.0
ycf1_p1	ycf1	1	TCGTTTCCATCACTTTGGCA	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.064122	126555	126674	370-376	0.800000	79.3
ycf1_p2	ycf1	2	TCGTTTCCATCACTTTGGCA	ATTATGGACCCGCTACCAGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.122845	126555	126674	739-745	0.800000	75.4
ycf1_p3	ycf1	3	TCGTTTCCATCACTTTGGCA	GGCCCTCCTATCTCTTCCTT	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.136488	126555	126674	961-967	0.800000	74.3
ycf1_p4	ycf1	4	TTCATGGGTCCTGGTTCTCT	GCAGAATACCGTCACCCATT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.083179	126555	126674	883-898	0.600000	67.2
ycf1_p5	ycf1	5	TTCATGGGTCCTGGTTCTCT	ATTATGGACCCGCTACCAGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.141903	126555	126674	1252-1267	0.600000	65.7