primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gln-tRNA-Ser_p1	tRNA-Gln-tRNA-Ser	1	GATCAAAACCCGCTGCCTTA	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.5	58.2	0.0	17.2	0.757478	7825	9017	1251-1272	1.000000	78.9
tRNA-Gln-tRNA-Ser_p2	tRNA-Gln-tRNA-Ser	2	GATCAAAACCCGCTGCCTTA	TAACGCGTCGGATTGCTAAT	20	20	50.0	45.0	58.5	57.8	0.0	14.4	0.753419	7825	9017	1296-1317	1.000000	78.8
tRNA-Gln-tRNA-Ser_p3	tRNA-Gln-tRNA-Ser	3	GATCAAAACCCGCTGCCTTA	CTAACGCGTCGGATTGCTAA	20	20	50.0	50.0	58.5	58.4	0.0	14.4	0.988324	7825	9017	1297-1318	1.000000	76.9
tRNA-Gln-tRNA-Ser_p4	tRNA-Gln-tRNA-Ser	4	GATCAAAACCCGCTGCCTTA	TTTTGCTTGGAGAGATGGCC	20	20	50.0	50.0	58.5	58.5	0.0	0.0	0.991346	7825	9017	1324-1345	1.000000	76.7
tRNA-Gln-tRNA-Ser_p5	tRNA-Gln-tRNA-Ser	5	CGAATGACGGGATCAAAACC	TAACGCGTCGGATTGCTAAT	20	20	50.0	45.0	57.2	57.8	0.0	14.4	1.037955	7825	9017	1306-1327	1.000000	76.4
tRNA-Gly-tRNA-Gly_p1	tRNA-Gly-tRNA-Gly	1	TTGTGGCGGGTATGGTTTAG	CCCGCATCATTAGCTTGGAA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.437688	9930	10784	910-932	1.000000	81.0
tRNA-Gly-tRNA-Gly_p2	tRNA-Gly-tRNA-Gly	2	TTAGACAGAGGACCCGTCTG	CCCGCATCATTAGCTTGGAA	20	20	55.0	50.0	58.2	58.3	15.7	0.0	0.485367	9930	10784	945-967	1.000000	80.5
tRNA-Gly-tRNA-Gly_p3	tRNA-Gly-tRNA-Gly	3	AGAGGACCCGTCTGTGTAAT	CCCGCATCATTAGCTTGGAA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.554166	9930	10784	939-961	1.000000	80.0
tRNA-Gly-tRNA-Gly_p4	tRNA-Gly-tRNA-Gly	4	TTGTGGCGGGTATGGTTTAG	ATCGAACCCGCATCATTAGC	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.603901	9930	10784	916-938	1.000000	79.7
tRNA-Gly-tRNA-Gly_p5	tRNA-Gly-tRNA-Gly	5	TTAGACAGAGGACCCGTCTG	ATCGAACCCGCATCATTAGC	20	20	55.0	50.0	58.2	58.5	15.7	0.0	0.651580	9930	10784	951-973	1.000000	79.1
tRNA-Arg-tRNA-Asp_p1	tRNA-Arg-tRNA-Asp	1	AAACAAAATCCACCGACGGA	AATCCAGAAACTCGACGAGC	20	20	45.0	50.0	58.0	58.0	23.4	24.4	0.032239	27991	28140	435-447	0.600000	70.7
tRNA-Arg-tRNA-Asp_p2	tRNA-Arg-tRNA-Asp	2	AAACAAAATCCACCGACGGA	CAGAAACTCGACGAGCAGAA	20	20	45.0	50.0	58.0	57.9	23.4	24.4	0.081787	27991	28140	431-443	0.600000	70.3
tRNA-Arg-tRNA-Asp_p3	tRNA-Arg-tRNA-Asp	3	AGTTCTGGTAGTACAGGCGT	AATCCAGAAACTCGACGAGC	20	20	50.0	50.0	58.1	58.0	2.1	24.4	0.081721	27991	28140	678-692	0.600000	68.7
tRNA-Arg-tRNA-Asp_p4	tRNA-Arg-tRNA-Asp	4	CCTTGTGAATCCATCCAGGG	AATCCAGAAACTCGACGAGC	20	20	55.0	50.0	57.9	58.0	0.0	24.4	0.065301	27991	28140	966-980	0.600000	67.8
tRNA-Arg-tRNA-Asp_p5	tRNA-Arg-tRNA-Asp	5	CTTGTGAATCCATCCAGGGG	AATCCAGAAACTCGACGAGC	20	20	55.0	50.0	57.9	58.0	0.0	24.4	0.065301	27991	28140	965-979	0.600000	67.8
tRNA-Tyr-tRNA-Glu_p1	tRNA-Tyr-tRNA-Glu	1	GTCCCCATTAACCACTCGAG	CGTCTAGAGGCCTAGGACAA	20	20	55.0	55.0	57.7	57.9	11.8	19.5	0.365356	28877	29055	269-273	1.000000	84.5
tRNA-Tyr-tRNA-Glu_p2	tRNA-Tyr-tRNA-Glu	2	CCCATTAACCACTCGAGCAT	CGTCTAGAGGCCTAGGACAA	20	20	50.0	55.0	57.7	57.9	13.6	19.5	0.386295	28877	29055	266-270	1.000000	84.4
tRNA-Tyr-tRNA-Glu_p3	tRNA-Tyr-tRNA-Glu	3	GTCCCCATTAACCACTCGAG	GAGGCCTAGGACAACTCTCT	20	20	55.0	55.0	57.7	57.8	11.8	19.5	0.464158	28877	29055	263-267	1.000000	83.9
tRNA-Tyr-tRNA-Glu_p4	tRNA-Tyr-tRNA-Glu	4	GTCCCCATTAACCACTCGAG	AGAGGCCTAGGACAACTCTC	20	20	55.0	55.0	57.7	57.8	11.8	19.5	0.464158	28877	29055	264-268	1.000000	83.8
tRNA-Tyr-tRNA-Glu_p5	tRNA-Tyr-tRNA-Glu	5	CCCATTAACCACTCGAGCAT	GAGGCCTAGGACAACTCTCT	20	20	50.0	55.0	57.7	57.8	13.6	19.5	0.485097	28877	29055	260-264	1.000000	83.8
tRNA-Glu-tRNA-Cys_p1	tRNA-Glu-tRNA-Cys	1	TTGTCCTAGGCCTCTAGACG	CCTTGATTCCCCAGTTCGAG	20	20	55.0	55.0	57.9	58.0	9.7	0.0	0.084070	29129	30535	1462-1474	1.000000	84.5
tRNA-Glu-tRNA-Cys_p2	tRNA-Glu-tRNA-Cys	2	TTGTCCTAGGCCTCTAGACG	TGATTCCCCAGTTCGAGTCT	20	20	55.0	50.0	57.9	58.1	9.7	0.0	0.115647	29129	30535	1459-1471	1.000000	84.3
tRNA-Glu-tRNA-Cys_p3	tRNA-Glu-tRNA-Cys	3	AGAGAGTTGTCCTAGGCCTC	CCTTGATTCCCCAGTTCGAG	20	20	55.0	55.0	57.8	58.0	9.7	0.0	0.182872	29129	30535	1468-1480	1.000000	83.7
tRNA-Glu-tRNA-Cys_p4	tRNA-Glu-tRNA-Cys	4	GAGAGTTGTCCTAGGCCTCT	CCTTGATTCCCCAGTTCGAG	20	20	55.0	55.0	57.8	58.0	9.7	0.0	0.182872	29129	30535	1467-1479	1.000000	83.7
tRNA-Glu-tRNA-Cys_p5	tRNA-Glu-tRNA-Cys	5	GAGAGTTGTCCTAGGCCTCT	TGATTCCCCAGTTCGAGTCT	20	20	55.0	50.0	57.8	58.1	9.7	0.0	0.214449	29129	30535	1464-1476	1.000000	83.4
tRNA-Cys-tRNA-Gly_p1	tRNA-Cys-tRNA-Gly	1	CTCGAACTGGGGAATCAAGG	GTTCGATTCCCGCTATCCG	20	19	55.0	57.9	58.0	58.2	0.0	0.0	1.219352	30608	30896	360-368	1.000000	80.7
tRNA-Cys-tRNA-Gly_p2	tRNA-Cys-tRNA-Gly	2	CTCGAACTGGGGAATCAAGG	GGGTTCGATTCCCGCTATC	20	19	55.0	57.9	58.0	57.8	0.0	9.9	1.242288	30608	30896	362-370	1.000000	80.5
tRNA-Cys-tRNA-Gly_p3	tRNA-Cys-tRNA-Gly	3	CTCGAACTGGGGAATCAAGG	GGTTCGATTCCCGCTATCC	20	19	55.0	57.9	58.0	57.8	0.0	0.0	1.242288	30608	30896	361-369	1.000000	80.5
tRNA-Cys-tRNA-Gly_p4	tRNA-Cys-tRNA-Gly	4	AGACTCGAACTGGGGAATCA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.1	58.2	0.0	0.0	1.250930	30608	30896	363-371	1.000000	80.4
tRNA-Cys-tRNA-Gly_p5	tRNA-Cys-tRNA-Gly	5	TTTAGTAGGCGACACCCAGA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.1	58.2	0.0	0.0	1.262027	30608	30896	380-388	1.000000	79.9
tRNA-Thr-tRNA-Met_p1	tRNA-Thr-tRNA-Met	1	GAACCGATGACTTACGCCTT	GTTCAAATCCTGTCTCCGCA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.197560	36565	36723	235-367	1.000000	84.1
tRNA-Thr-tRNA-Met_p2	tRNA-Thr-tRNA-Met	2	GAACCGATGACTTACGCCTT	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.231039	36565	36723	268-400	1.000000	83.3
tRNA-Thr-tRNA-Met_p3	tRNA-Thr-tRNA-Met	3	GAACCGATGACTTACGCCTT	TATCGATTACGCGGGGTAGA	20	20	50.0	50.0	58.0	57.8	0.0	12.5	0.189449	36565	36723	297-429	1.000000	83.2
tRNA-Thr-tRNA-Met_p4	tRNA-Thr-tRNA-Met	4	GAACCGATGACTTACGCCTT	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.231039	36565	36723	269-401	1.000000	83.2
tRNA-Thr-tRNA-Met_p5	tRNA-Thr-tRNA-Met	5	GAACCGATGACTTACGCCTT	GTATCGATTACGCGGGGTAG	20	20	50.0	55.0	58.0	57.7	0.0	12.5	0.259363	36565	36723	298-430	1.000000	82.6
tRNA-Ser-tRNA-Leu_p1	tRNA-Ser-tRNA-Leu	1	CGAGGGGTTTAAGGTGTAGC	CTGAATCTGGGAGCTGTCAC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.041303	44853	46444	1751-1764	1.000000	85.3
tRNA-Ser-tRNA-Leu_p2	tRNA-Ser-tRNA-Leu	2	CGAGGGGTTTAAGGTGTAGC	TCGATCCGATTGAGTCCGTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.062279	44853	46444	1708-1721	1.000000	85.3
tRNA-Ser-tRNA-Leu_p3	tRNA-Ser-tRNA-Leu	3	CGAGGGGTTTAAGGTGTAGC	GATCCGATTGAGTCCGTAGC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.073230	44853	46444	1706-1719	1.000000	85.2
tRNA-Ser-tRNA-Leu_p4	tRNA-Ser-tRNA-Leu	4	GCTGTTTACCGAGGGTTCAA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.134469	44853	46444	1710-1723	1.000000	84.7
tRNA-Ser-tRNA-Leu_p5	tRNA-Ser-tRNA-Leu	5	CGAGGGGTTTAAGGTGTAGC	GTCCGTAGCGTCTACCATTC	20	20	55.0	55.0	58.0	57.9	0.0	10.2	0.136735	44853	46444	1695-1708	1.000000	84.7
tRNA-Leu-tRNA-Phe_p1	tRNA-Leu-tRNA-Phe	1	GCGAGCGTAACAATGCAAAT	GACTTGAACTGGTGACACGA	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.323902	47033	47399	507-515	1.000000	83.2
tRNA-Leu-tRNA-Phe_p2	tRNA-Leu-tRNA-Phe	2	GCGAGCGTAACAATGCAAAT	ACTTGAACTGGTGACACGAG	20	20	45.0	50.0	58.1	57.8	0.0	15.3	0.330017	47033	47399	506-514	1.000000	83.2
tRNA-Leu-tRNA-Phe_p3	tRNA-Leu-tRNA-Phe	3	GCGAGCGTAACAATGCAAAT	AGACTTGAACTGGTGACACG	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.330017	47033	47399	508-516	1.000000	83.1
tRNA-Leu-tRNA-Phe_p4	tRNA-Leu-tRNA-Phe	4	GCGAGCGTAACAATGCAAAT	GAACTGGTGACACGAGGATT	20	20	45.0	50.0	58.1	57.5	0.0	20.1	0.562315	47033	47399	502-510	1.000000	81.4
tRNA-Leu-tRNA-Phe_p5	tRNA-Leu-tRNA-Phe	5	GCGAGCGTAACAATGCAAAT	CTTGAACTGGTGACACGAGG	20	20	45.0	55.0	58.1	58.5	0.0	20.1	0.602718	47033	47399	505-513	1.000000	81.0
tRNA-Val-tRNA-Met_p1	tRNA-Val-tRNA-Met	1	CGTGTAAACGAGGTGCTCTA	CGAAACTCTAACCGCTGAGT	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.973613	50528	50737	263-277	1.000000	82.7
tRNA-Val-tRNA-Met_p2	tRNA-Val-tRNA-Met	2	CGTGTAAACGAGGTGCTCTA	TTCGAACCAATGACTCTCGC	20	20	50.0	50.0	57.4	58.3	0.0	0.0	0.914303	50528	50737	293-307	1.000000	82.5
tRNA-Val-tRNA-Met_p3	tRNA-Val-tRNA-Met	3	CGTGTAAACGAGGTGCTCTA	GCCGTATGAAAGCGAAACTC	20	20	50.0	50.0	57.4	57.6	0.0	0.0	1.061624	50528	50737	275-289	1.000000	81.7
tRNA-Val-tRNA-Met_p4	tRNA-Val-tRNA-Met	4	CGTGTAAACGAGGTGCTCTA	GAAAGCGAAACTCTAACCGC	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.129924	50528	50737	268-282	1.000000	81.3
tRNA-Val-tRNA-Met_p5	tRNA-Val-tRNA-Met	5	CGTGTAAACGAGGTGCTCTA	CAATGACTCTCGCCGTATGA	20	20	50.0	50.0	57.4	57.5	0.0	0.0	1.116335	50528	50737	286-300	1.000000	81.0
tRNA-Trp-tRNA-Pro_p1	tRNA-Trp-tRNA-Pro	1	ACCTACGACATCGGGTTTTG	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	6.2	0.0	0.279274	63306	63471	268-278	1.000000	85.1
tRNA-Trp-tRNA-Pro_p2	tRNA-Trp-tRNA-Pro	2	AAACACGCCCTGTAGGATTC	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.299750	63306	63471	290-300	1.000000	84.4
tRNA-Trp-tRNA-Pro_p3	tRNA-Trp-tRNA-Pro	3	ACCTACGACATCGGGTTTTG	AGGTTCAAATCCCGTCATCC	20	20	50.0	50.0	57.9	57.6	6.2	0.0	0.509008	63306	63471	240-250	1.000000	84.1
tRNA-Trp-tRNA-Pro_p4	tRNA-Trp-tRNA-Pro	4	ACCTACGACATCGGGTTTTG	GTTCAAATCCCGTCATCCCT	20	20	50.0	50.0	57.9	57.6	6.2	0.0	0.509008	63306	63471	238-248	1.000000	84.1
tRNA-Trp-tRNA-Pro_p5	tRNA-Trp-tRNA-Pro	5	ACCTACGACATCGGGTTTTG	TAGCGCGTTTGTTTTGGGTA	20	20	50.0	45.0	57.9	58.4	6.2	8.4	0.498017	63306	63471	271-281	1.000000	83.2
tRNA-Pro-tRNA-Ile_p1	tRNA-Pro-tRNA-Ile	1	GCTAATCTCCCTTCCTTGGC	CATCACCATCAGACACAGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.059839	82118	82237	1054-1082	1.000000	86.9
tRNA-Pro-tRNA-Ile_p2	tRNA-Pro-tRNA-Ile	2	GATCCAGCCTTTCCTAAGCC	CATCACCATCAGACACAGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.059839	82118	82237	1168-1196	1.000000	86.6
tRNA-Pro-tRNA-Ile_p3	tRNA-Pro-tRNA-Ile	3	GATCCAGCCTTTCCTAAGCC	AAAAGATGTTGACCTGCCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.044479	82118	82237	1243-1243	0.200000	47.6
tRNA-Pro-tRNA-Ile_p4	tRNA-Pro-tRNA-Ile	4	GCTAATCTCCCTTCCTTGGC	AAAAGATGTTGACCTGCCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.044479	82118	82237	1129-1129	0.200000	47.6
tRNA-Pro-tRNA-Ile_p5	tRNA-Pro-tRNA-Ile	5	CGTGTAGATCCAGCCTTTCC	AAAAGATGTTGACCTGCCGT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.077419	82118	82237	1249-1249	0.200000	47.4
tRNA-Asn-tRNA-Pro_p1	tRNA-Asn-tRNA-Pro	1	CAAGAGCTCTTTCCTCAGCC	AACTCTGGCTATGTCCAAGC	20	20	55.0	50.0	58.3	57.6	26.4	0.0	0.673337	108201	108320	819-848	0.600000	69.4
tRNA-Asn-tRNA-Pro_p2	tRNA-Asn-tRNA-Pro	2	CAAGAGCTCTTTCCTCAGCC	ACTCTGGCTATGTCCAAGCT	20	20	55.0	50.0	58.3	58.4	26.4	0.0	0.685652	108201	108320	818-847	0.600000	69.3
tRNA-Asn-tRNA-Pro_p3	tRNA-Asn-tRNA-Pro	3	AACGAGAAAGCTTCTGGACC	AACTCTGGCTATGTCCAAGC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.581289	108201	108320	783-783	0.200000	43.3
tRNA-Asn-tRNA-Pro_p4	tRNA-Asn-tRNA-Pro	4	AACGAGAAAGCTTCTGGACC	ACTCTGGCTATGTCCAAGCT	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.593605	108201	108320	782-782	0.200000	43.3
tRNA-Asn-tRNA-Pro_p5	tRNA-Asn-tRNA-Pro	5	GAGAAAGCTTCTGGACCGAA	AACTCTGGCTATGTCCAAGC	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.867314	108201	108320	780-780	0.200000	41.1
tRNA-Pro-tRNA-Leu_p1	tRNA-Pro-tRNA-Leu	1	GATTCGAACCTGTGACCTCC	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135817	108527	108695	270-313	1.000000	84.2
tRNA-Pro-tRNA-Leu_p2	tRNA-Pro-tRNA-Leu	2	GGATTCGAACCTGTGACCTC	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.135817	108527	108695	271-314	1.000000	84.1
tRNA-Pro-tRNA-Leu_p3	tRNA-Pro-tRNA-Leu	3	GATTCGAACCTGTGACCTCC	CAGCGTGTCTACCAGTTTCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.160456	108527	108695	261-304	1.000000	84.1
tRNA-Pro-tRNA-Leu_p4	tRNA-Pro-tRNA-Leu	4	GGATTCGAACCTGTGACCTC	CAGCGTGTCTACCAGTTTCA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.160456	108527	108695	262-305	1.000000	84.1
tRNA-Pro-tRNA-Leu_p5	tRNA-Pro-tRNA-Leu	5	GATTCGAACCTGTGACCTCC	AGCGTGTCTACCAGTTTCAC	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.161937	108527	108695	260-303	1.000000	84.1
tRNA-Leu-tRNA-Asn_p1	tRNA-Leu-tRNA-Asn	1	GGATTTCCACTTCCACCGAG	TTTCTGCGAGATGGGCATAC	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.307374	119534	119653	825-975	1.000000	89.2
tRNA-Leu-tRNA-Asn_p2	tRNA-Leu-tRNA-Asn	2	GGATTTCCACTTCCACCGAG	ATTTTCTGCGAGATGGGCAT	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.323904	119534	119653	827-977	1.000000	89.0
tRNA-Leu-tRNA-Asn_p3	tRNA-Leu-tRNA-Asn	3	GGATTTCCACTTCCACCGAG	CTGCCTTGGATGCCCATAAT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.266304	119534	119653	1115-1265	1.000000	88.6
tRNA-Leu-tRNA-Asn_p4	tRNA-Leu-tRNA-Asn	4	TCGGTACTAGACGATGAGCA	CTGCCTTGGATGCCCATAAT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.326115	119534	119653	1311-1461	1.000000	87.6
tRNA-Leu-tRNA-Asn_p5	tRNA-Leu-tRNA-Asn	5	GGATTTCCACTTCCACCGAG	CCAGATAGTTATGCTCCCGC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.346988	119534	119653	1281-1335	0.600000	68.7