primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	rps19	1	TTCTGACCTCCGTACTTCGA	AGATATTGGGTATTTCTGTTACT	20	23	50.0	30.4	57.8	52.6	0.0	0.0	8.561888	54075	54356	430-430	0.500000	20.0
rps19_p2	rps19	2	TTCTGACCTCCGTACTTCGA	AAGATATTGGGTATTTCTGTTACT	20	24	50.0	29.2	57.8	53.5	0.0	0.0	8.725640	54075	54356	431-431	0.500000	20.0
rps19_p3	rps19	3	TTCTGACCTCCGTACTTCGA	AGATATTGGGTATTTCTGTTACTT	20	24	50.0	29.2	57.8	53.5	0.0	0.0	8.725640	54075	54356	430-430	0.500000	20.0
rps19_p4	rps19	4	TCTGACCTCCGTACTTCGAT	AGATATTGGGTATTTCTGTTACT	20	23	50.0	30.4	57.6	52.6	0.0	0.0	8.787287	54075	54356	429-429	0.500000	20.0
rps19_p5	rps19	5	CCTCCGTACTTCGATCGAGA	AGATATTGGGTATTTCTGTTACT	20	23	55.0	30.4	58.4	52.6	18.5	0.0	8.792595	54075	54356	424-424	0.500000	20.0
trnK-UUU_p1	trnK-UUU	1	CACAAGCTTGTACTTTCGCG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.1	58.0	30.7	0.0	0.099197	55789	58348	2898-2906	1.000000	80.3
trnK-UUU_p2	trnK-UUU	2	GACTCCCAAGCACACGTATT	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.131319	55789	58348	2827-2835	1.000000	80.0
trnK-UUU_p3	trnK-UUU	3	GACTCCCAAGCACACGTATT	TTTGGCCATCGTGATTAGGG	20	20	50.0	50.0	57.9	57.9	0.0	6.1	0.146097	55789	58348	2952-2960	1.000000	79.9
trnK-UUU_p4	trnK-UUU	4	TGCGGTCAATAAGGTAGGGA	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.167844	55789	58348	2989-2997	1.000000	79.7
trnK-UUU_p5	trnK-UUU	5	AAGCTTGTACTTTCGCGTCT	AATGTTTTGACAGAAGCGCG	20	20	45.0	45.0	57.8	58.0	5.8	0.0	0.186954	55789	58348	2895-2903	1.000000	79.6
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	21	20	47.6	45.0	58.3	58.0	0.0	0.0	1.340971	57630	58310	875-875	1.000000	69.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTGAAAGAGAAGTGGG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	57630	58310	875-875	1.000000	67.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	CTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	57630	58310	874-874	1.000000	67.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	57.9	58.0	0.0	0.0	2.164474	57630	58310	873-873	1.000000	63.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTGAAAGAGAAGTGGGTA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	58.1	58.0	0.0	0.0	2.178422	57630	58310	875-875	1.000000	62.8
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCGCGTCCAG	20	20	50.0	50.0	57.9	57.9	1.4	5.8	0.130641	61314	61718	549-554	1.000000	80.7
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	20	20	50.0	50.0	57.9	57.8	1.4	0.0	0.297375	61314	61718	527-532	1.000000	79.4
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCGCGTCCAG	20	20	45.0	50.0	58.3	57.9	1.4	5.8	0.383351	61314	61718	546-551	1.000000	78.7
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCGCGTCCAG	20	20	45.0	50.0	58.3	57.9	1.4	5.8	0.383351	61314	61718	547-552	1.000000	78.7
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCGCGTCCAG	20	20	45.0	50.0	58.3	57.9	1.4	5.8	0.383351	61314	61718	548-553	1.000000	78.7
trnS-CGA_p1	trnS-CGA	1	CCTCGTGAGCTACCAAACTG	GGCTCAGTGTCATGAACCTT	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.476333	67180	67936	978-987	1.000000	77.3
trnS-CGA_p2	trnS-CGA	2	CAAACTGCTCTACTCCGCTC	GGCTCAGTGTCATGAACCTT	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.556426	67180	67936	965-974	1.000000	76.7
trnS-CGA_p3	trnS-CGA	3	CCTCGTGAGCTACCAAACTG	AGCCTTCTTGTCAAATGGCT	20	20	55.0	45.0	58.3	57.7	0.0	15.7	0.584532	67180	67936	931-940	1.000000	76.5
trnS-CGA_p4	trnS-CGA	4	AAACTGCTCTACTCCGCTCT	GGCTCAGTGTCATGAACCTT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.639676	67180	67936	964-973	1.000000	76.0
trnS-CGA_p5	trnS-CGA	5	CAAACTGCTCTACTCCGCTC	AGCCTTCTTGTCAAATGGCT	20	20	55.0	45.0	58.4	57.7	0.0	15.7	0.664624	67180	67936	918-927	1.000000	75.9
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	72030	72519	598-1063	1.000000	71.4
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	72030	72519	597-1062	1.000000	71.4
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	72030	72519	539-1004	1.000000	71.2
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	72030	72519	600-1065	1.000000	70.9
petN-trnC-GCA_p5	petN-trnC-GCA	5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	72030	72519	599-1064	1.000000	70.9
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	72591	73749	1226-1228	1.000000	80.0
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	72591	73749	1233-1235	1.000000	78.9
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	72591	73749	1241-1243	1.000000	77.7
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	72591	73749	1240-1242	1.000000	77.7
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	20	20	50.0	45.0	58.1	57.6	14.6	13.0	0.442790	72591	73749	1305-1307	1.000000	77.3
trnR-UCU-rps14_p1	trnR-UCU-rps14	1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	21	20	47.6	50.0	56.9	58.0	0.0	6.1	2.074369	90239	90617	486-486	1.000000	64.7
trnR-UCU-rps14_p2	trnR-UCU-rps14	2	AGAAGACCTCTGTCCTATCCA	CATTTTGGGCTATCCGGACA	21	20	47.6	50.0	56.9	57.9	0.0	6.1	2.102939	90239	90617	496-496	1.000000	64.5
trnR-UCU-rps14_p3	trnR-UCU-rps14	3	AGAAGACCTCTGTCCTATCCA	TCGACATTTTGGGCTATCCG	21	20	47.6	50.0	56.9	58.1	0.0	0.0	2.103500	90239	90617	500-500	1.000000	64.5
trnR-UCU-rps14_p4	trnR-UCU-rps14	4	AGAAGACCTCTGTCCTATCCA	ACATTTTGGGCTATCCGGAC	21	20	47.6	50.0	56.9	57.9	0.0	6.1	2.104387	90239	90617	497-497	1.000000	64.5
trnR-UCU-rps14_p5	trnR-UCU-rps14	5	AGAAGACCTCTGTCCTATCCA	ATTTTGGGCTATCCGGACAC	21	20	47.6	50.0	56.9	57.9	0.0	6.1	2.104387	90239	90617	495-495	1.000000	64.5
psaA-ycf3_p1	psaA-ycf3	1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.091171	95559	96248	824-851	1.000000	82.1
psaA-ycf3_p2	psaA-ycf3	2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.132613	95559	96248	820-847	1.000000	81.8
psaA-ycf3_p3	psaA-ycf3	3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	95559	96248	822-849	1.000000	81.0
psaA-ycf3_p4	psaA-ycf3	4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	20	20	50.0	50.0	57.8	58.0	0.0	18.7	0.235180	95559	96248	823-850	1.000000	81.0
psaA-ycf3_p5	psaA-ycf3	5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.276621	95559	96248	818-845	1.000000	80.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	100228	100842	716-718	1.000000	81.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	100228	100842	708-710	1.000000	81.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	100228	100842	686-688	1.000000	81.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	100228	100842	706-708	1.000000	80.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	100228	100842	715-717	1.000000	79.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175907	101926	102506	716-727	1.000000	79.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	101926	102506	691-702	1.000000	79.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	101926	102506	681-692	1.000000	79.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	20	20	50.0	45.0	58.1	57.5	0.0	0.0	0.531462	101926	102506	728-739	1.000000	76.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.557071	101926	102506	719-730	1.000000	76.8
ndhK-ndhC_p1	ndhK-ndhC	1	ACCGTATAGAAGGGGCCATA	AAAGGAGCCTTGGAATGGTC	20	20	50.0	50.0	57.3	57.8	4.4	1.9	0.930264	103765	103814	194-194	1.000000	73.4
ndhK-ndhC_p2	ndhK-ndhC	2	ACCGTATAGAAGGGGCCATAA	AAAGGAGCCTTGGAATGGTC	21	20	47.6	50.0	58.0	57.8	4.4	1.9	1.241678	103765	103814	194-194	1.000000	70.9
ndhK-ndhC_p3	ndhK-ndhC	3	ACCGTATAGAAGGGGCCATA	AAGGAGCCTTGGAATGGTCT	20	20	50.0	50.0	57.3	58.6	4.4	0.0	1.331388	103765	103814	193-193	1.000000	70.2
ndhK-ndhC_p4	ndhK-ndhC	4	ACCGTATAGAAGGGGCCATA	AGGAGCCTTGGAATGGTCTT	20	20	50.0	50.0	57.3	58.6	4.4	0.0	1.331388	103765	103814	192-192	1.000000	70.2
ndhK-ndhC_p5	ndhK-ndhC	5	ACCGTATAGAAGGGGCCATA	AAAAGGAGCCTTGGAATGGT	20	20	50.0	45.0	57.3	57.3	4.4	1.9	1.410308	103765	103814	195-195	1.000000	69.5
rpl23-psaI_p1	rpl23-psaI	1	CCCTTCAACCGGGTTATTCT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.2	57.9	13.0	26.9	0.878489	110841	111902	1183-1183	0.500000	53.0
rpl23-psaI_p2	rpl23-psaI	2	AGGTAGAAGAATAGGGCCCA	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.1	57.9	27.6	26.9	0.966935	110841	111902	1242-1242	0.500000	52.3
rpl23-psaI_p3	rpl23-psaI	3	CCCTTCAACCGGGTTATTCT	GAAGTCATTGCAATTGCCGG	20	20	50.0	50.0	57.2	58.4	13.0	26.9	1.168654	110841	111902	1186-1186	0.500000	50.7
rpl23-psaI_p4	rpl23-psaI	4	AAGGTAGAAGAATAGGGCCCA	GTCATTGCAATTGCCGGAAA	21	20	47.6	45.0	57.9	57.9	27.6	26.9	1.222972	110841	111902	1243-1243	0.500000	50.2
rpl23-psaI_p5	rpl23-psaI	5	AGGTAGAAGAATAGGGCCCA	GAAGTCATTGCAATTGCCGG	20	20	50.0	50.0	57.1	58.4	27.6	26.9	1.257100	110841	111902	1245-1245	0.500000	49.9
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	116843	118010	875-1326	1.000000	80.7
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	116843	118010	873-1324	1.000000	80.7
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	116843	118010	864-1315	1.000000	80.4
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	116843	118010	862-1313	1.000000	80.4
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	116843	118010	866-1317	1.000000	79.0
psaJ-rpl33_p1	psaJ-rpl33	1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.863768	119298	119740	644-646	1.000000	74.0
psaJ-rpl33_p2	psaJ-rpl33	2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.895813	119298	119740	621-623	1.000000	73.7
psaJ-rpl33_p3	psaJ-rpl33	3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.975755	119298	119740	633-635	1.000000	73.1
psaJ-rpl33_p4	psaJ-rpl33	4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	20	20	55.0	55.0	58.9	58.1	0.0	0.0	1.025719	119298	119740	643-645	1.000000	72.7
psaJ-rpl33_p5	psaJ-rpl33	5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	20	20	55.0	50.0	58.9	58.2	0.0	0.0	1.057765	119298	119740	620-622	1.000000	72.4