primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGAAAGTGTTGTTGCCGAGA	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.7	58.2	0.0	17.1	0.494003	3271	4087	896-900	1.000000	76.9
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGAAAGTGTTGTTGCCGAGA	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.7	58.2	0.0	17.1	0.494003	3271	4087	895-899	1.000000	76.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGAAAGTGTTGTTGCCGAGA	GTCGTGCAGTGCAATTCAAT	20	20	45.0	45.0	57.7	57.7	0.0	21.7	0.662137	3271	4087	976-980	1.000000	75.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	AAGTGTTGTTGCCGAGATCT	AACGGTAGAGTACTCGGCTT	20	20	45.0	50.0	57.4	58.2	0.0	17.1	0.719250	3271	4087	893-897	1.000000	75.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	AAGTGTTGTTGCCGAGATCT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.4	58.2	0.0	17.1	0.719250	3271	4087	892-896	1.000000	75.1
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	28840	29267	552-584	1.000000	74.2
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	28840	29267	547-579	1.000000	73.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	28840	29267	497-529	1.000000	73.2
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	28840	29267	556-588	1.000000	71.2
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	28840	29267	555-587	1.000000	70.5
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	34589	34725	244-244	1.000000	81.5
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	34589	34725	243-243	1.000000	81.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.188901	34589	34725	283-283	1.000000	81.3
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	34589	34725	254-254	1.000000	80.8
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.259146	34589	34725	294-294	1.000000	80.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	8.5	0.0	0.269796	49894	51330	1575-1581	1.000000	78.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	8.5	0.0	0.280450	49894	51330	1567-1573	1.000000	78.4
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	58.1	58.5	8.5	0.0	0.546572	49894	51330	1657-1663	1.000000	76.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	8.5	0.0	0.557226	49894	51330	1649-1655	1.000000	76.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	8.5	0.0	0.613568	49894	51330	1574-1580	1.000000	75.7
rpl16_p1	rpl16	1	TCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	20	26	40.0	30.8	54.9	58.1	0.0	0.0	9.192583	81579	83169	1707-1738	1.000000	41.9
rpl16_p2	rpl16	2	TCAATGCAAAGGATGAAGGA	GGTTGAATAAAAATTCGATTGACCA	20	25	40.0	32.0	54.9	56.9	0.0	0.0	9.198462	81579	83169	1706-1737	1.000000	41.9
rpl16_p3	rpl16	3	TCAATGCAAAGGATGAAGGAA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	81579	83169	1707-1738	1.000000	41.9
rpl16_p4	rpl16	4	TTCAATGCAAAGGATGAAGGA	AGGTTGAATAAAAATTCGATTGACCA	21	26	38.1	30.8	55.7	58.1	0.0	0.0	9.360297	81579	83169	1708-1739	1.000000	41.9
rpl16_p5	rpl16	5	TCAATGCAAAGGATGAAGGAA	GGTTGAATAAAAATTCGATTGACCA	21	25	38.1	32.0	55.7	56.9	0.0	0.0	9.366175	81579	83169	1706-1737	1.000000	41.9
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.034958	94024	94933	1324-1325	1.000000	88.1
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.036329	94024	94933	1326-1327	1.000000	88.1
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	CAGCCCTTTGTCTCTGTGTT	TGCTAAAAAGCGTGGAGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.051468	94024	94933	1293-1294	1.000000	88.1
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	GGAACCATGCAGTACCAGAG	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.0	58.0	0.0	15.9	0.037669	94024	94933	1435-1435	0.333333	53.0
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	GGAACCATGCAGTACCAGAG	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.0	58.0	0.0	15.9	0.039039	94024	94933	1437-1437	0.333333	53.0
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TTGCGATTGCTCAGGATACC	20	20	50.0	50.0	58.0	58.0	5.8	6.5	0.054271	95015	95596	624-649	1.000000	90.8
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	GTCTCGCGTGTCTACCATTT	ACCCGATTATTGCGATTGCT	20	20	50.0	45.0	58.0	58.0	5.8	6.5	0.040512	95015	95596	658-658	0.333333	53.0
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	CTCGCGTGTCTACCATTTCA	ACCCGATTATTGCGATTGCT	20	20	50.0	45.0	58.0	58.0	5.8	6.5	0.041882	95015	95596	656-656	0.333333	53.0
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GTCTCGCGTGTCTACCATTT	TTATTCTGGTGTGGATGGCG	20	20	50.0	50.0	58.0	58.0	5.8	0.0	0.044788	95015	95596	896-896	0.333333	53.0
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	CTCGCGTGTCTACCATTTCA	TTATTCTGGTGTGGATGGCG	20	20	50.0	50.0	58.0	58.0	5.8	0.0	0.046158	95015	95596	894-894	0.333333	53.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTCTACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.630573	113742	114356	707-708	1.000000	60.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTCTACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	55.4	58.0	9.5	0.0	2.631944	113742	114356	708-709	1.000000	60.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTCTACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	55.4	57.9	9.5	0.0	2.747020	113742	114356	717-718	1.000000	59.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTCTACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	113742	114356	716-717	1.000000	59.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTCTACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	55.4	57.8	9.5	0.0	2.819038	113742	114356	715-716	1.000000	59.2
ccsA-ndhD_p1	ccsA-ndhD	1	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	21	20	42.9	50.0	57.5	57.5	29.8	0.0	1.998149	115482	115697	384-384	1.000000	65.1
ccsA-ndhD_p2	ccsA-ndhD	2	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	21	20	42.9	50.0	57.5	57.4	29.8	0.0	2.005202	115482	115697	383-383	1.000000	65.0
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCACTTATAGC	TCAGTTGACAGGGTACAAGC	20	20	45.0	50.0	56.0	57.5	29.8	0.0	2.509324	115482	115697	384-384	1.000000	61.0
ccsA-ndhD_p4	ccsA-ndhD	4	ACGAATTCGGCACTTATAGCT	ATCAGTTGACAGGGTACAAGC	21	21	42.9	47.6	57.5	57.9	29.8	0.0	2.513723	115482	115697	385-385	1.000000	60.9
ccsA-ndhD_p5	ccsA-ndhD	5	ACGAATTCGGCACTTATAGC	CAGTTGACAGGGTACAAGCT	20	20	45.0	50.0	56.0	57.4	29.8	0.0	2.516376	115482	115697	383-383	1.000000	60.9
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	117568	118213	800-839	1.000000	78.1
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	117568	118213	801-840	1.000000	78.0
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	117568	118213	821-860	1.000000	77.9
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	117568	118213	799-838	1.000000	76.9
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	117568	118213	776-815	1.000000	76.7
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TTGCGATTGCTCAGGATACC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	6.5	5.8	0.054271	144116	144697	624-649	1.000000	90.8
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	ACCCGATTATTGCGATTGCT	GTCTCGCGTGTCTACCATTT	20	20	45.0	50.0	58.0	58.0	6.5	5.8	0.040512	144116	144697	658-658	0.333333	53.0
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	ACCCGATTATTGCGATTGCT	CTCGCGTGTCTACCATTTCA	20	20	45.0	50.0	58.0	58.0	6.5	5.8	0.041882	144116	144697	656-656	0.333333	53.0
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TTATTCTGGTGTGGATGGCG	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.044788	144116	144697	896-896	0.333333	53.0
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TTATTCTGGTGTGGATGGCG	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.046158	144116	144697	894-894	0.333333	53.0
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	CAGCCCTTTGTCTCTGTGTT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.034958	144779	145688	1324-1325	1.000000	88.1
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	CAGCCCTTTGTCTCTGTGTT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.036329	144779	145688	1326-1327	1.000000	88.1
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGCTAAAAAGCGTGGAGGTT	CAGCCCTTTGTCTCTGTGTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.051468	144779	145688	1293-1294	1.000000	88.1
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	AAATGGTAGACACGCGAGAC	GGAACCATGCAGTACCAGAG	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.037669	144779	145688	1435-1435	0.333333	53.0
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	TGAAATGGTAGACACGCGAG	GGAACCATGCAGTACCAGAG	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.039039	144779	145688	1437-1437	0.333333	53.0
ycf2_p1	ycf2	1	AACACAGAGACAAAGGGCTG	TCGGTCTCTTGGTGAACTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030330	146431	146550	1261-1261	0.333333	53.1
ycf2_p2	ycf2	2	AACACAGAGACAAAGGGCTG	GTGCACGAGATCTTGTAGCA	20	20	50.0	50.0	58.0	58.0	0.0	24.5	0.030836	146431	146550	997-997	0.333333	53.1
ycf2_p3	ycf2	3	AACACAGAGACAAAGGGCTG	TGCACGAGATCTTGTAGCAC	20	20	50.0	50.0	58.0	58.0	0.0	24.5	0.030836	146431	146550	996-996	0.333333	53.1
ycf2_p4	ycf2	4	CTCTGGTACTGCATGGTTCC	TCGGTCTCTTGGTGAACTCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.033040	146431	146550	1150-1150	0.333333	53.1
ycf2_p5	ycf2	5	CTCTGGTACTGCATGGTTCC	TGCACGAGATCTTGTAGCAC	20	20	55.0	50.0	58.0	58.0	0.0	24.5	0.033547	146431	146550	885-885	0.333333	53.1