primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
chlB-trnQ-UUG_p1	chlB-trnQ-UUG	1	TCCTAGAGGAGCGTGCATAA	TGGTCCTGTTATTGCGAAGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.175315	7824	8015	336-337	1.000000	79.8
chlB-trnQ-UUG_p2	chlB-trnQ-UUG	2	TCCTAGAGGAGCGTGCATAA	GGTCCTGTTATTGCGAAGGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.176790	7824	8015	335-336	1.000000	79.8
chlB-trnQ-UUG_p3	chlB-trnQ-UUG	3	GAGCAGGACCAGCATACATC	TGGTCCTGTTATTGCGAAGG	20	20	55.0	50.0	58.1	57.9	11.9	0.0	0.231628	7824	8015	268-269	1.000000	79.6
chlB-trnQ-UUG_p4	chlB-trnQ-UUG	4	GAGCAGGACCAGCATACATC	GGTCCTGTTATTGCGAAGGT	20	20	55.0	50.0	58.1	57.9	11.9	0.0	0.233103	7824	8015	267-268	1.000000	79.6
chlB-trnQ-UUG_p5	chlB-trnQ-UUG	5	AGCTACTCGTAGGGTTCCAA	TGGTCCTGTTATTGCGAAGG	20	20	50.0	50.0	57.8	57.9	6.3	0.0	0.334912	7824	8015	291-292	1.000000	78.7
trnG-UCC_p1	trnG-UCC	1	CCAGGGTATTCATAGGAGAGGT	GGACAAAAGATCGATCTGTATGC	22	23	50.0	43.5	58.1	57.7	0.0	27.9	5.472740	43514	43536	177-177	1.000000	40.5
trnG-UCC_p2	trnG-UCC	2	ACCAGGGTATTCATAGGAGAGG	GGACAAAAGATCGATCTGTATGC	22	23	50.0	43.5	58.1	57.7	0.0	27.9	5.472740	43514	43536	178-178	1.000000	40.4
trnG-UCC_p3	trnG-UCC	3	ACCAGGGTATTCATAGGAGAGG	TGGACAAAAGATCGATCTGT	22	20	50.0	40.0	58.1	54.4	0.0	27.9	5.742373	43514	43536	179-179	1.000000	40.4
trnG-UCC_p4	trnG-UCC	4	CCAGGGTATTCATAGGAGAGGT	TGGACAAAAGATCGATCTGT	22	20	50.0	40.0	58.1	54.4	0.0	27.9	5.742373	43514	43536	178-178	1.000000	40.4
trnG-UCC_p5	trnG-UCC	5	CCAGGGTATTCATAGGAGAGGT	TTGGACAAAAGATCGATCTGT	22	21	50.0	38.1	58.1	55.2	0.0	27.9	5.894052	43514	43536	179-179	1.000000	40.4
rpl32_p1	rpl32	1	CACAAACCTCTTTGGGACGA	CGACTCTCGCCGTATGAAAG	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.570332	55052	55852	1796-1813	1.000000	84.3
rpl32_p2	rpl32	2	TTCCAAATTAACGTGGCCCA	CGACTCTCGCCGTATGAAAG	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.535327	55052	55852	1909-1926	1.000000	84.2
rpl32_p3	rpl32	3	TGCATTGATGGATGATGGGG	CGACTCTCGCCGTATGAAAG	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.536062	55052	55852	2078-2095	1.000000	83.6
rpl32_p4	rpl32	4	TCGTTTACGTTTACCCGCAA	CGACTCTCGCCGTATGAAAG	20	20	45.0	55.0	58.1	58.5	0.0	0.0	0.674034	55052	55852	1821-1838	1.000000	83.4
rpl32_p5	rpl32	5	ATCATGAGGTCCTTCTCCGT	CGACTCTCGCCGTATGAAAG	20	20	50.0	55.0	57.8	58.5	0.1	0.0	0.699032	55052	55852	2045-2062	1.000000	82.4
rpl32-trnM-CAU_p1	rpl32-trnM-CAU	1	ATCTTCGATGTCGCAGCAAT	ACCAGCTGTATCGCTATCCT	20	20	45.0	50.0	58.1	58.0	0.0	13.8	0.057118	55853	56383	1274-1276	1.000000	82.9
rpl32-trnM-CAU_p2	rpl32-trnM-CAU	2	GATAACGATCGGCAAGCTCA	ACCAGCTGTATCGCTATCCT	20	20	50.0	50.0	58.1	58.0	21.0	13.8	0.149564	55853	56383	1152-1154	1.000000	82.1
rpl32-trnM-CAU_p3	rpl32-trnM-CAU	3	ATCTTCGATGTCGCAGCAAT	CCGTCTGTAGCACTCATAGC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.181526	55853	56383	1357-1359	1.000000	81.7
rpl32-trnM-CAU_p4	rpl32-trnM-CAU	4	TCCCACTCCTCCCTTTCTTT	ACCAGCTGTATCGCTATCCT	20	20	50.0	50.0	57.9	58.0	0.0	13.8	0.136363	55853	56383	1458-1458	0.500000	58.9
rpl32-trnM-CAU_p5	rpl32-trnM-CAU	5	TTCCCACTCCTCCCTTTCTT	ACCAGCTGTATCGCTATCCT	20	20	50.0	50.0	57.9	58.0	0.0	13.8	0.136363	55853	56383	1459-1459	0.500000	58.9
trnM-CAU-atpB_p1	trnM-CAU-atpB	1	GCGAGAGTCGTTGGTTCAAA	CCTGCCGTATGCTCAAAAGA	20	20	50.0	50.0	58.8	58.3	0.0	0.0	1.047795	56456	56699	330-331	1.000000	74.3
trnM-CAU-atpB_p2	trnM-CAU-atpB	2	GCTTTCATACGGCGAGAGTC	CCTGCCGTATGCTCAAAAGA	20	20	55.0	50.0	58.8	58.3	16.6	0.0	1.060819	56456	56699	341-342	1.000000	74.1
trnM-CAU-atpB_p3	trnM-CAU-atpB	3	AGAGTATCGCTTTCATACGGC	CCTGCCGTATGCTCAAAAGA	21	20	47.6	50.0	58.0	58.3	0.0	0.0	1.276766	56456	56699	349-350	1.000000	72.3
trnM-CAU-atpB_p4	trnM-CAU-atpB	4	GCGAGAGTCGTTGGTTCAA	CCTGCCGTATGCTCAAAAGA	19	20	52.6	50.0	58.1	58.3	0.0	0.0	1.367123	56456	56699	330-331	1.000000	71.7
trnM-CAU-atpB_p5	trnM-CAU-atpB	5	GCGAGAGTCGTTGGTTCAAA	ATCTGAGTAATACGGCCTGC	20	20	50.0	50.0	58.8	57.2	0.0	0.0	1.607623	56456	56699	345-346	1.000000	69.7
ycf1_p1	ycf1	1	CTCACTCGGTCTTCAAGCTC	TTCTGTTCACGTGTGGAAGG	20	20	55.0	50.0	58.0	58.1	0.0	16.5	0.062465	73659	73778	734-761	1.000000	84.9
ycf1_p2	ycf1	2	CTCTCACTCGGTCTTCAAGC	TTCTGTTCACGTGTGGAAGG	20	20	55.0	50.0	58.0	58.1	0.0	16.5	0.062465	73659	73778	736-763	1.000000	84.8
ycf1_p3	ycf1	3	GCTCTCACTCGGTCTTCAAG	TTCTGTTCACGTGTGGAAGG	20	20	55.0	50.0	58.0	58.1	0.0	16.5	0.062465	73659	73778	737-764	1.000000	84.8
ycf1_p4	ycf1	4	CTCACTCGGTCTTCAAGCTC	AGTCTTCCTTCAGGGTCCTC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.064990	73659	73778	579-597	1.000000	84.7
ycf1_p5	ycf1	5	CTCACTCGGTCTTCAAGCTC	GTCTTCCTTCAGGGTCCTCT	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.064990	73659	73778	578-596	1.000000	84.7
ycf1-rps12_p1	ycf1-rps12	1	ACATCTGTTTAGCCAAGGCA	CCTTTACGCTGAGGACATCC	20	20	45.0	55.0	57.4	58.1	14.4	0.0	0.643510	74573	75626	1131-1188	1.000000	80.6
ycf1-rps12_p2	ycf1-rps12	2	ACATCTGTTTAGCCAAGGCA	GCACATACACCCTAGCACAT	20	20	45.0	50.0	57.4	57.7	14.4	0.0	0.916898	74573	75626	1154-1211	1.000000	78.4
ycf1-rps12_p3	ycf1-rps12	3	ACATCTGTTTAGCCAAGGCA	TTTCTATTGGCTGTCTCGCG	20	20	45.0	50.0	57.4	58.4	14.4	0.0	0.944408	74573	75626	1085-1142	1.000000	78.2
ycf1-rps12_p4	ycf1-rps12	4	ACATCTGTTTAGCCAAGGCA	ACTCCTTTACGCTGAGGACA	20	20	45.0	50.0	57.4	58.4	14.4	0.0	0.955793	74573	75626	1134-1191	1.000000	78.1
ycf1-rps12_p5	ycf1-rps12	5	ACATCTGTTTAGCCAAGGCA	GCTGTCTCGCGTTTCTAGTA	20	20	45.0	50.0	57.4	57.5	14.4	5.8	1.124181	74573	75626	1076-1133	1.000000	76.8
infA_p1	infA	1	ATTTTGCGAACAGAAGCACG	AAACAGTTCGAGTAGCTGCT	20	20	45.0	45.0	58.0	57.2	0.0	3.1	0.858547	92920	93228	904-1040	1.000000	81.3
infA_p2	infA	2	ACGAATCAGACGGCACTTTT	AAACAGTTCGAGTAGCTGCT	20	20	45.0	45.0	58.1	57.2	0.0	3.1	0.943037	92920	93228	929-1065	1.000000	80.5
infA_p3	infA	3	GCACCTTTGAATCCACAAGC	AAACAGTTCGAGTAGCTGCT	20	20	50.0	45.0	57.9	57.2	0.0	3.1	0.892252	92920	93228	1333-1450	1.000000	79.8
infA_p4	infA	4	GTTTGCCCTCGTGTATCTGT	AAACAGTTCGAGTAGCTGCT	20	20	50.0	45.0	57.9	57.2	0.0	3.1	0.916753	92920	93228	1291-1408	1.000000	79.7
infA_p5	infA	5	TTGCGAAAACTTGCTCGAAC	AAACAGTTCGAGTAGCTGCT	20	20	45.0	45.0	57.9	57.2	0.0	3.1	0.921624	92920	93228	1255-1372	1.000000	79.7
infA-rps8_p1	infA-rps8	1	GACTAGAAACGCTCCACCAG	AGCAGCACCTAATATGCCTC	20	20	55.0	50.0	58.0	57.4	0.0	0.0	0.560250	93229	93572	1049-1185	1.000000	83.0
infA-rps8_p2	infA-rps8	2	GACTAGAAACGCTCCACCAG	GCAGCACCTAATATGCCTCT	20	20	55.0	50.0	58.0	57.4	0.0	0.0	0.560250	93229	93572	1048-1184	1.000000	83.0
infA-rps8_p3	infA-rps8	3	GGAAATCCTGCGATGACTGT	AGCAGCACCTAATATGCCTC	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.587758	93229	93572	1030-1166	1.000000	82.7
infA-rps8_p4	infA-rps8	4	GGAAATCCTGCGATGACTGT	GCAGCACCTAATATGCCTCT	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.587758	93229	93572	1029-1165	1.000000	82.7
infA-rps8_p5	infA-rps8	5	ATTTTGCGAACAGAAGCACG	GCAGCACCTAATATGCCTCT	20	20	45.0	50.0	58.0	57.4	0.0	0.0	0.594476	93229	93572	1301-1437	1.000000	82.3
rpl22-rps19_p1	rpl22-rps19	1	AACCACGAAGTTGATGAGCT	TTCCAGGGACATGCGAAAAA	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.562537	97143	97195	219-224	1.000000	78.0
rpl22-rps19_p2	rpl22-rps19	2	AACCACGAAGTTGATGAGCT	TTTCCAGGGACATGCGAAAA	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.562537	97143	97195	220-225	1.000000	78.0
rpl22-rps19_p3	rpl22-rps19	3	AACCACGAAGTTGATGAGCT	TTTTCCAGGGACATGCGAAA	20	20	45.0	45.0	57.4	58.0	0.0	0.0	0.562537	97143	97195	221-226	1.000000	78.0
rpl22-rps19_p4	rpl22-rps19	4	AACCACGAAGTTGATGAGCT	TCCATTTTCCAGGGACATGC	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.699526	97143	97195	225-230	1.000000	76.9
rpl22-rps19_p5	rpl22-rps19	5	AACCACGAAGTTGATGAGCT	ATTTTCCAGGGACATGCGAA	20	20	45.0	45.0	57.4	57.8	0.0	0.0	0.765206	97143	97195	222-227	1.000000	76.4
trnV-GAC-LK118_pgr004_p1	trnV-GAC-LK118_pgr004	1	ACCTTGACGTGGTGAAAGTC	TCCCAAGGGCAGGTTACTTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098304	113791	114640	979-1000	1.000000	83.1
trnV-GAC-LK118_pgr004_p2	trnV-GAC-LK118_pgr004	2	CCTTGACGTGGTGAAAGTCA	TCCCAAGGGCAGGTTACTTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099665	113791	114640	978-999	1.000000	83.1
trnV-GAC-LK118_pgr004_p3	trnV-GAC-LK118_pgr004	3	ACCTTGACGTGGTGAAAGTC	CCCAAGGGCAGGTTACTTAC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.174812	113791	114640	978-999	1.000000	82.5
trnV-GAC-LK118_pgr004_p4	trnV-GAC-LK118_pgr004	4	CCTTGACGTGGTGAAAGTCA	CCCAAGGGCAGGTTACTTAC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.176173	113791	114640	977-998	1.000000	82.5
trnV-GAC-LK118_pgr004_p5	trnV-GAC-LK118_pgr004	5	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTACTTACGCATT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.304065	113791	114640	973-994	1.000000	81.5
LK118_pgr004-trnI-GAU_p1	LK118_pgr004-trnI-GAU	1	GACCGGAGTGAAGTCGTAAC	GCAACGACGCAATTATCAGG	20	20	55.0	50.0	58.0	57.9	9.5	0.0	0.116665	116124	116553	540-545	1.000000	81.1
LK118_pgr004-trnI-GAU_p2	LK118_pgr004-trnI-GAU	2	GACCGGAGTGAAGTCGTAAC	CGCAACGACGCAATTATCAG	20	20	55.0	50.0	58.0	58.3	9.5	0.0	0.307010	116124	116553	541-546	1.000000	79.6
LK118_pgr004-trnI-GAU_p3	LK118_pgr004-trnI-GAU	3	GACCGGAGTGAAGTCGTAAC	AACGACGCAATTATCAGGGG	20	20	55.0	50.0	58.0	58.3	9.5	0.0	0.361819	116124	116553	538-543	1.000000	79.2
LK118_pgr004-trnI-GAU_p4	LK118_pgr004-trnI-GAU	4	CAAGGTAGCCGTACTGGAAG	GCAACGACGCAATTATCAGG	20	20	55.0	50.0	57.7	57.9	7.8	0.0	0.386405	116124	116553	521-526	1.000000	79.0
LK118_pgr004-trnI-GAU_p5	LK118_pgr004-trnI-GAU	5	AAGTCGTAACAAGGTAGCCG	GCAACGACGCAATTATCAGG	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.450091	116124	116553	530-535	1.000000	78.5
LK118_pgr003-LK118_pgr002_p1	LK118_pgr003-LK118_pgr002	1	CCACCTCAAGATGAGTGCTC	TCACTGCACTTCCACTTGAC	20	20	55.0	50.0	58.0	58.0	6.6	0.0	0.067796	121427	121428	81-81	1.000000	80.4
LK118_pgr003-LK118_pgr002_p2	LK118_pgr003-LK118_pgr002	2	GAAGCCCACCTCAAGATGAG	TCACTGCACTTCCACTTGAC	20	20	55.0	50.0	58.0	58.0	0.3	0.0	0.081971	121427	121428	86-86	1.000000	80.3
LK118_pgr003-LK118_pgr002_p3	LK118_pgr003-LK118_pgr002	3	GCGGATAATTGCTGAATGCC	TCACTGCACTTCCACTTGAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.102535	121427	121428	115-115	1.000000	79.9
LK118_pgr003-LK118_pgr002_p4	LK118_pgr003-LK118_pgr002	4	GTACCAGTTATCGTGCCCTC	TCACTGCACTTCCACTTGAC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.266920	121427	121428	162-162	1.000000	78.4
LK118_pgr003-LK118_pgr002_p5	LK118_pgr003-LK118_pgr002	5	CCACCTCAAGATGAGTGCTC	CACTGCACTTCCACTTGACA	20	20	55.0	50.0	58.0	58.3	6.6	0.0	0.354119	121427	121428	80-80	1.000000	78.2
ndhJ-chlN_p1	ndhJ-chlN	1	GTTGGATTGGGTGGCCTTTA	ACTTGGGTCCAACCACTTTG	20	20	50.0	50.0	58.1	58.2	3.3	0.0	0.292980	130009	130746	813-841	1.000000	81.7
ndhJ-chlN_p2	ndhJ-chlN	2	TTGGATTGGGTGGCCTTTAC	ACTTGGGTCCAACCACTTTG	20	20	50.0	50.0	58.1	58.2	3.3	0.0	0.292980	130009	130746	812-840	1.000000	81.7
ndhJ-chlN_p3	ndhJ-chlN	3	GTTGGATTGGGTGGCCTTTA	ATTCATGGGTTTGCCAATGC	20	20	50.0	45.0	58.1	57.6	3.3	0.0	0.474961	130009	130746	889-917	1.000000	79.9
ndhJ-chlN_p4	ndhJ-chlN	4	TTGGATTGGGTGGCCTTTAC	ATTCATGGGTTTGCCAATGC	20	20	50.0	45.0	58.1	57.6	3.3	0.0	0.474961	130009	130746	888-916	1.000000	79.9
ndhJ-chlN_p5	ndhJ-chlN	5	TTGGATTGGGTGGCCTTTAC	CCAGATTCATGGGTTTGCCA	20	20	50.0	50.0	58.1	58.4	3.3	0.0	0.508997	130009	130746	892-920	1.000000	79.6