primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	AGTCCTGTTGGCGAGATCTA	TCGATTCGCCTTCACGATTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.149980	3554	4268	853-894	0.909091	86.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	GAAGTCCTGTTGGCGAGATC	TCGATTCGCCTTCACGATTT	20	20	55.0	45.0	58.4	58.0	0.0	0.0	0.358393	3554	4268	855-896	0.924242	85.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	AGTCCTGTTGGCGAGATCTA	ATCGATTCGCCTTCACGATT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.370465	3554	4268	854-895	0.893939	84.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	GAAGTCCTGTTGGCGAGATC	ATCGATTCGCCTTCACGATT	20	20	55.0	45.0	58.4	57.8	0.0	0.0	0.578879	3554	4268	856-897	0.909091	83.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGTCCTGTTGGCGAGATCTA	AATCGATTCGCCTTCACGAT	20	20	50.0	45.0	57.9	57.8	0.0	4.4	0.370465	3554	4268	855-896	0.878788	82.7
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	TGAGCTATCCCGACCATTCT	CGAAGCCATACCCAATCGAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.140000	13871	14914	2082-2527	0.969697	89.7
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TGAGCTATCCCGACCATTCT	TTGACCCTTTCGCTTTTCCA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.148155	13871	14914	2011-2456	0.969697	89.6
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	TGAGCTATCCCGACCATTCT	AGGTCCTCTGTAACGTGACA	20	20	50.0	50.0	57.9	58.0	0.0	5.4	0.096983	13871	14914	1797-2242	0.924242	88.2
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	GCTTCCATTGAGTCTCTGCA	AGGTCCTCTGTAACGTGACA	20	20	50.0	50.0	57.9	58.0	0.6	5.4	0.113885	13871	14914	1320-1709	0.924242	88.1
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	CTCAGGATTGCCCATTGTGA	AGGTCCTCTGTAACGTGACA	20	20	50.0	50.0	57.9	58.0	0.0	5.4	0.142160	13871	14914	1265-1620	0.893939	86.6
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CACAAAAACGAAACGGTCCC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	28027	29092	827-1227	0.969697	87.2
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CCACAAAAACGAAACGGTCC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	28027	29092	828-1228	0.969697	87.2
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CCCACAAAAACGAAACGGTC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	28027	29092	829-1229	0.969697	87.2
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	ACAAAAACGAAACGGTCCCT	GGCGTAAGTCATCGGTTCAA	20	20	45.0	50.0	58.2	58.3	0.0	0.0	0.528876	28027	29092	826-1226	0.969697	86.6
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	AACCCACAAAAACGAAACGG	GGCGTAAGTCATCGGTTCAA	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.563947	28027	29092	831-1231	0.969697	86.3
petN-trnC-GCA_p1	petN-trnC-GCA	1	TTAAAGCAGCCCAAGCAAGA	CGATATGGCCGAGTGGTAAG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.072037	31781	32736	681-1082	0.969697	90.2
petN-trnC-GCA_p2	petN-trnC-GCA	2	CAGCCCAAGCAAGACTTACT	CGATATGGCCGAGTGGTAAG	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.257120	31781	32736	675-1076	0.969697	88.7
petN-trnC-GCA_p3	petN-trnC-GCA	3	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.088172	31781	32736	80-1042	0.909091	87.7
petN-trnC-GCA_p4	petN-trnC-GCA	4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.273256	31781	32736	80-1036	0.909091	86.2
petN-trnC-GCA_p5	petN-trnC-GCA	5	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.0	58.4	0.0	14.7	0.361535	31781	32736	687-1060	0.909091	85.5
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CTTACCACTCGGCCATATCG	TTCATTTCCAACCCCAAGCA	20	20	55.0	45.0	57.9	57.9	2.2	0.0	0.160093	32808	33958	1041-1273	0.939394	88.3
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.176228	32808	33958	1081-1313	0.909091	87.0
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	CTTACCACTCGGCCATATCG	TCATTTCCAACCCCAAGCAT	20	20	55.0	45.0	57.9	57.7	2.2	0.0	0.389991	32808	33958	1040-1272	0.939394	86.5
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	ATTCTCTTGTCGATCAGGCG	TTCATTTCCAACCCCAAGCA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.310007	32808	33958	1105-1337	0.893939	85.3
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	CCGGATTTGAACTGGGGAAA	TCATTTCCAACCCCAAGCAT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.406127	32808	33958	1080-1312	0.909091	85.1
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	GTCCTTAGAACGAATCGCACT	CAATCCGACGCTTTAGTCCA	21	20	47.6	50.0	58.1	58.0	1.2	0.0	1.127248	51810	52457	520-865	0.969697	81.8
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	GTCCTTAGAACGAATCGCACT	AATCCGACGCTTTAGTCCAC	21	20	47.6	50.0	58.1	58.0	1.2	0.0	1.128636	51810	52457	519-864	0.969697	81.8
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	ACGAATCGCACTTTTACCACT	CAATCCGACGCTTTAGTCCA	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.241837	51810	52457	511-856	0.969697	80.9
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	ACGAATCGCACTTTTACCACT	AATCCGACGCTTTAGTCCAC	21	20	42.9	50.0	58.2	58.0	1.2	0.0	1.243225	51810	52457	510-855	0.969697	80.8
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	ACGAATCGCACTTTTACCAC	CAATCCGACGCTTTAGTCCA	20	20	45.0	50.0	56.8	58.0	1.2	0.0	1.248863	51810	52457	511-856	0.969697	80.8
accD_p1	accD	1	ATGGGTGGTATGTTGCTGTC	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.9	57.9	0.0	26.9	0.206183	55134	56636	80-2932	0.757576	80.7
accD_p2	accD	2	GGGTGGTATGTTGCTGTCAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.9	57.9	0.0	26.9	0.206183	55134	56636	80-2930	0.757576	80.7
accD_p3	accD	3	GGGTGGTATGTTGCTGTCAT	AGGCACAAAAACGGAAGGTA	20	20	50.0	45.0	57.9	57.6	0.0	0.0	0.480476	55134	56636	80-2895	0.742424	77.9
accD_p4	accD	4	AGGGGAGGGATTGATCATGT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.8	57.9	0.0	26.9	0.296629	55134	56636	80-2969	0.393939	65.4
accD_p5	accD	5	GGGATAGGGGAGGGATTGAT	GTCATTGCAATTGCCGGAAA	20	20	55.0	45.0	57.6	57.9	0.0	26.9	0.445360	55134	56636	80-2974	0.409091	64.8
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	TGGTTCGTATTCCGGATTGG	21	20	42.9	50.0	57.2	57.7	5.1	4.1	2.081544	60229	61114	80-988	0.939394	72.9
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	GGTTCGTATTCCGGATTGGG	21	20	42.9	55.0	57.2	58.4	5.1	4.1	2.172605	60229	61114	80-987	0.939394	72.2
petA-psbJ_p3	petA-psbJ	3	GCGTCTATTATTTTGGCCCA	TGGTTCGTATTCCGGATTGG	20	20	45.0	50.0	56.2	57.7	0.0	4.1	2.142330	60229	61114	80-1038	0.924242	71.8
petA-psbJ_p4	petA-psbJ	4	GCGTCTATTATTTTGGCCCA	GGTTCGTATTCCGGATTGGG	20	20	45.0	55.0	56.2	58.4	0.0	4.1	2.233391	60229	61114	80-1037	0.924242	71.1
petA-psbJ_p5	petA-psbJ	5	TGAGAAGGTTCAATTGTCCGA	GTTCGTATTCCGGATTGGGA	21	20	42.9	50.0	57.2	57.4	5.1	4.1	2.370038	60229	61114	80-986	0.939394	70.6
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	CGAAATAACTGGTTATAGTGGGC	20	23	45.0	43.5	58.0	57.3	0.0	6.4	3.685898	61892	63023	80-1320	0.954545	60.7
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	CGAAATAACTGGTTATAGTGGGC	20	23	50.0	43.5	57.9	57.3	6.4	6.4	3.719892	61892	63023	80-1309	0.954545	60.4
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	CGAAATAACTGGTTATAGTGGGC	20	23	50.0	43.5	57.8	57.3	24.9	6.4	3.824901	61892	63023	80-1232	0.954545	59.6
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	CGAAATAACTGGTTATAGTGGGC	20	23	55.0	43.5	58.2	57.3	0.9	6.4	3.866489	61892	63023	80-1227	0.954545	59.2
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	CGAAATAACTGGTTATAGTGGGC	20	23	50.0	43.5	58.2	57.3	0.0	6.4	3.905851	61892	63023	80-1311	0.954545	58.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	57.9	57.8	5.7	11.2	0.351995	63887	64444	80-722	0.939394	86.8
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	58.2	57.8	5.7	11.2	0.409253	63887	64444	80-723	0.939394	86.3
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTGTACCCAAAACAAACGCG	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	57.8	57.8	3.5	11.2	0.428409	63887	64444	80-730	0.939394	86.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAAACAAACGCGCTACCAAG	ACCGCGAACCATAGAGTACT	20	20	45.0	50.0	57.9	58.2	5.7	8.2	0.353719	63887	64444	80-746	0.924242	86.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CAAAACAAACGCGCTACCAA	ACCGCGAACCATAGAGTACT	20	20	45.0	50.0	58.2	58.2	5.7	8.2	0.410977	63887	64444	80-747	0.924242	85.7
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.8	57.6	18.9	0.0	0.589447	102905	103391	80-623	0.969697	86.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	102905	103391	80-661	0.909091	84.2
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	GTTCCTCAGTAGCTCAGTGG	20	20	55.0	55.0	57.8	57.3	18.9	0.0	0.879733	102905	103391	80-662	0.909091	81.3
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CCTCAGTAGCTCAGTGGTAGA	20	21	55.0	52.4	57.8	58.0	18.9	0.0	1.222019	102905	103391	80-659	0.969697	81.0
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GGCCTGTAGCTCAGAGGATT	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	58.9	57.7	12.9	0.0	1.183436	102905	103391	80-672	0.909091	78.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GCCTAGTGGACCCTAAGCTA	CTGTCCCGTGATAAACCCAG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.107804	103464	104079	80-1911	0.909091	87.5
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GAGAGAGAAGCGCTTTCACA	CTGTCCCGTGATAAACCCAG	20	20	50.0	55.0	57.9	58.0	25.8	0.0	0.095864	103464	104079	80-2146	0.621212	76.1
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	TGCTCGTGGTAATTGTAGGC	CTGTCCCGTGATAAACCCAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.042718	103464	104079	80-1790	0.500000	71.7
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GTAAGGTAATCCGGGGGAGA	CTGTCCCGTGATAAACCCAG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.116774	103464	104079	80-1465	0.151515	57.1
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	TAAGGTAATCCGGGGGAGAC	CTGTCCCGTGATAAACCCAG	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.116774	103464	104079	80-1464	0.151515	57.1
ycf1_p1	ycf1	1	TGGATGCTTTCGTGGACAAA	AAATCCGGTTCGCGTGAATA	20	20	45.0	45.0	58.0	58.0	0.0	15.5	0.038672	105858	105977	80-909	0.590909	75.3
ycf1_p2	ycf1	2	TGGATGCTTTCGTGGACAAA	TCCGGTTCGCGTGAATAATT	20	20	45.0	45.0	58.0	58.0	0.0	15.5	0.038672	105858	105977	80-906	0.530303	72.9
ycf1_p3	ycf1	3	TGGATGCTTTCGTGGACAAA	AATCCGGTTCGCGTGAATAA	20	20	45.0	45.0	58.0	58.0	0.0	15.5	0.038672	105858	105977	80-908	0.515152	72.3
ycf1_p4	ycf1	4	AACTGACGGAAGGATCCTCA	AATCCGGTTCGCGTGAATAA	20	20	50.0	45.0	58.1	58.0	16.5	15.5	0.074352	105858	105977	80-213	0.121212	56.3
ycf1_p5	ycf1	5	AACTGACGGAAGGATCCTCA	TCCGGTTCGCGTGAATAATT	20	20	50.0	45.0	58.1	58.0	16.5	15.5	0.074352	105858	105977	80-211	0.121212	56.3
rpl32-ndhF_p1	rpl32-ndhF	1	AAGGCTTTCAATGCTGTCCA	AGGGCGCAGAAAGTAACAAA	20	20	45.0	45.0	58.0	58.0	0.0	10.8	0.024692	120042	120785	80-1592	0.818182	84.5
rpl32-ndhF_p2	rpl32-ndhF	2	AGGCTTTCAATGCTGTCCAA	AGGGCGCAGAAAGTAACAAA	20	20	45.0	45.0	58.0	58.0	0.0	10.8	0.024692	120042	120785	80-1591	0.818182	84.5
rpl32-ndhF_p3	rpl32-ndhF	3	AGGCTTTCAATGCTGTCCAA	TTTTTGACAAGCCTTTGCCG	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.067105	120042	120785	80-1430	0.818182	84.2
rpl32-ndhF_p4	rpl32-ndhF	4	AAGGCTTTCAATGCTGTCCA	TGTTGCCGTGGGAAAAAGTA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.054584	120042	120785	80-999	0.727273	80.7
rpl32-ndhF_p5	rpl32-ndhF	5	AGGCTTTCAATGCTGTCCAA	TGTTGCCGTGGGAAAAAGTA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.054584	120042	120785	80-998	0.727273	80.7