primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-rps16_p1 trnK-rps16 1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 20 20 50.0 45.0 58.2 58.0 20.3 7.6 0.192760 4055 4751 797-811 1.000000 82.6 trnK-rps16_p2 trnK-rps16 2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 20 20 50.0 45.0 58.2 58.0 20.3 7.6 0.192760 4055 4751 796-810 1.000000 82.6 trnK-rps16_p3 trnK-rps16 3 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 20 20 50.0 50.0 58.2 58.2 20.3 7.6 0.397463 4055 4751 801-815 1.000000 81.0 trnK-rps16_p4 trnK-rps16 4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 20 20 50.0 50.0 58.2 58.2 20.3 7.6 0.397463 4055 4751 801-815 1.000000 81.0 trnK-rps16_p5 trnK-rps16 5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 20 20 50.0 50.0 58.2 58.2 20.3 7.6 0.397463 4055 4751 800-814 1.000000 81.0 rps16-trnQ_p1 rps16-trnQ 1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.104521 5840 7518 1770-1777 1.000000 81.4 rps16-trnQ_p2 rps16-trnQ 2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.105939 5840 7518 1768-1775 1.000000 81.4 rps16-trnQ_p3 rps16-trnQ 3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.117059 5840 7518 1776-1783 1.000000 81.3 rps16-trnQ_p4 rps16-trnQ 4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.117059 5840 7518 1777-1784 1.000000 81.3 rps16-trnQ_p5 rps16-trnQ 5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 5840 7518 1752-1759 1.000000 80.5 trnS-trnG_p1 trnS-trnG 1 AATCTGCCGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 20 20 50.0 45.0 58.3 56.4 0.0 0.0 1.897349 8861 9717 940-963 1.000000 68.8 trnS-trnG_p2 trnS-trnG 2 CAATCTGCCGCTTTAGTCCA AACCGAAAGACCCCTTAACT 20 20 50.0 45.0 58.3 56.4 0.0 0.0 1.898577 8861 9717 941-964 1.000000 68.8 trnS-trnG_p3 trnS-trnG 3 CGGAGAGAGAGGGATTCGAA AACCGAAAGACCCCTTAACT 20 20 55.0 45.0 58.3 56.4 0.0 0.0 1.954878 8861 9717 994-1017 1.000000 68.1 trnS-trnG_p4 trnS-trnG 4 GCTTTAGTCCACTCAGCCAT AACCGAAAGACCCCTTAACT 20 20 50.0 45.0 57.6 56.4 0.9 0.0 2.046364 8861 9717 932-955 1.000000 67.6 trnS-trnG_p5 trnS-trnG 5 AATCTGCCGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 20 21 50.0 42.9 58.3 57.1 0.0 0.0 2.131471 8861 9717 941-964 1.000000 66.9 trnR-atpA_p1 trnR-atpA 1 GGAATGAAAAGCGTCCATTGT TGCTACCAAGACATTCACCG 21 20 42.9 50.0 57.4 57.9 5.3 0.0 1.669272 10685 10803 294-295 1.000000 68.3 trnR-atpA_p2 trnR-atpA 2 GGTTCAAATCCTATTGGACGC TGCTACCAAGACATTCACCG 21 20 47.6 50.0 57.3 57.9 0.0 0.0 1.786097 10685 10803 234-235 1.000000 67.1 trnR-atpA_p3 trnR-atpA 3 GGAATGAAAAGCGTCCATTGT GCTACCAAGACATTCACCGA 21 20 42.9 50.0 57.4 57.6 5.3 0.0 1.956049 10685 10803 293-294 1.000000 66.0 trnR-atpA_p4 trnR-atpA 4 GGAATGAAAAGCGTCCATTGT TACCAAGACATTCACCGAGC 21 20 42.9 50.0 57.4 57.6 5.3 0.0 1.956049 10685 10803 291-292 1.000000 66.0 trnR-atpA_p5 trnR-atpA 5 AGGAATGAAAAGCGTCCATTG TGCTACCAAGACATTCACCG 21 20 42.9 50.0 57.1 57.9 5.3 0.0 1.948995 10685 10803 296-296 0.500000 44.4 trnD-trnY_p1 trnD-trnY 1 GTGCTCTGACCGATTGAACT CTGGTTCAAATCCAGCTCGG 20 20 50.0 55.0 57.9 58.9 0.0 0.0 0.996135 31960 32253 337-346 1.000000 76.7 trnD-trnY_p2 trnD-trnY 2 GGATCCTAGTTCGGGACTGA CTGGTTCAAATCCAGCTCGG 20 20 55.0 55.0 57.9 58.9 3.8 0.0 0.978915 31960 32253 394-403 1.000000 76.2 trnD-trnY_p3 trnD-trnY 3 GTGCTCTGACCGATTGAACT TCTACGCTGGTTCAAATCCA 20 20 50.0 45.0 57.9 56.9 0.0 0.0 1.227353 31960 32253 343-352 1.000000 74.8 trnD-trnY_p4 trnD-trnY 4 GGATCCTAGTTCGGGACTGA TCTACGCTGGTTCAAATCCA 20 20 55.0 45.0 57.9 56.9 3.8 0.0 1.210133 31960 32253 400-409 1.000000 74.3 trnD-trnY_p5 trnD-trnY 5 ATCGGATCCTAGTTCGGGAC CTGGTTCAAATCCAGCTCGG 20 20 55.0 55.0 58.4 58.9 10.8 0.0 1.291593 31960 32253 397-406 1.000000 73.7 psbC-trnS_p1 psbC-trnS 1 TCGATCGTGATTTGGAACCC GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.0 58.0 15.9 0.0 0.039897 36815 36951 276-276 1.000000 82.6 psbC-trnS_p2 psbC-trnS 2 TCGATCGTGATTTGGAACCC GGCTGAGTGGTTGATAGCTC 20 20 50.0 55.0 58.0 58.1 15.9 0.0 0.071797 36815 36951 269-269 1.000000 82.4 psbC-trnS_p3 psbC-trnS 3 AACCCGTTCTTTCCATGACC GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.115718 36815 36951 261-261 1.000000 82.1 psbC-trnS_p4 psbC-trnS 4 TGGAACCCGTTCTTTCCATG GAGAGATGGCTGAGTGGTTG 20 20 50.0 55.0 58.1 58.0 7.5 0.0 0.117068 36815 36951 264-264 1.000000 82.1 psbC-trnS_p5 psbC-trnS 5 TCGATCGTGATTTGGAACCC AGCTCTGGTCTTGAAAACCG 20 20 50.0 50.0 58.0 58.1 15.9 0.0 0.134307 36815 36951 254-254 1.000000 82.1 trnT-trnL_p1 trnT-trnL 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 47889 48847 1009-1013 1.000000 83.1 trnT-trnL_p2 trnT-trnL 2 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.140570 47889 48847 1030-1034 1.000000 83.0 trnT-trnL_p3 trnT-trnL 3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 47889 48847 1017-1021 1.000000 82.5 trnT-trnL_p4 trnT-trnL 4 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 47889 48847 1041-1045 1.000000 82.1 trnT-trnL_p5 trnT-trnL 5 GGAATCGAACCGATGACCAT ACAATCAAGTCCGTAGCGTC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.248324 47889 48847 1062-1066 1.000000 82.0 ndhJ-ndhK_p1 ndhJ-ndhK 1 GCAATCGAAGCCCAAAGATC CAGACAGAATTCTTTTGTGCAGA 20 23 50.0 39.1 57.5 57.6 2.1 4.9 3.910398 51085 51165 177-177 1.000000 49.6 ndhJ-ndhK_p2 ndhJ-ndhK 2 GCAATCGAAGCCCAAAGATC TCAGACAGAATTCTTTTGTGCAG 20 23 50.0 39.1 57.5 57.6 2.1 4.9 3.910398 51085 51165 178-178 1.000000 49.6 ndhJ-ndhK_p3 ndhJ-ndhK 3 GCAATCGAAGCCCAAAGATC TCAGACAGAATTCTTTTGTGCA 20 22 50.0 36.4 57.5 56.5 2.1 4.9 3.998783 51085 51165 178-178 1.000000 48.9 ndhJ-ndhK_p4 ndhJ-ndhK 4 GTCCCAGTCCTCGGTTTTTA CAGACAGAATTCTTTTGTGCAGA 20 23 50.0 39.1 57.2 57.6 0.0 4.9 4.273858 51085 51165 219-219 1.000000 46.5 ndhJ-ndhK_p5 ndhJ-ndhK 5 GTCCCAGTCCTCGGTTTTTA TCAGACAGAATTCTTTTGTGCAG 20 23 50.0 39.1 57.2 57.6 0.0 4.9 4.273858 51085 51165 220-220 1.000000 46.5 ndhC-trnV_p1 ndhC-trnV 1 CGGGGCTAAAACTCCAGAAA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.391484 52287 53946 1775-1792 1.000000 79.6 ndhC-trnV_p2 ndhC-trnV 2 ATCGGGGCTAAAACTCCAGA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.4 57.8 0.0 0.0 0.617224 52287 53946 1777-1794 1.000000 77.8 ndhC-trnV_p3 ndhC-trnV 3 AATCGGGGCTAAAACTCCAG AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.6 57.8 0.0 0.0 0.617342 52287 53946 1778-1795 1.000000 77.8 ndhC-trnV_p4 ndhC-trnV 4 TCCTTCACTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.5 57.8 0.0 0.0 0.662079 52287 53946 1787-1804 1.000000 77.5 ndhC-trnV_p5 ndhC-trnV 5 CGGGGCTAAAACTCCAGAAA AGCCCAATGCAACAAAATGC 20 20 50.0 45.0 57.8 58.5 0.0 0.0 0.668261 52287 53946 1857-1874 1.000000 77.3 psbE-petL_p1 psbE-petL 1 TGACGAATAACCAACCTGCA AAGCAGCCAGTAGAAAACCG 20 20 45.0 50.0 57.4 58.5 0.0 0.0 1.028841 67028 68363 1344-1494 1.000000 76.9 psbE-petL_p2 psbE-petL 2 TGACGAATAACCAACCTGCA GCAGCCAGTAGAAAACCGAA 20 20 45.0 50.0 57.4 58.5 0.0 0.0 1.033191 67028 68363 1342-1492 1.000000 76.9 psbE-petL_p3 psbE-petL 3 CAAAAGAACGTTCTCCCGTG AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.139521 67028 68363 1260-1410 1.000000 76.1 psbE-petL_p4 psbE-petL 4 CAAAAGAACGTTCTCCCGTG GCAGCCAGTAGAAAACCGAA 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.143870 67028 68363 1258-1408 1.000000 76.0 psbE-petL_p5 psbE-petL 5 CAGCAAAAGAACGTTCTCCC AAGCAGCCAGTAGAAAACCG 20 20 50.0 50.0 57.3 58.5 29.8 0.0 1.164606 67028 68363 1263-1413 1.000000 75.9 psbT-psbN_p1 psbT-psbN 1 TTTTCGAGAACCGCCTAAGG ATCCATTCGAAGAACACGGG 20 20 50.0 50.0 57.9 58.0 0.0 3.3 0.113125 76780 76838 128-128 1.000000 81.6 psbT-psbN_p2 psbT-psbN 2 TTTTCGAGAACCGCCTAAGG TTCGAAGAACACGGGGACTA 20 20 50.0 50.0 57.9 58.4 0.0 0.0 0.469295 76780 76838 123-123 1.000000 78.8 psbT-psbN_p3 psbT-psbN 3 CGCCTAAGGTTCCGACTAAA ATCCATTCGAAGAACACGGG 20 20 50.0 50.0 57.3 58.0 0.0 3.3 0.684364 76780 76838 117-117 1.000000 77.3 psbT-psbN_p4 psbT-psbN 4 TTTTCGAGAACCGCCTAAGG TCGAAGAACACGGGGACTAG 20 20 50.0 55.0 57.9 58.8 0.0 0.0 0.917641 76780 76838 122-122 1.000000 75.3 psbT-psbN_p5 psbT-psbN 5 TTTTCGAGAACCGCCTAAGG CATTCGAAGAACACGGGGAC 20 20 50.0 55.0 57.9 58.9 0.0 3.3 1.012618 76780 76838 125-125 1.000000 74.5 rps8-rpl14_p1 rps8-rpl14 1 AACCGTTCCGTTTTTACCCA GAACTCGAGTTTTTGGTGCG 20 20 45.0 50.0 58.0 58.0 0.0 30.2 0.068605 83074 83275 360-367 1.000000 80.6 rps8-rpl14_p2 rps8-rpl14 2 AACCGTTCCGTTTTTACCCA GCTCGGGAATTGAGACAGTT 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.150493 83074 83275 337-344 1.000000 80.0 rps8-rpl14_p3 rps8-rpl14 3 AACCGTTCCGTTTTTACCCA ACTCGAGTTTTTGGTGCGAT 20 20 45.0 45.0 58.0 58.1 0.0 13.6 0.170935 83074 83275 358-365 1.000000 79.7 rps8-rpl14_p4 rps8-rpl14 4 ACCGTTCCGTTTTTACCCAT GAACTCGAGTTTTTGGTGCG 20 20 45.0 50.0 57.7 58.0 0.0 30.2 0.293851 83074 83275 359-366 1.000000 78.8 rps8-rpl14_p5 rps8-rpl14 5 CGAACCGTTCCGTTTTTACC GAACTCGAGTTTTTGGTGCG 20 20 50.0 50.0 57.7 58.0 5.7 30.2 0.317802 83074 83275 362-369 1.000000 78.6 rps3-rps19_p1 rps3-rps19 1 TGTAGACCCGCGGAAAAATT TCTGACTTTCGCGAGACATG 20 20 45.0 50.0 57.8 58.0 30.7 9.6 0.205450 85955 86444 640-650 1.000000 81.2 rps3-rps19_p2 rps3-rps19 2 TTGTAGACCCGCGGAAAAAT TCTGACTTTCGCGAGACATG 20 20 45.0 50.0 57.8 58.0 30.7 9.6 0.205450 85955 86444 641-651 1.000000 81.2 rps3-rps19_p3 rps3-rps19 3 TGTAGACCCGCGGAAAAATT TTGCGCCTACTCTGACTTTC 20 20 45.0 50.0 57.8 57.9 30.7 4.2 0.268348 85955 86444 650-660 1.000000 80.7 rps3-rps19_p4 rps3-rps19 4 TTGTAGACCCGCGGAAAAAT TTGCGCCTACTCTGACTTTC 20 20 45.0 50.0 57.8 57.9 30.7 4.2 0.268348 85955 86444 651-661 1.000000 80.7 rps3-rps19_p5 rps3-rps19 5 TGTAGACCCGCGGAAAAATT CCTACTCTGACTTTCGCGAG 20 20 45.0 55.0 57.8 57.8 30.7 9.6 0.369488 85955 86444 645-655 1.000000 79.9 trnH-rpl2_p1 trnH-rpl2 1 TGGATCAAGGCAGTGGATTG TTCTTCGTCGCCGTAAATAG 20 20 50.0 45.0 57.9 55.7 0.0 0.0 2.411520 86930 86972 122-122 1.000000 64.0 trnH-rpl2_p2 trnH-rpl2 2 GGATCAAGGCAGTGGATTGT TTCTTCGTCGCCGTAAATAG 20 20 50.0 45.0 57.9 55.7 0.0 0.0 2.413027 86930 86972 121-121 1.000000 64.0 trnH-rpl2_p3 trnH-rpl2 3 GTGGATCAAGGCAGTGGATT TTCTTCGTCGCCGTAAATAG 20 20 50.0 45.0 57.9 55.7 0.0 0.0 2.413027 86930 86972 123-123 1.000000 63.9 trnH-rpl2_p4 trnH-rpl2 4 CGGATGTAGCCAAGTGGATC TTCTTCGTCGCCGTAAATAG 20 20 55.0 45.0 58.1 55.7 0.2 0.0 2.407158 86930 86972 136-136 1.000000 63.7 trnH-rpl2_p5 trnH-rpl2 5 GATTGTGAATCCACCATGCG TTCTTCGTCGCCGTAAATAG 20 20 50.0 45.0 57.5 55.7 0.0 0.0 2.758462 86930 86972 107-107 1.000000 61.7 ndhF_p1 ndhF 1 AAGACCCTTCCCTTTGTTCG AGAATTGGTGAATCGGAAACA 20 21 50.0 38.1 57.7 55.8 0.0 0.0 3.487973 113307 115511 2465-2636 1.000000 53.8 ndhF_p2 ndhF 2 AATGCCGTCGCCTATTGTTA AGAATTGGTGAATCGGAAACA 20 21 45.0 38.1 57.7 55.8 0.0 0.0 3.535165 113307 115511 2605-2776 1.000000 53.4 ndhF_p3 ndhF 3 ATGCCGTCGCCTATTGTTAC AGAATTGGTGAATCGGAAACA 20 21 50.0 38.1 58.4 55.8 0.0 0.0 3.637447 113307 115511 2604-2775 1.000000 52.5 ndhF_p4 ndhF 4 TACCGAAGAAGACCCTTCCC AGAATTGGTGAATCGGAAACA 20 21 55.0 38.1 58.4 55.8 3.3 0.0 3.650962 113307 115511 2473-2644 1.000000 52.5 ndhF_p5 ndhF 5 ACCAAGTTCAACGTTAGCCA AGAATTGGTGAATCGGAAACA 20 21 45.0 38.1 57.7 55.8 0.0 0.0 3.551345 113307 115511 2863-2863 0.500000 31.6 ndhF-rpl32_p1 ndhF-rpl32 1 CTGCAATTGGTCGTGTGAAC TTGAATGTCCGGTGGAAACC 20 20 50.0 50.0 58.0 58.4 7.7 0.0 0.425038 115512 116476 1569-1609 1.000000 80.4 ndhF-rpl32_p2 ndhF-rpl32 2 TGTTGCTGTCGGAATAAGCA TTGAATGTCCGGTGGAAACC 20 20 45.0 50.0 57.8 58.4 7.1 0.0 0.565875 115512 116476 1154-1194 1.000000 80.4 ndhF-rpl32_p3 ndhF-rpl32 3 GGTCGTGTGAACCAAAAACC TTGAATGTCCGGTGGAAACC 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.586273 115512 116476 1561-1601 1.000000 79.2 ndhF-rpl32_p4 ndhF-rpl32 4 AGATTTAGCAACTGCACCGA TTGAATGTCCGGTGGAAACC 20 20 45.0 50.0 57.5 58.4 4.2 0.0 0.861177 115512 116476 1778-1818 1.000000 76.6 ndhF-rpl32_p5 ndhF-rpl32 5 CTGCAATTGGTCGTGTGAAC CGTCGAGATTCCAGGATTCA 20 20 50.0 50.0 58.0 57.1 7.7 12.9 0.907738 115512 116476 2188-2215 1.000000 75.9 psaC-ndhE_p1 psaC-ndhE 1 CAGGCTCGTACACATTGAGT ACTCGTATCAATCAATCGAATTTGT 20 25 50.0 32.0 57.6 57.5 0.0 0.0 5.909993 120436 120768 431-431 1.000000 43.9 psaC-ndhE_p2 psaC-ndhE 2 CAATACATCTGTGGGGCAGG ACTCGTATCAATCAATCGAATTTGT 20 25 55.0 32.0 58.3 57.5 0.0 0.0 5.855768 120436 120768 447-447 1.000000 43.8 psaC-ndhE_p3 psaC-ndhE 3 CCAATACATCTGTGGGGCAG ACTCGTATCAATCAATCGAATTTGT 20 25 55.0 32.0 58.3 57.5 0.0 0.0 5.855768 120436 120768 448-448 1.000000 43.8 psaC-ndhE_p4 psaC-ndhE 4 ACATCCGTCCCAAGGTATCA ACTCGTATCAATCAATCGAATTTGT 20 25 50.0 32.0 58.1 57.5 0.0 0.0 5.665582 120436 120768 471-471 1.000000 43.6 psaC-ndhE_p5 psaC-ndhE 5 AGCTTTACATCCGTCCCAAG ACTCGTATCAATCAATCGAATTTGT 20 25 50.0 32.0 57.6 57.5 0.0 0.0 5.942963 120436 120768 477-477 1.000000 43.5 rpl2-trnH_p1 rpl2-trnH 1 TTCTTCGTCGCCGTAAATAG TGGATCAAGGCAGTGGATTG 20 20 45.0 50.0 55.7 57.9 0.0 0.0 2.411520 157814 157856 122-122 1.000000 64.0 rpl2-trnH_p2 rpl2-trnH 2 TTCTTCGTCGCCGTAAATAG GGATCAAGGCAGTGGATTGT 20 20 45.0 50.0 55.7 57.9 0.0 0.0 2.413027 157814 157856 121-121 1.000000 64.0 rpl2-trnH_p3 rpl2-trnH 3 TTCTTCGTCGCCGTAAATAG GTGGATCAAGGCAGTGGATT 20 20 45.0 50.0 55.7 57.9 0.0 0.0 2.413027 157814 157856 123-123 1.000000 63.9 rpl2-trnH_p4 rpl2-trnH 4 TTCTTCGTCGCCGTAAATAG CGGATGTAGCCAAGTGGATC 20 20 45.0 55.0 55.7 58.1 0.0 0.2 2.407158 157814 157856 136-136 1.000000 63.7 rpl2-trnH_p5 rpl2-trnH 5 TTCTTCGTCGCCGTAAATAG GATTGTGAATCCACCATGCG 20 20 45.0 50.0 55.7 57.5 0.0 0.0 2.758462 157814 157856 107-107 1.000000 61.7