primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS(GCT)-trnR(TCT)_p1	trnS(GCT)-trnR(TCT)	1	ATTAGCAATCCGCCGCTTTA	AGAAGACCTCTGTCCTATCCA	20	21	45.0	47.6	58.0	56.9	0.0	0.0	2.089811	9108	10987	1922-1956	1.000000	65.1
trnS(GCT)-trnR(TCT)_p2	trnS(GCT)-trnR(TCT)	2	ATTAGCAATCCGCCGCTTTA	AGGTTTAGAAGACCTCTGTCCT	20	22	45.0	45.5	58.0	57.9	0.0	0.0	2.173139	9108	10987	1928-1962	1.000000	64.4
trnS(GCT)-trnR(TCT)_p3	trnS(GCT)-trnR(TCT)	3	CGGAGAGAGAGGGATTCGAA	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.3	56.9	0.0	0.0	2.370066	9108	10987	1970-2004	1.000000	62.8
trnS(GCT)-trnR(TCT)_p4	trnS(GCT)-trnR(TCT)	4	CGGAGAGAGAGGGATTCGAA	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.3	57.9	0.0	0.0	2.453394	9108	10987	1976-2010	1.000000	62.1
trnS(GCT)-trnR(TCT)_p5	trnS(GCT)-trnR(TCT)	5	GCTTTAGTCCACTCAGCCAT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	57.6	56.9	0.9	0.0	2.461553	9108	10987	1908-1942	1.000000	62.1
rpoB-trnC(GCA)_p1	rpoB-trnC(GCA)	1	CTTCCGTCAAGCCTTGATCA	TGAACTGGGGAGAAAGGGAT	20	20	50.0	50.0	57.9	57.9	10.9	0.0	0.158898	28171	29535	1521-1522	1.000000	79.0
rpoB-trnC(GCA)_p2	rpoB-trnC(GCA)	2	AGTTCTTCCGTCAAGCCTTG	TGAACTGGGGAGAAAGGGAT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175521	28171	29535	1525-1526	1.000000	78.9
rpoB-trnC(GCA)_p3	rpoB-trnC(GCA)	3	GTTCTTCCGTCAAGCCTTGA	TGAACTGGGGAGAAAGGGAT	20	20	50.0	50.0	58.1	57.9	2.2	0.0	0.180803	28171	29535	1524-1525	1.000000	78.9
rpoB-trnC(GCA)_p4	rpoB-trnC(GCA)	4	TGAAGTTCTTCCGTCAAGCC	TGAACTGGGGAGAAAGGGAT	20	20	50.0	50.0	58.1	57.9	4.2	0.0	0.180803	28171	29535	1528-1529	1.000000	78.9
rpoB-trnC(GCA)_p5	rpoB-trnC(GCA)	5	GAAGTTCTTCCGTCAAGCCT	TGAACTGGGGAGAAAGGGAT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.231097	28171	29535	1527-1528	1.000000	78.5
trnC(GCA)-petN_p1	trnC(GCA)-petN	1	TTTTGTATCGTTTTGGCGGC	TACCCCTAGAGTCCACTCCT	20	20	45.0	55.0	57.9	58.0	0.0	3.0	0.055942	29608	30659	1231-1233	1.000000	80.6
trnC(GCA)-petN_p2	trnC(GCA)-petN	2	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	20	20	45.0	45.0	57.9	58.0	0.0	0.0	0.059326	29608	30659	1170-1172	1.000000	80.6
trnC(GCA)-petN_p3	trnC(GCA)-petN	3	ATCCCTTTCTCCCCAGTTCA	TACCCCTAGAGTCCACTCCT	20	20	50.0	55.0	57.9	58.0	0.0	3.0	0.062416	29608	30659	1181-1183	1.000000	80.6
trnC(GCA)-petN_p4	trnC(GCA)-petN	4	ATCCCTTTCTCCCCAGTTCA	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.065800	29608	30659	1120-1122	1.000000	80.6
trnC(GCA)-petN_p5	trnC(GCA)-petN	5	TTTTGTATCGTTTTGGCGGC	GTACCCCTAGAGTCCACTCC	20	20	45.0	60.0	57.9	57.9	0.0	3.0	0.123587	29608	30659	1232-1234	1.000000	80.0
trnE(TTC)-trnT(GGT)_p1	trnE(TTC)-trnT(GGT)	1	AACTTCCCCTCGTAGTACCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.148006	33782	34604	951-971	1.000000	81.8
trnE(TTC)-trnT(GGT)_p2	trnE(TTC)-trnT(GGT)	2	ACGATCAACTTCCCCTCGTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.162602	33782	34604	957-977	1.000000	81.7
trnE(TTC)-trnT(GGT)_p3	trnE(TTC)-trnT(GGT)	3	AACTTCCCCTCGTAGTACCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.206103	33782	34604	930-950	1.000000	81.4
trnE(TTC)-trnT(GGT)_p4	trnE(TTC)-trnT(GGT)	4	ACGATCAACTTCCCCTCGTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.220699	33782	34604	936-956	1.000000	81.3
trnE(TTC)-trnT(GGT)_p5	trnE(TTC)-trnT(GGT)	5	AACTTCCCCTCGTAGTACCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.228257	33782	34604	927-947	1.000000	81.2
trnT(TGT)-trnL(TAA)_p1	trnT(TGT)-trnL(TAA)	1	TTCCGGGTCTAGGCCTTTTA	TACGTACGTACACGAGGTCA	20	20	50.0	50.0	58.0	57.9	12.6	23.6	0.117666	49709	50895	2037-2046	1.000000	80.0
trnT(TGT)-trnL(TAA)_p2	trnT(TGT)-trnL(TAA)	2	TTCCGGGTCTAGGCCTTTTA	AACACAGGATTTGGCTCAGG	20	20	50.0	50.0	58.0	58.1	12.6	0.0	0.129408	49709	50895	1885-1894	1.000000	80.0
trnT(TGT)-trnL(TAA)_p3	trnT(TGT)-trnL(TAA)	3	GGAAGTTCCCGCGACATAAT	TACGTACGTACACGAGGTCA	20	20	50.0	50.0	58.1	57.9	8.2	23.6	0.123651	49709	50895	1672-1686	1.000000	79.9
trnT(TGT)-trnL(TAA)_p4	trnT(TGT)-trnL(TAA)	4	TTCCGGGTCTAGGCCTTTTA	CGTACGTACACGAGGTCATC	20	20	50.0	55.0	58.0	57.9	12.6	24.5	0.127282	49709	50895	2035-2044	1.000000	79.9
trnT(TGT)-trnL(TAA)_p5	trnT(TGT)-trnL(TAA)	5	TTCCGGGTCTAGGCCTTTTA	CGAGGTCATCCTTTCTGTCG	20	20	50.0	55.0	58.0	58.1	12.6	0.0	0.129915	49709	50895	2025-2034	1.000000	79.9
trnF(GAA)-ndhJ_p1	trnF(GAA)-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.066573	51910	52621	886-903	1.000000	79.7
trnF(GAA)-ndhJ_p2	trnF(GAA)-ndhJ	2	CTCTAGCGGGATGATGCATC	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	58.0	58.1	20.1	0.0	0.071428	51910	52621	916-933	1.000000	79.7
trnF(GAA)-ndhJ_p3	trnF(GAA)-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.115963	51910	52621	850-867	1.000000	79.4
trnF(GAA)-ndhJ_p4	trnF(GAA)-ndhJ	4	TAGACCCAAGTCCTCTAGCG	ATCCACGCCTGAAACGTATC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.118300	51910	52621	928-945	1.000000	79.3
trnF(GAA)-ndhJ_p5	trnF(GAA)-ndhJ	5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.131276	51910	52621	858-875	1.000000	79.2
petA-psbJ_p1	petA-psbJ	1	GATCCATTACGCGTCCAAGT	CATATTCCGGATTGGGCTCA	20	20	50.0	50.0	58.1	57.4	18.6	4.1	0.630901	68311	69508	1347-1347	1.000000	76.4
petA-psbJ_p2	petA-psbJ	2	CGCGTCCAAGTTTTGTTGTT	CATATTCCGGATTGGGCTCA	20	20	45.0	50.0	58.1	57.4	0.0	4.1	0.663993	68311	69508	1338-1338	1.000000	76.1
petA-psbJ_p3	petA-psbJ	3	TCCATTACGCGTCCAAGTTT	CATATTCCGGATTGGGCTCA	20	20	45.0	50.0	57.8	57.4	18.6	4.1	0.739684	68311	69508	1345-1345	1.000000	75.5
petA-psbJ_p4	petA-psbJ	4	GATCCATTACGCGTCCAAGT	TATTCCGGATTGGGCTCATC	20	20	50.0	50.0	58.1	57.1	18.6	4.1	0.923233	68311	69508	1345-1345	1.000000	74.0
petA-psbJ_p5	petA-psbJ	5	GATCCATTACGCGTCCAAGT	TCATATTCCGGATTGGGCTC	20	20	50.0	50.0	58.1	57.1	18.6	4.1	0.923233	68311	69508	1348-1348	1.000000	74.0
rps18_p1	rps18	1	TCGGATCCGAAATGCATGAA	TGATTCAATTGAGGAGTAGGCC	20	22	45.0	45.5	57.7	57.8	34.0	8.4	2.480424	73704	74273	649-670	1.000000	63.4
rps18_p2	rps18	2	TCGGATCCGAAATGCATGAA	TTCAATTGAGGAGTAGGCCT	20	20	45.0	45.0	57.7	55.8	34.0	11.8	2.501827	73704	74273	646-667	1.000000	63.3
rps18_p3	rps18	3	GTCCTATTTCGGTCGGATCC	TGATTCAATTGAGGAGTAGGCC	20	22	55.0	45.5	57.6	57.8	0.0	8.4	2.591988	73704	74273	661-682	1.000000	62.5
rps18_p4	rps18	4	GTCCTATTTCGGTCGGATCC	TTCAATTGAGGAGTAGGCCT	20	20	55.0	45.0	57.6	55.8	0.0	11.8	2.613392	73704	74273	658-679	1.000000	62.3
rps18_p5	rps18	5	TCGGATCCGAAATGCATGAA	GATTCAATTGAGGAGTAGGCCT	20	22	45.0	45.5	57.7	57.6	34.0	11.8	2.753822	73704	74273	648-669	1.000000	61.2
ycf2-trnL(CAA)_p1	ycf2-trnL(CAA)	1	GGGATGGATTTTCCCGGAAG	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.3	58.0	0.7	15.9	0.315420	100610	101648	1184-1184	1.000000	77.7
ycf2-trnL(CAA)_p2	ycf2-trnL(CAA)	2	GGGATGGATTTTCCCGGAAG	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.3	58.0	0.7	15.9	0.316790	100610	101648	1186-1186	1.000000	77.7
ycf2-trnL(CAA)_p3	ycf2-trnL(CAA)	3	GGGATGGATTTTCCCGGAAG	TGGTGAAATGGTAGACACGC	20	20	55.0	50.0	58.3	58.2	0.7	0.0	0.504255	100610	101648	1189-1189	1.000000	76.2
ycf2-trnL(CAA)_p4	ycf2-trnL(CAA)	4	GGGATGGATTTTCCCGGAAG	GAAATGGTAGACACGCGAGA	20	20	55.0	50.0	58.3	57.7	0.7	15.9	0.580959	100610	101648	1185-1185	1.000000	75.6
ycf2-trnL(CAA)_p5	ycf2-trnL(CAA)	5	GGGATGGATTTTCCCGGAAG	GTTCGAGTCCTCTTCAAGGC	20	20	55.0	55.0	58.3	58.3	0.7	0.0	0.591596	100610	101648	1136-1136	1.000000	75.5
ndhA-ndhI_p1	ndhA-ndhI	1	TGACAACTTCTTCCCAACTCC	GCTCGTATTGTTTGTTGCCC	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.231559	126458	126534	162-162	1.000000	70.6
ndhA-ndhI_p2	ndhA-ndhI	2	CTTCCCAACTCCTTTCACTGT	GCTCGTATTGTTTGTTGCCC	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.237507	126458	126534	153-153	1.000000	70.6
ndhA-ndhI_p3	ndhA-ndhI	3	TGACAACTTCTTCCCAACTCC	CGATTCGCGTGAGATAAGGT	21	20	47.6	50.0	57.8	57.9	0.0	5.8	1.329864	126458	126534	213-213	1.000000	69.7
ndhA-ndhI_p4	ndhA-ndhI	4	CTTCCCAACTCCTTTCACTGT	CGATTCGCGTGAGATAAGGT	21	20	47.6	50.0	57.8	57.9	0.0	5.8	1.335812	126458	126534	204-204	1.000000	69.7
ndhA-ndhI_p5	ndhA-ndhI	5	TGACAACTTCTTCCCAACTCC	ACCAATGTACCTTGCAGCTC	21	20	47.6	50.0	57.8	58.2	0.0	5.4	1.382362	126458	126534	178-178	1.000000	69.4
ndhF_p1	ndhF	1	CATAAAGGGAGCGTCGTTCA	CCGTCTAGAGTATACCGGGG	20	20	50.0	60.0	58.0	58.2	11.3	6.3	0.185940	133123	135348	3000-3000	0.500000	58.5
ndhF_p2	ndhF	2	AAAGGGAGCGTCGTTCATAC	CCGTCTAGAGTATACCGGGG	20	20	50.0	60.0	58.0	58.2	11.3	6.3	0.187328	133123	135348	2997-2997	0.500000	58.5
ndhF_p3	ndhF	3	GGAGCGTCGTTCATACTTGT	CCGTCTAGAGTATACCGGGG	20	20	50.0	60.0	58.0	58.2	11.3	6.3	0.189188	133123	135348	2993-2993	0.500000	58.5
ndhF_p4	ndhF	4	CATAAAGGGAGCGTCGTTCA	GAACTGGGGAATCGGAAACA	20	20	50.0	50.0	58.0	57.8	11.3	0.0	0.198784	133123	135348	2587-2587	0.500000	58.4
ndhF_p5	ndhF	5	AAAGGGAGCGTCGTTCATAC	GAACTGGGGAATCGGAAACA	20	20	50.0	50.0	58.0	57.8	11.3	0.0	0.200172	133123	135348	2584-2584	0.500000	58.4
ndhF-trnL(TAG)_p1	ndhF-trnL(TAG)	1	GTCGATTAAGTGCCCGAACT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013801	135349	137231	2182-2182	0.500000	59.9
ndhF-trnL(TAG)_p2	ndhF-trnL(TAG)	2	GTCGATTAAGTGCCCGAACT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015171	135349	137231	2183-2183	0.500000	59.9
ndhF-trnL(TAG)_p3	ndhF-trnL(TAG)	3	GTCGATTAAGTGCCCGAACT	TTTTAGAGATGCCGCCACTC	20	20	50.0	50.0	58.0	58.0	0.0	6.0	0.030146	135349	137231	2242-2242	0.500000	59.8
ndhF-trnL(TAG)_p4	ndhF-trnL(TAG)	4	GTCGATTAAGTGCCCGAACT	CTTTTAGAGATGCCGCCACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035809	135349	137231	2243-2243	0.500000	59.7
ndhF-trnL(TAG)_p5	ndhF-trnL(TAG)	5	GTCGATTAAGTGCCCGAACT	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.0	58.0	0.0	1.0	0.048657	135349	137231	2792-2792	0.500000	59.6
trnL(CAA)-ycf2_p1	trnL(CAA)-ycf2	1	AAATGGTAGACACGCGAGAC	GGGATGGATTTTCCCGGAAG	20	20	50.0	55.0	58.0	58.3	15.9	0.7	0.315420	167613	168651	1184-1184	1.000000	77.7
trnL(CAA)-ycf2_p2	trnL(CAA)-ycf2	2	TGAAATGGTAGACACGCGAG	GGGATGGATTTTCCCGGAAG	20	20	50.0	55.0	58.0	58.3	15.9	0.7	0.316790	167613	168651	1186-1186	1.000000	77.7
trnL(CAA)-ycf2_p3	trnL(CAA)-ycf2	3	TGGTGAAATGGTAGACACGC	GGGATGGATTTTCCCGGAAG	20	20	50.0	55.0	58.2	58.3	0.0	0.7	0.504255	167613	168651	1189-1189	1.000000	76.2
trnL(CAA)-ycf2_p4	trnL(CAA)-ycf2	4	GAAATGGTAGACACGCGAGA	GGGATGGATTTTCCCGGAAG	20	20	50.0	55.0	57.7	58.3	15.9	0.7	0.580959	167613	168651	1185-1185	1.000000	75.6
trnL(CAA)-ycf2_p5	trnL(CAA)-ycf2	5	GTTCGAGTCCTCTTCAAGGC	GGGATGGATTTTCCCGGAAG	20	20	55.0	55.0	58.3	58.3	0.0	0.7	0.591596	167613	168651	1136-1136	1.000000	75.5