primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGGTATTTCTGGCTTTTCT	AATACGTGTGCTTGGGAGTC	20	20	40.0	50.0	54.5	57.9	0.0	0.0	3.586714	160	1221	1167-1167	1.000000	51.3
psbA_p2	psbA	2	TGGGTATTTCTGGCTTTTCTT	AATACGTGTGCTTGGGAGTC	21	20	38.1	50.0	55.4	57.9	0.0	0.0	3.728865	160	1221	1167-1167	1.000000	50.2
psbA_p3	psbA	3	TTGGGTATTTCTGGCTTTTCT	AATACGTGTGCTTGGGAGTC	21	20	38.1	50.0	55.4	57.9	0.0	0.0	3.728865	160	1221	1168-1168	1.000000	50.2
psbA_p4	psbA	4	TGGGTATTTCTGGCTTTTCTTTC	AATACGTGTGCTTGGGAGTC	23	20	39.1	50.0	57.3	57.9	0.0	0.0	3.816783	160	1221	1167-1167	1.000000	49.5
psbA_p5	psbA	5	TGGGTATTTCTGGCTTTTCTTT	AATACGTGTGCTTGGGAGTC	22	20	36.4	50.0	56.1	57.9	0.0	0.0	3.949738	160	1221	1167-1167	1.000000	48.4
matK-trnK-UUU_p1	matK-trnK-UUU	1	TTCCTGAAAGAGAAGTGGGT	AGAAGCGCCCAGTGTAATTC	20	20	45.0	50.0	56.0	58.3	0.0	2.3	2.284582	3307	3970	844-844	1.000000	61.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCCTGAAAGAGAAGTGGGTAGA	AGAAGCGCCCAGTGTAATTC	22	20	45.5	50.0	57.9	58.3	0.0	2.3	2.388851	3307	3970	843-843	1.000000	60.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCC	20	20	45.0	45.0	56.0	57.6	0.0	2.3	2.445924	3307	3970	855-855	1.000000	60.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCTGAAAGAGAAGTGGGTAGAC	AGAAGCGCCCAGTGTAATTC	22	20	50.0	50.0	57.8	58.3	0.0	2.3	2.456159	3307	3970	842-842	1.000000	60.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCC	22	20	45.5	45.0	57.9	57.6	0.0	2.3	2.550194	3307	3970	854-854	1.000000	59.6
rps16_p1	rps16	1	ATGTTGGATTGGCACGACAT	ACCACTTTGCAATGAAAAGAGG	20	22	45.0	40.9	58.2	57.7	4.1	23.2	2.488392	4549	5664	1259-1259	1.000000	60.1
rps16_p2	rps16	2	GCTTATGTTGGATTGGCACG	ACCACTTTGCAATGAAAAGAGG	20	22	50.0	40.9	57.8	57.7	0.0	23.2	2.519810	4549	5664	1263-1263	1.000000	59.8
rps16_p3	rps16	3	ATGTTGGATTGGCACGACAT	CCACTTTGCAATGAAAAGAGGA	20	22	45.0	40.9	58.2	57.4	4.1	21.2	2.751104	4549	5664	1258-1258	1.000000	58.0
rps16_p4	rps16	4	GCTTATGTTGGATTGGCACG	CCACTTTGCAATGAAAAGAGGA	20	22	50.0	40.9	57.8	57.4	0.0	21.2	2.782522	4549	5664	1262-1262	1.000000	57.7
rps16_p5	rps16	5	AGGGGCTTATGTTGGATTGG	ACCACTTTGCAATGAAAAGAGG	20	22	50.0	40.9	57.5	57.7	0.0	23.2	2.786059	4549	5664	1267-1267	1.000000	57.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5665	6278	687-687	1.000000	78.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5665	6278	698-698	1.000000	77.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	20	20	45.0	45.0	58.1	57.8	8.1	0.0	0.312175	5665	6278	756-756	1.000000	77.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5665	6278	691-691	1.000000	77.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.377084	5665	6278	767-767	1.000000	77.0
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	7599	8561	1108-1108	1.000000	78.3
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	TCCTACAAAAACGAAGCGGT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.281809	7599	8561	1099-1099	1.000000	77.7
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7599	8561	1079-1079	1.000000	77.2
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	GAGCGGAAAGAGAGGGATTC	GCCGGACCATCCTACAAAAA	20	20	55.0	50.0	57.8	58.2	0.0	5.0	0.403777	7599	8561	1159-1159	1.000000	76.8
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	GAGCGGAAAGAGAGGGATTC	TCCTACAAAAACGAAGCGGT	20	20	55.0	45.0	57.8	57.8	0.0	0.0	0.476358	7599	8561	1150-1150	1.000000	76.2
psbD_p1	psbD	1	GACATGAGGTGCTCGGAAAT	AGTGGTTTCTTGGTCACGAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.080373	8562	9623	1151-1151	1.000000	79.4
psbD_p2	psbD	2	GACATGAGGTGCTCGGAAAT	CAGTGGTTTCTTGGTCACGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.081734	8562	9623	1152-1152	1.000000	79.3
psbD_p3	psbD	3	GACATGAGGTGCTCGGAAAT	TCCAAGTAGCTTACCGGACA	20	20	50.0	50.0	58.0	58.1	0.0	9.5	0.102351	8562	9623	1211-1211	1.000000	79.2
psbD_p4	psbD	4	TTGGAAGTGACATGAGGTGC	AGTGGTTTCTTGGTCACGAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.154315	8562	9623	1159-1159	1.000000	78.8
psbD_p5	psbD	5	TTGGAAGTGACATGAGGTGC	CAGTGGTTTCTTGGTCACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.155676	8562	9623	1160-1160	1.000000	78.8
rpoB_p1	rpoB	1	GGGGGAGAACTTGCTTTAGG	TTCTGTCCAAATTGCGGAGT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.400624	21329	21448	235-235	1.000000	77.1
rpoB_p2	rpoB	2	GGGGGAGAACTTGCTTTAGG	TTCTACCCAGGATCCCAACC	20	20	55.0	55.0	57.9	58.4	0.0	8.6	0.538504	21329	21448	267-267	1.000000	76.0
rpoB_p3	rpoB	3	GGGGGAGAACTTGCTTTAGG	TCACCAGTTTCTACCCAGGA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.539336	21329	21448	275-275	1.000000	76.0
rpoB_p4	rpoB	4	GTTGGGGGAGAACTTGCTTT	TTCTGTCCAAATTGCGGAGT	20	20	50.0	45.0	58.3	57.7	0.0	0.0	0.565711	21329	21448	238-238	1.000000	75.8
rpoB_p5	rpoB	5	GGGGGAGAACTTGCTTTAGG	CTACCCAGGATCCCAACCTA	20	20	55.0	55.0	57.9	57.5	0.0	8.6	0.619800	21329	21448	265-265	1.000000	75.3
atpA_p1	atpA	1	TTCCCCTAGCTTCCGAAAAA	ACTTGTTTGCATGATTCAAATGA	20	23	45.0	30.4	56.7	55.9	0.0	0.0	6.352683	33945	35468	1654-1654	1.000000	40.0
atpA_p2	atpA	2	TTTCCCCTAGCTTCCGAAAA	ACTTGTTTGCATGATTCAAATGA	20	23	45.0	30.4	56.7	55.9	0.0	0.0	6.352683	33945	35468	1655-1655	1.000000	40.0
atpA_p3	atpA	3	TTTTCCCCTAGCTTCCGAAA	ACTTGTTTGCATGATTCAAATGA	20	23	45.0	30.4	56.7	55.9	0.0	0.0	6.352683	33945	35468	1656-1656	1.000000	40.0
atpA_p4	atpA	4	TTTTTCCCCTAGCTTCCGAA	ACTTGTTTGCATGATTCAAATGA	20	23	45.0	30.4	56.7	55.9	0.0	0.0	6.352683	33945	35468	1657-1657	1.000000	40.0
atpA_p5	atpA	5	TTCCCCTAGCTTCCGAAAAA	AGAAAACTTGTTTGCATGATTCA	20	23	45.0	30.4	56.7	55.8	0.0	0.0	6.414757	33945	35468	1659-1659	1.000000	40.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.104399	49527	50392	976-976	1.000000	79.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCTTCACTAACCGGTGCTA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.1	57.9	8.5	0.0	0.148492	49527	50392	999-999	1.000000	78.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ACTAACCGGTGCTAAAAGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.2	57.9	8.5	0.0	0.258622	49527	50392	993-993	1.000000	77.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.0	58.3	0.0	22.8	0.333780	49527	50392	977-977	1.000000	77.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.1	58.3	8.5	22.8	0.377873	49527	50392	1000-1000	1.000000	77.0
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	AGGATTCGAACCTACGACCA	AGCAAGTCTCCGTATCTTATTGG	20	23	50.0	43.5	58.2	58.0	15.0	0.0	3.206582	99947	101504	1735-1740	1.000000	54.6
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	GGATTCGAACCTACGACCAG	AGCAAGTCTCCGTATCTTATTGG	20	23	55.0	43.5	57.8	58.0	0.0	0.0	3.255257	99947	101504	1734-1739	1.000000	54.2
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	CCCCAAGTAGGATTCGAACC	AGCAAGTCTCCGTATCTTATTGG	20	23	55.0	43.5	57.7	58.0	0.0	0.0	3.377150	99947	101504	1743-1748	1.000000	53.2
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	AGGATTCGAACCTACGACCA	GCAAGTCTCCGTATCTTATTGGT	20	23	50.0	43.5	58.2	58.3	15.0	0.0	3.462293	99947	101504	1734-1739	1.000000	52.5
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	AGGATTCGAACCTACGACCA	GCAAGTCTCCGTATCTTATTGG	20	22	50.0	45.5	58.2	56.7	15.0	0.0	3.479410	99947	101504	1734-1739	1.000000	52.4
ndhH_p1	ndhH	1	ATCCCGTGAAATTCTCGAGC	AGTTGGTCGTATAATCGCGG	20	20	50.0	50.0	58.1	57.9	17.5	6.1	0.188498	101915	102121	1247-1255	1.000000	79.2
ndhH_p2	ndhH	2	ATCCCGTGAAATTCTCGAGC	GTTGGTCGTATAATCGCGGT	20	20	50.0	50.0	58.1	58.2	17.5	6.1	0.214683	101915	102121	1246-1254	1.000000	79.0
ndhH_p3	ndhH	3	ATCCCGTGAAATTCTCGAGC	ATGTAGGAGGAGGGCGAATT	20	20	50.0	50.0	58.1	58.2	17.5	0.0	0.250861	101915	102121	1081-1089	1.000000	78.8
ndhH_p4	ndhH	4	ATCCCGTGAAATTCTCGAGC	TCCCCTTTCCTATCCCCTTC	20	20	50.0	55.0	58.1	57.8	17.5	0.0	0.266601	101915	102121	976-984	1.000000	78.8
ndhH_p5	ndhH	5	ATCCCGTGAAATTCTCGAGC	AATGGAGCGACGGATAATGG	20	20	50.0	50.0	58.1	57.8	17.5	0.0	0.228507	101915	102121	1529-1537	1.000000	78.7
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	AGCAAGTCTCCGTATCTTATTGG	AGGATTCGAACCTACGACCA	23	20	43.5	50.0	58.0	58.2	0.0	15.0	3.206582	115284	116841	1735-1740	1.000000	54.6
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	AGCAAGTCTCCGTATCTTATTGG	GGATTCGAACCTACGACCAG	23	20	43.5	55.0	58.0	57.8	0.0	0.0	3.255257	115284	116841	1734-1739	1.000000	54.2
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	AGCAAGTCTCCGTATCTTATTGG	CCCCAAGTAGGATTCGAACC	23	20	43.5	55.0	58.0	57.7	0.0	0.0	3.377150	115284	116841	1743-1748	1.000000	53.2
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	GCAAGTCTCCGTATCTTATTGGT	AGGATTCGAACCTACGACCA	23	20	43.5	50.0	58.3	58.2	0.0	15.0	3.462293	115284	116841	1734-1739	1.000000	52.5
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	GCAAGTCTCCGTATCTTATTGG	AGGATTCGAACCTACGACCA	22	20	45.5	50.0	56.7	58.2	0.0	15.0	3.479410	115284	116841	1734-1739	1.000000	52.4