primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TGACTAAATCCCACAAAACA	GTCGTGCAGTGCAATTCAAT	20	20	35.0	45.0	52.6	57.7	0.0	21.7	5.751518	1387	4212	2883-2981	0.857143	40.4
trnK-UUU_p2	trnK-UUU	2	TGACTAAATCCCACAAAACAA	GTCGTGCAGTGCAATTCAAT	21	20	33.3	45.0	53.5	57.7	0.0	21.7	5.808922	1387	4212	2883-2981	0.857143	40.4
trnK-UUU_p3	trnK-UUU	3	ATGACTAAATCCCACAAAACA	GTCGTGCAGTGCAATTCAAT	21	20	33.3	45.0	53.3	57.7	0.0	21.7	6.043400	1387	4212	2884-2982	0.857143	40.4
trnK-UUU_p4	trnK-UUU	4	ATGACTAAATCCCACAAAACAA	GTCGTGCAGTGCAATTCAAT	22	20	31.8	45.0	54.2	57.7	0.0	21.7	6.172836	1387	4212	2884-2982	0.857143	40.4
trnK-UUU_p5	trnK-UUU	5	TGACTAAATCCCACAAAACAAT	GTCGTGCAGTGCAATTCAAT	22	20	31.8	45.0	54.2	57.7	0.0	21.7	6.172836	1387	4212	2883-2981	0.857143	40.4
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCTAAATATCCTTGTAATTCTTCCA	ACGGTAGAGTACTCGGCTTT	25	20	28.0	50.0	53.8	58.2	0.0	17.1	9.415305	3243	4175	886-1031	1.000000	48.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCTAAATATCCTTGTAATTCTTCCA	AACGGTAGAGTACTCGGCTT	25	20	28.0	50.0	53.8	58.2	0.0	17.1	9.415305	3243	4175	887-1032	1.000000	48.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTCTAAATATCCTTGTAATTCTTCCA	AACGGTAGAGTACTCGGCTT	26	20	26.9	50.0	54.5	58.2	0.0	17.1	9.687939	3243	4175	888-1033	1.000000	48.6
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTCTAAATATCCTTGTAATTCTTCCA	ACGGTAGAGTACTCGGCTTT	26	20	26.9	50.0	54.5	58.2	0.0	17.1	9.687939	3243	4175	887-1032	1.000000	48.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCTAAATATCCTTGTAATTCTTCCA	GGGTTGCTAACTCAACGGTA	25	20	28.0	50.0	53.8	57.5	0.0	0.0	9.707642	3243	4175	900-1045	1.000000	48.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	20	20	45.0	45.0	58.0	54.0	0.0	0.0	4.049283	8550	10040	749-1551	1.000000	59.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	TTACCACTAAACTATACCCGC	20	21	45.0	42.9	58.0	54.9	0.0	0.0	4.185542	8550	10040	750-1552	1.000000	58.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGAAGAAGCGGAGAGAGAGG	TACCACTAAACTATACCCGC	20	20	55.0	45.0	58.2	54.0	0.0	0.0	4.248369	8550	10040	805-1607	1.000000	57.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGGAGAGAGAGGGATTCGAA	TACCACTAAACTATACCCGC	20	20	55.0	45.0	58.3	54.0	0.0	0.0	4.329538	8550	10040	797-1599	1.000000	56.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGAAGAAGCGGAGAGAGAGG	TTACCACTAAACTATACCCGC	20	21	55.0	42.9	58.2	54.9	0.0	0.0	4.384628	8550	10040	806-1608	1.000000	56.1
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156700	14143	14687	661-705	1.000000	81.6
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAAGGAA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.3	58.0	0.0	0.0	0.695334	14143	14687	633-677	1.000000	77.4
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAAGGAAA	ATTTATGGACTGGTCGTGGC	21	20	47.6	50.0	58.1	58.0	0.0	0.0	1.094257	14143	14687	633-677	1.000000	74.2
atpF-atpH_p4	atpF-atpH	4	GTGACCCAAGGAAAGGAAAGA	ATTTATGGACTGGTCGTGGC	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.262061	14143	14687	631-675	1.000000	72.9
atpF-atpH_p5	atpF-atpH	5	CCAGTGACCCAAGGAAAGGA	ATTTATGGACTGGTCGTGGC	20	20	55.0	50.0	59.2	58.0	0.0	0.0	1.267260	14143	14687	634-678	1.000000	72.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	57.5	0.0	14.6	0.698720	27921	29156	1333-1384	1.000000	80.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.4	57.5	0.0	14.6	0.934514	27921	29156	1253-1304	1.000000	78.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.6	57.5	0.0	14.6	0.960477	27921	29156	1254-1305	1.000000	78.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.2	58.8	0.0	0.0	0.938794	27921	29156	1370-1421	1.000000	78.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AATTCTTCCGTCAAGCCCTG	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	58.2	58.8	0.0	0.7	1.008345	27921	29156	1356-1407	1.000000	77.6
petN-psbM_p1	petN-psbM	1	GTCTCACTTGGGCTGCTTTA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.592428	30431	30880	571-606	1.000000	73.3
petN-psbM_p2	petN-psbM	2	ACTTGGGCTGCTTTAATGGT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.7	57.6	0.0	0.0	1.716560	30431	30880	566-601	1.000000	72.3
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30431	30880	516-551	1.000000	72.2
petN-psbM_p4	petN-psbM	4	GTAAGTCTCACTTGGGCTGC	TGCTACTGCACTGTTCATTCT	20	21	55.0	42.9	58.6	57.6	0.0	0.0	1.963622	30431	30880	575-610	1.000000	70.3
petN-psbM_p5	petN-psbM	5	TAAGTCTCACTTGGGCTGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.6	57.6	0.0	0.0	2.055340	30431	30880	574-609	1.000000	69.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.315353	30986	31999	1114-1133	1.000000	63.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	TATGCCATACACCTTGCTGG	20	20	45.0	50.0	55.8	57.7	0.0	0.0	2.576065	30986	31999	1140-1159	1.000000	61.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	CCATACACCTTGCTGGGATT	20	20	45.0	50.0	55.8	57.6	0.0	2.7	2.671535	30986	31999	1136-1155	1.000000	60.6
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	22	20	40.9	50.0	57.4	57.9	0.0	0.0	2.709987	30986	31999	1121-1140	1.000000	60.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	20	20	45.0	50.0	55.8	57.5	0.0	2.7	2.726197	30986	31999	1131-1150	1.000000	60.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49882	50618	827-916	1.000000	81.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49882	50618	817-906	1.000000	80.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	49882	50618	873-962	1.000000	80.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49882	50618	870-959	1.000000	80.1
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49882	50618	869-958	1.000000	80.1
ndhK_p1	ndhK	1	CGATCCTGCTGCATCTTCTT	GGGGGATGCTTGGTTACAAT	20	20	50.0	50.0	58.0	57.8	1.3	0.0	0.208351	51189	52067	1073-1074	1.000000	79.2
ndhK_p2	ndhK	2	AAACGATCCTGCTGCATCTT	GGGGGATGCTTGGTTACAAT	20	20	45.0	50.0	57.9	57.8	1.3	0.0	0.278980	51189	52067	1076-1077	1.000000	78.7
ndhK_p3	ndhK	3	AACGATCCTGCTGCATCTTC	GGGGGATGCTTGGTTACAAT	20	20	50.0	50.0	58.3	57.8	1.3	0.0	0.497838	51189	52067	1075-1076	1.000000	76.9
ndhK_p4	ndhK	4	CGATCCTGCTGCATCTTCTT	CATGGGGGATGCTTGGTTAC	20	20	50.0	55.0	58.0	58.6	1.3	0.0	0.646385	51189	52067	1076-1077	1.000000	75.7
ndhK_p5	ndhK	5	AAACGATCCTGCTGCATCTT	CATGGGGGATGCTTGGTTAC	20	20	45.0	55.0	57.9	58.6	1.3	0.0	0.717014	51189	52067	1079-1080	1.000000	75.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	8.5	0.0	0.269796	52310	54404	1897-2260	1.000000	85.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	8.5	0.0	0.280450	52310	54404	1889-2252	1.000000	85.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	58.1	58.5	8.5	0.0	0.546572	52310	54404	1979-2342	1.000000	82.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	8.5	0.0	0.557226	52310	54404	1971-2334	1.000000	82.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	8.5	0.0	0.613568	52310	54404	1896-2259	1.000000	82.4
petA-psbJ_p1	petA-psbJ	1	AGGCCTTTTGCTCTTCTTGG	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.4	58.3	0.0	0.0	0.686927	65903	66933	1161-1172	1.000000	76.8
petA-psbJ_p2	petA-psbJ	2	AGGCCTTTTGCTCTTCTTGG	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.803033	65903	66933	1157-1168	1.000000	75.9
petA-psbJ_p3	petA-psbJ	3	AGGCCTTTTGCTCTTCTTGG	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.804737	65903	66933	1160-1171	1.000000	75.9
petA-psbJ_p4	petA-psbJ	4	TGCTCTTCTTGGCATCTGTT	GGTTCATATTCCGGGTTGGG	20	20	45.0	55.0	57.4	58.3	0.0	0.0	0.886443	65903	66933	1153-1164	1.000000	75.3
petA-psbJ_p5	petA-psbJ	5	TTGCTCTTCTTGGCATCTGT	GGTTCATATTCCGGGTTGGG	20	20	45.0	55.0	57.4	58.3	0.0	0.0	0.886443	65903	66933	1154-1165	1.000000	75.3
rpl33-rps18_p1	rpl33-rps18	1	CAGATCTAGGGCAGGATCCA	TGAGCTTGAGTTCCGATTGG	20	20	55.0	50.0	58.0	57.9	15.8	0.0	0.120403	71070	71424	1198-1281	1.000000	85.3
rpl33-rps18_p2	rpl33-rps18	2	AGATCTAGGGCAGGATCCAC	TGAGCTTGAGTTCCGATTGG	20	20	55.0	50.0	58.0	57.9	15.8	0.0	0.121859	71070	71424	1197-1280	1.000000	85.3
rpl33-rps18_p3	rpl33-rps18	3	CAGATCTAGGGCAGGATCCA	TAATCGATTCGATCCCCCGA	20	20	55.0	50.0	58.0	58.1	15.8	21.0	0.107484	71070	71424	935-1015	0.857143	79.9
rpl33-rps18_p4	rpl33-rps18	4	AGATCTAGGGCAGGATCCAC	TAATCGATTCGATCCCCCGA	20	20	55.0	50.0	58.0	58.1	15.8	21.0	0.108940	71070	71424	934-1014	0.857143	79.9
rpl33-rps18_p5	rpl33-rps18	5	GGCAGGATCCACGAAATCAT	TAATCGATTCGATCCCCCGA	20	20	50.0	50.0	58.0	58.1	15.8	21.0	0.113350	71070	71424	926-1006	0.857143	79.8
rps8-rpl14_p1	rps8-rpl14	1	CGGCAATATTGTCCCTACCC	GAACTCGAGTTTTTGGTGCG	20	20	55.0	50.0	58.1	58.0	14.3	30.2	0.126508	84024	84237	284-322	1.000000	84.5
rps8-rpl14_p2	rps8-rpl14	2	CGGCAATATTGTCCCTACCC	GCTCGGGAATTGAGACAGTT	20	20	55.0	50.0	58.1	57.9	14.3	0.0	0.208395	84024	84237	261-299	1.000000	84.0
rps8-rpl14_p3	rps8-rpl14	3	CGGCAATATTGTCCCTACCC	ACTCGAGTTTTTGGTGCGAT	20	20	55.0	45.0	58.1	58.1	14.3	13.6	0.228837	84024	84237	282-320	1.000000	83.7
rps8-rpl14_p4	rps8-rpl14	4	CGGCAATATTGTCCCTACCC	AACTCGAGTTTTTGGTGCGA	20	20	55.0	45.0	58.1	58.3	14.3	24.9	0.448797	84024	84237	283-321	1.000000	82.0
rps8-rpl14_p5	rps8-rpl14	5	CGGCAATATTGTCCCTACCC	GGAACTCGAGTTTTTGGTGC	20	20	55.0	50.0	58.1	57.6	14.3	30.2	0.508807	84024	84237	285-323	1.000000	81.5
rpl16_p1	rpl16	1	TCATCCTCAATATCCCTTTTGT	AGGTTGAATAAAAATTCGATTGACCA	22	26	36.4	30.8	54.8	58.1	0.0	0.0	11.278350	84738	86492	1792-1892	1.000000	47.3
rpl16_p2	rpl16	2	TCATCCTCAATATCCCTTTTGT	GGTTGAATAAAAATTCGATTGACCA	22	25	36.4	32.0	54.8	56.9	0.0	0.0	11.284229	84738	86492	1791-1891	1.000000	47.3
rpl16_p3	rpl16	3	TCCCTTTTGTTTAGTTCTACATCC	AGGTTGAATAAAAATTCGATTGACCA	24	26	37.5	30.8	56.6	58.1	0.0	0.0	11.493086	84738	86492	1780-1880	1.000000	47.3
rpl16_p4	rpl16	4	TCCCTTTTGTTTAGTTCTACATCC	GGTTGAATAAAAATTCGATTGACCA	24	25	37.5	32.0	56.6	56.9	0.0	0.0	11.498965	84738	86492	1779-1879	1.000000	47.3
rpl16_p5	rpl16	5	TCATCCTCAATATCCCTTTTGTT	AGGTTGAATAAAAATTCGATTGACCA	23	26	34.8	30.8	55.6	58.1	0.0	0.0	11.503805	84738	86492	1792-1892	1.000000	47.3
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	102979	104846	2007-2036	1.000000	80.1
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	102979	104846	1946-1975	1.000000	79.9
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	102979	104846	1950-1979	1.000000	79.8
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	102979	104846	1945-1974	1.000000	79.8
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	102979	104846	1948-1977	1.000000	78.9
ndhF-rpl32_p1	ndhF-rpl32	1	CATACGTGGAGGGGGAATTG	TCAGCGGGCTATGTCAATTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.079123	117224	117654	1325-1449	1.000000	85.6
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAACTGCACCGACAAATA	TCAGCGGGCTATGTCAATTC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.155565	117224	117654	1296-1420	1.000000	85.0
ndhF-rpl32_p3	ndhF-rpl32	3	ATTTAGCAACTGCACCGACA	TCAGCGGGCTATGTCAATTC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.155565	117224	117654	1300-1424	1.000000	85.0
ndhF-rpl32_p4	ndhF-rpl32	4	TAGCAACTGCACCGACAAAT	TCAGCGGGCTATGTCAATTC	20	20	45.0	50.0	58.1	58.0	4.2	0.0	0.155565	117224	117654	1297-1421	1.000000	85.0
ndhF-rpl32_p5	ndhF-rpl32	5	CATACGTGGAGGGGGAATTG	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.156959	117224	117654	1906-2214	1.000000	84.7
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	121558	122416	808-1078	1.000000	86.0
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	121558	122416	807-1077	1.000000	86.0
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	121558	122416	828-1098	1.000000	85.9
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	121558	122416	806-1076	1.000000	84.9
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	121558	122416	783-1053	1.000000	84.5