primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGCTCCATTGTTTGATTCAACG	GTGCTTGGGAGTCCTTGAAA	22	20	40.9	50.0	58.1	58.0	0.0	9.6	2.119009	151	1212	1152-1152	1.000000	63.2
psbA_p2	psbA	2	GCTCCATTGTTTGATTCAACGA	GTGCTTGGGAGTCCTTGAAA	22	20	40.9	50.0	57.8	58.0	0.0	9.6	2.178121	151	1212	1151-1151	1.000000	62.7
psbA_p3	psbA	3	TGCTCCATTGTTTGATTCAACG	AATACGTGTGCTTGGGAGTC	22	20	40.9	50.0	58.1	57.9	0.0	0.0	2.195028	151	1212	1159-1159	1.000000	62.6
psbA_p4	psbA	4	TGGGTATTGCTCCATTGTTTGA	GTGCTTGGGAGTCCTTGAAA	22	20	40.9	50.0	58.2	58.0	0.0	9.6	2.246356	151	1212	1159-1159	1.000000	62.2
psbA_p5	psbA	5	GCTCCATTGTTTGATTCAACGA	AATACGTGTGCTTGGGAGTC	22	20	40.9	50.0	57.8	57.9	0.0	0.0	2.254139	151	1212	1158-1158	1.000000	62.1
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATGATCCAGGGCGTAATCCT	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	58.3	58.0	0.0	9.8	0.303552	8213	8350	241-241	1.000000	78.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATGATCCAGGGCGTAATCCT	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.3	58.2	0.0	17.2	0.482274	8213	8350	196-196	1.000000	77.4
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATGATCCAGGGCGTAATCCT	GGATGGGAATGGGGAGAGAT	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.512738	8213	8350	273-273	1.000000	76.8
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	ATGATCCAGGGCGTAATCCT	TTCGAATCCCTCTCTCTCCG	20	20	50.0	55.0	58.3	58.3	0.0	0.0	0.583807	8213	8350	193-193	1.000000	76.6
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	AATGATCCAGGGCGTAATCC	TAAAGCGGCGGATTGCTAAT	20	20	50.0	45.0	57.4	58.0	0.0	9.8	0.606829	8213	8350	242-242	1.000000	76.1
trnS-GCU-trnG-UCU_p1	trnS-GCU-trnG-UCU	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8439	9791	1318-1427	1.000000	56.4
trnS-GCU-trnG-UCU_p2	trnS-GCU-trnG-UCU	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8439	9791	1321-1430	1.000000	55.3
trnS-GCU-trnG-UCU_p3	trnS-GCU-trnG-UCU	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8439	9791	1320-1429	1.000000	55.3
trnS-GCU-trnG-UCU_p4	trnS-GCU-trnG-UCU	4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.773135	8439	9791	1374-1483	1.000000	54.8
trnS-GCU-trnG-UCU_p5	trnS-GCU-trnG-UCU	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8439	9791	1319-1428	1.000000	54.5
trnG-UCU_p1	trnG-UCU	1	TGATTGCGCCTCACTCTTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	9792	10553	878-882	1.000000	49.6
trnG-UCU_p2	trnG-UCU	2	TTGATTGCGCCTCACTCTTT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	58.1	57.2	4.2	18.0	3.896810	9792	10553	879-883	1.000000	49.6
trnG-UCU_p3	trnG-UCU	3	GATTGCGCCTCACTCTTTTG	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	57.7	57.2	4.2	18.0	4.046422	9792	10553	877-881	1.000000	48.4
trnG-UCU_p4	trnG-UCU	4	ATGCTATCTTGATTGCGCCT	TGCATATCCAAATTGATGATGCA	20	23	45.0	34.8	57.7	57.2	4.2	18.0	4.060204	9792	10553	887-891	1.000000	48.3
trnG-UCU_p5	trnG-UCU	5	ATCTTGATTGCGCCTCACTC	TGCATATCCAAATTGATGATGCA	20	23	50.0	34.8	58.3	57.2	4.2	18.0	4.131689	9792	10553	882-886	1.000000	47.8
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49387	50109	833-851	1.000000	79.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49387	50109	823-841	1.000000	79.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	49387	50109	879-897	1.000000	78.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49387	50109	876-894	1.000000	78.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49387	50109	875-893	1.000000	78.3
accD-psaI_p1	accD-psaI	1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60638	61609	1085-1130	1.000000	81.5
accD-psaI_p2	accD-psaI	2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	60638	61609	1086-1131	1.000000	81.5
accD-psaI_p3	accD-psaI	3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60638	61609	1091-1136	1.000000	80.8
accD-psaI_p4	accD-psaI	4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.0	57.9	0.0	26.8	0.127877	60638	61609	1092-1137	1.000000	80.8
accD-psaI_p5	accD-psaI	5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	60638	61609	1091-1136	1.000000	80.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.350824	64719	65742	1176-1184	1.000000	79.6
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.467291	64719	65742	1159-1167	1.000000	78.7
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.466931	64719	65742	1172-1180	1.000000	78.6
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.468635	64719	65742	1175-1183	1.000000	78.6
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	64719	65742	1173-1181	1.000000	78.5
psbB_p1	psbB	1	AGTATAGTCCTTTCCAATGCGA	GGCGAAAGATACCAGAGCAA	22	20	40.9	50.0	57.0	58.0	0.0	0.0	3.045614	74649	76175	1626-1626	1.000000	56.0
psbB_p2	psbB	2	AGTATAGTCCTTTCCAATGCGA	GAGGCGAAAGATACCAGAGC	22	20	40.9	55.0	57.0	58.1	0.0	0.0	3.162055	74649	76175	1628-1628	1.000000	55.1
psbB_p3	psbB	3	AGTCCTTTCCAATGCGATAA	GGCGAAAGATACCAGAGCAA	20	20	40.0	50.0	54.7	58.0	0.0	0.0	3.288195	74649	76175	1621-1621	1.000000	54.1
psbB_p4	psbB	4	AGTCCTTTCCAATGCGATAA	GAGGCGAAAGATACCAGAGC	20	20	40.0	55.0	54.7	58.1	0.0	0.0	3.404636	74649	76175	1623-1623	1.000000	53.2
psbB_p5	psbB	5	AGTCCTTTCCAATGCGATAAA	GGCGAAAGATACCAGAGCAA	21	20	38.1	50.0	55.6	58.0	0.0	0.0	3.453074	74649	76175	1621-1621	1.000000	52.8
petD-rpoA_p1	petD-rpoA	1	TCGGTACCGTAGTAGCTCTT	ACACTCTACAGAAGTATTTCGCA	20	23	50.0	39.1	57.3	57.6	32.2	0.0	4.085812	80020	80211	352-375	1.000000	51.7
petD-rpoA_p2	petD-rpoA	2	ATCGGTACCGTAGTAGCTCT	ACACTCTACAGAAGTATTTCGCA	20	23	50.0	39.1	57.1	57.6	32.2	0.0	4.313735	80020	80211	353-376	1.000000	49.9
petD-rpoA_p3	petD-rpoA	3	TCGGTACCGTAGTAGCTCTTT	ACACTCTACAGAAGTATTTCGCA	21	23	47.6	39.1	58.0	57.6	32.2	0.0	4.395707	80020	80211	352-375	1.000000	49.3
petD-rpoA_p4	petD-rpoA	4	CGGTACCGTAGTAGCTCTTTG	ACACTCTACAGAAGTATTTCGCA	21	23	52.4	39.1	57.9	57.6	26.4	0.0	4.449281	80020	80211	351-374	1.000000	48.8
petD-rpoA_p5	petD-rpoA	5	ATCGGTACCGTAGTAGCTCTT	ACACTCTACAGAAGTATTTCGCA	21	23	47.6	39.1	57.8	57.6	32.2	0.0	4.592742	80020	80211	353-376	1.000000	47.7
rps8-rpl14_p1	rps8-rpl14	1	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	57.2	58.0	0.0	30.2	0.773663	82849	83028	347-350	1.000000	75.0
rps8-rpl14_p2	rps8-rpl14	2	GGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	20	20	55.0	50.0	58.8	58.0	0.0	30.2	0.809096	82849	83028	287-290	1.000000	74.9
rps8-rpl14_p3	rps8-rpl14	3	TGCTATTCGAACCGTTCCTT	GCTCGGGAATTGAGACAGTT	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.855551	82849	83028	324-327	1.000000	74.4
rps8-rpl14_p4	rps8-rpl14	4	GGCAATAGTGTCCCTACCCA	GCTCGGGAATTGAGACAGTT	20	20	55.0	50.0	58.8	57.9	0.0	0.0	0.890983	82849	83028	264-267	1.000000	74.4
rps8-rpl14_p5	rps8-rpl14	5	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	20	20	45.0	45.0	57.2	58.1	0.0	13.6	0.875993	82849	83028	345-348	1.000000	74.1
rpl22-rps19_p1	rpl22-rps19	1	TCTTCTCTACCTGAACTCCAGT	TACTTTCGCGAGACATGCAA	22	20	45.5	45.0	57.6	57.9	0.0	9.6	2.463246	86347	86487	217-223	1.000000	64.3
rpl22-rps19_p2	rpl22-rps19	2	TCTTCTCTACCTGAACTCCAGT	TTACTTTCGCGAGACATGCA	22	20	45.5	45.0	57.6	57.9	0.0	9.6	2.463246	86347	86487	218-224	1.000000	64.3
rpl22-rps19_p3	rpl22-rps19	3	TCTTCTCTACCTGAACTCCAGT	GAGAATTCGCGCCTACTCTT	22	20	45.5	50.0	57.6	57.8	0.0	0.0	2.601482	86347	86487	236-242	1.000000	62.9
rpl22-rps19_p4	rpl22-rps19	4	TCTTCTCTACCTGAACTCCAGT	TTCGCGAGACATGCAAGAAA	22	20	45.5	45.0	57.6	58.5	0.0	9.6	2.882567	86347	86487	213-219	1.000000	61.0
rpl22-rps19_p5	rpl22-rps19	5	TCTTCTCTACCTGAACTCCAGT	CAAATTGGGAGAATTCGCGC	22	20	45.5	50.0	57.6	58.5	0.0	17.5	2.841143	86347	86487	244-250	1.000000	60.8
rps19_p1	rps19	1	GCGAACCAAAAGGAACGATG	GGGTAGAGCCGGATCTAAGT	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.038031	86488	86766	1407-1413	1.000000	80.7
rps19_p2	rps19	2	GCGAACCAAAAGGAACGATG	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	58.0	58.0	0.0	29.9	0.039455	86488	86766	1408-1414	1.000000	80.6
rps19_p3	rps19	3	GCGAACCAAAAGGAACGATG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	22.4	0.060429	86488	86766	1270-1276	1.000000	80.6
rps19_p4	rps19	4	TGCTGCTGCAGAATAAACCA	GGGTAGAGCCGGATCTAAGT	20	20	45.0	55.0	58.1	58.0	32.3	29.9	0.093434	86488	86766	1092-1098	1.000000	80.5
rps19_p5	rps19	5	GCGAACCAAAAGGAACGATG	GAGGGTAAATCGGCCACATT	20	20	50.0	50.0	58.0	57.9	0.0	2.2	0.089035	86488	86766	1520-1526	1.000000	80.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGTTTCCACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.4	58.0	9.5	0.0	0.396312	116199	116987	881-884	1.000000	77.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGTTTCCACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.4	58.0	9.5	0.0	0.397682	116199	116987	882-885	1.000000	77.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTTTCCACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.4	57.9	9.5	0.0	0.512758	116199	116987	891-894	1.000000	77.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTTTCCACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.4	57.8	9.5	0.0	0.584776	116199	116987	890-893	1.000000	76.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGTTTCCACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.4	57.8	9.5	0.0	0.584776	116199	116987	889-892	1.000000	76.4
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120198	120797	725-812	1.000000	79.0
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	120198	120797	724-811	1.000000	79.0
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	120198	120797	745-832	1.000000	78.9
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	120198	120797	723-810	1.000000	77.9
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	120198	120797	700-787	1.000000	77.5
rps15-ycf1_p1	rps15-ycf1	1	AACGAGTTTTTGGTTCCCGA	AATGGCAGCCGTTTCAGTAT	20	20	45.0	45.0	58.0	57.9	0.0	13.7	0.170022	126496	126857	693-693	1.000000	80.0
rps15-ycf1_p2	rps15-ycf1	2	ACGAGTTTTTGGTTCCCGAA	AATGGCAGCCGTTTCAGTAT	20	20	45.0	45.0	58.0	57.9	0.0	13.7	0.170022	126496	126857	692-692	1.000000	80.0
rps15-ycf1_p3	rps15-ycf1	3	ACGAGTTTTTGGTTCCCGAA	ATAATGGCAGCCGTTTCAGT	20	20	45.0	45.0	58.0	57.9	0.0	24.3	0.170022	126496	126857	694-694	1.000000	80.0
rps15-ycf1_p4	rps15-ycf1	4	GGCTCAGCTCCAGCATAATT	AATGGCAGCCGTTTCAGTAT	20	20	50.0	45.0	58.0	57.9	14.7	13.7	0.150316	126496	126857	1129-1129	1.000000	79.9
rps15-ycf1_p5	rps15-ycf1	5	GGCTCAGCTCCAGCATAATT	ATAATGGCAGCCGTTTCAGT	20	20	50.0	45.0	58.0	57.9	14.7	24.3	0.150316	126496	126857	1131-1131	1.000000	79.9
ycf1_p1	ycf1	1	TTTCGGGGAGAACCAAAAGG	TCCATGCGTACTCAAAGACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019495	129912	130031	680-680	1.000000	82.3
ycf1_p2	ycf1	2	TTTCGGGGAGAACCAAAAGG	CTGGAAGAACTGGAGGAGGA	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.066117	129912	130031	896-896	1.000000	81.8
ycf1_p3	ycf1	3	TTTCGGGGAGAACCAAAAGG	TCTGGAAGAACTGGAGGAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.066117	129912	130031	897-897	1.000000	81.8
ycf1_p4	ycf1	4	TTTCGGGGAGAACCAAAAGG	TTCAAGAAAAGCCAAACGCG	20	20	50.0	45.0	58.0	58.2	0.0	3.5	0.176434	129912	130031	840-840	1.000000	80.9
ycf1_p5	ycf1	5	TTTCGGGGAGAACCAAAAGG	CCATGCGTACTCAAAGACGT	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.300281	129912	130031	679-679	1.000000	80.1