primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	ACCCTACCGAGGTTATGGAC	AGTCCTGAATTAGCTGCTGC	20	20	55.0	50.0	58.2	58.0	0.0	13.4	0.249883	4357	5108	917-935	1.000000	82.4
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	ACCCTACCGAGGTTATGGAC	CGAGCAAATGGAGTCCTGAA	20	20	55.0	50.0	58.2	57.9	0.0	11.3	0.313494	4357	5108	928-946	1.000000	81.8
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	CCTACCGAGGTTATGGACGA	AGTCCTGAATTAGCTGCTGC	20	20	55.0	50.0	58.3	58.0	0.0	13.4	0.351587	4357	5108	915-933	1.000000	81.6
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	CCGAGGTTATGGACGAAACA	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.6	58.0	0.0	13.4	0.409070	4357	5108	911-929	1.000000	81.2
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	ACCGAGGTTATGGACGAAAC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.6	58.0	0.0	13.4	0.410703	4357	5108	912-930	1.000000	81.1
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	TAGCGTCTACCAATTTCGCC	AAGAGAAAAGCCGGCTATCG	20	20	50.0	50.0	58.1	58.1	0.0	33.1	0.128108	14244	15146	1010-1036	1.000000	82.5
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	14244	15146	959-985	1.000000	82.1
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	AGTCCGTAGCGTCTACCAAT	AAGAGAAAAGCCGGCTATCG	20	20	50.0	50.0	58.2	58.1	10.2	33.1	0.298483	14244	15146	1016-1042	1.000000	81.1
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	14244	15146	991-1017	1.000000	81.1
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	14244	15146	965-991	1.000000	80.7
ycf3-psaA_p1	ycf3-psaA	1	ATTGCTGAGGCTTGGTTTGA	TGCCCATTCCTCGAAAGAAG	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.114212	18620	19575	1104-1151	1.000000	84.8
ycf3-psaA_p2	ycf3-psaA	2	TTGCTGAGGCTTGGTTTGAT	TGCCCATTCCTCGAAAGAAG	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.114212	18620	19575	1103-1150	1.000000	84.8
ycf3-psaA_p3	ycf3-psaA	3	ATTGCTGAGGCTTGGTTTGA	GCCCATTCCTCGAAAGAAGT	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.115695	18620	19575	1103-1150	1.000000	84.8
ycf3-psaA_p4	ycf3-psaA	4	TTGCTGAGGCTTGGTTTGAT	GCCCATTCCTCGAAAGAAGT	20	20	45.0	50.0	58.0	57.9	0.0	4.1	0.115695	18620	19575	1102-1149	1.000000	84.8
ycf3-psaA_p5	ycf3-psaA	5	TTGGTTGAAGATCACAGGGC	TGCCCATTCCTCGAAAGAAG	20	20	50.0	50.0	58.1	57.9	0.0	4.1	0.204161	18620	19575	1018-1065	1.000000	84.2
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GTTGGAGAGATGGCTGAGTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.0	58.4	0.0	3.5	0.443887	26342	26603	458-464	1.000000	81.1
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.0	58.4	0.0	3.5	0.443887	26342	26603	454-460	1.000000	81.0
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	ATAGCCCCGGTCTTGAAAAC	TAGGTCATTTATGGCACGCG	20	20	50.0	50.0	57.9	58.4	0.0	3.5	0.546809	26342	26603	434-440	1.000000	80.4
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GGGTTCGAATCCCTCTCTCT	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.2	58.4	17.2	3.5	0.639460	26342	26603	386-392	1.000000	80.2
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	TTTGGTTGGAGAGATGGCTG	TAGGTCATTTATGGCACGCG	20	20	50.0	50.0	57.8	58.4	0.0	3.5	0.627554	26342	26603	462-468	1.000000	79.6
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	CACAAAAACGAAACGGTCCC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	29035	30568	1519-1672	1.000000	82.9
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	CCACAAAAACGAAACGGTCC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	29035	30568	1520-1673	1.000000	82.8
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	CCCACAAAAACGAAACGGTC	GGCGTAAGTCATCGGTTCAA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.452720	29035	30568	1521-1674	1.000000	82.8
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	ACAAAAACGAAACGGTCCCT	GGCGTAAGTCATCGGTTCAA	20	20	45.0	50.0	58.2	58.3	0.0	0.0	0.528876	29035	30568	1518-1671	1.000000	82.2
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	AACCCACAAAAACGAAACGG	GGCGTAAGTCATCGGTTCAA	20	20	45.0	50.0	57.7	58.3	0.0	0.0	0.563947	29035	30568	1523-1676	1.000000	82.0
psbM-petN_p1	psbM-petN	1	TCATTCTAGTTCCCACTGCC	TCTTGCTTGGGCTGCTTTAA	20	20	50.0	45.0	56.9	58.0	0.0	0.0	1.086470	32771	33874	1234-1250	1.000000	75.2
psbM-petN_p2	psbM-petN	2	CATTCTAGTTCCCACTGCCT	TCTTGCTTGGGCTGCTTTAA	20	20	50.0	45.0	56.9	58.0	0.0	0.0	1.095003	32771	33874	1233-1249	1.000000	75.1
psbM-petN_p3	psbM-petN	3	TCATTCTAGTTCCCACTGCC	ATCTTGCTTGGGCTGCTTTA	20	20	50.0	45.0	56.9	57.8	0.0	0.0	1.301079	32771	33874	1235-1251	1.000000	73.5
psbM-petN_p4	psbM-petN	4	TCATTCTAGTTCCCACTGCC	TAAATCTTGCTTGGGCTGCT	20	20	50.0	45.0	56.9	57.8	0.0	0.0	1.301079	32771	33874	1238-1254	1.000000	73.4
psbM-petN_p5	psbM-petN	5	CATTCTAGTTCCCACTGCCT	ATCTTGCTTGGGCTGCTTTA	20	20	50.0	45.0	56.9	57.8	0.0	0.0	1.309611	32771	33874	1234-1250	1.000000	73.4
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CCGGATTTGAACTGGGGAAA	GACATTCCCGCATTTTCACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.360154	34380	35582	1288-1310	1.000000	80.1
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.423974	34380	35582	1352-1374	1.000000	79.4
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	ATTCTCTTGTCGATCAGGCG	GACATTCCCGCATTTTCACC	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.493933	34380	35582	1312-1334	1.000000	79.0
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	CCGGATTTGAACTGGGGAAA	GCCCCGATCAATGAACCTAC	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.487797	34380	35582	1349-1371	1.000000	78.9
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	ATTCTCTTGTCGATCAGGCG	TAAGCCCCGATCAATGAACC	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.557753	34380	35582	1376-1398	1.000000	78.3
rpoC2_p1	rpoC2	1	AGGATCTTCAAATTCCAATTAAGA	TATGTTGCTGCATGGATCGG	24	20	29.2	50.0	54.2	58.4	0.0	1.8	8.184286	44762	44881	218-218	1.000000	51.0
rpoC2_p2	rpoC2	2	AAAAGGATCTTCAAATTCCAAT	TATGTTGCTGCATGGATCGG	22	20	27.3	50.0	52.2	58.4	0.0	1.8	8.230548	44762	44881	221-221	1.000000	50.9
rpoC2_p3	rpoC2	3	AAAGGATCTTCAAATTCCAATT	TATGTTGCTGCATGGATCGG	22	20	27.3	50.0	52.2	58.4	0.0	1.8	8.230548	44762	44881	220-220	1.000000	50.9
rpoC2_p4	rpoC2	4	AAAAGGATCTTCAAATTCCAATT	TATGTTGCTGCATGGATCGG	23	20	26.1	50.0	53.1	58.4	0.0	1.8	8.342795	44762	44881	221-221	1.000000	50.9
rpoC2_p5	rpoC2	5	AAAAAGGATCTTCAAATTCCAA	TATGTTGCTGCATGGATCGG	22	20	27.3	50.0	52.4	58.4	0.0	1.8	8.001039	44762	44881	222-222	1.000000	50.8
atpI-atpH_p1	atpI-atpH	1	TACAAGCGGTATTCAAGCCC	AGCAGCAATCAGTGGATTCA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.534823	47877	49250	1423-1480	1.000000	81.0
atpI-atpH_p2	atpI-atpH	2	TACAAGCGGTATTCAAGCCC	CAGCAGCAATCAGTGGATTC	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.878720	47877	49250	1424-1481	1.000000	78.2
atpI-atpH_p3	atpI-atpH	3	CAAGCGGTATTCAAGCCCTT	AGCAGCAATCAGTGGATTCA	20	20	50.0	45.0	58.5	57.5	0.0	0.0	1.038468	47877	49250	1421-1478	1.000000	76.9
atpI-atpH_p4	atpI-atpH	4	TACAAGCGGTATTCAAGCCC	AGCAGCAATCAGTGGATTCAT	20	21	50.0	42.9	58.0	58.0	0.0	0.0	1.046984	47877	49250	1423-1480	1.000000	76.9
atpI-atpH_p5	atpI-atpH	5	TACAAGCGGTATTCAAGCCC	GCAGCAGCAATCAGTGGATT	20	20	50.0	50.0	58.0	59.2	0.0	0.0	1.216947	47877	49250	1425-1482	1.000000	75.5
trnR-UCU-trnS-GCU_p1	trnR-UCU-trnS-GCU	1	AGAAGACCTCTGTCCTATCCA	CAATCCGACGCTTTAGTCCA	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.056187	53254	55035	1822-1879	1.000000	67.9
trnR-UCU-trnS-GCU_p2	trnR-UCU-trnS-GCU	2	AGAAGACCTCTGTCCTATCCA	AATCCGACGCTTTAGTCCAC	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.057575	53254	55035	1821-1878	1.000000	67.9
trnR-UCU-trnS-GCU_p3	trnR-UCU-trnS-GCU	3	AGGTTTAGAAGACCTCTGTCCT	CAATCCGACGCTTTAGTCCA	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.139515	53254	55035	1828-1885	1.000000	67.2
trnR-UCU-trnS-GCU_p4	trnR-UCU-trnS-GCU	4	AGGTTTAGAAGACCTCTGTCCT	AATCCGACGCTTTAGTCCAC	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.140903	53254	55035	1827-1884	1.000000	67.2
trnR-UCU-trnS-GCU_p5	trnR-UCU-trnS-GCU	5	AGAAGACCTCTGTCCTATCCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	56.9	58.2	0.0	0.0	2.202953	53254	55035	1875-1932	1.000000	66.6
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	TTGGGTCTTCACTTGCACTG	20	20	55.0	50.0	58.1	58.3	16.9	0.0	0.396962	56684	58069	1470-1537	1.000000	82.9
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	ATTTCCGCCCTTACCACAAA	20	20	55.0	45.0	58.1	57.7	16.9	0.0	0.372174	56684	58069	1928-1995	1.000000	81.9
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	TTTCCGCCCTTACCACAAAT	20	20	55.0	45.0	58.1	57.7	16.9	0.0	0.372174	56684	58069	1927-1994	1.000000	81.9
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	TAAGGCATCGGGTTTTGGTC	ATTTCCGCCCTTACCACAAA	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.467094	56684	58069	1958-2025	1.000000	81.1
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	TAAGGCATCGGGTTTTGGTC	TTTCCGCCCTTACCACAAAT	20	20	50.0	45.0	58.2	57.7	0.0	0.0	0.467094	56684	58069	1957-2024	1.000000	81.1
psbE-petL_p1	psbE-petL	1	CAGCAAAAGAACGTTCTCCG	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.7	58.0	29.8	0.0	0.301053	64999	66353	1409-1430	1.000000	81.7
psbE-petL_p2	psbE-petL	2	CAGCAAAAGAACGTTCTCCG	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.7	58.0	29.8	0.0	0.306594	64999	66353	1411-1432	1.000000	81.6
psbE-petL_p3	psbE-petL	3	CAAAAGAACGTTCTCCGGTG	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.3	58.0	29.8	0.0	0.663708	64999	66353	1406-1427	1.000000	78.8
psbE-petL_p4	psbE-petL	4	CAAAAGAACGTTCTCCGGTG	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.3	58.0	29.8	0.0	0.669250	64999	66353	1408-1429	1.000000	78.7
psbE-petL_p5	psbE-petL	5	AGCAAAAGAACGTTCTCCGG	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	58.8	58.0	29.8	0.0	0.773683	64999	66353	1408-1429	1.000000	77.9
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	20	20	50.0	55.0	58.1	58.2	0.0	11.3	0.295154	67239	67673	512-544	1.000000	83.3
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	20	20	50.0	55.0	58.1	58.2	0.0	11.3	0.296497	67239	67673	511-543	1.000000	83.3
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	20	20	50.0	55.0	58.1	58.2	0.0	11.3	0.303190	67239	67673	523-555	1.000000	83.1
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	20	20	50.0	55.0	58.1	58.2	0.0	11.3	0.304523	67239	67673	522-554	1.000000	83.1
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	20	20	50.0	55.0	58.1	58.2	0.0	11.3	0.304523	67239	67673	525-557	1.000000	83.1
rps11-rpl36_p1	rps11-rpl36	1	CGTCCATTTTTACGCGAACC	CCAAGACATAAACAAAGACAAGGA	20	24	50.0	37.5	58.1	57.4	15.9	0.0	4.760205	80340	80913	503-651	1.000000	48.4
rps11-rpl36_p2	rps11-rpl36	2	CGTCCATTTTTACGCGAACC	TCCAAGACATAAACAAAGACAAGG	20	24	50.0	37.5	58.1	57.4	15.9	0.0	4.760205	80340	80913	504-652	1.000000	48.4
rps11-rpl36_p3	rps11-rpl36	3	CGTCCATTTTTACGCGAACC	TGTTCCAATCCAAGACATAAACA	20	23	50.0	34.8	58.1	56.4	15.9	0.0	4.763497	80340	80913	512-660	1.000000	48.4
rps11-rpl36_p4	rps11-rpl36	4	CGTCCATTTTTACGCGAACC	TGTTCCAATCCAAGACATAAACAA	20	24	50.0	33.3	58.1	57.0	15.9	0.0	5.094968	80340	80913	512-660	1.000000	46.5
rps11-rpl36_p5	rps11-rpl36	5	CGTCCATTTTTACGCGAACC	TTGTTCCAATCCAAGACATAAACA	20	24	50.0	33.3	58.1	57.0	15.9	0.0	5.094968	80340	80913	513-661	1.000000	46.5
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGAGCA	TCCTCTACAAACCATTCGAGC	20	21	50.0	47.6	58.4	57.8	0.0	0.0	1.649264	83187	84404	1441-1453	1.000000	69.2
rpl16-rps3_p2	rpl16-rps3	2	GTTCAAGTGCCTGAAGAGCA	CCTCTACAAACCATTCGAGCT	20	21	50.0	47.6	58.4	57.7	0.0	0.0	1.655232	83187	84404	1440-1452	1.000000	69.1
rpl16-rps3_p3	rpl16-rps3	3	GGGTTCAAGTGCCTGAAGAG	TCCTCTACAAACCATTCGAGC	20	21	55.0	47.6	58.5	57.8	0.0	0.0	1.718877	83187	84404	1443-1455	1.000000	68.6
rpl16-rps3_p4	rpl16-rps3	4	GGGTTCAAGTGCCTGAAGAG	CCTCTACAAACCATTCGAGCT	20	21	55.0	47.6	58.5	57.7	0.0	0.0	1.724845	83187	84404	1442-1454	1.000000	68.6
rpl16-rps3_p5	rpl16-rps3	5	TTCAAGTGCCTGAAGAGCAT	TCCTCTACAAACCATTCGAGC	20	21	45.0	47.6	57.4	57.8	0.0	0.0	1.821012	83187	84404	1440-1452	1.000000	67.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGAGAGGTTTTTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.143021	114660	115515	844-996	1.000000	88.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGAGAGGTTTTTGGACAGCA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.203748	114660	115515	837-989	1.000000	88.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGCAATCAATTTCTACGGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	0.3	0.0	0.229600	114660	115515	806-958	1.000000	88.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGAGAGGTTTTTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.215038	114660	115515	843-995	1.000000	88.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGAGAGGTTTTTGGACAGCA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.215038	114660	115515	842-994	1.000000	88.1
ycf1_p1	ycf1	1	ATCTAGAACTTCCGCCCTGT	GAGCACATTCTCCATAGGGC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.243092	130235	130354	1156-1156	0.875000	75.3
ycf1_p2	ycf1	2	ATCTAGAACTTCCGCCCTGT	AGGGCCCTCTTATCTCTTCC	20	20	50.0	55.0	58.1	57.9	0.0	22.3	0.243390	130235	130354	1141-1141	0.875000	75.3
ycf1_p3	ycf1	3	ATCTAGAACTTCCGCCCTGT	GGGCCCTCTTATCTCTTCCT	20	20	50.0	55.0	58.1	57.9	0.0	17.0	0.243390	130235	130354	1140-1140	0.875000	75.3
ycf1_p4	ycf1	4	ATCTAGAACTTCCGCCCTGT	TATGCAAGAAGGGACCGAGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.271996	130235	130354	1105-1105	0.875000	75.1
ycf1_p5	ycf1	5	ATCTAGAACTTCCGCCCTGT	CGAGCTCGGATTATGCAAGA	20	20	50.0	50.0	58.1	57.9	0.0	28.4	0.272702	130235	130354	1116-1116	0.875000	75.1