primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl16_p1	trnH-GUG-rpl16	1	TGAAATAGGATGGGCGAACG	CGAAATCGGATGCTTTTGGG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.245202	346	565	320-352	0.985714	86.7
trnH-GUG-rpl16_p2	trnH-GUG-rpl16	2	CAATCCACTGCCTTCATCCA	CGAAATCGGATGCTTTTGGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.326588	346	565	269-301	0.985714	86.7
trnH-GUG-rpl16_p3	trnH-GUG-rpl16	3	AATCCACTGCCTTCATCCAC	CGAAATCGGATGCTTTTGGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.328095	346	565	268-300	0.985714	86.7
trnH-GUG-rpl16_p4	trnH-GUG-rpl16	4	ACAATCCACTGCCTTCATCC	CGAAATCGGATGCTTTTGGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.328095	346	565	270-302	0.985714	86.7
trnH-GUG-rpl16_p5	trnH-GUG-rpl16	5	AAATAGGATGGGCGAACGAC	CGAAATCGGATGCTTTTGGG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.243841	346	565	318-350	0.985714	86.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	ATTTCCTTGAAAAAGGCGCG	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.232993	4733	5374	671-791	1.000000	87.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	ATTTCCTTGAAAAAGGCGCG	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.232993	4733	5374	670-790	1.000000	87.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTACCAACGGTATGGACGA	ATTTCCTTGAAAAAGGCGCG	20	20	50.0	45.0	57.9	57.9	10.6	0.0	0.191844	4733	5374	736-856	1.000000	87.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	ATTTCCTTGAAAAAGGCGCG	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.330411	4733	5374	685-805	1.000000	86.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	ATTTCCTTGAAAAAGGCGCG	20	20	50.0	45.0	58.3	57.9	5.3	0.0	0.347379	4733	5374	680-800	1.000000	86.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AGAACCCACAACCGATCATG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.189961	6511	7638	1217-1239	1.000000	83.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CCACAACCGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.189961	6511	7638	1212-1234	1.000000	83.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGAGCACCTCAGAATTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.6	16.9	0.176706	6511	7638	1260-1282	1.000000	83.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6511	7638	1191-1213	1.000000	83.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AACCCACAACCGATCATGTC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.254885	6511	7638	1215-1237	1.000000	83.3
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	58.0	57.6	0.0	0.0	1.413468	29678	30821	1287-1315	1.000000	71.7
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.8	57.6	0.0	0.0	1.598551	29678	30821	1293-1321	1.000000	70.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29678	30821	1233-1261	1.000000	69.0
petN-psbM_p4	petN-psbM	4	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.787488	29678	30821	1316-1344	1.000000	68.7
petN-psbM_p5	petN-psbM	5	AGGGGGCATAATTCACATGG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.794253	29678	30821	1317-1345	1.000000	68.6
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	55.8	58.5	0.0	5.4	2.706630	30927	32024	1190-1219	1.000000	63.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TTGACTGACCGTTTTTACGT	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	55.6	58.5	0.0	5.4	2.908127	30927	32024	1239-1268	1.000000	61.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.4	58.5	0.0	5.4	3.101264	30927	32024	1197-1226	1.000000	59.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TTTGACTGACCGTTTTTACGT	TTCAATTGGTCAGAGCACCG	21	20	38.1	50.0	56.3	58.5	0.0	5.4	3.129792	30927	32024	1240-1269	1.000000	59.5
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	55.8	57.0	0.0	5.4	3.226784	30927	32024	1191-1220	1.000000	58.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	TGTCTTCACGTTGGCTCATT	GGGAAAGAGCAATAGACCGG	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.299619	33447	34784	1525-1584	1.000000	81.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCGTAAGTCATCGGTTCAA	GGGAAAGAGCAATAGACCGG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.327879	33447	34784	1454-1513	1.000000	81.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	CCCCTTTAACTCAGCGGTAG	GGGAAAGAGCAATAGACCGG	20	20	55.0	55.0	57.7	58.0	0.0	0.0	0.356197	33447	34784	1490-1549	1.000000	81.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TGTCTTCACGTTGGCTCATT	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	57.7	58.2	0.0	0.0	0.500615	33447	34784	1497-1556	1.000000	80.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TGTCTTCACGTTGGCTCATT	GACCGGACCAACCTACAAAA	20	20	45.0	50.0	57.7	57.7	0.0	9.5	0.513812	33447	34784	1511-1570	1.000000	80.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TTTGATGCCCGAAAGTTGGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.063602	50164	50743	686-703	1.000000	84.2
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	50164	50743	679-696	1.000000	84.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	50164	50743	678-695	1.000000	84.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TATGATAATCACCCCCGCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.090759	50164	50743	715-732	1.000000	83.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CTAACATAGACCCCAGCCCT	TTTGATGCCCGAAAGTTGGA	20	20	55.0	45.0	58.2	58.0	0.0	0.0	0.203893	50164	50743	767-784	1.000000	82.6
clpP-rps12_p1	clpP-rps12	1	ATGCTGTAGCGGACGAAAAT	GAGCGGGGGATTTTGTTACA	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.195196	60511	61729	1324-2350	1.000000	84.4
clpP-rps12_p2	clpP-rps12	2	ATGCTGTAGCGGACGAAAAT	AGAGCGGGGGATTTTGTTAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.148453	60511	61729	1325-2351	0.985714	84.1
clpP-rps12_p3	clpP-rps12	3	GACTTGGACCTCCCATCCTA	AGAGCGGGGGATTTTGTTAC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300525	60511	61729	1296-2322	0.985714	83.0
clpP-rps12_p4	clpP-rps12	4	CTTGGACTTGGACTTGGACC	AGAGCGGGGGATTTTGTTAC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.226717	60511	61729	1373-2321	0.700000	71.1
clpP-rps12_p5	clpP-rps12	5	CTTGGACTTGGACTTGGACC	GAGCGGGGGATTTTGTTACA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.273461	60511	61729	1372-2320	0.700000	70.8
accD-trnG-GCC_p1	accD-trnG-GCC	1	CAACATCCAATTGAACCGCC	CTTCACGATAGGCGGATAGC	20	20	50.0	55.0	58.0	58.0	7.1	0.0	0.021112	73793	75133	1251-1482	1.000000	85.4
accD-trnG-GCC_p2	accD-trnG-GCC	2	CAATTGAACCGCCGTCTTTC	CTTCACGATAGGCGGATAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052281	73793	75133	1244-1475	1.000000	85.2
accD-trnG-GCC_p3	accD-trnG-GCC	3	AGCGATTTACTTAGCCCACG	CTTCACGATAGGCGGATAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.080095	73793	75133	1295-1526	1.000000	84.9
accD-trnG-GCC_p4	accD-trnG-GCC	4	ATTCCAATGGGGCCAATACC	CTTCACGATAGGCGGATAGC	20	20	50.0	55.0	57.9	58.0	4.4	0.0	0.117667	73793	75133	1319-1550	1.000000	84.6
accD-trnG-GCC_p5	accD-trnG-GCC	5	CAACATCCAATTGAACCGCC	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.0	57.8	7.1	1.1	0.174916	73793	75133	1217-1448	1.000000	84.2
psbT-psbN_p1	psbT-psbN	1	CGCCTAGGGTTCCAACTAAA	GATCCATTCGAGGAACACGG	20	20	50.0	55.0	57.2	58.4	11.7	0.0	1.202074	78572	78666	147-152	1.000000	76.8
psbT-psbN_p2	psbT-psbN	2	CGCCTAGGGTTCCAACTAAA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.2	57.7	11.7	0.0	1.058255	78572	78666	195-200	1.000000	76.4
psbT-psbN_p3	psbT-psbN	3	CGCCTAGGGTTCCAACTAAA	GTTTTACCGGGTACGCCTTA	20	20	50.0	50.0	57.2	57.6	11.7	6.3	1.152437	78572	78666	203-208	1.000000	75.5
psbT-psbN_p4	psbT-psbN	4	CGCCTAGGGTTCCAACTAAA	TGTCAGTTTTACCGGGTACG	20	20	50.0	50.0	57.2	57.6	11.7	6.3	1.211192	78572	78666	208-213	1.000000	74.9
psbT-psbN_p5	psbT-psbN	5	CGCCTAGGGTTCCAACTAAA	CGGGTACGCCTTATATACCG	20	20	50.0	55.0	57.2	57.5	11.7	4.1	1.323626	78572	78666	196-201	1.000000	74.3
rpl36-trnN-GUU_p1	rpl36-trnN-GUU	1	GTCTCTGTTTATGCCTCGGG	ATCTCCCCAGGTAGGATTCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.075580	84106	84760	823-840	1.000000	84.7
rpl36-trnN-GUU_p2	rpl36-trnN-GUU	2	TCTGTTTATGCCTCGGGTTG	ATCTCCCCAGGTAGGATTCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124855	84106	84760	820-837	1.000000	84.3
rpl36-trnN-GUU_p3	rpl36-trnN-GUU	3	CCTCGGGTTGGAGCAAATTA	ATCTCCCCAGGTAGGATTCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.139688	84106	84760	810-827	1.000000	84.3
rpl36-trnN-GUU_p4	rpl36-trnN-GUU	4	TTTTACGAATGGAAGCCCGT	ATCTCCCCAGGTAGGATTCG	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.204967	84106	84760	745-762	1.000000	84.2
rpl36-trnN-GUU_p5	rpl36-trnN-GUU	5	ACGGATCAGTCGGCATTTTT	ATCTCCCCAGGTAGGATTCG	20	20	45.0	55.0	58.2	58.0	0.0	0.0	0.195688	84106	84760	771-788	1.000000	84.1
trnN-GUU-infA_p1	trnN-GUU-infA	1	CCGTTGTTCCTCAGTAGCTC	GGGAGGATCTGCCGAAATTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.060924	84833	85351	474-709	1.000000	88.5
trnN-GUU-infA_p2	trnN-GUU-infA	2	CGAATTTGCCGTTGTTCCTC	GGGAGGATCTGCCGAAATTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094846	84833	85351	482-717	1.000000	88.4
trnN-GUU-infA_p3	trnN-GUU-infA	3	ATTTGCCGTTGTTCCTCAGT	GGGAGGATCTGCCGAAATTT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.077336	84833	85351	479-714	1.000000	88.3
trnN-GUU-infA_p4	trnN-GUU-infA	4	CCGTTGTTCCTCAGTAGCTC	TAGGTTATGGTTCGGGGAGG	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.205010	84833	85351	488-723	1.000000	87.3
trnN-GUU-infA_p5	trnN-GUU-infA	5	TGGGTTGAGAACGGGAATTG	GGGAGGATCTGCCGAAATTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.154306	84833	85351	515-750	0.971429	86.5
rpl16-rps3_p1	rpl16-rps3	1	AAGAGCGTATCCACCGAAAC	TCGAGATATCCATGGGGCAT	20	20	50.0	50.0	58.0	58.0	0.0	13.0	0.049102	87230	87388	330-336	1.000000	83.5
rpl16-rps3_p2	rpl16-rps3	2	GCCTCGATAAGATACCCCCT	TCGAGATATCCATGGGGCAT	20	20	55.0	50.0	58.1	58.0	0.0	13.0	0.103151	87230	87388	300-306	1.000000	83.4
rpl16-rps3_p3	rpl16-rps3	3	AAGAGCGTATCCACCGAAAC	CCTACAAACCATTCGAGCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010553	87230	87388	375-381	1.000000	83.3
rpl16-rps3_p4	rpl16-rps3	4	GCCTCGATAAGATACCCCCT	CCTACAAACCATTCGAGCGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064603	87230	87388	345-351	1.000000	83.2
rpl16-rps3_p5	rpl16-rps3	5	AGCGTATCCACCGAAACAAA	CCTACAAACCATTCGAGCGA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.177434	87230	87388	372-378	1.000000	82.0
rpl2-rpl23_p1	rpl2-rpl23	1	GCTCGGGGTAGAAGTTTTGT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.446269	90485	90531	133-201	1.000000	86.7
rpl2-rpl23_p2	rpl2-rpl23	2	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.444749	90485	90531	134-202	1.000000	86.6
rpl2-rpl23_p3	rpl2-rpl23	3	GTGCTCGGGGTAGAAGTTTT	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	57.8	58.3	0.0	13.0	0.446269	90485	90531	135-203	1.000000	86.6
rpl2-rpl23_p4	rpl2-rpl23	4	TGCTCGGGGTAGAAGTTTTG	ATCATTACGCTTCAACCGGG	20	20	50.0	50.0	57.8	58.3	0.0	6.3	0.515217	90485	90531	141-209	1.000000	85.8
rpl2-rpl23_p5	rpl2-rpl23	5	TTACCACAATGACGCTGTCC	CGCTTCAACCGGGTTATTCT	20	20	50.0	50.0	58.2	58.3	6.3	13.0	0.462503	90485	90531	213-281	1.000000	85.5
ycf1-ndhF_p1	ycf1-ndhF	1	ACGAAGGGGACTAAACAGGA	CACCCCTTGCTTGTTTTTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067615	117231	118009	1268-1398	1.000000	87.3
ycf1-ndhF_p2	ycf1-ndhF	2	ACGAAGGGGACTAAACAGGA	CCACCCCTTGCTTGTTTTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067615	117231	118009	1269-1399	1.000000	87.3
ycf1-ndhF_p3	ycf1-ndhF	3	ACAGGAACAAGAGGGATCCA	CACCCCTTGCTTGTTTTTGG	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.092600	117231	118009	1254-1384	1.000000	87.2
ycf1-ndhF_p4	ycf1-ndhF	4	ACGAAGGGGACTAAACAGGA	GGCTCTTATGGGCGGTTTAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066809	117231	118009	1351-1481	1.000000	86.8
ycf1-ndhF_p5	ycf1-ndhF	5	ACAGGAACAAGAGGGATCCA	GGCTCTTATGGGCGGTTTAA	20	20	50.0	50.0	58.0	58.0	15.2	0.0	0.091794	117231	118009	1337-1467	1.000000	86.7
ndhF-rpl32_p1	ndhF-rpl32	1	ATAGGAACTGGAAGGGGGAC	AACTTTTCGAACCCCCTTCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.114180	119498	120348	887-1112	0.971429	87.4
ndhF-rpl32_p2	ndhF-rpl32	2	GGAGAAGACCCACCCCTATT	AACTTTTCGAACCCCCTTCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.114180	119498	120348	910-1135	0.971429	87.4
ndhF-rpl32_p3	ndhF-rpl32	3	GAACAAGGAGAAGACCCACC	AACTTTTCGAACCCCCTTCC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.195411	119498	120348	916-1141	0.957143	86.1
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACAAGGAGAAGACCCAC	AACTTTTCGAACCCCCTTCC	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.195411	119498	120348	917-1142	0.957143	86.1
ndhF-rpl32_p5	ndhF-rpl32	5	AAGACCCACCCCTATTGCTA	AACTTTTCGAACCCCCTTCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.289187	119498	120348	906-1131	0.971429	86.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGTATGCGTAAAAATTTTTGGA	AGCGTGTCTACCGATTTCAC	22	20	27.3	50.0	52.9	58.0	23.4	0.0	7.069153	120385	120811	437-518	0.971429	48.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGTATGCGTAAAAATTTTTGGA	CAGCGTGTCTACCGATTTCA	22	20	27.3	50.0	52.9	58.0	23.4	0.0	7.070524	120385	120811	438-519	0.971429	48.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGTATGCGTAAAAATTTTTGGAA	AGCGTGTCTACCGATTTCAC	23	20	26.1	50.0	53.8	58.0	23.4	0.0	7.232964	120385	120811	437-518	0.971429	48.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGTATGCGTAAAAATTTTTGGA	TCCTAAGAGCAGCGTGTCTA	22	20	27.3	50.0	52.9	57.9	23.4	0.0	7.185600	120385	120811	447-528	0.971429	47.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGTATGCGTAAAAATTTTTGGA	TTATAAGATGCCGCCACTCG	22	20	27.3	50.0	52.9	57.9	23.4	0.0	7.210239	120385	120811	496-577	0.971429	47.5