primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	trnR-UCU-trnG-GCC	1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.081443	52315	52578	338-344	1.000000	69.2
trnR-UCU-trnG-GCC_p2	trnR-UCU-trnG-GCC	2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	21	20	47.6	55.0	56.9	58.1	0.0	0.0	2.110280	52315	52578	341-347	1.000000	68.9
trnR-UCU-trnG-GCC_p3	trnR-UCU-trnG-GCC	3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.164771	52315	52578	344-350	1.000000	68.4
trnR-UCU-trnG-GCC_p4	trnR-UCU-trnG-GCC	4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	22	20	45.5	55.0	57.9	58.1	0.0	0.0	2.193608	52315	52578	347-353	1.000000	68.2
trnR-UCU-trnG-GCC_p5	trnR-UCU-trnG-GCC	5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	21	20	47.6	55.0	56.9	57.7	0.0	0.0	2.385293	52315	52578	342-348	1.000000	66.7
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	TAGGCTTCGGGCAATTTAGC	GACCAAAACCCGTTGCCTTA	20	20	50.0	50.0	58.3	58.7	0.0	0.0	1.003941	54841	55287	574-583	1.000000	74.9
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	CGTAGGCTTCGGGCAATTTA	GACCAAAACCCGTTGCCTTA	20	20	50.0	50.0	58.3	58.7	5.5	0.0	1.020499	54841	55287	576-585	1.000000	74.7
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	TAGGCTTCGGGCAATTTAGC	GAATAGCGGGACCAAAACCC	20	20	50.0	55.0	58.3	58.9	0.0	0.0	1.223938	54841	55287	583-592	1.000000	73.1
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	CGTAGGCTTCGGGCAATTTA	GAATAGCGGGACCAAAACCC	20	20	50.0	55.0	58.3	58.9	5.5	0.0	1.240497	54841	55287	585-594	1.000000	72.9
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	TAGGCTTCGGGCAATTTAGC	ACCAAAACCCGTTGCCTTAC	20	20	50.0	50.0	58.3	59.0	0.0	0.0	1.290092	54841	55287	573-582	1.000000	72.6
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	CTTTATTCGTTGGGGCCGTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.029334	106305	106793	1389-1389	0.500000	59.8
ycf1_p2	ycf1	2	GAGCTACTGAGGAACAACGG	TACTTTATTCGTTGGGGCCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.029334	106305	106793	1391-1391	0.500000	59.8
ycf1_p3	ycf1	3	GAGCTACTGAGGAACAACGG	TTTATTCGTTGGGGCCGTAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.029334	106305	106793	1388-1388	0.500000	59.8
ycf1_p4	ycf1	4	GGTTAAATAGCCCCGGACTG	CTTTATTCGTTGGGGCCGTA	20	20	55.0	50.0	58.0	58.0	0.7	0.0	0.063831	106305	106793	1654-1654	0.500000	59.5
ycf1_p5	ycf1	5	GGTTAAATAGCCCCGGACTG	TTTATTCGTTGGGGCCGTAG	20	20	55.0	50.0	58.0	58.0	0.7	0.0	0.063831	106305	106793	1653-1653	0.500000	59.5
ndhF_p1	ndhF	1	TCGGCAATAATCACAAAAACGT	GTACGCTTTTTGGGAACTGC	22	20	36.4	50.0	57.5	58.0	0.0	0.0	2.537517	106758	109013	2941-2941	0.500000	39.7
ndhF_p2	ndhF	2	CGGCAATAATCACAAAAACGT	GTACGCTTTTTGGGAACTGC	21	20	38.1	50.0	56.1	58.0	0.0	0.0	2.980095	106758	109013	2940-2940	0.500000	36.2
ndhF_p3	ndhF	3	CAGTTCTTCGGCAATAATCACA	GTACGCTTTTTGGGAACTGC	22	20	40.9	50.0	57.1	58.0	0.0	0.0	2.991245	106758	109013	2948-2948	0.500000	36.1
ndhF_p4	ndhF	4	AACTGTCATAGCTCTACCCT	GTACGCTTTTTGGGAACTGC	20	20	45.0	50.0	54.9	58.0	0.0	0.0	3.103296	106758	109013	2979-2979	0.500000	35.2
ndhF_p5	ndhF	5	TTCGGCAATAATCACAAAAACGT	GTACGCTTTTTGGGAACTGC	23	20	34.8	50.0	58.1	58.0	0.0	0.0	3.185072	106758	109013	2942-2942	0.500000	34.5
ndhF-rpl32_p1	ndhF-rpl32	1	GATTTAGCGACTGAGCCACA	GTACGCTTTTTGGGAACTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056428	109014	109529	1261-1261	0.500000	59.5
ndhF-rpl32_p2	ndhF-rpl32	2	GATTTAGCGACTGAGCCACA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.134422	109014	109529	1895-1895	0.500000	58.9
ndhF-rpl32_p3	ndhF-rpl32	3	GATTTAGCGACTGAGCCACA	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.195149	109014	109529	1888-1888	0.500000	58.4
ndhF-rpl32_p4	ndhF-rpl32	4	ACATGAAGAGGGAATTGGGC	GTACGCTTTTTGGGAACTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.196256	109014	109529	1282-1282	0.500000	58.4
ndhF-rpl32_p5	ndhF-rpl32	5	GATTTAGCGACTGAGCCACA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.206440	109014	109529	1893-1893	0.500000	58.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCAGTTCCCAAAAAGCGTAC	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.135225	109692	110147	1135-1135	0.500000	58.9
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCAGTTCCCAAAAAGCGTAC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.170560	109692	110147	654-654	0.500000	58.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCAGTTCCCAAAAAGCGTAC	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.231287	109692	110147	647-647	0.500000	58.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAGTTCCCAAAAAGCGTAC	AAGACCCGAAGTAGCAAAGC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.236599	109692	110147	1142-1142	0.500000	58.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAGTTCCCAAAAAGCGTAC	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.242578	109692	110147	652-652	0.500000	58.1
ccsA_p1	ccsA	1	TAGACACGCTGCTCTTAGGA	TTTCGGCTGACAAGGTTGAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.169839	110318	111295	1446-1446	0.500000	58.6
ccsA_p2	ccsA	2	TAGACACGCTGCTCTTAGGA	TTTTCGGCTGACAAGGTTGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.169839	110318	111295	1447-1447	0.500000	58.6
ccsA_p3	ccsA	3	AAATTGGTAGACACGCTGCT	TTTCGGCTGACAAGGTTGAA	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.230565	110318	111295	1453-1453	0.500000	58.2
ccsA_p4	ccsA	4	AAATTGGTAGACACGCTGCT	TTTTCGGCTGACAAGGTTGA	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.230565	110318	111295	1454-1454	0.500000	58.2
ccsA_p5	ccsA	5	GTAGACACGCTGCTCTTAGG	TTTCGGCTGACAAGGTTGAA	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.241856	110318	111295	1447-1447	0.500000	58.1
ndhD_p1	ndhD	1	GATCGTTGGGGTTACCGTAC	AAATTGCTTCTGCCCCAAGA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.087448	111569	113068	2340-2340	0.500000	59.3
ndhD_p2	ndhD	2	GATCGTTGGGGTTACCGTAC	AATTGCTTCTGCCCCAAGAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.087448	111569	113068	2339-2339	0.500000	59.3
ndhD_p3	ndhD	3	GATCGTTGGGGTTACCGTAC	AAACAACTCGAAGCATGGGT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.092182	111569	113068	2238-2238	0.500000	59.3
ndhD_p4	ndhD	4	GATCGTTGGGGTTACCGTAC	GGCATGAAACAACTCGAAGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.104335	111569	113068	2244-2244	0.500000	59.2
ndhD_p5	ndhD	5	CTAATGAGGCATGGGGATCG	AATTGCTTCTGCCCCAAGAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.119016	111569	113068	2263-2263	0.500000	59.0
psaC_p1	psaC	1	TTTCTTTCCACCCCACATGG	ATCGGACCCGCTATTGTTTC	20	20	50.0	50.0	58.0	58.1	0.0	3.1	0.058777	113196	113441	1238-1238	0.500000	59.5
psaC_p2	psaC	2	AAAACAGAACTTCCCGCAGT	ATCGGACCCGCTATTGTTTC	20	20	45.0	50.0	57.9	58.1	0.0	3.1	0.103317	113196	113441	1291-1291	0.500000	59.2
psaC_p3	psaC	3	CGGTTGAGCTGCTAAGGTAG	ATCGGACCCGCTATTGTTTC	20	20	55.0	50.0	58.1	58.1	0.2	3.1	0.122640	113196	113441	1079-1079	0.500000	59.0
psaC_p4	psaC	4	TTTCTTTCCACCCCACATGG	CTATGGGTTGATCACGAGCC	20	20	50.0	55.0	58.0	58.1	0.0	8.4	0.137162	113196	113441	1410-1410	0.500000	58.9
psaC_p5	psaC	5	GTAACCGGTTGAGCTGCTAA	ATCGGACCCGCTATTGTTTC	20	20	50.0	50.0	57.9	58.1	30.1	3.1	0.139484	113196	113441	1084-1084	0.500000	58.9
ndhE_p1	ndhE	1	TCTTGGGGCAGAAGCAATTT	TGTCGTGACTCGCCAATAAG	20	20	45.0	50.0	58.0	58.0	0.0	1.2	0.023211	113746	114051	990-990	0.500000	59.8
ndhE_p2	ndhE	2	TTCTTGGGGCAGAAGCAATT	TGTCGTGACTCGCCAATAAG	20	20	45.0	50.0	58.0	58.0	0.0	1.2	0.023211	113746	114051	991-991	0.500000	59.8
ndhE_p3	ndhE	3	ACCCATGCTTCGAGTTGTTT	TGTCGTGACTCGCCAATAAG	20	20	45.0	50.0	58.0	58.0	0.0	1.2	0.027944	113746	114051	1092-1092	0.500000	59.8
ndhE_p4	ndhE	4	GCTTCGAGTTGTTTCATGCC	TGTCGTGACTCGCCAATAAG	20	20	50.0	50.0	58.0	58.0	0.0	1.2	0.040097	113746	114051	1086-1086	0.500000	59.7
ndhE_p5	ndhE	5	TCTTGGGGCAGAAGCAATTT	ACGTGGGCGCTATTAATGTT	20	20	45.0	45.0	58.0	58.0	0.0	7.6	0.046690	113746	114051	1306-1306	0.500000	59.6
ndhG_p1	ndhG	1	GAAACAATAGCGGGTCCGAT	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.1	57.9	14.1	0.0	0.148214	114281	114811	1541-1541	0.500000	58.8
ndhG_p2	ndhG	2	GAAACAATAGCGGGTCCGAT	CGACTTATGATCGCCACGAA	20	20	50.0	50.0	58.1	58.2	14.1	0.0	0.215414	114281	114811	1570-1570	0.500000	58.3
ndhG_p3	ndhG	3	GAAACAATAGCGGGTCCGAT	TTCGACTTATGATCGCCACG	20	20	50.0	50.0	58.1	58.2	14.1	0.0	0.215414	114281	114811	1572-1572	0.500000	58.3
ndhG_p4	ndhG	4	GGCTCGTGATCAACCCATAG	AATCAAATTGCTTTGGGCCG	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.226600	114281	114811	1369-1369	0.500000	58.2
ndhG_p5	ndhG	5	GGCTCGTGATCAACCCATAG	CGACTTATGATCGCCACGAA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.293799	114281	114811	1398-1398	0.500000	57.6
ndhG-ndhI_p1	ndhG-ndhI	1	AACATTAATAGCGCCCACGT	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	58.0	57.9	2.3	0.0	0.130360	114812	115272	752-752	0.500000	59.0
ndhG-ndhI_p2	ndhG-ndhI	2	AATCATGTAGTGGTTCGGGC	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.136067	114812	115272	564-564	0.500000	58.9
ndhG-ndhI_p3	ndhG-ndhI	3	AACATTAATAGCGCCCACGT	CGACTTATGATCGCCACGAA	20	20	45.0	50.0	58.0	58.2	2.3	0.0	0.197560	114812	115272	781-781	0.500000	58.4
ndhG-ndhI_p4	ndhG-ndhI	4	AACATTAATAGCGCCCACGT	TTCGACTTATGATCGCCACG	20	20	45.0	50.0	58.0	58.2	2.3	0.0	0.197560	114812	115272	783-783	0.500000	58.4
ndhG-ndhI_p5	ndhG-ndhI	5	AATCATGTAGTGGTTCGGGC	CGACTTATGATCGCCACGAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.203266	114812	115272	593-593	0.500000	58.4
ndhI_p1	ndhI	1	AACATTAATAGCGCCCACGT	TGATTTACCGGAAGCGGAAG	20	20	45.0	50.0	58.0	58.0	2.3	9.5	0.051222	115273	115758	1644-1644	0.500000	59.6
ndhI_p2	ndhI	2	AATCATGTAGTGGTTCGGGC	TGATTTACCGGAAGCGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	9.5	0.056929	115273	115758	1456-1456	0.500000	59.5
ndhI_p3	ndhI	3	AACATTAATAGCGCCCACGT	TTTACCGGAAGCGGAAGAAG	20	20	45.0	50.0	58.0	57.9	2.3	9.5	0.121630	115273	115758	1641-1641	0.500000	59.0
ndhI_p4	ndhI	4	AATCATGTAGTGGTTCGGGC	TTTACCGGAAGCGGAAGAAG	20	20	50.0	50.0	58.0	57.9	0.0	9.5	0.127336	115273	115758	1453-1453	0.500000	59.0
ndhI_p5	ndhI	5	AACATTAATAGCGCCCACGT	GGCCCGGTCCTTAGATTTTT	20	20	45.0	50.0	58.0	57.9	2.3	0.0	0.172004	115273	115758	1894-1894	0.500000	58.6
ndhI-ndhA_p1	ndhI-ndhA	1	CGGCCCAAAGCAATTTGATT	TGATTTACCGGAAGCGGAAG	20	20	45.0	50.0	57.9	58.0	0.0	9.5	0.116222	115759	115839	912-912	0.500000	59.1
ndhI-ndhA_p2	ndhI-ndhA	2	CGTGGCGATCATAAGTCGAA	TGATTTACCGGAAGCGGAAG	20	20	50.0	50.0	58.2	58.0	0.0	9.5	0.183421	115759	115839	881-881	0.500000	58.5
ndhI-ndhA_p3	ndhI-ndhA	3	TTCGTGGCGATCATAAGTCG	TGATTTACCGGAAGCGGAAG	20	20	50.0	50.0	58.2	58.0	0.0	9.5	0.183421	115759	115839	883-883	0.500000	58.5
ndhI-ndhA_p4	ndhI-ndhA	4	CGGCCCAAAGCAATTTGATT	TTTACCGGAAGCGGAAGAAG	20	20	45.0	50.0	57.9	57.9	0.0	9.5	0.186629	115759	115839	909-909	0.500000	58.5
ndhI-ndhA_p5	ndhI-ndhA	5	ACCAATGTACCTTGCAGCTC	TGATTTACCGGAAGCGGAAG	20	20	50.0	50.0	58.2	58.0	5.4	9.5	0.192157	115759	115839	550-550	0.500000	58.5
ndhA_p1	ndhA	1	CGGCCCAAAGCAATTTGATT	TTCGTCCACCGGGTTTTATC	20	20	45.0	50.0	57.9	57.9	0.0	6.3	0.195359	115840	118163	2959-2959	0.500000	58.4
ndhA_p2	ndhA	2	CGTGGCGATCATAAGTCGAA	TTCGTCCACCGGGTTTTATC	20	20	50.0	50.0	58.2	57.9	0.0	6.3	0.262559	115840	118163	2928-2928	0.500000	57.9
ndhA_p3	ndhA	3	TTCGTGGCGATCATAAGTCG	TTCGTCCACCGGGTTTTATC	20	20	50.0	50.0	58.2	57.9	0.0	6.3	0.262559	115840	118163	2930-2930	0.500000	57.9
ndhA_p4	ndhA	4	ACCAATGTACCTTGCAGCTC	TTCGTCCACCGGGTTTTATC	20	20	50.0	50.0	58.2	57.9	5.4	6.3	0.271295	115840	118163	2597-2597	0.500000	57.8
ndhA_p5	ndhA	5	GACCAATGTACCTTGCAGCT	TTCGTCCACCGGGTTTTATC	20	20	50.0	50.0	58.2	57.9	5.4	6.3	0.271295	115840	118163	2598-2598	0.500000	57.8
ndhH_p1	ndhH	1	TCAGGTCCAATACGCTGTTG	GAAAACCCAGGATCGGTTGA	20	20	50.0	50.0	57.9	57.8	0.0	4.5	0.287508	118165	119346	1701-1701	0.500000	57.7
ndhH_p2	ndhH	2	TCAGGTCCAATACGCTGTTG	AGAAAACCCAGGATCGGTTG	20	20	50.0	50.0	57.9	57.8	0.0	4.5	0.293679	118165	119346	1702-1702	0.500000	57.7
ndhH_p3	ndhH	3	CAACGAATCGCACGTAGAGA	GAAAACCCAGGATCGGTTGA	20	20	50.0	50.0	58.1	57.8	1.2	4.5	0.303023	118165	119346	2073-2073	0.500000	57.6
ndhH_p4	ndhH	4	CTCAACGAATCGCACGTAGA	GAAAACCCAGGATCGGTTGA	20	20	50.0	50.0	58.1	57.8	1.2	4.5	0.303023	118165	119346	2075-2075	0.500000	57.6
ndhH_p5	ndhH	5	TCAACGAATCGCACGTAGAG	GAAAACCCAGGATCGGTTGA	20	20	50.0	50.0	58.1	57.8	1.2	4.5	0.303023	118165	119346	2074-2074	0.500000	57.6
rps15-ycf1_p1	rps15-ycf1	1	AACACCATGCATTGATGGGT	GAACCAGTTCGCCTGTCTAG	20	20	45.0	55.0	58.1	58.0	8.9	0.0	0.060954	119719	120114	1377-1377	0.500000	59.5
rps15-ycf1_p2	rps15-ycf1	2	TAATCGTTGCCGTTTTCCCA	GAACCAGTTCGCCTGTCTAG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.108786	119719	120114	1119-1119	0.500000	59.1
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	TTATCCCCTAGGCGTCGTAG	20	20	45.0	55.0	58.1	58.1	8.9	2.1	0.157032	119719	120114	1152-1152	0.500000	58.7
rps15-ycf1_p4	rps15-ycf1	4	TCAACCGATCCTGGGTTTTC	GAACCAGTTCGCCTGTCTAG	20	20	50.0	55.0	57.8	58.0	1.1	0.0	0.196532	119719	120114	998-998	0.500000	58.4
rps15-ycf1_p5	rps15-ycf1	5	CAACCGATCCTGGGTTTTCT	GAACCAGTTCGCCTGTCTAG	20	20	50.0	55.0	57.8	58.0	1.1	0.0	0.202704	119719	120114	997-997	0.500000	58.4
ycf1_p1	ycf1	1	TTCTTTTCCCTGCCCAATCC	TCTTCGAATGACAACACGCA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.203426	120978	121097	491-491	0.500000	58.4
ycf1_p2	ycf1	2	CTACGACGCCTAGGGGATAA	TCTTCGAATGACAACACGCA	20	20	55.0	45.0	58.1	58.1	31.0	0.0	0.239026	120978	121097	1037-1037	0.500000	58.1
ycf1_p3	ycf1	3	TCCTTAAGCCCGGAAGAGTT	TCTTCGAATGACAACACGCA	20	20	50.0	45.0	58.3	58.1	0.7	0.0	0.487670	120978	121097	754-754	0.500000	56.1
ycf1_p4	ycf1	4	TTCCTTAAGCCCGGAAGAGT	TCTTCGAATGACAACACGCA	20	20	50.0	45.0	58.3	58.1	0.7	0.0	0.487670	120978	121097	755-755	0.500000	56.1
ycf1_p5	ycf1	5	CCATTCTTTTCCCTGCCCAA	TCTTCGAATGACAACACGCA	20	20	50.0	45.0	58.4	58.1	0.0	0.0	0.497307	120978	121097	494-494	0.500000	56.0